ID MESD_DROME Reviewed; 180 AA. AC Q8T9B6; A1Z712; DT 22-SEP-2009, integrated into UniProtKB/Swiss-Prot. DT 22-SEP-2009, sequence version 2. DT 13-NOV-2019, entry version 100. DE RecName: Full=LDLR chaperone boca; DE Flags: Precursor; GN Name=boca; ORFNames=CG30498; OS Drosophila melanogaster (Fruit fly). OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; OC Pterygota; Neoptera; Holometabola; Diptera; Brachycera; Muscomorpha; OC Ephydroidea; Drosophilidae; Drosophila; Sophophora. OX NCBI_TaxID=7227; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=Berkeley; RX PubMed=10731132; DOI=10.1126/science.287.5461.2185; RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., RA Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., RA Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., RA Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., RA Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., RA Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., RA Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., RA Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., RA Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., RA de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., RA Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., RA Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., RA Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., RA Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., RA Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., RA Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., RA Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., RA Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., RA Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., RA Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., RA Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., RA Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., RA Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., RA Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., RA Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., RA Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., RA Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., RA Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., RA Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., RA Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., RA Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.; RT "The genome sequence of Drosophila melanogaster."; RL Science 287:2185-2195(2000). RN [2] RP GENOME REANNOTATION. RC STRAIN=Berkeley; RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083; RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A., RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., RA Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., RA Lewis S.E.; RT "Annotation of the Drosophila melanogaster euchromatic genome: a RT systematic review."; RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002). RN [3] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RC STRAIN=Berkeley; TISSUE=Embryo; RA Stapleton M., Brokstein P., Hong L., Agbayani A., Carlson J.W., RA Champe M., Chavez C., Dorsett V., Dresnek D., Farfan D., Frise E., RA George R.A., Gonzalez M., Guarin H., Kronmiller B., Li P.W., Liao G., RA Miranda A., Mungall C.J., Nunoo J., Pacleb J.M., Paragas V., Park S., RA Patel S., Phouanenavong S., Wan K.H., Yu C., Lewis S.E., Rubin G.M., RA Celniker S.E.; RL Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases. RN [4] RP FUNCTION, INTERACTION WITH ARROW AND YOLKLESS, SUBCELLULAR LOCATION, RP AND MUTAGENESIS OF TRP-49. RX PubMed=12581524; DOI=10.1016/s0092-8674(02)01279-5; RA Culi J., Mann R.S.; RT "Boca, an endoplasmic reticulum protein required for wingless RT signaling and trafficking of LDL receptor family members in RT Drosophila."; RL Cell 112:343-354(2003). CC -!