ID GSDC3_MOUSE Reviewed; 480 AA. AC Q8CB12; E9QJY6; DT 02-SEP-2008, integrated into UniProtKB/Swiss-Prot. DT 27-JUL-2011, sequence version 2. DT 17-JUN-2020, entry version 97. DE RecName: Full=Gasdermin-C3; GN Name=Gsdmc3 {ECO:0000312|MGI:MGI:3580656}; OS Mus musculus (Mouse). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; OC Murinae; Mus; Mus. OX NCBI_TaxID=10090; RN [1] {ECO:0000312|EMBL:BAC29694.1} RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RC STRAIN=C57BL/6J {ECO:0000312|EMBL:BAC29694.1}; RC TISSUE=Vagina {ECO:0000312|EMBL:BAC29694.1}; RX PubMed=16141072; DOI=10.1126/science.1112014; RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J., RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.; RT "The transcriptional landscape of the mammalian genome."; RL Science 309:1559-1563(2005). RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=C57BL/6J; RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112; RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S., RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., RA Eichler E.E., Ponting C.P.; RT "Lineage-specific biology revealed by a finished genome assembly of the RT mouse."; RL PLoS Biol. 7:E1000112-E1000112(2009). RN [3] {ECO:0000305} RP IDENTIFICATION. RX PubMed=17350798; DOI=10.1016/j.ygeno.2007.01.003; RA Tamura M., Tanaka S., Fujii T., Aoki A., Komiyama H., Ezawa K., RA Sumiyama K., Sagai T., Shiroishi T.; RT "Members of a novel gene family, Gsdm, are expressed exclusively in the RT epithelium of the skin and gastrointestinal tract in a highly tissue- RT specific manner."; RL Genomics 89:618-629(2007). CC -!- FUNCTION: The N-terminal moiety promotes pyroptosis and exhibits CC bactericidal activity. The physiological relevance of these CC observations is unknown. {ECO:0000250|UniProtKB:Q9BYG8}. CC -!- SUBUNIT: The N-terminal moiety forms homooligomer; disulfide-linked. CC May form an 16-mer complex. Oligomerization occurs in the presence of CC membranes. {ECO:0000250|UniProtKB:P57764}. CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytosol CC {ECO:0000250|UniProtKB:Q9BYG8}. Cell membrane CC {ECO:0000250|UniProtKB:P57764}. CC -!- DOMAIN: Intramolecular interactions between N- and C-terminal domains CC may be important for autoinhibition in the absence of activation CC signal. The intrinsic pyroptosis-inducing activity is carried by the N- CC terminal domain. {ECO:0000250|UniProtKB:Q5Y4Y6}. CC -!- SIMILARITY: Belongs to the gasdermin family. {ECO:0000255}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AK037079; BAC29694.1; -; mRNA. DR EMBL; AC140673; -; NOT_ANNOTATED_CDS; Genomic_DNA. DR CCDS; CCDS37088.1; -. DR RefSeq; NP_899017.2; NM_183194.3. DR RefSeq; XP_006521088.1; XM_006521025.3. DR RefSeq; XP_011243950.1; XM_011245648.2. DR RefSeq; XP_017172127.1; XM_017316638.1. DR RefSeq; XP_017172128.1; XM_017316639.1. DR RefSeq; XP_017172129.1; XM_017316640.1. DR RefSeq; XP_017172130.1; XM_017316641.1. DR RefSeq; XP_017172131.1; XM_017316642.1. DR RefSeq; XP_017172132.1; XM_017316643.1. DR RefSeq; XP_017172133.1; XM_017316644.1. DR RefSeq; XP_017172134.1; XM_017316645.1. DR