ID NUP93_HUMAN Reviewed; 819 AA. AC Q8N1F7; B3KPQ8; Q14705; DT 16-JAN-2004, integrated into UniProtKB/Swiss-Prot. DT 17-OCT-2006, sequence version 2. DT 26-NOV-2014, entry version 111. DE RecName: Full=Nuclear pore complex protein Nup93; DE AltName: Full=93 kDa nucleoporin; DE AltName: Full=Nucleoporin Nup93; GN Name=NUP93; Synonyms=KIAA0095; OS Homo sapiens (Human). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; OC Catarrhini; Hominidae; Homo. OX NCBI_TaxID=9606; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). RC TISSUE=Bone marrow; RX PubMed=7788527; DOI=10.1093/dnares/2.1.37; RA Nagase T., Miyajima N., Tanaka A., Sazuka T., Seki N., Sato S., RA Tabata S., Ishikawa K., Kawarabayasi Y., Kotani H., Nomura N.; RT "Prediction of the coding sequences of unidentified human genes. III. RT The coding sequences of 40 new genes (KIAA0081-KIAA0120) deduced by RT analysis of cDNA clones from human cell line KG-1."; RL DNA Res. 2:37-43(1995). RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). RC TISSUE=Amygdala, and Brain; RX PubMed=14702039; DOI=10.1038/ng1285; RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S., RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., RA Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., RA Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., RA Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., RA Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., RA Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., RA Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., RA Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., RA Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., RA Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., RA Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., RA Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., RA Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., RA Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., RA Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., RA Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., RA Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., RA Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., RA Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., RA Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.; RT "Complete sequencing and characterization of 21,243 full-length human RT cDNAs."; RL Nat. Genet. 36:40-45(2004). RN [3] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RX PubMed=15616553; DOI=10.1038/nature03187; RA Martin J., Han C., Gordon L.A., Terry A., Prabhakar S., She X., RA Xie G., Hellsten U., Chan Y.M., Altherr M., Couronne O., Aerts A., RA Bajorek E., Black S., Blumer H., Branscomb E., Brown N.C., Bruno W.J., RA Buckingham J.M., Callen D.F., Campbell C.S., Campbell M.L., RA Campbell E.W., Caoile C., Challacombe J.F., Chasteen L.A., RA Chertkov O., Chi H.C., Christensen M., Clark L.M., Cohn J.D., RA Denys M., Detter J.C., Dickson M., Dimitrijevic-Bussod M., Escobar J., RA Fawcett J.J., Flowers D., Fotopulos D., Glavina T., Gomez M., RA Gonzales E., Goodstein D., Goodwin L.A., Grady D.L., Grigoriev I., RA Groza M., Hammon N., Hawkins T., Haydu L., Hildebrand C.E., Huang W., RA Israni S., Jett J., Jewett P.B., Kadner K., Kimball H., Kobayashi A., RA Krawczyk M.-C., Leyba T., Longmire J.L., Lopez F., Lou Y., Lowry S., RA Ludeman T., Manohar C.F., Mark G.A., McMurray K.L., Meincke L.J., RA Morgan J., Moyzis R.K., Mundt M.O., Munk A.C., Nandkeshwar R.D., RA Pitluck S., Pollard M., Predki P., Parson-Quintana B., Ramirez L., RA Rash S., Retterer J., Ricke D.O., Robinson D.L., Rodriguez A., RA Salamov A., Saunders E.H., Scott D., Shough T., Stallings R.L., RA Stalvey M., Sutherland R.D., Tapia R., Tesmer J.G., Thayer N., RA Thompson L.S., Tice H., Torney D.C., Tran-Gyamfi M., Tsai M., RA Ulanovsky L.E., Ustaszewska A., Vo N., White P.S., Williams A.L., RA Wills P.L., Wu J.