ID   ELOA2_HUMAN             Reviewed;         753 AA.
AC   Q8IYF1; Q9P2V9;
DT   11-JUL-2003, integrated into UniProtKB/Swiss-Prot.
DT   17-APR-2007, sequence version 2.
DT   07-APR-2021, entry version 162.
DE   RecName: Full=Elongin-A2;
DE            Short=EloA2;
DE   AltName: Full=RNA polymerase II transcription factor SIII subunit A2;
DE   AltName: Full=Transcription elongation factor B polypeptide 3B;
GN   Name=ELOA2 {ECO:0000312|HGNC:HGNC:30771}; Synonyms=TCEB3B, TCEB3L;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBUNIT, TISSUE SPECIFICITY, AND
RP   VARIANTS PRO-179; PHE-254; SER-403 AND THR-446.
RC   TISSUE=Testis;
RX   PubMed=10692460; DOI=10.1074/jbc.275.9.6546;
RA   Aso T., Yamazaki K., Amimoto K., Kuroiwa A., Higashi H., Matsuda Y.,
RA   Kitajima S., Hatakeyama M.;
RT   "Identification and characterization of Elongin A2, a new member of the
RT   Elongin family of transcription elongation factors, specifically expressed
RT   in the testis.";
RL   J. Biol. Chem. 275:6546-6552(2000).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Testis;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [3]
RP   VARIANT [LARGE SCALE ANALYSIS] GLN-498.
RX   PubMed=16959974; DOI=10.1126/science.1133427;
RA   Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D.,
RA   Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P.,
RA   Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V.,
RA   Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H.,
RA   Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W.,
RA   Velculescu V.E.;
RT   "The consensus coding sequences of human breast and colorectal cancers.";
RL   Science 314:268-274(2006).
CC   -!- FUNCTION: SIII, also known as elongin, is a general transcription
CC       elongation factor that increases the RNA polymerase II transcription
CC       elongation past template-encoded arresting sites. Subunit A2 is
CC       transcriptionally active but its transcription activity is not enhanced
CC       by binding to the dimeric complex of the SIII regulatory subunits B and
CC       C (elongin BC complex). {ECO:0000269|PubMed:10692460}.
CC   -!- SUBUNIT: Heterotrimer of an A (ELOA, ELOA2 or ELOA3P), ELOB and ELOC
CC       subunit. {ECO:0000269|PubMed:10692460}.
CC   -!- INTERACTION:
CC       Q8IYF1; O14647-3: CHD2; NbExp=3; IntAct=EBI-741705, EBI-11985957;
CC       Q8IYF1; O75031: HSF2BP; NbExp=5; IntAct=EBI-741705, EBI-7116203;
CC       Q8IYF1; Q6ZUT1: NKAPD1; NbExp=3; IntAct=EBI-741705, EBI-3920396;
CC       Q8IYF1; Q15287: RNPS1; NbExp=3; IntAct=EBI-741705, EBI-395959;
CC       Q8IYF1; Q8N9Q2: SREK1IP1; NbExp=3; IntAct=EBI-741705, EBI-10268630;
CC       Q8IYF1; P40337-2: VHL; NbExp=3; IntAct=EBI-741705, EBI-12157263;
CC       Q8IYF1; Q8TBK6: ZCCHC10; NbExp=3; IntAct=EBI-741705, EBI-597063;
CC       Q8IYF1; P52744: ZNF138; NbExp=3; IntAct=EBI-741705, EBI-10746567;
CC       Q8IYF1; P17027: ZNF23; NbExp=3; IntAct=EBI-741705, EBI-5657766;
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   -!- TISSUE SPECIFICITY: Specifically expressed in testis.
CC       {ECO:0000269|PubMed:10692460}.
CC   -!- DOMAIN: The BC-box, which mediates binding to the elongin BC complex,
CC       has the consensus sequence [APST]-L-x(3)-C-x(3)-[AILV].
CC       {ECO:0000250|UniProtKB:Q63187}.
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DR   EMBL; AB030834; BAA90665.1; -; mRNA.
DR   EMBL; BC036022; AAH36022.1; -; mRNA.
DR   CCDS; CCDS11932.1; -.
DR   RefSeq; NP_057511.2; NM_016427.2.
DR   SMR; Q8IYF1; -.
DR   BioGRID; 119388; 48.