- FUNCTION: Chaperone specifically assisting the folding of beta- CC propeller/EGF modules within the family of low-density lipoprotein CC receptors (LDLRs). Acts as a modulator of the Wg pathway, since CC some LDLRs are coreceptors for the canonical Wnt pathway. CC {ECO:0000269|PubMed:12581524}. CC -!- SUBUNIT: Monomer (By similarity). Interacts with Arrow and CC Yolkless. {ECO:0000250, ECO:0000269|PubMed:12581524}. CC -!- INTERACTION: CC Self; NbExp=3; IntAct=EBI-15914860, EBI-15914860; CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum {ECO:0000255|PROSITE- CC ProRule:PRU10138, ECO:0000269|PubMed:12581524}. CC -!- DOMAIN: The LDLR maturation activity resides in the N- and C- CC terminal unstructured regions. {ECO:0000250}. CC -!- SIMILARITY: Belongs to the MESD family. {ECO:0000305}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AE013599; AAF59229.2; -; Genomic_DNA. DR EMBL; AY069840; AAL39985.1; -; mRNA. DR EMBL; BT044294; ACH92359.1; -; mRNA. DR RefSeq; NP_724578.1; NM_165541.2. DR PDB; 3OFE; X-ray; 2.29 A; A/B=88-172. DR PDB; 3OFF; X-ray; 2.00 A; A=89-172. DR PDBsum; 3OFE; -. DR PDBsum; 3OFF; -. DR SMR; Q8T9B6; -. DR BioGrid; 71878; 75. DR DIP; DIP-59112N; -. DR ELM; Q8T9B6; -. DR STRING; 7227.FBpp0088027; -. DR PaxDb; Q8T9B6; -. DR PRIDE; Q8T9B6; -. DR EnsemblMetazoa; FBtr0088953; FBpp0088027; FBgn0004132. DR GeneID; 48986; -. DR KEGG; dme:Dmel_CG30498; -. DR UCSC; CG30498-RA; d. melanogaster. DR CTD; 48986; -. DR FlyBase; FBgn0004132; boca. DR eggNOG; KOG4357; Eukaryota. DR eggNOG; ENOG4111SG4; LUCA. DR GeneTree; ENSGT00390000000993; -. DR InParanoid; Q8T9B6; -. DR OMA; DTITKLW; -. DR OrthoDB; 1590303at2759; -. DR PhylomeDB; Q8T9B6; -. DR EvolutionaryTrace; Q8T9B6; -. DR GenomeRNAi; 48986; -. DR PRO; PR:Q8T9B6; -. DR Proteomes; UP000000803; Chromosome 2R. DR Bgee; FBgn0004132; Expressed in 26 organ(s), highest expression level in eye disc (Drosophila). DR Genevisible; Q8T9B6; DM. DR GO; GO:0045177; C:apical part of cell; IDA:FlyBase. DR GO; GO:0005737; C:cytoplasm; IDA:FlyBase. DR GO; GO:0012505; C:endomembrane system; HDA:FlyBase. DR GO; GO:0005783; C:endoplasmic reticulum; IDA:FlyBase. DR GO; GO:0005788; C:endoplasmic reticulum lumen; IDA:FlyBase. DR GO; GO:0042802; F:identical protein binding; IPI:IntAct. DR GO; GO:0006888; P:endoplasmic reticulum to Golgi vesicle-mediated transport; IEP:FlyBase. DR GO; GO:0006457; P:protein folding; IMP:FlyBase. DR GO; GO:0006612; P:protein targeting to membrane; IMP:FlyBase. DR GO; GO:0016055; P:Wnt signaling pathway; IEA:UniProtKB-KW. DR DisProt; DP02312; -. DR InterPro; IPR019330; MESD. DR PANTHER; PTHR17600; PTHR17600; 1. DR Pfam; PF10185; Mesd; 1. DR PROSITE; PS00014; ER_TARGET; 1. PE 1: Evidence at protein level; KW 3D-structure; Chaperone; Complete proteome; Endoplasmic reticulum; KW Reference proteome; Signal; Wnt signaling pathway. FT SIGNAL 1 18 {ECO:0000255}. FT CHAIN 19 180 LDLR chaperone boca. FT /FTId=PRO_0000385021. FT REGION 93 166 Structured core. {ECO:0000250}. FT MOTIF 177 180 Prevents secretion from ER. FT MUTAGEN 49 49 W->R: In boca1; induces lethality. FT {ECO:0000269|PubMed:12581524}. FT CONFLICT 15 15 L -> V (in Ref. 3; AAL39985). FT {ECO:0000305}. FT STRAND 94 103 {ECO:0000244|PDB:3OFF}. FT HELIX 106 122 {ECO:0000244|PDB:3OFF}. FT STRAND 127 133 {ECO:0000244|PDB:3OFF}. FT STRAND 136 143 {ECO:0000244|PDB:3OFF}. FT HELIX 144 146 {ECO:0000244|PDB:3OFF}. FT HELIX 147 154 {ECO:0000244|PDB:3OFF}. FT STRAND 160 165 {ECO:0000244|PDB:3OFF}. FT STRAND 168 171 {ECO:0000244|PDB:3OFF}. SQ SEQUENCE 180 AA; 21002 MW; B51E057C901A6546 CRC64; MQTRLVLLLL ALTPLVLAKK FKEEEKPAWA KKDIRDYSEA DLERLLDQWE EDEEPLEDDE LPEHLRPQPK LDLSNLDSKS PEDLLKVSKK GRTLMTFVSV TGNPTREESD TITKLWQTSL WNNHIQAERY MVDDNRAIFL FKDGTQAWDA KDFLIEQERC KGVTIENKEY PGVNAKKDEL //