-R., Wu K., Yang J., DeJong P., Bruce D., RA Doggett N.A., Deaven L., Schmutz J., Grimwood J., Richardson P., RA Rokhsar D.S., Eichler E.E., Gilna P., Lucas S.M., Myers R.M., RA Rubin E.M., Pennacchio L.A.; RT "The sequence and analysis of duplication-rich human chromosome 16."; RL Nature 432:988-994(2004). RN [4] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), AND VARIANT RP ARG-509. RC TISSUE=Skin; RX PubMed=15489334; DOI=10.1101/gr.2596504; RG The MGC Project Team; RT "The status, quality, and expansion of the NIH full-length cDNA RT project: the Mammalian Gene Collection (MGC)."; RL Genome Res. 14:2121-2127(2004). RN [5] RP CHARACTERIZATION. RX PubMed=9348540; DOI=10.1091/mbc.8.10.2017; RA Grandi P., Dang T., Pane N., Shevchenko A., Mann M., Forbes D., RA Hurt E.; RT "Nup93, a vertebrate homologue of yeast Nic96p, forms a complex with a RT novel 205-kDa protein and is required for correct nuclear pore RT assembly."; RL Mol. Biol. Cell 8:2017-2038(1997). RN [6] RP ABSENCE OF INTERACTION WITH TPR, AND SUBCELLULAR LOCATION. RX PubMed=12802065; DOI=10.1091/mbc.E02-09-0620; RA Hase M.E., Cordes V.C.; RT "Direct interaction with nup153 mediates binding of Tpr to the RT periphery of the nuclear pore complex."; RL Mol. Biol. Cell 14:1923-1940(2003). RN [7] RP FUNCTION, IDENTIFICATION IN THE NUCLEAR PORE COMPLEX, AND SUBCELLULAR RP LOCATION. RX PubMed=15229283; DOI=10.1091/mbc.E04-03-0165; RA Krull S., Thyberg J., Bjorkroth B., Rackwitz H.R., Cordes V.C.; RT "Nucleoporins as components of the nuclear pore complex core structure RT and Tpr as the architectural element of the nuclear basket."; RL Mol. Biol. Cell 15:4261-4277(2004). RN [8] RP FUNCTION, INTERACTION WITH NUP53, AND IDENTIFICATION IN A COMPLEX WITH RP NUP155; NUP205 AND LAMIN B. RX PubMed=15703211; DOI=10.1091/mbc.E04-10-0857; RA Hawryluk-Gara L.A., Shibuya E.K., Wozniak R.W.; RT "Vertebrate Nup53 interacts with the nuclear lamina and is required RT for the assembly of a Nup93-containing complex."; RL Mol. Biol. Cell 16:2382-2394(2005). RN [9] RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. RC TISSUE=Cervix carcinoma; RX PubMed=18691976; DOI=10.1016/j.molcel.2008.07.007; RA Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., RA Greff Z., Keri G., Stemmann O., Mann M.; RT "Kinase-selective enrichment enables quantitative phosphoproteomics of RT the kinome across the cell cycle."; RL Mol. Cell 31:438-448(2008). RN [10] RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-52 AND SER-72, AND RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. RC TISSUE=Cervix carcinoma; RX PubMed=18669648; DOI=10.1073/pnas.0805139105; RA Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., RA Elledge S.J., Gygi S.P.; RT "A quantitative atlas of mitotic phosphorylation."; RL Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008). RN [11] RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. RC TISSUE=Leukemic T-cell; RX PubMed=19690332; DOI=10.1126/scisignal.2000007; RA Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., RA Rodionov V., Han D.K.; RT "Quantitative phosphoproteomic analysis of T cell receptor signaling RT reveals system-wide modulation of protein-protein interactions."; RL Sci. Signal. 2:RA46-RA46(2009). RN [12] RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-52 AND SER-72, AND RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. RC TISSUE=Cervix carcinoma; RX PubMed=20068231; DOI=10.1126/scisignal.2000475; RA Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., RA Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., RA Mann M.; RT "Quantitative phosphoproteomics reveals widespread full RT phosphorylation site occupancy during mitosis."; RL Sci. Signal. 