ID ARL8A_ARATH Reviewed; 184 AA. AC Q8VY57; Q9M2X1; DT 02-NOV-2016, integrated into UniProtKB/Swiss-Prot. DT 01-MAR-2002, sequence version 1. DT 12-APR-2017, entry version 123. DE RecName: Full=ADP-ribosylation factor-like protein 8a {ECO:0000303|PubMed:22174675}; DE Short=AtARL8a {ECO:0000303|PubMed:22174675}; DE AltName: Full=ADP-ribosylation factor-like A1C {ECO:0000303|PubMed:12644670}; DE Short=AtARLA1C {ECO:0000303|PubMed:12644670}; GN Name=ARL8A {ECO:0000303|PubMed:22174675}; GN Synonyms=ARLA1C {ECO:0000303|PubMed:12644670}; GN OrderedLocusNames=At3g49870 {ECO:0000312|Araport:AT3G49870}; GN ORFNames=T16K5.220 {ECO:0000312|EMBL:CAB66925.1}; OS Arabidopsis thaliana (Mouse-ear cress). OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliophyta; eudicotyledons; Gunneridae; OC Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; OC Arabidopsis. OX NCBI_TaxID=3702; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=cv. Columbia; RX PubMed=11130713; DOI=10.1038/35048706; RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., RA Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., RA Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., RA De Simone V., Choisne N., Artiguenave F., Robert C., Brottier P., RA Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., RA Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., RA Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., RA Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., RA Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., RA Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., RA Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., RA Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., RA Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., RA Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., RA Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., RA Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., RA Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., RA Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., RA Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., RA Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., RA Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., RA Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., RA Watanabe A., Yamada M., Yasuda M., Tabata S.; RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis RT thaliana."; RL Nature 408:820-822(2000). RN [2] RP GENOME REANNOTATION. RC STRAIN=cv. Columbia; RG The Arabidopsis Information Portal (Araport); RL Submitted (MAY-2016) to the EMBL/GenBank/DDBJ databases. RN [3] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RC STRAIN=cv. Columbia; RX PubMed=14593172; DOI=10.1126/science.1088305; RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., RA Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., RA Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G., RA Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., RA Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., RA Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., RA Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., RA Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., RA Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., RA Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., RA Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., RA Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.; RT "Empirical analysis of transcriptional activity in the Arabidopsis RT genome."; RL Science 302:842-846(2003). RN [4] RP GENE FAMILY, AND NOMENCLATURE. RX PubMed=12644670; DOI=10.1104/pp.013052; RA Vernoud V., Horton A.C., Yang Z., Nielsen E.; RT "Analysis of the small GTPase gene superfamily of Arabidopsis."; RL Plant Physiol. 131:1191-1208(2003). RN [5] RP GENE FAMILY. RX PubMed=15723828; DOI=10.1093/jxb/eri099; RA Gebbie L.K., Burn J.E., Hocart C.H., Williamson R.E.; RT "Genes encoding ADP-ribosylation factors in Arabidopsis thaliana; RT genome analysis and antisense suppression."; RL J. Exp. Bot. 56:1079-1091(2005). RN [6] RP FUNCTION (MICROBIAL INFECTION), DISRUPTION PHENOTYPE, GENE FAMILY, AND RP NOMENCLATURE. RC STRAIN=cv. Columbia; RX PubMed=22174675; DOI=10.1371/journal.ppat.1002409; RA Nishikiori M., Mori M., Dohi K., Okamura H., Katoh E., Naito S., RA Meshi T., Ishikawa M.; RT "A host small GTP-binding protein ARL8 plays crucial roles in RT tobamovirus RNA replication."; RL PLoS Pathog. 7:E1002409-E1002409(2011). CC -!- FUNCTION: May play a role in lysosome motility. May play a role in CC chromosome segregation. {ECO:0000250|UniProtKB:Q9NVJ2}. CC -!- FUNCTION: (Microbial infection) Component of tomato mosaic virus CC (ToMV) RNA replication complexes. Required for tobamovirus CC multiplication, especially for efficient negative-strand RNA CC synthesis and viral RNA capping. {ECO:0000269|PubMed:22174675}. CC -!- SUBUNIT: Interacts with tubulin. {ECO:0000250|UniProtKB:Q9NVJ2}. CC -!- SUBCELLULAR LOCATION: Late endosome membrane CC {ECO:0000250|UniProtKB:Q9NVJ2}. Lysosome membrane CC {ECO:0000250|UniProtKB:Q9NVJ2}. Cytoplasm, cytoskeleton, spindle CC {ECO:0000250|UniProtKB:Q9NVJ2}. Note=Localizes with microtubules CC at the spindle mid-zone during mitosis. CC {ECO:0000250|UniProtKB:Q9NVJ2}. CC -!- DISRUPTION PHENOTYPE: In double and triple mutants arl8a-1 arl8b-1 CC and arl8a-1 arl8b-1 arl8c-1, impaired multiplication of tomato CC mosaic virus (ToMV) associated with reduced production of CC negative-strand RNA. {ECO:0000269|PubMed:22174675}. CC -!- SIMILARITY: Belongs to the small GTPase superfamily. Arf family. CC {ECO:0000305}. CC -!- SEQUENCE CAUTION: CC Sequence=CAB66925.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305}; CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AL132965; CAB66925.1; ALT_SEQ; Genomic_DNA. DR EMBL; CP002686; AEE78600.1; -; Genomic_DNA. DR EMBL; AY072429; AAL62421.1; -; mRNA. DR EMBL; AY114718; AAM48037.1; -; mRNA. DR PIR; T46053; T46053. DR RefSeq; NP_190556.2; NM_114847.5. DR UniGene; At.35559; -. DR ProteinModelPortal; Q8VY57; -. DR SMR; Q8VY57; -. DR STRING; 3702.AT3G49870.1; -. DR PaxDb; Q8VY57; -. DR EnsemblPlants; AT3G49870.1; AT3G49870.1; AT3G49870. DR GeneID; 824149; -. DR Gramene; AT3G49870.1; AT3G49870.1; AT3G49870. DR KEGG; ath:AT3G49870; -. DR Araport; AT3G49870; -. DR TAIR; locus:2097355; AT3G49870. DR eggNOG; KOG0075; Eukaryota. DR eggNOG; ENOG410XNUK; LUCA. DR HOGENOM; HOG000163692; -. DR KO; K07955; -. DR OMA; FFQSGQF; -. DR OrthoDB; EOG09360NQX; -. DR PhylomeDB; Q8VY57; -. DR Reactome; R-ATH-6798695; Neutrophil degranulation. DR PRO; PR:Q8VY57; -. DR Proteomes; UP000006548; Chromosome 3. DR GO; GO:0005737; C:cytoplasm; IDA:TAIR. DR GO; GO:0005829; C:cytosol; IDA:TAIR. DR GO; GO:0005794; C:Golgi apparatus; IDA:TAIR. DR GO; GO:0031902; C:late endosome membrane; IEA:UniProtKB-SubCell. DR GO; GO:0005765; C:lysosomal membrane; IEA:UniProtKB-SubCell. DR GO; GO:0005634; C:nucleus; IDA:TAIR. DR GO; GO:0005886; C:plasma membrane; IDA:TAIR. DR GO; GO:0009506; C:plasmodesma; IDA:TAIR. DR GO; GO:0005819; C:spindle; IEA:UniProtKB-SubCell. DR GO; GO:0005774; C:vacuolar membrane; IDA:TAIR. DR GO; GO:0005773; C:vacuole; IDA:TAIR. DR GO; GO:0005525; F:GTP binding; ISS:TAIR. DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW. DR GO; GO:0007059; P:chromosome segregation; IEA:UniProtKB-KW. DR GO; GO:0051607; P:defense response to virus; IGI:TAIR. DR GO; GO:0007067; P:mitotic nuclear division; IEA:UniProtKB-KW. DR GO; GO:0007264; P:small GTPase mediated signal transduction; IEA:InterPro. DR InterPro; IPR027417; P-loop_NTPase. DR InterPro; IPR005225; Small_GTP-bd_dom. DR InterPro; IPR024156; Small_GTPase_ARF. DR InterPro; IPR006689; Small_GTPase_ARF/SAR. DR Pfam; PF00025; Arf; 1. DR PRINTS; PR00328; SAR1GTPBP. DR SUPFAM; SSF52540; SSF52540; 1. DR TIGRFAMs; TIGR00231; small_GTP; 1. DR PROSITE; PS51417; ARF; 1. PE 2: Evidence at transcript level; KW Cell cycle; Cell division; Chromosome partition; Complete proteome; KW Cytoplasm; Cytoskeleton; Endosome; GTP-binding; Lysosome; Membrane; KW Mitosis; Nucleotide-binding; Reference proteome. FT CHAIN 1 184 ADP-ribosylation factor-like protein 8a. FT /FTId=PRO_0000438003. FT INTRAMEM 1 18 Note=Mediates targeting to membranes. FT {ECO:0000250|UniProtKB:Q9NVJ2}. FT NP_BIND 29 34 GTP. {ECO:0000250|UniProtKB:P62330}. FT NP_BIND 48 51 GTP. {ECO:0000250|UniProtKB:P62330}. FT NP_BIND 70 74 GTP. {ECO:0000250|UniProtKB:P62330}. FT NP_BIND 129 132 GTP. {ECO:0000250|UniProtKB:P62330}. SQ SEQUENCE 184 AA; 20404 MW; F0492E876261BEF1 CRC64; MGLLEAFLNW LRSLFFKQEM ELSLIGLQNA GKTSLVNVVA TGGYSEDMIP TVGFNMRKVT KGNVTIKLWD LGGQPRFRSM WERYCRAVSA IVYVVDAADP DNLSVSKSEL HDLLSKTSLN GIPLLVLGNK IDKPGALSKE ALTDEMGLTS LTDREVCCFM ISCKNSTNID QVIDWLVKHS KSKN //