DR   IntAct; Q8IYF1; 22.
DR   STRING; 9606.ENSP00000331302; -.
DR   iPTMnet; Q8IYF1; -.
DR   PhosphoSitePlus; Q8IYF1; -.
DR   BioMuta; ELOA2; -.
DR   DMDM; 145559470; -.
DR   PaxDb; Q8IYF1; -.
DR   PeptideAtlas; Q8IYF1; -.
DR   PRIDE; Q8IYF1; -.
DR   ProteomicsDB; 71165; -.
DR   Antibodypedia; 22536; 73 antibodies.
DR   DNASU; 51224; -.
DR   Ensembl; ENST00000332567; ENSP00000331302; ENSG00000206181.
DR   GeneID; 51224; -.
DR   KEGG; hsa:51224; -.
DR   UCSC; uc002lcr.2; human.
DR   CTD; 51224; -.
DR   DisGeNET; 51224; -.
DR   GeneCards; ELOA2; -.
DR   HGNC; HGNC:30771; ELOA2.
DR   HPA; ENSG00000206181; Tissue enriched (testis).
DR   MIM; 609522; gene.
DR   neXtProt; NX_Q8IYF1; -.
DR   OpenTargets; ENSG00000206181; -.
DR   PharmGKB; PA134932220; -.
DR   VEuPathDB; HostDB:ENSG00000206181.5; -.
DR   eggNOG; KOG2821; Eukaryota.
DR   GeneTree; ENSGT00390000002428; -.
DR   HOGENOM; CLU_021679_1_0_1; -.
DR   InParanoid; Q8IYF1; -.
DR   OMA; YIGVTDH; -.
DR   OrthoDB; 1174517at2759; -.
DR   PhylomeDB; Q8IYF1; -.
DR   TreeFam; TF317259; -.
DR   PathwayCommons; Q8IYF1; -.
DR   Reactome; R-HSA-112382; Formation of RNA Pol II elongation complex.
DR   Reactome; R-HSA-167152; Formation of HIV elongation complex in the absence of HIV Tat.
DR   Reactome; R-HSA-167200; Formation of HIV-1 elongation complex containing HIV-1 Tat.
DR   Reactome; R-HSA-167238; Pausing and recovery of Tat-mediated HIV elongation.
DR   Reactome; R-HSA-167243; Tat-mediated HIV elongation arrest and recovery.
DR   Reactome; R-HSA-167246; Tat-mediated elongation of the HIV-1 transcript.
DR   Reactome; R-HSA-167287; HIV elongation arrest and recovery.
DR   Reactome; R-HSA-167290; Pausing and recovery of HIV elongation.
DR   Reactome; R-HSA-674695; RNA Polymerase II Pre-transcription Events.
DR   Reactome; R-HSA-6796648; TP53 Regulates Transcription of DNA Repair Genes.
DR   Reactome; R-HSA-75955; RNA Polymerase II Transcription Elongation.
DR   BioGRID-ORCS; 51224; 7 hits in 982 CRISPR screens.
DR   GenomeRNAi; 51224; -.
DR   Pharos; Q8IYF1; Tdark.
DR   PRO; PR:Q8IYF1; -.
DR   Proteomes; UP000005640; Chromosome 18.
DR   RNAct; Q8IYF1; protein.
DR   Bgee; ENSG00000206181; Expressed in sperm and 17 other tissues.
DR   ExpressionAtlas; Q8IYF1; baseline and differential.
DR   Genevisible; Q8IYF1; HS.
DR   GO; GO:0070449; C:elongin complex; IEA:InterPro.
DR   GO; GO:0005654; C:nucleoplasm; TAS:Reactome.
DR   GO; GO:0032784; P:regulation of DNA-templated transcription, elongation; TAS:ProtInc.
DR   GO; GO:0006366; P:transcription by RNA polymerase II; TAS:Reactome.
DR   GO; GO:0006368; P:transcription elongation from RNA polymerase II promoter; TAS:Reactome.
DR   Gene3D; 1.20.930.10; -; 1.
DR   InterPro; IPR010684; RNA_pol_II_trans_fac_SIII_A.
DR   InterPro; IPR003617; TFIIS/CRSP70_N_sub.
DR   InterPro; IPR035441; TFIIS/LEDGF_dom_sf.
DR   InterPro; IPR017923; TFIIS_N.
