ID PYRH_STRR6 Reviewed; 247 AA. AC Q8DQ50; DT 16-AUG-2005, integrated into UniProtKB/Swiss-Prot. DT 01-MAR-2003, sequence version 1. DT 02-MAR-2010, entry version 43. DE RecName: Full=Uridylate kinase; DE Short=UK; DE EC=2.7.4.22; DE AltName: Full=Uridine monophosphate kinase; DE Short=UMP kinase; DE Short=UMPK; GN Name=pyrH; OrderedLocusNames=spr0845; OS Streptococcus pneumoniae (strain ATCC BAA-255 / R6). OC Bacteria; Firmicutes; Lactobacillales; Streptococcaceae; OC Streptococcus. OX NCBI_TaxID=171101; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RX MEDLINE=21429245; PubMed=11544234; RX DOI=10.1128/JB.183.19.5709-5717.2001; RA Hoskins J., Alborn W.E. Jr., Arnold J., Blaszczak L.C., Burgett S., RA DeHoff B.S., Estrem S.T., Fritz L., Fu D.-J., Fuller W., Geringer C., RA Gilmour R., Glass J.S., Khoja H., Kraft A.R., Lagace R.E., RA LeBlanc D.J., Lee L.N., Lefkowitz E.J., Lu J., Matsushima P., RA McAhren S.M., McHenney M., McLeaster K., Mundy C.W., Nicas T.I., RA Norris F.H., O'Gara M., Peery R.B., Robertson G.T., Rockey P., RA Sun P.-M., Winkler M.E., Yang Y., Young-Bellido M., Zhao G., RA Zook C.A., Baltz R.H., Jaskunas S.R., Rosteck P.R. Jr., Skatrud P.L., RA Glass J.I.; RT "Genome of the bacterium Streptococcus pneumoniae strain R6."; RL J. Bacteriol. 183:5709-5717(2001). CC -!- FUNCTION: Catalyzes the reversible phosphorylation of UMP to UDP CC (By similarity). CC -!- CATALYTIC ACTIVITY: ATP + UMP = ADP + UDP. CC -!- ENZYME REGULATION: Allosterically activated by GTP. Inhibited by CC UTP (By similarity). CC -!- PATHWAY: Pyrimidine metabolism; CTP biosynthesis via de novo CC pathway; UDP from UMP (UMPK route): step 1/1. CC -!- SUBUNIT: Homohexamer (By similarity). CC -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity). CC -!- SIMILARITY: Belongs to the UMP kinase family. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AE007317; AAK99649.1; -; Genomic_DNA. DR PIR; E97977; E97977. DR RefSeq; NP_358439.1; -. DR SMR; Q8DQ50; 6-241. DR STRING; Q8DQ50; -. DR GeneID; 934386; -. DR GenomeReviews; AE007317_GR; spr0845. DR KEGG; spr:spr0845; -. DR eggNOG; COG0528; -. DR HOGENOM; HBG497552; -. DR OMA; RHMEKGR; -. DR PhylomeDB; Q8DQ50; -. DR ProtClustDB; PRK00358; -. DR BioCyc; SPNE171101:SPR0845-MONOMER; -. DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell. DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW. DR GO; GO:0033862; F:UMP kinase activity; IEA:EC. DR GO; GO:0008652; P:cellular amino acid biosynthetic process; IEA:InterPro. DR GO; GO:0006221; P:pyrimidine nucleotide biosynthetic process; IEA:UniProtKB-KW. DR HAMAP; MF_01220_B; PyrH_B; 1; -. DR InterPro; IPR001048; Asp/Glu/Uridylate_kinase. DR InterPro; IPR011817; Uridylate_kinase. DR InterPro; IPR015963; Uridylate_kinase_bac. DR Gene3D; G3DSA:3.40.1160.10; Aa_kinase; 1. DR Pfam; PF00696; AA_kinase; 1. DR PIRSF; PIRSF005650; Uridylate_kin; 1. DR SUPFAM; SSF53633; Aa_kinase; 1. DR TIGRFAMs; TIGR02075; pyrH_bact; 1. PE 3: Inferred from homology; KW Allosteric enzyme; ATP-binding; Complete proteome; Cytoplasm; Kinase; KW Nucleotide-binding; Pyrimidine biosynthesis; Transferase. FT CHAIN 1 247 Uridylate kinase. FT /FTId=PRO_0000143893. FT NP_BIND 14 17 ATP (By similarity). FT NP_BIND 137 144 UMP (By similarity). FT REGION 22 27 Involved in allosteric activation by GTP FT (Potential). FT BINDING 56 56 UMP; via amide nitrogen (By similarity). FT BINDING 57 57 ATP; via amide nitrogen (By similarity). FT BINDING 61 61 ATP (By similarity). FT BINDING 76 76 UMP (By similarity). FT BINDING 165 165 ATP (By similarity). FT BINDING 171 171 ATP; via amide nitrogen and carbonyl FT oxygen (By similarity). FT BINDING 174 174 ATP (By similarity). SQ SEQUENCE 247 AA; 26693 MW; 6DEF633ED4EBFFD4 CRC64; MKMANPKYKR ILIKLSGEAL AGERGVGIDI QTVQTIAKEI QEVHSLGIEI ALVIGGGNLW RGEPAAEAGM DRVQADYTGM LGTVMNALVM ADSLQQVGVD TRVQTAIAMQ QVAEPYVRGR ALRHLEKGRI VIFGAGIGSP YFSTDTTAAL RAAEIEADAI LMAKNGVDGV YNADPKKDKT AVKFEELTHR DVINKGLRIM DSTASTLSMD NDIDLVVFNM NQSGNIKRVV FGENIGTTVS NNIEEKE //