ID MESD_DROME Reviewed; 180 AA. AC Q8T9B6; A1Z712; DT 22-SEP-2009, integrated into UniProtKB/Swiss-Prot. DT 22-SEP-2009, sequence version 2. DT 02-JUN-2021, entry version 107. DE RecName: Full=LDLR chaperone boca; DE Flags: Precursor; GN Name=boca; ORFNames=CG30498; OS Drosophila melanogaster (Fruit fly). OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; OC Drosophilidae; Drosophila; Sophophora. OX NCBI_TaxID=7227; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=Berkeley; RX PubMed=10731132; DOI=10.1126/science.287.5461.2185; RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C., RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., RA Venter J.C.; RT "The genome sequence of Drosophila melanogaster."; RL Science 287:2185-2195(2000). RN [2] RP GENOME REANNOTATION. RC STRAIN=Berkeley; RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083; RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A., RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.; RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic RT review."; RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002). RN [3] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RC STRAIN=Berkeley; TISSUE=Embryo; RA Stapleton M., Brokstein P., Hong L., Agbayani A., Carlson J.W., Champe M., RA Chavez C., Dorsett V., Dresnek D., Farfan D., Frise E., George R.A., RA Gonzalez M., Guarin H., Kronmiller B., Li P.W., Liao G., Miranda A., RA Mungall C.J., Nunoo J., Pacleb J.M., Paragas V., Park S., Patel S., RA Phouanenavong S., Wan K.H., Yu C., Lewis S.E., Rubin G.M., Celniker S.E.; RL Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases. RN [4] RP FUNCTION, INTERACTION WITH ARROW AND YOLKLESS, SUBCELLULAR LOCATION, AND RP MUTAGENESIS OF TRP-49. RX PubMed=12581524; DOI=10.1016/s0092-8674(02)01279-5; RA Culi J., Mann R.S.; RT "Boca, an endoplasmic reticulum protein required for wingless signaling and RT trafficking of LDL receptor family members in Drosophila."; RL Cell 112:343-354(2003). CC -!- FUNCTION: Chaperone specifically assisting the folding of beta- CC propeller/EGF modules within the family of low-density lipoprotein CC receptors (LDLRs). Acts as a modulator of the Wg pathway, since some CC LDLRs are coreceptors for the canonical Wnt pathway. CC {ECO:0000269|PubMed:12581524}. CC -!- SUBUNIT: Monomer (By similarity). Interacts with Arrow and Yolkless. CC {ECO:0000250, ECO:0000269|PubMed:12581524}. CC -!- INTERACTION: CC Q8T9B6; Q8T9B6: boca; NbExp=3; IntAct=EBI-15914860, EBI-15914860; CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum {ECO:0000255|PROSITE- CC ProRule:PRU10138, ECO:0000269|PubMed:12581524}. CC -!- DOMAIN: The LDLR maturation activity resides in the N- and C-terminal CC unstructured regions. {ECO:0000250}. CC -!