DR   Pfam; PF06881; Elongin_A; 1.
DR   Pfam; PF08711; Med26; 1.
DR   SMART; SM00509; TFS2N; 1.
DR   SUPFAM; SSF47676; SSF47676; 1.
DR   PROSITE; PS51319; TFIIS_N; 1.
PE   1: Evidence at protein level;
KW   Nucleus; Reference proteome; Transcription; Transcription regulation.
FT   CHAIN           1..753
FT                   /note="Elongin-A2"
FT                   /id="PRO_0000086963"
FT   DOMAIN          5..80
FT                   /note="TFIIS N-terminal"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00649"
FT   REGION          500..659
FT                   /note="Activation domain"
FT                   /evidence="ECO:0000250"
FT   REGION          528..537
FT                   /note="BC-box"
FT                   /evidence="ECO:0000250|UniProtKB:Q63187"
FT   REGION          528..537
FT                   /note="Interacting with Elongin BC complex"
FT                   /evidence="ECO:0000250"
FT   VARIANT         179
FT                   /note="R -> P (in dbSNP:rs2571028)"
FT                   /evidence="ECO:0000269|PubMed:10692460"
FT                   /id="VAR_050965"
FT   VARIANT         254
FT                   /note="C -> F (in dbSNP:rs2010834)"
FT                   /evidence="ECO:0000269|PubMed:10692460"
FT                   /id="VAR_050966"
FT   VARIANT         403
FT                   /note="A -> S (in dbSNP:rs892586)"
FT                   /evidence="ECO:0000269|PubMed:10692460"
FT                   /id="VAR_050967"
FT   VARIANT         446
FT                   /note="A -> T (in dbSNP:rs3744863)"
FT                   /evidence="ECO:0000269|PubMed:10692460"
FT                   /id="VAR_061646"
FT   VARIANT         498
FT                   /note="R -> Q (in a colorectal cancer sample; somatic
FT                   mutation; dbSNP:rs138369139)"
FT                   /evidence="ECO:0000269|PubMed:16959974"
FT                   /id="VAR_035914"
FT   CONFLICT        55
FT                   /note="R -> L (in Ref. 1; BAA90665)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        236
FT                   /note="P -> L (in Ref. 1; BAA90665)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        456
FT                   /note="S -> N (in Ref. 2; AAH36022)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   753 AA;  83921 MW;  A4CFDF5A0D3AD529 CRC64;
     MAAGSTTLHA VEKLQVRLAT KTEPKKLEKY LQKLSALPMT ADILAETGIR KTVKRLRKHQ
     HVGDFARDLA ARWKKLVLVD RNTRPGPQDP EESASRQRFG EALQDQEKAW GFPENATAPR
     SPSHSPEHRR TARRTPPGQQ RPHPRSHSRE PRAERKCPRI APADSGRYRA SPTRTAPLRM
     PEGPEPAAPG KQPGRGHTHA AQGGPLLCPG CQGQPQGKAV VSHSKGHKSS RQEKRPLCAQ
     GDWHSPTLIR EKSCGACLRE ETPRMPSWAS ARDRQPSDFK TDKEGGQAGS GQRVPALEEA
     PDSHQKRPQH SHSNKKRPSL DGRDPGNGTH GLSPEEKEQL SNDRETQEGK PPTAHLDRTS
     VSSLSEVEEV DMAEEFEQPT LSCEKYLTYD QLRKQKKKTG KSATTALGDK QRKANESKGT
     RESWDSAKKL PPVQESQSER LQAAGADSAG PKTVPSHVFS ELWDLSEAWM QANYDPLSDS
     DSMTSQAKPE ALSSPKFREE AAFPGRRVNA KMPVYSGSRP ACQLQVPTLR QQCAQVLRNN
     PDALSDVGEV PYWVLEPVLE GWRPDQLYRR KKDNHALVRE TDELRRNHCF QDFKEEKPQE
     NKTWREQYLR LPDAPEQRLR VMTTNIRSAR GNNPNGREAK MICFKSVAKT PYDTSRRQEK
     SAGDADPENG EIKPASKPAG SSHTPSSQSS SGGGRDSSSS ILRWLPEKRA NPCLSSSNEH
     AAPAAKTRKQ AAKKVAPLMA KAIRDYKRRF SRR
//