ID MESD_DROME Reviewed; 180 AA. AC Q8T9B6; A1Z712; DT 22-SEP-2009, integrated into UniProtKB/Swiss-Prot. DT 22-SEP-2009, sequence version 2. DT 24-NOV-2009, entry version 34. DE RecName: Full=LDLR chaperone boca; DE Flags: Precursor; GN Name=boca; ORFNames=CG30498; OS Drosophila melanogaster (Fruit fly). OC Eukaryota; Metazoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; OC Ephydroidea; Drosophilidae; Drosophila; Sophophora. OX NCBI_TaxID=7227; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=Berkeley; RX MEDLINE=20196006; PubMed=10731132; DOI=10.1126/science.287.5461.2185; RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., RA Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., RA Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., RA Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., RA Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., RA Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., RA Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., RA Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., RA Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., RA de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., RA Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., RA Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., RA Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., RA Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., RA Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., RA Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., RA Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., RA Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., RA Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., RA Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., RA Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., RA Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., RA Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., RA Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., RA Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., RA Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., RA Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., RA Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., RA Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., RA Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., RA Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.; RT "The genome sequence of Drosophila melanogaster."; RL Science 287:2185-2195(2000). RN [2] RP GENOME REANNOTATION. RX MEDLINE=22426069; PubMed=12537572; RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A., RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., RA Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., RA Lewis S.E.; RT "Annotation of the Drosophila melanogaster euchromatic genome: a RT systematic review."; RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002). RN [3] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RC STRAIN=Berkeley; TISSUE=Embryo; RA Stapleton M., Brokstein P., Hong L., Agbayani A., Carlson J.W., RA Champe M., Chavez C., Dorsett V., Dresnek D., Farfan D., Frise E., RA George R.A., Gonzalez M., Guarin H., Kronmiller B., Li P.W., Liao G., RA Miranda A., Mungall C.J., Nunoo J., Pacleb J.M., Paragas V., Park S., RA Patel S., Phouanenavong S., Wan K.H., Yu C., Lewis S.E., Rubin G.M., RA Celniker S.E.; RL Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases. RN [4] RP FUNCTION, INTERACTION WITH ARROW AND YOLKLESS, SUBCELLULAR LOCATION, RP AND MUTAGENESIS OF TRP-49. RX PubMed=12581524; DOI=10.1016/S0092-8674(02)01279-5; RA Culi J., Mann R.S.; RT "Boca, an endoplasmic reticulum protein required for wingless RT signaling and trafficking of LDL receptor family members in RT Drosophila."; RL Cell 112:343-354(2003). CC -!- FUNCTION: Chaperone specifically assisting the folding of beta- CC propeller/EGF modules within the family of low-density lipoprotein CC receptors (LDLRs). Acts as a modulator of the Wg pathway, since CC some LDLRs are coreceptors for the canonical Wnt pathway. CC -!- SUBUNIT: Monomer (By similarity). Interacts with Arrow and CC Yolkless. CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum. CC -!- DOMAIN: The LDLR maturation activity resides in the N- and C- CC terminal unstructured regions (By similarity). CC -!- SIMILARITY: Belongs to the MESD family. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AE013599; AAF59229.2; -; Genomic_DNA. DR EMBL; AY069840; AAL39985.1; -; mRNA. DR EMBL; BT044294; ACH92359.1; -; mRNA. DR RefSeq; NP_724578.1; -. DR STRING; Q8T9B6; -. DR PRIDE; Q8T9B6; -. DR Ensembl; FBtr0088953; FBpp0088027; FBgn0004132; Drosophila melanogaster. DR GeneID; 48986; -. DR FlyBase; FBgn0004132; boca. DR HOGENOM; Q8T9B6; -. DR OMA; SMKAKAK; -. DR ArrayExpress; Q8T9B6; -. DR GO; GO:0045177; C:apical part of cell; IDA:FlyBase. DR GO; GO:0005788; C:endoplasmic reticulum lumen; IDA:FlyBase. DR GO; GO:0006888; P:ER to Golgi vesicle-mediated transport; IEP:FlyBase. DR GO; GO:0032799; P:low-density lipoprotein receptor metabolic ...; IMP:FlyBase. DR GO; GO:0048477; P:oogenesis; IMP:FlyBase. DR GO; GO:0048728; P:proboscis development; IMP:FlyBase. DR GO; GO:0006612; P:protein targeting to membrane; IMP:FlyBase. DR GO; GO:0016055; P:Wnt receptor signaling pathway; IGI:FlyBase. DR InterPro; IPR019330; Mesoderm_cand-2. DR Pfam; PF10185; Mesd; 1. DR PROSITE; PS00014; ER_TARGET; 1. PE 1: Evidence at protein level; KW Chaperone; Endoplasmic reticulum; Signal; Wnt signaling pathway. FT SIGNAL 1 18 Potential. FT CHAIN 19 180 LDLR chaperone boca. FT /FTId=PRO_0000385021. FT REGION 93 166 Structured core (By similarity). FT MOTIF 177 180 Prevents secretion from ER. FT MUTAGEN 49 49 W->R: In boca1; induces lethality. FT CONFLICT 15 15 L -> V (in Ref. 3; AAL39985). SQ SEQUENCE 180 AA; 21002 MW; B51E057C901A6546 CRC64; MQTRLVLLLL ALTPLVLAKK FKEEEKPAWA KKDIRDYSEA DLERLLDQWE EDEEPLEDDE LPEHLRPQPK LDLSNLDSKS PEDLLKVSKK GRTLMTFVSV TGNPTREESD TITKLWQTSL WNNHIQAERY MVDDNRAIFL FKDGTQAWDA KDFLIEQERC KGVTIENKEY PGVNAKKDEL //