ID Q8AAN6_BACTN Unreviewed; 435 AA. AC Q8AAN6; DT 01-JUN-2003, integrated into UniProtKB/TrEMBL. DT 01-JUN-2003, sequence version 1. DT 26-NOV-2014, entry version 77. DE RecName: Full=Phenylacetate-coenzyme A ligase {ECO:0000256|PIRNR:PIRNR006444}; DE EC=6.2.1.30 {ECO:0000256|PIRNR:PIRNR006444}; DE AltName: Full=Phenylacetyl-CoA ligase {ECO:0000256|PIRNR:PIRNR006444}; GN OrderedLocusNames=BT_0428 {ECO:0000313|EMBL:AAO75535.1}; OS Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC OS 10582 / E50 / VPI-5482). OC Bacteria; Bacteroidetes; Bacteroidia; Bacteroidales; Bacteroidaceae; OC Bacteroides. OX NCBI_TaxID=226186 {ECO:0000313|EMBL:AAO75535.1, ECO:0000313|Proteomes:UP000001414}; RN [1] {ECO:0000313|EMBL:AAO75535.1, ECO:0000313|Proteomes:UP000001414} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=VPI-5482 {ECO:0000313|EMBL:AAO75535.1}; RX PubMed=12663928; DOI=10.1126/science.1080029; RA Xu J., Bjursell M.K., Himrod J., Deng S., Carmichael L.K., RA Chiang H.C., Hooper L.V., Gordon J.I.; RT "A genomic view of the human-Bacteroides thetaiotaomicron symbiosis."; RL Science 299:2074-2076(2003). RN [2] {ECO:0000313|EMBL:AAO75535.1, ECO:0000313|Proteomes:UP000001414} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482 RC {ECO:0000313|Proteomes:UP000001414}; RX PubMed=19321416; DOI=10.1073/pnas.0901529106; RA Mahowald M.A., Rey F.E., Seedorf H., Turnbaugh P.J., Fulton R.S., RA Wollam A., Shah N., Wang C., Magrini V., Wilson R.K., Cantarel B.L., RA Coutinho P.M., Henrissat B., Crock L.W., Russell A., Verberkmoes N.C., RA Hettich R.L., Gordon J.I.; RT "Characterizing a model human gut microbiota composed of members of RT its two dominant bacterial phyla."; RL Proc. Natl. Acad. Sci. U.S.A. 106:5859-5864(2009). RN [3] {ECO:0000313|PDB:3QOV} RP X-RAY CRYSTALLOGRAPHY (2.20 ANGSTROMS) IN COMPLEX WITH ADP; COENZYME RP A; MAGNESIUM AND ZINC. RG Joint Center for Structural Genomics (JCSG); RT "Crystal structure of a Hypothetical Acyl-CoA ligase (BT_0428) from RT Bacteroides thetaiotaomicron VPI-5482 at 2.20 A resolution."; RL Submitted (FEB-2011) to the PDB data bank. RN [4] {ECO:0000313|PDB:3S89} RP X-RAY CRYSTALLOGRAPHY (2.41 ANGSTROMS) IN COMPLEX WITH MAGNESIUM AND RP ZINC. RG Joint center for structural genomics (JCSG); RT "Crystal structure of a Hypothetical Acyl-CoA ligase (BT_0428) from RT BACTEROIDES THETAIOTAOMICRON VPI-5482 at 2.41 A resolution."; RL Submitted (MAY-2011) to the PDB data bank. RN [5] {ECO:0000313|PDB:4R1W} RP X-RAY CRYSTALLOGRAPHY (2.41 ANGSTROMS). RG Joint Center for Structural Genomics (JCSG); RT "Crystal structure of a Hypothetical Acyl-CoA ligase (BT_0428) from RT Bacteroides thetaiotaomicron VPI-5482 at 2.41 A resolution."; RL Submitted (AUG-2014) to the PDB data bank. RN [6] {ECO:0000313|PDB:4R1L} RP X-RAY CRYSTALLOGRAPHY (2.42 ANGSTROMS) IN COMPLEX WITH ADP; AMP; RP COENZYME A AND ZINC. RG JOINT CENTER FOR STRUCTURAL GENOMICS (JCSG); RT "Crystal structure of a Hypothetical Acyl-CoA ligase (BT_0428) from RT Bacteroides thetaiotaomicron VPI-5482 at 2.42 A resolution."; RL Submitted (AUG-2014) to the PDB data bank. RN [7] {ECO:0000313|PDB:4R1M} RP X-RAY CRYSTALLOGRAPHY (2.48 ANGSTROMS) IN COMPLEX WITH AMP AND ZINC. RG JOINT CENTER FOR STRUCTURAL GENOMICS (JCSG); RT "Crystal structure of a Hypothetical Acyl-CoA ligase (BT_0428) from RT Bacteroides thetaiotaomicron VPI-5482 at 2.48 A resolution."; RL Submitted (AUG-2014) to the PDB data bank. CC -!