ID Q8IP90_DROME Unreviewed; 810 AA. AC Q8IP90; DT 01-MAR-2003, integrated into UniProtKB/TrEMBL. DT 01-MAR-2003, sequence version 1. DT 27-MAR-2024, entry version 187. DE SubName: Full=Bruno 1, isoform B {ECO:0000313|EMBL:AAN10810.1}; DE SubName: Full=Bruno 1, isoform G {ECO:0000313|EMBL:AGB92938.1}; DE SubName: Full=FI19388p1 {ECO:0000313|EMBL:AFA28453.1}; GN Name=bru1 {ECO:0000313|EMBL:AAN10810.1, GN ECO:0000313|FlyBase:FBgn0000114}; GN Synonyms=ARET {ECO:0000313|EMBL:AAN10810.1}, Aret GN {ECO:0000313|EMBL:AAN10810.1}, aret {ECO:0000313|EMBL:AAN10810.1}, GN aret-RB {ECO:0000313|EMBL:AFA28453.1}, aret/bruno1 GN {ECO:0000313|EMBL:AAN10810.1}, arrest/Bruno GN {ECO:0000313|EMBL:AAN10810.1}, BcDNA:GM15173 GN {ECO:0000313|EMBL:AAN10810.1}, Bru {ECO:0000313|EMBL:AAN10810.1}, bru GN {ECO:0000313|EMBL:AAN10810.1}, bru-1 {ECO:0000313|EMBL:AAN10810.1}, GN Bru1 {ECO:0000313|EMBL:AAN10810.1}, Bruno GN {ECO:0000313|EMBL:AAN10810.1}, bruno {ECO:0000313|EMBL:AAN10810.1}, GN CG6319 {ECO:0000313|EMBL:AAN10810.1}, Dm Bru GN {ECO:0000313|EMBL:AAN10810.1}, Dmel\CG31762 GN {ECO:0000313|EMBL:AAN10810.1}, ms(2)01284 GN {ECO:0000313|EMBL:AAN10810.1}, NEST:bs01g04 GN {ECO:0000313|EMBL:AAN10810.1}, NEST:bs34h11 GN {ECO:0000313|EMBL:AAN10810.1}; GN ORFNames=CG31762 {ECO:0000313|EMBL:AAN10810.1, GN ECO:0000313|FlyBase:FBgn0000114}, Dmel_CG31762 GN {ECO:0000313|EMBL:AAN10810.1}; OS Drosophila melanogaster (Fruit fly). OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; OC Drosophilidae; Drosophila; Sophophora. OX NCBI_TaxID=7227 {ECO:0000313|EMBL:AAN10810.1, ECO:0000313|Proteomes:UP000000803}; RN [1] {ECO:0000313|EMBL:AAN10810.1, ECO:0000313|Proteomes:UP000000803} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=Berkeley {ECO:0000313|Proteomes:UP000000803}; RX PubMed=10731132; DOI=10.1126/science.287.5461.2185; RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C., RA Rogers Y.H., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., RA Baxter E.G., Helt G., Nelson C.R., Gabor G.L., Abril J.F., Agbayani A., RA An H.J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., RA Harris N.L., Harvey D., Heiman T.J., Hernandez J.R., Houck J., Hostin D., RA Houston K.A., Howland T.J., Wei M.H., Ibegwam C., Jalali M., Kalush F., RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., RA Kraft C., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., RA Li J., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., RA Reese M.G., Reinert K., Remington K., Saunders R.D., Scheeler F., Shen H., RA Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T., Spier E., RA Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., RA Turner R., Venter E., Wang A.H., Wang X., Wang Z.Y., Wassarman D.A., RA Weinstock G.M., Weissenbach J., Williams S.M., WoodageT, Worley K.C., RA Wu D., Yang S., Yao Q.A., Ye J., Yeh R.F., Zaveri J.S., Zhan M., Zhang G., RA Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S., RA Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.; RT "The genome sequence of Drosophila melanogaster."; RL Science 287:2185-2195(2000). RN [2] {ECO:0000313|Proteomes:UP000000803} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=Berkeley {ECO:0000313|Proteomes:UP000000803}; RX PubMed=12537568; RA Celniker S.E., Wheeler D.A., Kronmiller B., Carlson J.W., Halpern A., RA Patel S., Adams M., Champe M., Dugan S.P., Frise E., Hodgson A., RA George R.A., Hoskins R.A., Laverty T., Muzny D.M., Nelson C.R., RA Pacleb J.M., Park S., Pfeiffer B.D., Richards S., Sodergren