3:RA3-RA3(2010). RN [13] RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. RX PubMed=21269460; DOI=10.1186/1752-0509-5-17; RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., RA Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.; RT "Initial characterization of the human central proteome."; RL BMC Syst. Biol. 5:17-17(2011). RN [14] RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-72 AND SER-75, AND RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. RX PubMed=21406692; DOI=10.1126/scisignal.2001570; RA Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., RA Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., RA Blagoev B.; RT "System-wide temporal characterization of the proteome and RT phosphoproteome of human embryonic stem cell differentiation."; RL Sci. Signal. 4:RS3-RS3(2011). CC -!- FUNCTION: Plays a role in the nuclear pore complex (NPC) assembly CC and/or maintenance. May anchor nucleoporins, but not NUP153 and CC TPR, to the NPC. {ECO:0000269|PubMed:15229283, CC ECO:0000269|PubMed:15703211}. CC -!- SUBUNIT: Part of the nuclear pore complex (NPC). Component of the CC p62 complex, a complex composed of NUP62 and NUP54. Forms a CC complex with NUP53, NUP155, NUP205 and lamin B; the interaction CC with NUP53 is direct. Does not interact with TPR. CC {ECO:0000269|PubMed:15229283, ECO:0000269|PubMed:15703211}. CC -!- SUBCELLULAR LOCATION: Nucleus membrane; Peripheral membrane CC protein. Nucleus, nuclear pore complex. Note=Localizes at the CC nuclear basket and at or near the nuclear entry to the gated CC channel of the pore. CC -!- ALTERNATIVE PRODUCTS: CC Event=Alternative splicing; Named isoforms=2; CC Name=1; CC IsoId=Q8N1F7-1; Sequence=Displayed; CC Name=2; CC IsoId=Q8N1F7-2; Sequence=VSP_043117; CC Note=No experimental confirmation available.; CC -!- SIMILARITY: Belongs to the nucleoporin interacting component (NIC) CC family. {ECO:0000305}. CC -!- SEQUENCE CAUTION: CC Sequence=BAA07680.2; Type=Erroneous initiation; Note=Translation N-terminally shortened.; Evidence={ECO:0000305}; CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; D42085; BAA07680.2; ALT_INIT; mRNA. DR EMBL; AK294176; BAH11689.1; -; mRNA. DR EMBL; BC034346; AAH34346.1; -; mRNA. DR EMBL; AC012181; -; NOT_ANNOTATED_CDS; Genomic_DNA. DR EMBL; AC106779; -; NOT_ANNOTATED_CDS; Genomic_DNA. DR EMBL; AC127456; -; NOT_ANNOTATED_CDS; Genomic_DNA. DR EMBL; AK056637; BAG51770.1; -; mRNA. DR CCDS; CCDS10769.1; -. [Q8N1F7-1] DR CCDS; CCDS55996.1; -. [Q8N1F7-2] DR RefSeq; NP_001229724.1; NM_001242795.1. [Q8N1F7-2] DR RefSeq; NP_001229725.1; NM_001242796.1. [Q8N1F7-2] DR RefSeq; NP_055484.3; NM_014669.4. [Q8N1F7-1] DR RefSeq; XP_005256320.1; XM_005256263.1. [Q8N1F7-1] DR UniGene; Hs.276878; -. DR ProteinModelPortal; Q8N1F7; -. DR SMR; Q8N1F7; 185-814. DR BioGrid; 115041; 45. DR DIP; DIP-44020N; -. DR IntAct; Q8N1F7; 18. DR MINT; MINT-3039167; -. DR STRING; 9606.ENSP00000310668; -. DR PhosphoSite; Q8N1F7; -. DR DMDM; 116242684; -. DR MaxQB; Q8N1F7; -. DR PaxDb; Q8N1F7; -. DR PRIDE; Q8N1F7; -. DR Ensembl; ENST00000308159; ENSP00000310668; ENSG00000102900. [Q8N1F7-1] DR Ensembl; ENST00000542526; ENSP00000440235; ENSG00000102900. [Q8N1F7-2] DR Ensembl; ENST00000564887; ENSP00000458039; ENSG00000102900. [Q8N1F7-2] DR GeneID; 9688; -. DR KEGG; hsa:9688; -. DR UCSC; uc002eka.3; human. [Q8N1F7-1] DR CTD; 9688; -. DR GeneCards; GC16P056764; -. DR HGNC; HGNC:28958; NUP93. DR HPA; HPA017937; -. DR MIM; 614351; gene. DR neXtProt; NX_Q8N1F7; -. DR PharmGKB; PA134912759; -. DR eggNOG; NOG312233; -. DR GeneTree; ENSGT00390000016353; -. DR HOGENOM; HOG000007350; -. DR HOVERGEN; HBG052701; -. DR InParanoid; Q8N1F7; -. DR KO; K14309; -. DR OrthoDB; EOG73JKTW; -. DR PhylomeDB; Q8N1F7; -. DR