ID   ZN227_HUMAN             Reviewed;         799 AA.
AC   Q86WZ6; B3KRU7; B7Z5P9;
DT   23-NOV-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   03-MAY-2023, entry version 166.
DE   RecName: Full=Zinc finger protein 227;
GN   Name=ZNF227;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RX   PubMed=14702039; DOI=10.1038/ng1285;
RA   Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA   Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA   Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA   Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA   Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA   Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA   Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA   Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA   Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA   Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA   Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA   Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA   Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA   Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA   Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA   Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA   Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA   Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA   Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA   Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA   Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA   Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA   Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA   Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA   Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA   Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA   Isogai T., Sugano S.;
RT   "Complete sequencing and characterization of 21,243 full-length human
RT   cDNAs.";
RL   Nat. Genet. 36:40-45(2004).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=15057824; DOI=10.1038/nature02399;
RA   Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E.,
RA   Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A.,
RA   Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S.,
RA   Carrano A.V., Caoile C., Chan Y.M., Christensen M., Cleland C.A.,
RA   Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J.,
RA   Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M.,
RA   Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W.,
RA   Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V.,
RA   Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D.,
RA   McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I.,
RA   Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L.,
RA   Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A.,
RA   She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M.,
RA   Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J.,
RA   Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E.,
RA   Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M.,
RA   Rubin E.M., Lucas S.M.;
RT   "The DNA sequence and biology of human chromosome 19.";
RL   Nature 428:529-535(2004).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA   Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA   Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA   Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA   Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA   Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA   Hunkapiller M.W., Myers E.W., Venter J.C.;
RL   Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC   TISSUE=Testis;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- FUNCTION: May be involved in transcriptional regulation.
CC   -!- INTERACTION:
CC       Q86WZ6; Q8NHQ1: CEP70; NbExp=7; IntAct=EBI-2799529, EBI-739624;
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=Q86WZ6-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q86WZ6-2; Sequence=VSP_056544;
CC   -!- SIMILARITY: Belongs to the krueppel C2H2-type zinc-finger protein
CC       family. {ECO:0000305}.
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DR   EMBL; AK092253; BAG52509.1; -; mRNA.
DR   EMBL; AK299266; BAH12985.1; -; mRNA.
DR   EMBL; AK316048; BAH14419.1; -; mRNA.
DR   EMBL; AC138470; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; CH471126; EAW57261.1; -; Genomic_DNA.
DR   EMBL; BC047570; AAH47570.1; -; mRNA.
DR   CCDS; CCDS12636.1; -. [Q86WZ6-1]
DR   CCDS; CCDS74388.1; -. [Q86WZ6-2]
DR   RefSeq; NP_001276095.1; NM_001289166.1. [Q86WZ6-1]
DR   RefSeq; NP_001276096.1; NM_001289167.1. [Q86WZ6-2]
DR   RefSeq; NP_001276097.1; NM_001289168.1. [Q86WZ6-2]
DR   RefSeq; NP_001276098.1; NM_001289169.1. [Q86WZ6-2]
DR   RefSeq; NP_001276099.1; NM_001289170.1.
DR   RefSeq; NP_872296.1; NM_182490.2. [Q86WZ6-1]
DR   RefSeq; XP_005259289.1; XM_005259232.2. [Q86WZ6-1]
DR   RefSeq; XP_011525591.1; XM_011527289.2. [Q86WZ6-1]
DR   RefSeq; XP_016882755.1; XM_017027266.1. [Q86WZ6-1]
DR   RefSeq; XP_016882757.1; XM_017027268.1. [Q86WZ6-2]
DR   RefSeq; XP_016882758.1; XM_017027269.1. [Q86WZ6-2]
DR   RefSeq; XP_016882759.1; XM_017027270.1. [Q86WZ6-2]
DR   RefSeq; XP_016882760.1; XM_017027271.1. [Q86WZ6-2]
DR   AlphaFoldDB; Q86WZ6; -.
DR   SMR; Q86WZ6; -.
DR   BioGRID; 113553; 73.
DR   IntAct; Q86WZ6; 11.
DR   MINT; Q86WZ6; -.
DR   STRING; 9606.ENSP00000482749; -.
DR   iPTMnet; Q86WZ6; -.
DR   PhosphoSitePlus; Q86WZ6; -.
DR   BioMuta; ZNF227; -.
DR   DMDM; 55976727; -.
DR   EPD; Q86WZ6; -.
DR   jPOST; Q86WZ6; -.
DR   MassIVE; Q86WZ6; -.
DR   MaxQB; Q86WZ6; -.
DR   PaxDb; Q86WZ6; -.
DR   PeptideAtlas; Q86WZ6; -.
DR   ProteomicsDB; 6708; -.
DR   ProteomicsDB; 70216; -. [Q86WZ6-1]
DR   Antibodypedia; 17695; 162 antibodies from 24 providers.
DR   DNASU; 7770; -.