E.J., RA Svirskas R., Tabor P.E., Wan K., Stapleton M., Sutton G.G., Venter C., RA Weinstock G., Scherer S.E., Myers E.W., Gibbs R.A., Rubin G.M.; RT "Finishing a whole-genome shotgun: release 3 of the Drosophila melanogaster RT euchromatic genome sequence."; RL Genome Biol. 3:RESEARCH0079-RESEARCH0079(2002). RN [3] {ECO:0000313|Proteomes:UP000000803} RP GENOME REANNOTATION. RC STRAIN=Berkeley {ECO:0000313|Proteomes:UP000000803}; RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083; RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A., RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.; RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic RT review."; RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002). RN [4] {ECO:0000313|Proteomes:UP000000803} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=Berkeley {ECO:0000313|Proteomes:UP000000803}; RX PubMed=12537573; RA Kaminker J.S., Bergman C.M., Kronmiller B., Carlson J., Svirskas R., RA Patel S., Frise E., Wheeler D.A., Lewis S.E., Rubin G.M., Ashburner M., RA Celniker S.E.; RT "The transposable elements of the Drosophila melanogaster euchromatin: a RT genomics perspective."; RL Genome Biol. 3:RESEARCH0084.1-RESEARCH0084.20(2002). RN [5] {ECO:0000313|EMBL:AAN10810.1, ECO:0000313|Proteomes:UP000000803} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=Berkeley {ECO:0000313|Proteomes:UP000000803}; RX PubMed=12537574; RA Hoskins R.A., Smith C.D., Carlson J.W., Carvalho A.B., Halpern A., RA Kaminker J.S., Kennedy C., Mungall C.J., Sullivan B.A., Sutton G.G., RA Yasuhara J.C., Wakimoto B.T., Myers E.W., Celniker S.E., Rubin G.M., RA Karpen G.H.; RT "Heterochromatic sequences in a Drosophila whole-genome shotgun assembly."; RL Genome Biol. 3:RESEARCH0085-RESEARCH0085(2002). RN [6] {ECO:0000313|EMBL:AAN10810.1, ECO:0000313|Proteomes:UP000000803} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=Berkeley {ECO:0000313|Proteomes:UP000000803}; RX PubMed=16110336; DOI=10.1371/journal.pcbi.0010022; RA Quesneville H., Bergman C.M., Andrieu O., Autard D., Nouaud D., RA Ashburner M., Anxolabehere D.; RT "Combined evidence annotation of transposable elements in genome RT sequences."; RL PLoS Comput. Biol. 1:166-175(2005). RN [7] {ECO:0000313|EMBL:AAN10810.1} RP NUCLEOTIDE SEQUENCE. RA Celniker S., Carlson J., Wan K., Frise E., Hoskins R., Park S., RA Svirskas R., Rubin G.; RL Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases. RN [8] {ECO:0000313|EMBL:AAN10810.1, ECO:0000313|Proteomes:UP000000803} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=Berkeley {ECO:0000313|Proteomes:UP000000803}; RX PubMed=17569856; DOI=10.1126/science.1139815; RA Smith C.D., Shu S., Mungall C.J., Karpen G.H.; RT "The Release 5.1 annotation of Drosophila melanogaster heterochromatin."; RL Science 316:1586-1591(2007). RN [9] {ECO:0000313|EMBL:AAN10810.1, ECO:0000313|Proteomes:UP000000803} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=Berkeley {ECO:0000313|Proteomes:UP000000803}; RX PubMed=17569867; DOI=10.1126/science.1139816; RA Hoskins R.A., Carlson J.W., Kennedy C., Acevedo D., Evans-Holm M., RA Frise E., Wan K.H., Park S., Mendez-Lago M., Rossi F., Villasante A., RA Dimitri P., Karpen G.H., Celniker S.E.; RT "Sequence finishing and mapping of Drosophila melanogaster RT heterochromatin."; RL Science 316:1625-1628(2007). RN [10] {ECO:0000313|EMBL:AFA28453.1} RP NUCLEOTIDE SEQUENCE. RA Carlson J., Booth B., Frise E., Park S., Wan K., Yu C., Celniker S.; RL Submitted (FEB-2012) to the EMBL/GenBank/DDBJ databases. RN [11] {ECO:0000313|EMBL:AAN10810.1} RP NUCLEOTIDE SEQUENCE. RX PubMed=26109357; DOI=.1534/g3.115.018929; RG FlyBase Consortium; RA Matthews B.B., Dos Santos G., Crosby M.A., Emmert D.B., St Pierre S.E., RA Gramates L.S., Zhou P., Schroeder A.J., Falls K., Strelets V., Russo S.M., RA Gelbart W.M., null; RT "Gene Model Annotations for Drosophila melanogaster: Impact of High- RT Throughput Data."; RL G3 (Bethesda) 5:1721-1736(2015). RN [12] {ECO:0000313|EMBL:AAN10810.1} RP NUCLEOTIDE SEQUENCE. RX PubMed=26109356; DOI=.1534/g3.115.018937; RG FlyBase Consortium; RA Crosby M.A., Gramates L.S., Dos Santos G., Matthews B.B., St Pierre S.E., RA Zhou P., Schroeder A.J., Falls K., Emmert D.B., Russo S.M., Gelbart W.M., RA null; RT "Gene Model Annotations for Drosophila melanogaster: The Rule-Benders."; RL G3 (Bethesda) 5:1737-1749(2015). RN [13] {ECO:0000313|EMBL:AAN10810.1} RP NUCLEOTIDE SEQUENCE. RX PubMed=25589440; RA Hoskins R.A., Carlson J.W., Wan K.H., Park S., Mendez I., Galle S.E., RA Booth B.W., Pfeiffer B.D., George R.A., Svirskas R., Krzywinski M., RA Schein J., Accardo M.C., Damia E., Messina G., Mendez-Lago M., RA de Pablos B., Demakova O.V., Andreyeva E.N., Boldyreva L.V., Marra M., RA Carvalho A.B., Dimitri P., Villasante A., Zhimulev I.F., Rubin G.M., RA Karpen G.H., Celniker S.E.; RT "The Release 6 reference sequence of the Drosophila melanogaster genome."; RL Genome Res. 25:445-458(2015). RN [14] {ECO:0000313|EMBL:AAN10810.1} RP NUCLEOTIDE SEQUENCE. RG Berkeley Drosophila Genome Project; RA Celniker S., Carlson J., Wan K., Pfeiffer B., Frise E., George R., RA Hoskins R., Stapleton M., Pacleb J., Park S., Svirskas R., Smith E., Yu C., RA Rubin G.; RT "Drosophila melanogaster release 4 sequence."; RL Submitted (NOV-2022) to the EMBL/GenBank/DDBJ databases. RN [15] {ECO:0000313|EMBL:AAN10810.1} RP NUCLEOTIDE SEQUENCE. RG FlyBase; RL Submitted (NOV-2022) to the EMBL/GenBank/DDBJ databases. CC -!- SIMILARITY: Belongs to the CELF/BRUNOL family. CC {ECO:0000256|ARBA:ARBA00009621}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AE014134; AAN10810.1; -; Genomic_DNA. DR EMBL; BT133212; AFA28453.1; -; mRNA. DR EMBL; AE014134; AGB92938.1; -; Genomic_DNA. DR RefSeq; NP_001260403.1; NM_001273474.1. DR RefSeq; NP_723737.1; NM_165002.2. DR SMR; Q8IP90; -. DR IntAct; Q8IP90; 1. DR STRING; 7227.FBpp0304035; -. DR PaxDb; 7227-FBpp0079926; -. DR DNASU; 34648; -. DR EnsemblMetazoa; FBtr0080344; FBpp0079926; FBgn0000114. DR EnsemblMetazoa; FBtr0331645; FBpp0304035; FBgn0000114. DR GeneID; 34648; -. DR UCSC; CG31762-RB; d. melanogaster. DR AGR; FB:FBgn0000114; -. DR CTD; 34648; -. DR FlyBase; FBgn0000114; bru1. DR VEuPathDB; VectorBase:FBgn0000114; -. DR eggNOG; KOG0144; Eukaryota. DR GeneTree; ENSGT00940000169014; -. DR OMA; YMDAFGM; -. DR OrthoDB; 406581at2759; -. DR BioGRID-ORCS; 34648; 0 hits in 3 CRISPR screens. DR GenomeRNAi; 34648; -. DR Proteomes; UP000000803; Chromosome 2L. DR Bgee; FBgn0000114; Expressed in cleaving embryo and 26 other cell types or tissues. DR ExpressionAtlas; Q8IP90; baseline and differential. DR GO; GO:0005737; C:cytoplasm; IDA:FlyBase. DR GO; GO:0016604; C:nuclear body; IDA:FlyBase. DR GO; GO:0031965; C:nuclear membrane; IDA:FlyBase. DR GO; GO:0005634; C:nucleus; IDA:FlyBase. DR GO; GO:0043186; C:P granule; IDA:FlyBase. DR GO; GO:1990904; C:ribonucleoprotein complex; IBA:GO_Central. DR GO; GO:0003730; F:mRNA 3'-UTR binding; IDA:FlyBase. DR GO; GO:0003729; F:mRNA