ID I2C2_MOUSE STANDARD; PRT; 860 AA. AC Q8CJG0; Q4VAB3; Q571J6; DT 14-NOV-2003, integrated into UniProtKB/Swiss-Prot. DT 18-APR-2006, sequence version 2. DT 30-MAY-2006, entry version 23. DE Eukaryotic translation initiation factor 2C 2 (eIF2C 2) (eIF-2C 2) DE (Argonaute-2) (Slicer protein) (Piwi/argonaute family protein DE meIF2C2). GN Name=Eif2c2; Synonyms=Ago2, Kiaa4215; OS Mus musculus (Mouse). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; OC Muroidea; Muridae; Murinae; Mus. OX NCBI_TaxID=10090; RN [1] RP NUCLEOTIDE SEQUENCE [MRNA]. RX PubMed=12526743; DOI=10.1016/S0960-9822(02)01394-5; RA Doi N., Zenno S., Ueda R., Ohki-Hamazaki H., Ui-Tei K., Saigo K.; RT "Short-interfering-RNA-mediated gene silencing in mammalian cells RT requires Dicer and eIF2C translation initiation factors."; RL Curr. Biol. 13:41-46(2003). RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RC STRAIN=B5/EGFP; TISSUE=Trophoblast stem cell; RX MEDLINE=22388257; PubMed=12477932; DOI=10.1073/pnas.242603899; RA Strausberg R.L., Feingold E.A., Grouse L.H., Derge J.G., RA Klausner R.D., Collins F.S., Wagner L., Shenmen C.M., Schuler G.D., RA Altschul S.F., Zeeberg B., Buetow K.H., Schaefer C.F., Bhat N.K., RA Hopkins R.F., Jordan H., Moore T., Max S.I., Wang J., Hsieh F., RA Diatchenko L., Marusina K., Farmer A.A., Rubin G.M., Hong L., RA Stapleton M., Soares M.B., Bonaldo M.F., Casavant T.L., Scheetz T.E., RA Brownstein M.J., Usdin T.B., Toshiyuki S., Carninci P., Prange C., RA Raha S.S., Loquellano N.A., Peters G.J., Abramson R.D., Mullahy S.J., RA Bosak S.A., McEwan P.J., McKernan K.J., Malek J.A., Gunaratne P.H., RA Richards S., Worley K.C., Hale S., Garcia A.M., Gay L.J., Hulyk S.W., RA Villalon D.K., Muzny D.M., Sodergren E.J., Lu X., Gibbs R.A., RA Fahey J., Helton E., Ketteman M., Madan A., Rodrigues S., Sanchez A., RA Whiting M., Madan A., Young A.C., Shevchenko Y., Bouffard G.G., RA Blakesley R.W., Touchman J.W., Green E.D., Dickson M.C., RA Rodriguez A.C., Grimwood J., Schmutz J., Myers R.M., RA Butterfield Y.S.N., Krzywinski M.I., Skalska U., Smailus D.E., RA Schnerch A., Schein J.E., Jones S.J.M., Marra M.A.; RT "Generation and initial analysis of more than 15,000 full-length human RT and mouse cDNA sequences."; RL Proc. Natl. Acad. Sci. U.S.A. 99:16899-16903(2002). RN [3] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 158-860. RC TISSUE=Embryonic tail; RA Okazaki N., Kikuno R.F., Ohara R., Inamoto S., Nagase T., Ohara O., RA Koga H.; RT "Prediction of the coding sequences of mouse homologues of KIAA gene. RT The complete nucleotide sequences of mouse KIAA-homologous cDNAs RT identified by screening of terminal sequences of cDNA clones randomly RT sampled from size-fractionated libraries."; RL Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases. RN [4] RP FUNCTION, TISSUE SPECIFICITY, NULL MUTANT, AND MUTAGENESIS OF LEU-141; RP ASP-598; GLN-634; HIS-635; ASP-670; HIS-683; PHE-705 AND THR-745. RX PubMed=15284456; DOI=10.1126/science.1102513; RA Liu J., Carmell M.A., Rivas F.V., Marsden C.G., Thomson J.M., RA Song J.-J., Hammond S.M., Joshua-Tor L., Hannon G.J.; RT "Argonaute2 is the catalytic engine of mammalian RNAi."; RL Science 305:1437-1441(2004). CC -!- FUNCTION: Provides endonuclease activity to RNA-induced silencing CC complexes (RISC). Cleaves siRNA/mRNA heteroduplexes bound to RISC. CC Essential for embryonic development as well as RNA-mediated gene CC silencing (RNAi). CC -!- SUBUNIT: Interacts with DICER1 through its Piwi domain (By CC similarity). CC -!- INTERACTION: CC Q9UPY3:DICER1 (xeno); NbExp=2; IntAct=EBI-528299, EBI-395506; CC -!- SUBCELLULAR LOCATION: Cytoplasm; cytoplasmic bodies (By CC similarity). CC -!- TISSUE SPECIFICITY: Ubiquitous expression in 9.5 day embryos with CC highest levels in forebrain, heart, limb buds, and branchial CC arches. CC -!- DOMAIN: Piwi domain is thought to provide RNA cleavage activity CC with a mechanism similar to RNase H. CC -!- MISCELLANEOUS: Mice lacking Eif2c2 are embryonic lethal with a CC strong defect in neural tube closure and apparent cardiac failure. CC -!- SIMILARITY: Belongs to the argonaute family. CC -!- SIMILARITY: Contains 1 PAZ domain. CC -!- SIMILARITY: Contains 1 Piwi domain. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AB081472; BAC15767.1; -; mRNA. DR EMBL; BC096465; AAH96465.1; ALT_INIT; mRNA. DR EMBL; AK220193; BAD90378.1; -; mRNA. DR UniGene; Mm.150856; -. DR UniGene; Mm.274482; -. DR UniGene; Mm.392263; -. DR UniGene; Mm.86705; -. DR SMR; Q8CJG0; 223-390. DR IntAct; Q8CJG0; -. DR Ensembl; ENSMUSG00000036698; Mus musculus. DR MGI; MGI:2446632; Eif2c2. DR GO; GO:0005515; F:protein binding; IPI. DR InterPro; IPR003100; PAZ. DR InterPro; IPR003165; Piwi. DR InterPro; IPR012337; RNaseH_fold. DR Pfam; PF02170; PAZ; 1. DR Pfam; PF02171; Piwi; 1. DR PROSITE; PS50821; PAZ; 1. DR PROSITE; PS50822; PIWI; 1. KW Initiation factor; Protein biosynthesis; RNA-mediated gene silencing. FT CHAIN 1 860 Eukaryotic translation initiation factor FT 2C 2. FT /FTId=PRO_0000194058. FT DOMAIN 236 349 PAZ. FT DOMAIN 518 819 Piwi. FT MUTAGEN 141 141 L->W: No effect. FT MUTAGEN 598 598 D->A: Loss of RNA cleavage. Binds siRNA. FT MUTAGEN 634 634 Q->A: No effect. FT MUTAGEN 634 634 Q->R: Loss of RNA cleavage. Binds siRNA. FT MUTAGEN 635 635 H->P,A: Loss of RNA cleavage. Binds FT siRNA. FT MUTAGEN 670 670 D->A: Loss of RNA cleavage. Binds siRNA. FT MUTAGEN 683 683 H->Y: No effect. FT MUTAGEN 705 705 F->Y: No effect. FT MUTAGEN 745 745 T->Y: No effect. FT CONFLICT 65 65 E -> G (in Ref. 2; AAH96465). FT CONFLICT 67 67 R -> C (in Ref. 2; AAH96465). FT CONFLICT 129 129 L -> F (in Ref. 2; AAH96465). FT CONFLICT 360 360 N -> D (in Ref. 1). FT CONFLICT 563 563 N -> D (in Ref. 2; AAH96465). FT CONFLICT 711 711 R -> P (in Ref. 2; AAH96465). FT CONFLICT 761 761 S -> G (in Ref. 1). SQ SEQUENCE 860 AA; 97323 MW; 8E73A345E9E3DD9D CRC64; MYSGAGPVLA SPAPTTSPIP GYAFKPPPRP DFGTTGRTIK LQANFFEMDI PKIDIYHYEL DIKPEKRPRR VNREIVEHMV QHFKTQIFGD RKPVFDGRKN LYTAMPLPIG RDKVELEVTL PGEGKDRILK VSIKWVSCVS LQALHDALSG RLPSVPFETI QALDVVMRHL PSMRYTPVGR SFFTASEGCS NPLGGGREVW FGFHQSVRPS LWKMMLNIDV SATAFYKAQP VIEFVCEVLD FKSIEEQQKP LTDSQRVKFT KEIKGLKVEI THCGQMKRKY RVCNVTRRPA SHQTFPLQQE SGQTVECTVA QYFKDRHKLV LRYPHLPCLQ VGQEQKHTYL PLEVCNIVAG QRCIKKLTDN QTSTMIRATA RSAPDRQEEI SKLMRSASFN TDPYVREFGI MVKDEMTDVT GRVLQPPSIL YGGRNKAIAT PVQGVWDMRN KQFHTGIEIK VWAIACFAPQ RQCTEVHLKS FTEQLRKISR DAGMPIQGQP CFCKYAQGAD SVEPMFRHLK NTYAGLQLVV VILPGKTPVY AEVKRVGDTV LGMATQCVQM KNVQRTTPQT LSNLCLKINV KLGGVNNILL PQGRPPVFQQ PVIFLGADVT HPPAGDGKKP SIAAVVGSMD AHPNRYCATV RVQQHRQEII QDLAAMVREL LIQFYKSTRF KPTRIIFYRD GVSEGQFQQV LHHELLAIRE ACIKLEKDYQ PGITFIVVQK RHHTRLFCTD KNERVGKSGN IPAGTTVDTK ITHPTEFDFY LCSHAGIQGT SRPSHYHVLW DDNRFSSDEL QILTYQLCHT YVRCTRSVSI PAPAYYAHLV AFRARYHLVD KEHDSAEGSH TSGQSNGRDH QALAKAVQVH QDTLRTMYFA //