- SIMILARITY: Belongs to the MESD family. {ECO:0000305}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AE013599; AAF59229.2; -; Genomic_DNA. DR EMBL; AY069840; AAL39985.1; -; mRNA. DR EMBL; BT044294; ACH92359.1; -; mRNA. DR RefSeq; NP_724578.1; NM_165541.2. DR PDB; 3OFE; X-ray; 2.29 A; A/B=88-172. DR PDB; 3OFF; X-ray; 2.00 A; A=89-172. DR PDBsum; 3OFE; -. DR PDBsum; 3OFF; -. DR SMR; Q8T9B6; -. DR BioGRID; 71878; 76. DR DIP; DIP-59112N; -. DR ELM; Q8T9B6; -. DR IntAct; Q8T9B6; 3. DR STRING; 7227.FBpp0088027; -. DR PaxDb; Q8T9B6; -. DR PRIDE; Q8T9B6; -. DR DNASU; 48986; -. DR EnsemblMetazoa; FBtr0088953; FBpp0088027; FBgn0004132. DR GeneID; 48986; -. DR KEGG; dme:Dmel_CG30498; -. DR UCSC; CG30498-RA; d. melanogaster. DR CTD; 48986; -. DR FlyBase; FBgn0004132; boca. DR eggNOG; KOG4357; Eukaryota. DR GeneTree; ENSGT00390000000993; -. DR HOGENOM; CLU_111621_1_0_1; -. DR InParanoid; Q8T9B6; -. DR OMA; DTITKLW; -. DR OrthoDB; 1590303at2759; -. DR PhylomeDB; Q8T9B6; -. DR BioGRID-ORCS; 48986; 0 hits in 1 CRISPR screen. DR EvolutionaryTrace; Q8T9B6; -. DR GenomeRNAi; 48986; -. DR PRO; PR:Q8T9B6; -. DR Proteomes; UP000000803; Chromosome 2R. DR Bgee; FBgn0004132; Expressed in eye disc (Drosophila) and 34 other tissues. DR Genevisible; Q8T9B6; DM. DR GO; GO:0045177; C:apical part of cell; IDA:FlyBase. DR GO; GO:0005737; C:cytoplasm; IDA:FlyBase. DR GO; GO:0012505; C:endomembrane system; HDA:FlyBase. DR GO; GO:0005783; C:endoplasmic reticulum; IDA:FlyBase. DR GO; GO:0005788; C:endoplasmic reticulum lumen; IDA:FlyBase. DR GO; GO:0042802; F:identical protein binding; IPI:IntAct. DR GO; GO:0006888; P:endoplasmic reticulum to Golgi vesicle-mediated transport; IEP:FlyBase. DR GO; GO:0006457; P:protein folding; IMP:FlyBase. DR GO; GO:0006612; P:protein targeting to membrane; IMP:FlyBase. DR GO; GO:0016055; P:Wnt signaling pathway; IEA:UniProtKB-KW. DR DisProt; DP02312; -. DR InterPro; IPR019330; MESD. DR PANTHER; PTHR17600; PTHR17600; 1. DR Pfam; PF10185; Mesd; 1. DR PROSITE; PS00014; ER_TARGET; 1. PE 1: Evidence at protein level; KW 3D-structure; Chaperone; Endoplasmic reticulum; Reference proteome; Signal; KW Wnt signaling pathway. FT SIGNAL 1..18 FT /evidence="ECO:0000255" FT CHAIN 19..180 FT /note="LDLR chaperone boca" FT /id="PRO_0000385021" FT REGION 48..78 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT REGION 93..166 FT /note="Structured core" FT /evidence="ECO:0000250" FT MOTIF 177..180 FT /note="Prevents secretion from ER" FT MUTAGEN 49 FT /note="W->R: In boca1; induces lethality." FT /evidence="ECO:0000269|PubMed:12581524" FT CONFLICT 15 FT /note="L -> V (in Ref. 3; AAL39985)" FT /evidence="ECO:0000305" FT STRAND 94..103 FT /evidence="ECO:0007829|PDB:3OFF" FT HELIX 106..122 FT /evidence="ECO:0007829|PDB:3OFF" FT STRAND 127..133 FT /evidence="ECO:0007829|PDB:3OFF" FT STRAND 136..143 FT /evidence="ECO:0007829|PDB:3OFF" FT HELIX 144..146 FT /evidence="ECO:0007829|PDB:3OFF" FT HELIX 147..154 FT /evidence="ECO:0007829|PDB:3OFF" FT STRAND 160..165 FT /evidence="ECO:0007829|PDB:3OFF" FT STRAND 168..171 FT /evidence="ECO:0007829|PDB:3OFF" SQ SEQUENCE 180 AA; 21002 MW; B51E057C901A6546 CRC64; MQTRLVLLLL ALTPLVLAKK FKEEEKPAWA KKDIRDYSEA DLERLLDQWE EDEEPLEDDE LPEHLRPQPK LDLSNLDSKS PEDLLKVSKK GRTLMTFVSV TGNPTREESD TITKLWQTSL WNNHIQAERY MVDDNRAIFL FKDGTQAWDA KDFLIEQERC KGVTIENKEY PGVNAKKDEL //