binding; ISS:FlyBase. DR GO; GO:0003723; F:RNA binding; IDA:FlyBase. DR GO; GO:0036099; P:female germ-line stem cell population maintenance; IGI:FlyBase. DR GO; GO:0007281; P:germ cell development; IMP:FlyBase. DR GO; GO:0006376; P:mRNA splice site recognition; IBA:GO_Central. DR GO; GO:0007319; P:negative regulation of oskar mRNA translation; IDA:FlyBase. DR GO; GO:0017148; P:negative regulation of translation; IMP:FlyBase. DR GO; GO:0048477; P:oogenesis; IMP:FlyBase. DR GO; GO:0031536; P:positive regulation of exit from mitosis; IMP:FlyBase. DR GO; GO:2000738; P:positive regulation of stem cell differentiation; IMP:FlyBase. DR GO; GO:0000381; P:regulation of alternative mRNA splicing, via spliceosome; IMP:FlyBase. DR GO; GO:0014881; P:regulation of myofibril size; IMP:FlyBase. DR GO; GO:0045214; P:sarcomere organization; IMP:FlyBase. DR CDD; cd12631; RRM1_CELF1_2_Bruno; 1. DR CDD; cd12640; RRM3_Bruno_like; 1. DR Gene3D; 3.30.70.330; -; 3. DR InterPro; IPR034196; CELF1/2_RRM1. DR InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf. DR InterPro; IPR035979; RBD_domain_sf. DR InterPro; IPR000504; RRM_dom. DR PANTHER; PTHR24012:SF690; BRUNO 1, ISOFORM B-RELATED; 1. DR PANTHER; PTHR24012; RNA BINDING PROTEIN; 1. DR Pfam; PF00076; RRM_1; 3. DR SMART; SM00360; RRM; 3. DR SUPFAM; SSF54928; RNA-binding domain, RBD; 2. DR PROSITE; PS50102; RRM; 3. PE 2: Evidence at transcript level; KW Coiled coil {ECO:0000256|SAM:Coils}; KW Reference proteome {ECO:0000313|Proteomes:UP000000803}; KW RNA-binding {ECO:0000256|ARBA:ARBA00022884, ECO:0000256|PROSITE- KW ProRule:PRU00176}. FT DOMAIN 357..438 FT /note="RRM" FT /evidence="ECO:0000259|PROSITE:PS50102" FT DOMAIN 444..525 FT /note="RRM" FT /evidence="ECO:0000259|PROSITE:PS50102" FT DOMAIN 726..804 FT /note="RRM" FT /evidence="ECO:0000259|PROSITE:PS50102" FT REGION 53..72 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT REGION 95..159 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT REGION 188..219 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT REGION 238..297 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT COILED 520..547 FT /evidence="ECO:0000256|SAM:Coils" FT COMPBIAS 97..111 FT /note="Basic residues" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT COMPBIAS 125..140 FT /note="Basic residues" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT COMPBIAS 145..159 FT /note="Polar residues" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT COMPBIAS 188..217 FT /note="Basic and acidic residues" FT /evidence="ECO:0000256|SAM:MobiDB-lite" SQ SEQUENCE 810 AA; 88271 MW; F6669D8A1449D40B CRC64; MLSSLDALAG KIATATPGTG AVTADSKNTQ SLHHHHRLDY DYSAVEIEPP AEQLANSPRS ERERLQQAQQ AYEQQAQAEL EFGLAEVDAK LTQLKSSHSL QHHHSHHHQL QRHTALNQQQ QQLHHHQPHH NHHHHHPYQR PRPTSPPQQQ TSSYQQQLSQ LAALQDHHDL LQTQQELFHK QLATMQEYQR ERERERERER ERERERERER DRSSFIDNSD YDSQTEFKIC LRDSAFGMSD KSSSATNSLP NSPIHSNNNN PSLLNNNNNN SNGTSSNNSL NVGNNNSNPS LGGSNSNALV SVGSNGIMSA AGLVNNNNNP CSANRNVVAM VDDDACFRLD TDATVTYGEK EPDPDNIKMF VGQVPKSMDE SQLREMFEEY GAVHSINVLR DKATGISKGC CFVTFYTRHA ALKAQDALHN VKTLNGMYHP IQMKPADSEN RNERKLFVGM LNKKLNENDV RKLFEVHGAI EECTVLRDQN GQSKGCAFVT FATKHAAISA IKVTLSQNKI MEGCTSPLVV KFADTQKEKE QKKIQQIQAN LWNLASNINI PLGQTATSVT TPILPNPPQQ PSPVLGADAI TPASIQLLQQ LQAVGLQHQL LQALTGLGAQ QSSSATDTSA VAAAGLLTPM TVQNLAAIAA MTQPSLSNAA AAAAAATSPG SAQLTNTAAL LWSDPNPMAS AYMSTAAGLP QFGSASALST SPLASVALSA AAAAAAGKQI EGPEGCNLFI YHLPQEFTDT DLASTFLPFG NVISAKVFID KQTSLSKCFG FVSFDNPDSA QVAIKAMNGF QVGTKRLKVQ LKKPKDSKPY //