ID NTE1_ENCCU Reviewed; 905 AA. AC Q8SVN8; DT 24-JUL-2007, integrated into UniProtKB/Swiss-Prot. DT 01-JUN-2002, sequence version 1. DT 28-JUL-2009, entry version 39. DE RecName: Full=Lysophospholipase NTE1; DE EC=3.1.1.5; DE AltName: Full=Neuropathy target esterase homolog; DE AltName: Full=Intracellular phospholipase B; GN Name=NTE1; OrderedLocusNames=ECU05_0070; OS Encephalitozoon cuniculi. OC Eukaryota; Fungi; Microsporidia; Unikaryonidae; Encephalitozoon. OX NCBI_TaxID=6035; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=GB-M1; RX MEDLINE=21576510; PubMed=11719806; DOI=10.1038/35106579; RA Katinka M.D., Duprat S., Cornillot E., Metenier G., Thomarat F., RA Prensier G., Barbe V., Peyretaillade E., Brottier P., Wincker P., RA Delbac F., El Alaoui H., Peyret P., Saurin W., Gouy M., RA Weissenbach J., Vivares C.P.; RT "Genome sequence and gene compaction of the eukaryote parasite RT Encephalitozoon cuniculi."; RL Nature 414:450-453(2001). CC -!- FUNCTION: Intracellular phospholipase B that catalyzes the double CC deacylation of phosphatidylcholine (PC) to glycerophosphocholine CC (GroPCho). Plays an important role in membrane lipid homeostasis. CC Responsible for the rapid PC turnover in response to inositol, CC elevated temperatures, or when choline is present in the growth CC medium (By similarity). CC -!- CATALYTIC ACTIVITY: 2-lysophosphatidylcholine + H(2)O = CC glycerophosphocholine + a carboxylate. CC -!- ENZYME REGULATION: Inhibited by organophosphorus esters (By CC similarity). CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Single-pass CC type I membrane protein (Potential). CC -!- SIMILARITY: Belongs to the NTE family. CC -!- SIMILARITY: Contains 2 cyclic nucleotide-binding domains. CC -!- SIMILARITY: Contains 1 patatin domain. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AL590445; CAD26524.1; -; Genomic_DNA. DR RefSeq; NP_597347.1; -. DR GeneID; 859011; -. DR GenomeReviews; AL590445_GR; ECU05_0070. DR KEGG; ecu:ECU05_0070; -. DR NMPDR; fig|6035.1.peg.494; -. DR BRENDA; 3.1.1.5; 276319. DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell. DR GO; GO:0016021; C:integral to membrane; IEA:UniProtKB-KW. DR GO; GO:0004622; F:lysophospholipase activity; IEA:EC. DR GO; GO:0016042; P:lipid catabolic process; IEA:UniProtKB-KW. DR GO; GO:0046470; P:phosphatidylcholine metabolic process; IEA:InterPro. DR InterPro; IPR000595; cNMP_bd. DR InterPro; IPR001423; Lysophospholipase_patatin_CS. DR InterPro; IPR002641; Patatin. DR InterPro; IPR014710; RmlC-like_jellyroll. DR Gene3D; G3DSA:2.60.120.10; RmlC-like_jellyroll; 2. DR Pfam; PF00027; cNMP_binding; 2. DR Pfam; PF01734; Patatin; 1. DR SMART; SM00100; cNMP; 1. DR PROSITE; PS50042; CNMP_BINDING_3; 2. DR PROSITE; PS01237; UPF0028; 1. PE 3: Inferred from homology; KW Complete proteome; Endoplasmic reticulum; Hydrolase; KW Lipid degradation; Lipid metabolism; Membrane; Repeat; Transmembrane. FT CHAIN 1 905 Lysophospholipase NTE1. FT /FTId=PRO_0000295321. FT TRANSMEM 1 21 Potential. FT TOPO_DOM 22 905 Cytoplasmic (By similarity). FT DOMAIN 610 774 Patatin. FT NP_BIND 198 319 cNMP 1. FT NP_BIND 310 428 cNMP 2. FT MOTIF 641 645 GXSXG. FT ACT_SITE 643 643 By similarity. SQ SEQUENCE 905 AA; 103088 MW; 94B9D7775EA8E92C CRC64; MVSLSLLIAI IVVTGLLAGF RHYGSSRCRT VPFKIKSRSI EKTASTRLLG MSLEDAMKRY PLFNFYPGQS IREILSSWER VTYGNGETIA APGFILQGLV EIWYREHLVC VKEKGSFLNG SMELLGYSTR GTKKARGSVE VLLIDASLVR DNIWYILFSM LRKSCIEIAC RYFELESKLV EKETMKIKDD GQGHLDLFLS FLNEKLLLNL DDGKEILRRD IEARLIGRGE TIKDSEESMD YIMYVVSGEI LVLAGESAFV FGKGSVFGYF SLFFDLYSSI KIQAREDSSV LLCSSSVLLK FGLDEKKFDH SMLLDIDESL HIIDESAEWM RLLPGDMIAQ KGDASKEIFY IGAGSVKSAS RESSSGTVIG GKECIFGESW NESSIATRIT DVVRIPSLLV DYFLERDKSF FKKYTKRLFE SGNKANGKIV SIIPVGQYKD IESFSRKLKS AIGASSLLLS RRDVVEILAR RAFDTTEEVR FMDYLTKMSR RYEIILIYVE NEYSRLLRYL LNFSDVLLAV GTTFADIPEY NVYCRIEFVK IYEERRASDE RSVKKSKKIN HGPRYLESRK ESFGQYDRVH HVLFPSKTML FCSKDFQRLA RSLLGKRIGL VLGGGGARGL AHIGVIQALE EEGIPIDCVG GTSMGAFIGA LYAKECNNYH VFKQAKRFSR KMSNIWILLL DLTYPICSMF SGHAFNRSLH SIFGDGLIQD LWLEYFCITT NIVSYEEEVH RNGMVWRYVR ASMGLCGYLP PICDNKKLLV DGGYLNNVPA DVMMGMQVEK IITVDVGTVL ENDHFDYGDT LSGFTILFNK FFGNKKFVTM EEIQYRLAYI STERKMKELE ANGNIIMLRP DLGKYRTMDF KKFDEIVACG YQSTKNAIAK WKQEGTYDVL FFSLKKQTPK RRYSV //