ID GHRL_ANGJA STANDARD; PRT; 111 AA. AC Q8JFY4; DT 21-JUN-2005, integrated into UniProtKB/Swiss-Prot. DT 01-OCT-2002, sequence version 1. DT 16-MAY-2006, entry version 16. DE Ghrelin precursor (Ghrelin-21). OS Anguilla japonica (Japanese eel). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Actinopterygii; Neopterygii; Teleostei; Anguilliformes; Anguillidae; OC Anguilla. OX NCBI_TaxID=7937; RN [1] RP NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 27-47, FUNCTION, RP TISSUE SPECIFICITY, ACYLATION AT SER-29, AND MASS SPECTROMETRY. RC TISSUE=Stomach; RX MEDLINE=22519143; PubMed=12630926; DOI=10.1677/joe.0.1760415; RA Kaiya H., Kojima M., Hosoda H., Riley L.G., Hirano T., Grau E.G., RA Kangawa K.; RT "Amidated fish ghrelin: purification, cDNA cloning in the Japanese eel RT and its biological activity."; RL J. Endocrinol. 176:415-423(2003). CC -!- FUNCTION: Specific ligand for the growth hormone secretagogue CC receptor type 1 (GHSR) inducing the release of growth hormone from CC the pituitary. Has an appetite-stimulating effect, induces CC adiposity and stimulates gastric acid secretion. Involved in CC growth regulation. CC -!- SUBCELLULAR LOCATION: Secreted protein. CC -!- TISSUE SPECIFICITY: Highest levels in stomach and anterior CC intestine. Lower levels in posterior intestine, kidney and brain. CC Low levels in heart, head kidney and middle intestine. CC -!- PTM: O-n-octanoylation or O-n-decanoylation is essential for CC activity. The O-n-decanoylated form ghrelin-21-C10 differs in the CC length of the carbon backbone of the carboxylic acid forming an CC ester bond with Ser-29. 44% of eel ghrelin is O-n-decanoylated. CC -!- MASS SPECTROMETRY: MW=2449.2; METHOD=MALDI; RANGE=27-47 (Ghrelin- CC 21-C10); NOTE=O-decanoylated form (Ref.1). CC -!- MASS SPECTROMETRY: MW=2421.7; METHOD=MALDI; RANGE=27-47 (Ghrelin- CC 21); NOTE=O-octanoylated form (Ref.1). CC -!- SIMILARITY: Belongs to the motilin family. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AB062427; BAB96565.1; -; mRNA. DR GO; GO:0005615; C:extracellular space; IC. DR GO; GO:0001664; F:G-protein-coupled receptor binding; IPI. DR GO; GO:0016608; F:growth hormone-releasing hormone activity; IDA. DR GO; GO:0007186; P:G-protein coupled receptor protein signalin...; IDA. DR GO; GO:0050791; P:regulation of physiological process; NAS. DR InterPro; IPR005441; Preproghrelin. DR PANTHER; PTHR14122; Preproghrelin; 1. DR ProDom; PD332162; Preproghrelin; 1. KW Amidation; Cleavage on pair of basic residues; KW Direct protein sequencing; Hormone; Lipoprotein; Signal. FT SIGNAL 1 26 FT PEPTIDE 27 47 Ghrelin. FT /FTId=PRO_0000019211. FT PROPEP 51 111 Removed in mature form. FT /FTId=PRO_0000019212. FT MOD_RES 47 47 Valine amide (G-48 provides amide group) FT (Probable). FT LIPID 29 29 O-decanoyl serine (in form ghrelin-21- FT C10). FT LIPID 29 29 O-octanoyl serine (in form ghrelin-21). SQ SEQUENCE 111 AA; 12831 MW; 7AF95E04DD22DE7B CRC64; MRQMKRTAYI ILLVCVLALW MDSVQAGSSF LSPSQRPQGK DKKPPRVGRR DSDGILDLFM RPPLQDEDIR HITFNTPFEI GITMTEELFQ QYGEVMQKIM QDLLMDTPAK E //