- FUNCTION: Catalyzes the activation of phenylacetic acid (PA) to CC phenylacetyl-CoA (PA-CoA). {ECO:0000256|PIRNR:PIRNR006444}. CC -!- CATALYTIC ACTIVITY: ATP + phenylacetate + CoA = AMP + diphosphate CC + phenylacetyl-CoA. {ECO:0000256|PIRNR:PIRNR006444}. CC -!- PATHWAY: Aromatic compound metabolism; phenylacetate degradation. CC {ECO:0000256|PIRNR:PIRNR006444}. CC -!- SIMILARITY: Belongs to the phenylacetyl-CoA ligase family. CC {ECO:0000256|PIRNR:PIRNR006444}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AE015928; AAO75535.1; -; Genomic_DNA. DR RefSeq; NP_809341.1; NC_004663.1. DR PDB; 3QOV; X-ray; 2.20 A; A/B/C/D=1-435. DR PDB; 3S89; X-ray; 2.41 A; A/B/C/D=1-435. DR PDB; 4R1L; X-ray; 2.42 A; A/B/C/D=1-435. DR PDB; 4R1M; X-ray; 2.48 A; A/B/C/D=1-435. DR PDB; 4R1W; X-ray; 2.41 A; A/B/C/D=1-435. DR PDBsum; 3QOV; -. DR PDBsum; 3S89; -. DR PDBsum; 4R1L; -. DR PDBsum; 4R1M; -. DR PDBsum; 4R1W; -. DR ProteinModelPortal; Q8AAN6; -. DR STRING; 226186.BT_0428; -. DR EnsemblBacteria; AAO75535; AAO75535; BT_0428. DR GeneID; 1071713; -. DR KEGG; bth:BT_0428; -. DR PATRIC; 21055599; VBIBacThe70966_0427. DR InParanoid; Q8AAN6; -. DR KO; K01912; -. DR OMA; VECCESS; -. DR OrthoDB; EOG615VJN; -. DR BioCyc; BTHE226186:GJXV-429-MONOMER; -. DR UniPathway; UPA00930; -. DR EvolutionaryTrace; Q8AAN6; -. DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW. DR GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW. DR GO; GO:0047475; F:phenylacetate-CoA ligase activity; IEA:InterPro. DR GO; GO:0010124; P:phenylacetate catabolic process; IEA:InterPro. DR InterPro; IPR028154; AMP-dep_Lig_C. DR InterPro; IPR000873; AMP-dep_Synth/Lig. DR InterPro; IPR011880; PA_CoA_ligase. DR Pfam; PF00501; AMP-binding; 1. DR Pfam; PF14535; AMP-binding_C_2; 1. DR PIRSF; PIRSF006444; PaaK; 1. PE 1: Evidence at protein level; KW 3D-structure {ECO:0000313|PDB:3QOV, ECO:0000313|PDB:3S89}; KW Complete proteome {ECO:0000313|Proteomes:UP000001414}; KW Ligase {ECO:0000256|PIRNR:PIRNR006444, ECO:0000313|EMBL:AAO75535.1}; KW Magnesium {ECO:0000313|PDB:3QOV, ECO:0000313|PDB:3S89}; KW Metal-binding {ECO:0000313|PDB:3QOV, ECO:0000313|PDB:3S89}; KW Nucleotide-binding {ECO:0000256|PIRNR:PIRNR006444, KW ECO:0000313|PDB:3QOV}; KW Reference proteome {ECO:0000313|Proteomes:UP000001414}; KW Zinc {ECO:0000313|PDB:3QOV, ECO:0000313|PDB:3S89}. SQ SEQUENCE 435 AA; 49380 MW; 50F97E048724D7A2 CRC64; MSTQYWEEEI EIMSREKLQE LQLQRLKKTI NIAANSPYYK EVFSKNGITG DSIQSLDDIR KIPFTTKSDM RANYPFGLVA GDMKRDGVRI HSSSGTTGNP TVIVHSQHDL DSWANLVARC LYMVGIRKTD VFQNSSGYGM FTGGLGFQYG AERLGCLTVP AAAGNSKRQI KFISDFKTTA LHAIPSYAIR LAEVFQEEGI DPRETTLKTL VIGAEPHTDE QRRKIERMLN VKAYNSFGMT EMNGPGVAFE CQEQNGMHFW EDCYLVEIID PETGEPVPEG EIGELVLTTL DREMMPLIRY RTRDLTRILP GKCPCGRTHL RIDRIKGRSD DMFIIKGVNI FPMQVEKILV QFPELGSNYL ITLETVNNQD EMIVEVELSD LSTDNYIELE KIRRDIIRQL KDEILVTPKV KLVKKGSLPQ SEGKAVRVKD LRDNK //