RefSeq; XP_017172135.1; XM_017316646.1. DR RefSeq; XP_017172136.1; XM_017316647.1. DR RefSeq; XP_017172137.1; XM_017316648.1. DR RefSeq; XP_017172138.1; XM_017316649.1. DR RefSeq; XP_017172139.1; XM_017316650.1. DR RefSeq; XP_017172140.1; XM_017316651.1. DR RefSeq; XP_017172141.1; XM_017316652.1. DR RefSeq; XP_017172142.1; XM_017316653.1. DR SMR; Q8CB12; -. DR iPTMnet; Q8CB12; -. DR PhosphoSitePlus; Q8CB12; -. DR MaxQB; Q8CB12; -. DR PaxDb; Q8CB12; -. DR PRIDE; Q8CB12; -. DR Ensembl; ENSMUST00000089894; ENSMUSP00000087339; ENSMUSG00000055827. DR Ensembl; ENSMUST00000185526; ENSMUSP00000140272; ENSMUSG00000055827. DR Ensembl; ENSMUST00000190682; ENSMUSP00000139472; ENSMUSG00000055827. DR GeneID; 270328; -. DR KEGG; mmu:270328; -. DR UCSC; uc007vza.2; mouse. DR CTD; 270328; -. DR MGI; MGI:3580656; Gsdmc3. DR eggNOG; ENOG410IVCF; Eukaryota. DR eggNOG; ENOG4111AD5; LUCA. DR GeneTree; ENSGT00950000183140; -. DR HOGENOM; CLU_040752_2_0_1; -. DR InParanoid; Q8CB12; -. DR KO; K22143; -. DR OMA; WINDIEP; -. DR OrthoDB; 1067854at2759; -. DR TreeFam; TF331886; -. DR BioGRID-ORCS; 270328; 0 hits in 12 CRISPR screens. DR ChiTaRS; Gsdmc3; mouse. DR PRO; PR:Q8CB12; -. DR Proteomes; UP000000589; Chromosome 15. DR RNAct; Q8CB12; protein. DR Bgee; ENSMUSG00000055827; Expressed in crypt of Lieberkuhn of small intestine and 12 other tissues. DR GO; GO:0034451; C:centriolar satellite; ISO:MGI. DR GO; GO:0005737; C:cytoplasm; ISO:MGI. DR GO; GO:0005829; C:cytosol; IEA:UniProtKB-SubCell. DR GO; GO:0005739; C:mitochondrion; ISO:MGI. DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell. DR GO; GO:0005546; F:phosphatidylinositol-4,5-bisphosphate binding; IBA:GO_Central. DR GO; GO:0070273; F:phosphatidylinositol-4-phosphate binding; IBA:GO_Central. DR GO; GO:0001786; F:phosphatidylserine binding; IBA:GO_Central. DR GO; GO:0042742; P:defense response to bacterium; IBA:GO_Central. DR GO; GO:0070269; P:pyroptosis; IBA:GO_Central. DR InterPro; IPR007677; Gasdermin. DR InterPro; IPR040460; Gasdermin_pore. DR InterPro; IPR041263; Gasdermin_PUB. DR PANTHER; PTHR16399; PTHR16399; 1. DR Pfam; PF04598; Gasdermin; 1. DR Pfam; PF17708; Gasdermin_C; 1. PE 2: Evidence at transcript level; KW Cell membrane; Cytoplasm; Disulfide bond; Membrane; Necrosis; KW Reference proteome. FT CHAIN 1..480 FT /note="Gasdermin-C3" FT /id="PRO_0000347332" FT REGION 1..226 FT /note="Triggers pyroptosis" FT /evidence="ECO:0000250|UniProtKB:Q9BYG8" FT CONFLICT 383 FT /note="P -> T (in Ref. 1; BAC29694)" FT /evidence="ECO:0000305" SQ SEQUENCE 480 AA; 54188 MW; BEA95A9F8FFEEA6A CRC64; MGYSFDRASK DVVKKLQGRD LRPVECLSDA TKFRLFHILQ ETPRSGWETE DIPVGFTLLD LLEPNFPVPE PEVSAPKPFI HVQSTDLEAN LNVADIARGG VGYVGYGGYN IEVQSTSIPN PKLEILQNRK LLDKLPTFMK FCRMERKNLY VVTEAYEVSK DTMLTGLSSV NLLVKGFFKQ LFKVRGKAGR SEKYSIPIPK GSVLAYKKQQ LVIENNTCVI LPSATKKKMT FPGTPKYASA SEPTEIYRTE LQGLWINDIE PIGRIQEPAH LDFKCLQYEV SEQTRLLPEL SKDVQEVVLS SFLSMLYEGD RNVLHDLMKM LELSQLGHMD GPGGKILDEL RKDSSNPCVD LKDLILYLLQ ALMVLSDSQL NLLARSVEMR LLPHQVELVT SILQPNFKYP WNIPFTVQPQ LLAPLQGEGL AITYELLEEC GLKMELNNPR STWDLEAKMP LSALYGSLSF LQQLQKANSS FKPSLRPGYI //