DR   Ensembl; ENST00000313040.12; ENSP00000321049.6; ENSG00000131115.16. [Q86WZ6-1]
DR   Ensembl; ENST00000391961.6; ENSP00000375823.2; ENSG00000131115.16. [Q86WZ6-2]
DR   Ensembl; ENST00000589005.5; ENSP00000467577.1; ENSG00000131115.16. [Q86WZ6-2]
DR   Ensembl; ENST00000621083.4; ENSP00000482749.1; ENSG00000131115.16. [Q86WZ6-1]
DR   GeneID; 7770; -.
DR   KEGG; hsa:7770; -.
DR   MANE-Select; ENST00000313040.12; ENSP00000321049.6; NM_182490.3; NP_872296.1.
DR   UCSC; uc002oyu.5; human. [Q86WZ6-1]
DR   AGR; HGNC:13020; -.
DR   CTD; 7770; -.
DR   GeneCards; ZNF227; -.
DR   HGNC; HGNC:13020; ZNF227.
DR   HPA; ENSG00000131115; Low tissue specificity.
DR   neXtProt; NX_Q86WZ6; -.
DR   OpenTargets; ENSG00000131115; -.
DR   PharmGKB; PA37599; -.
DR   VEuPathDB; HostDB:ENSG00000131115; -.
DR   eggNOG; KOG1721; Eukaryota.
DR   GeneTree; ENSGT00940000162819; -.
DR   HOGENOM; CLU_002678_31_4_1; -.
DR   InParanoid; Q86WZ6; -.
DR   OMA; ICEDFMK; -.
DR   OrthoDB; 5249266at2759; -.
DR   PhylomeDB; Q86WZ6; -.
DR   TreeFam; TF350845; -.
DR   PathwayCommons; Q86WZ6; -.
DR   Reactome; R-HSA-212436; Generic Transcription Pathway.
DR   SignaLink; Q86WZ6; -.
DR   BioGRID-ORCS; 7770; 11 hits in 1175 CRISPR screens.
DR   ChiTaRS; ZNF227; human.
DR   GenomeRNAi; 7770; -.
DR   Pharos; Q86WZ6; Tdark.
DR   PRO; PR:Q86WZ6; -.
DR   Proteomes; UP000005640; Chromosome 19.
DR   RNAct; Q86WZ6; protein.
DR   Bgee; ENSG00000131115; Expressed in secondary oocyte and 171 other tissues.
DR   ExpressionAtlas; Q86WZ6; baseline and differential.
DR   Genevisible; Q86WZ6; HS.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; IBA:GO_Central.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR   CDD; cd07765; KRAB_A-box; 1.
DR   Gene3D; 6.10.140.140; -; 1.
DR   Gene3D; 3.30.160.60; Classic Zinc Finger; 19.
DR   InterPro; IPR001909; KRAB.
DR   InterPro; IPR036051; KRAB_dom_sf.
DR   InterPro; IPR036236; Znf_C2H2_sf.
DR   InterPro; IPR013087; Znf_C2H2_type.
DR   PANTHER; PTHR24390; ZINC FINGER PROTEIN; 1.
DR   PANTHER; PTHR24390:SF88; ZINC FINGER PROTEIN 239; 1.
DR   Pfam; PF01352; KRAB; 1.
DR   Pfam; PF00096; zf-C2H2; 16.
DR   SMART; SM00349; KRAB; 1.
DR   SMART; SM00355; ZnF_C2H2; 18.
DR   SUPFAM; SSF57667; beta-beta-alpha zinc fingers; 11.
DR   SUPFAM; SSF109640; KRAB domain (Kruppel-associated box); 1.
DR   PROSITE; PS50805; KRAB; 1.
DR   PROSITE; PS00028; ZINC_FINGER_C2H2_1; 18.
DR   PROSITE; PS50157; ZINC_FINGER_C2H2_2; 19.
PE   1: Evidence at protein level;
KW   Alternative splicing; DNA-binding; Metal-binding; Nucleus;
KW   Reference proteome; Repeat; Transcription; Transcription regulation; Zinc;
KW   Zinc-finger.
FT   CHAIN           1..799
FT                   /note="Zinc finger protein 227"
FT                   /id="PRO_0000047468"
FT   DOMAIN          23..94
FT                   /note="KRAB"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00119"
FT   ZN_FING         250..272
FT                   /note="C2H2-type 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT   ZN_FING         269..291
FT                   /note="C2H2-type 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT   ZN_FING         324..346
FT                   /note="C2H2-type 3"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT   ZN_FING         352..374
FT                   /note="C2H2-type 4"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT   ZN_FING         380..402
FT                   /note="C2H2-type 5"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT   ZN_FING         408..430
FT                   /note="C2H2-type 6"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT   ZN_FING         436..458
FT                   /note="C2H2-type 7"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT   ZN_FING         464..486
FT                   /note="C2H2-type 8"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT   