ID OR4M1_MOUSE Reviewed; 313 AA. AC Q8VFT4; DT 29-SEP-2021, integrated into UniProtKB/Swiss-Prot. DT 01-MAR-2002, sequence version 1. DT 24-JUL-2024, entry version 159. DE RecName: Full=Olfactory receptor 4M1 {ECO:0000305}; DE AltName: Full=Olfactory receptor 734 {ECO:0000303|PubMed:31230984}; GN Name=Or4m1 {ECO:0000312|MGI:MGI:3030568}; GN Synonyms=Olfr734 {ECO:0000303|PubMed:31230984, GN ECO:0000312|MGI:MGI:3030568}; OS Mus musculus (Mouse). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; OC Murinae; Mus; Mus. OX NCBI_TaxID=10090; RN [1] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RX PubMed=11875048; DOI=10.1093/hmg/11.5.535; RA Young J.M., Friedman C., Williams E.M., Ross J.A., Tonnes-Priddy L., RA Trask B.J.; RT "Different evolutionary processes shaped the mouse and human olfactory RT receptor gene families."; RL Hum. Mol. Genet. 11:535-546(2002). RN [2] RP ERRATUM OF PUBMED:11875048. RA Young J.M., Friedman C., Williams E.M., Ross J.A., Tonnes-Priddy L., RA Trask B.J.; RL Hum. Mol. Genet. 11:1683-1683(2002). RN [3] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RX PubMed=11802173; DOI=10.1038/nn800; RA Zhang X., Firestein S.; RT "The olfactory receptor gene superfamily of the mouse."; RL Nat. Neurosci. 5:124-133(2002). RN [4] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RX PubMed=14611657; DOI=10.1186/gb-2003-4-11-r71; RA Young J.M., Shykind B.M., Lane R.P., Tonnes-Priddy L., Ross J.A., RA Walker M., Williams E.M., Trask B.J.; RT "Odorant receptor expressed sequence tags demonstrate olfactory expression RT of over 400 genes, extensive alternate splicing and unequal expression RT levels."; RL Genome Biol. 4:R71.1-R71.13(2003). RN [5] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=C57BL/6J; RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112; RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S., RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., RA Eichler E.E., Ponting C.P.; RT "Lineage-specific biology revealed by a finished genome assembly of the RT mouse."; RL PLoS Biol. 7:E1000112-E1000112(2009). RN [6] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RC TISSUE=Brain {ECO:0000312|EMBL:AAI41030.1}; RX PubMed=15489334; DOI=10.1101/gr.2596504; RG The MGC Project Team; RT "The status, quality, and expansion of the NIH full-length cDNA project: RT the Mammalian Gene Collection (MGC)."; RL Genome Res. 14:2121-2127(2004). RN [7] RP FUNCTION, TISSUE SPECIFICITY, AND DISRUPTION PHENOTYPE. RX PubMed=31798959; DOI=10.1038/s41421-019-0122-x; RA Wei F., Long A., Wang Y.; RT "The Asprosin-OLFR734 hormonal signaling axis modulates male fertility."; RL Cell Discov. 5:55-55(2019). RN [8] RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE, RP AND MUTAGENESIS OF GLU-173 AND ARG-186. RX PubMed=31230984; DOI=10.1016/j.cmet.2019.05.022; RA Li E., Shan H., Chen L., Long A., Zhang Y., Liu Y., Jia L., Wei F., Han J., RA Li T., Liu X., Deng H., Wang Y.; RT "OLFR734 mediates glucose metabolism as a receptor of asprosin."; RL Cell Metab. 30:319-328(2019). RN [9] RP FUNCTION, AND DISRUPTION PHENOTYPE. RX PubMed=32337066; DOI=10.1038/s41421-020-0152-4; RA Liu Y., Long A., Chen L., Jia L., Wang Y.; RT "The Asprosin-OLFR734 module regulates appetitive behaviors."; RL Cell Discov. 6:19-19(2020). CC -!- FUNCTION: Olfactory receptor that acts as a receptor of Asprosin CC hormone at the surface of hepatocytes to promote hepatocyte glucose CC release (PubMed:31230984). Also binds Asprosin in the arcuate nucleus CC of the hypothalamus, thereby stimulating appetite by promoting CC orexigenic AgRP neuronal activity (PubMed:32337066). In testis, CC Asprosin-binding promotes sperm progressive motility and enhances male CC fertility (PubMed:31798959). The activity of this receptor is mediated CC by G proteins which activate adenylyl cyclase, resulting in an CC elevation of intracellular cAMP (PubMed:31230984). CC {ECO:0000269|PubMed:31230984, ECO:0000269|PubMed:31798959, CC ECO:0000269|PubMed:32337066}. CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:31230984}; CC Multi-pass membrane protein {ECO:0000255|RuleBase:RU363047}. CC -!- TISSUE SPECIFICITY: Highly expressed in liver but not in adipose tissue CC (PubMed:31230984). Also expressed at high level in testis CC (PubMed:31798959). {ECO:0000269|PubMed:31230984, CC ECO:0000269|PubMed:31798959}. CC -!- DISRUPTION PHENOTYPE: Compared to wild-type mice, mutant mice fed on a CC regular diet show a remarkable decrease in body weight, food intake, CC fat mass and plasma lipids (PubMed:31230984). Blunted response to CC Asprosin, including attenuated cAMP levels and hepatic glucose CC production, as well as improved insulin sensitivity (PubMed:31230984). CC Mutant mice show significantly reduced food intake in overnight-fasted CC mice compared with wild-type mice (PubMed:32337066). Male mice display CC decreased fertility caused by impaired sperm motility CC (PubMed:31798959). {ECO:0000269|PubMed:31230984, CC ECO:0000269|PubMed:31798959, ECO:0000269|PubMed:32337066}. CC -!- MISCELLANEOUS: The mouse olfactory receptor 4M1 (Q8VFT4) is not the one CC to one ortholog of human OR4M1 (Q8NGD0). {ECO:0000305}. CC -!- SIMILARITY: Belongs to the G-protein coupled receptor 1 family. CC {ECO:0000305}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AY073432; AAL61095.1; -; Genomic_DNA. DR EMBL; AY317859; AAP71197.1; -; Genomic_DNA. DR EMBL; BC141029; AAI41030.1; -; mRNA. DR CCDS; CCDS27010.1; -. DR RefSeq; NP_666875.1; NM_146664.1. DR AlphaFoldDB; Q8VFT4; -. DR SMR; Q8VFT4; -. DR STRING; 10090.ENSMUSP00000150732; -. DR GlyCosmos; Q8VFT4; 1 site, No reported glycans. DR GlyGen; Q8VFT4; 1 site. DR PaxDb; 10090-ENSMUSP00000057376; -. DR Ensembl; ENSMUST00000050928.3; ENSMUSP00000057376.3; ENSMUSG00000045306.4. DR Ensembl; ENSMUST00000217152.2; ENSMUSP00000150732.2; ENSMUSG00000045306.4. DR GeneID; 258658; -. DR KEGG; mmu:258658; -. DR UCSC; uc007tkx.1; mouse. DR AGR; MGI:3030568; -. DR CTD; 441670; -. DR MGI; MGI:3030568; Or4m1. DR VEuPathDB; HostDB:ENSMUSG00000045306; -. DR eggNOG; ENOG502SKDS; Eukaryota. DR GeneTree; ENSGT00940000163142; -. DR HOGENOM; CLU_012526_8_1_1; -. DR InParanoid; Q8VFT4; -. DR OMA; MNRHLCC; -. DR OrthoDB; 4230545at2759; -. DR PhylomeDB; Q8VFT4; -. DR TreeFam; TF338273; -. DR BioGRID-ORCS; 258658; 1 hit in 70 CRISPR screens. DR PRO; PR:Q8VFT4; -. DR Proteomes; UP000000589; Chromosome 14. DR RNAct; Q8VFT4; Protein. DR Bgee; ENSMUSG00000045306; Expressed in respiratory tract epithelium and 4 other cell types or tissues. DR GO; GO:0016020; C:membrane; ISA:MGI. DR GO; GO:0005886; C:plasma membrane; IDA:UniProtKB. DR GO; GO:0004930; F:G protein-coupled receptor activity; IEA:UniProtKB-KW. DR GO; GO:0004984; F:olfactory receptor activity; IDA:UniProtKB. DR GO; GO:0007189; P:adenylate cyclase-activating G protein-coupled receptor signaling pathway; IMP:UniProtKB. DR GO; GO:0007186; P:G protein-coupled receptor signaling pathway; ISA:MGI. DR GO; GO:0042593; P:glucose homeostasis; IDA:UniProtKB. DR GO; GO:0032100; P:positive regulation of appetite; IMP:UniProtKB. DR GO; GO:1902093; P:positive regulation of flagellated sperm motility; IMP:UniProtKB. DR GO; GO:0007608; P:sensory perception of smell; ISA:MGI. DR CDD; cd15937; 7tmA_OR4N-like; 1. DR Gene3D; 1.20.1070.10; Rhodopsin 7-helix transmembrane proteins; 1. DR InterPro; IPR000276; GPCR_Rhodpsn. DR InterPro; IPR017452; GPCR_Rhodpsn_7TM. DR InterPro; IPR000725; Olfact_rcpt. DR InterPro; IPR050427; Olfactory_Receptors. DR PANTHER; PTHR48002; OLFACTORY RECEPTOR; 1. DR PANTHER; PTHR48002:SF103; OLFACTORY RECEPTOR 4M1-RELATED; 1. DR Pfam; PF13853; 7tm_4; 1. DR PRINTS; PR00237; GPCRRHODOPSN. DR PRINTS; PR00245; OLFACTORYR. DR SUPFAM; SSF81321; Family A G protein-coupled receptor-like; 1. DR PROSITE; PS00237; G_PROTEIN_RECEP_F1_1; 1. DR PROSITE; PS50262; G_PROTEIN_RECEP_F1_2; 1. PE 1: Evidence at protein level; KW Cell membrane; Disulfide bond; G-protein coupled receptor; Glycoprotein; KW Membrane; Olfaction; Receptor; Reference proteome; Sensory transduction; KW Transducer; Transmembrane; Transmembrane helix. FT CHAIN 1..313 FT /note="Olfactory receptor 4M1" FT /id="PRO_0000453783" FT TOPO_DOM 1..25 FT /note="Extracellular" FT /evidence="ECO:0000305" FT TRANSMEM 26..46 FT /note="Helical; Name=1" FT /evidence="ECO:0000255" FT TOPO_DOM 47..57 FT /note="Cytoplasmic" FT /evidence="ECO:0000305" FT TRANSMEM 58..78 FT /note="Helical; Name=2" FT /evidence="ECO:0000255" FT TOPO_DOM 79..97 FT /note="Extracellular" FT /evidence="ECO:0000305" FT TRANSMEM 98..118 FT /note="Helical; Name=3" FT /evidence="ECO:0000255" FT TOPO_DOM 119..142 FT /note="Cytoplasmic" FT /evidence="ECO:0000305" FT TRANSMEM 143..163 FT /note="Helical; Name=4" FT /evidence="ECO:0000255" FT TOPO_DOM 164..204 FT /note="Extracellular" FT /evidence="ECO:0000305" FT TRANSMEM 205..225 FT /note="Helical; Name=5" FT /evidence="ECO:0000255" FT TOPO_DOM 226..238 FT /note="Cytoplasmic" FT /evidence="ECO:0000305" FT TRANSMEM 239..259 FT /note="Helical; Name=6" FT /evidence="ECO:0000255" FT TOPO_DOM 260..270 FT /note="Extracellular" FT /evidence="ECO:0000305" FT TRANSMEM 271..291 FT /note="Helical; Name=7" FT /evidence="ECO:0000255" FT TOPO_DOM 292..313 FT /note="Cytoplasmic" FT /evidence="ECO:0000305" FT CARBOHYD 5 FT /note="N-linked (GlcNAc...) asparagine" FT /evidence="ECO:0000255" FT DISULFID 97..179 FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00521" FT MUTAGEN 173 FT /note="E->A: Reduced affinity for Asprosin." FT /evidence="ECO:0000269|PubMed:31230984" FT MUTAGEN 186 FT /note="R->D: Reduced affinity for Asprosin." FT /evidence="ECO:0000269|PubMed:31230984" SQ SEQUENCE 313 AA; 35601 MW; 5A73BDE2898D6A5F CRC64; MEPANDTTVT EFILTGLSQT REVQLVLFVI FLSFYLFILP VNILIICTIR LDSHLSSPMY FLLANLAFLD IWYSSITAPK MLVDFFVERK IISFGGCIAQ LFFLHFVGAS EMFLLTVMAF DRYAAICRPL HYATIMNRRL CCILVALSWT GGFVHSIIQV ALIVRLPFCG PNELDNYFCD ITQVVRIACA NTFLEEMVMI FSSGLISVVC FIALLMSYAF LLTMLKKHSS SGESTSRAIS TCYSHITIVV LMFGPSIYIY ARPFDSFSLD KVVSVFHTVI FPLLNPIIYT LRNKEVKAAM RKLVNRYIFC KEK //