TreeFam; TF315118; -. DR Reactome; REACT_115831; ISG15 antiviral mechanism. DR Reactome; REACT_163931; Nuclear Pore Complex (NPC) Disassembly. DR Reactome; REACT_267668; Transcriptional regulation by small RNAs. DR Reactome; REACT_6190; Rev-mediated nuclear export of HIV RNA. DR Reactome; REACT_6354; Viral Messenger RNA Synthesis. DR Reactome; REACT_6804; Regulation of Glucokinase by Glucokinase Regulatory Protein. DR Reactome; REACT_7991; Vpr-mediated nuclear import of PICs. DR Reactome; REACT_9395; Nuclear import of Rev protein. DR ChiTaRS; NUP93; human. DR GeneWiki; NUP93; -. DR GenomeRNAi; 9688; -. DR NextBio; 36387; -. DR PRO; PR:Q8N1F7; -. DR Bgee; Q8N1F7; -. DR CleanEx; HS_NUP93; -. DR ExpressionAtlas; Q8N1F7; baseline and differential. DR Genevestigator; Q8N1F7; -. DR GO; GO:0016020; C:membrane; IDA:UniProtKB. DR GO; GO:0005635; C:nuclear envelope; TAS:Reactome. DR GO; GO:0031965; C:nuclear membrane; IDA:UniProtKB. DR GO; GO:0034399; C:nuclear periphery; IDA:UniProtKB. DR GO; GO:0005643; C:nuclear pore; IDA:UniProtKB. DR GO; GO:0017056; F:structural constituent of nuclear pore; IMP:UniProtKB. DR GO; GO:0005975; P:carbohydrate metabolic process; TAS:Reactome. DR GO; GO:0019221; P:cytokine-mediated signaling pathway; TAS:Reactome. DR GO; GO:0015758; P:glucose transport; TAS:Reactome. DR GO; GO:0008645; P:hexose transport; TAS:Reactome. DR GO; GO:0000278; P:mitotic cell cycle; TAS:Reactome. DR GO; GO:0007077; P:mitotic nuclear envelope disassembly; TAS:Reactome. DR GO; GO:0051028; P:mRNA transport; IEA:UniProtKB-KW. DR GO; GO:0051292; P:nuclear pore complex assembly; IMP:UniProtKB. DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW. DR GO; GO:0010827; P:regulation of glucose transport; TAS:Reactome. DR GO; GO:0044281; P:small molecule metabolic process; TAS:Reactome. DR GO; GO:0055085; P:transmembrane transport; TAS:Reactome. DR GO; GO:0016032; P:viral process; TAS:Reactome. DR InterPro; IPR007231; Nucleoporin_int_Nup93/Nic96. DR PANTHER; PTHR11225; PTHR11225; 1. DR Pfam; PF04097; Nic96; 1. PE 1: Evidence at protein level; KW Alternative splicing; Complete proteome; Membrane; mRNA transport; KW Nuclear pore complex; Nucleus; Phosphoprotein; Polymorphism; KW Protein transport; Reference proteome; Translocation; Transport. FT CHAIN 1 819 Nuclear pore complex protein Nup93. FT /FTId=PRO_0000124782. FT MOD_RES 52 52 Phosphoserine. FT {ECO:0000269|PubMed:18669648, FT ECO:0000269|PubMed:20068231}. FT MOD_RES 72 72 Phosphoserine. FT {ECO:0000269|PubMed:18669648, FT ECO:0000269|PubMed:20068231, FT ECO:0000269|PubMed:21406692}. FT MOD_RES 75 75 Phosphoserine. FT {ECO:0000269|PubMed:21406692}. FT VAR_SEQ 1 123 Missing (in isoform 2). FT {ECO:0000303|PubMed:14702039}. FT /FTId=VSP_043117. FT VARIANT 509 509 S -> R (in dbSNP:rs17853288). FT {ECO:0000269|PubMed:15489334}. FT /FTId=VAR_028160. SQ SEQUENCE 819 AA; 93488 MW; 7A611FABE964FE98 CRC64; MDTEGFGELL QQAEQLAAET EGISELPHVE RNLQEIQQAG ERLRSRTLTR TSQETADVKA SVLLGSRGLD ISHISQRLES LSAATTFEPL EPVKDTDIQG FLKNEKDNAL LSAIEESRKR TFGMAEEYHR ESMLVEWEQV KQRILHTLLA SGEDALDFTQ ESEPSYISDV GPPGRSSLDN IEMAYARQIY IYNEKIVNGH LQPNLVDLCA SVAELDDKSI SDMWTMVKQM TDVLLTPATD ALKNRSSVEV RMEFVRQALA YLEQSYKNYT LVTVFGNLHQ AQLGGVPGTY QLVRSFLNIK LPAPLPGLQD GEVEGHPVWA LIYYCMRCGD LLAASQVVNR AQHQLGEFKT WFQEYMNSKD RRLSPATENK LRLHYRRALR NNTDPYKRAV YCIIGRCDVT DNQSEVADKT EDYLWLKLNQ VCFDDDGTSS PQDRLTLSQF QKQLLEDYGE SHFTVNQQPF LYFQVLFLTA QFEAAVAFLF RMERLRCHAV HVALVLFELK LLLKSSGQSA QLLSHEPGDP PCLRRLNFVR LLMLYTRKFE STDPREALQY FYFLRDEKDS QGENMFLRCV SELVIESREF DMILGKLEND GSRKPGVIDK FTSDTKPIIN KVASVAENKG LFEEAAKLYD LAKNADKVLE LMNKLLSPVV PQISAPQSNK ERLKNMALSI AERYRAQGIS ANKFVDSTFY LLLDLITFFD EYHSGHIDRA FDIIERLKLV PLNQESVEER VAAFRNFSDE IRHNLSEVLL ATMNILFTQF KRLKGTSPSS SSRPQRVIED RDSQLRSQAR TLITFAGMIP YRTSGDTNAR LVQMEVLMN //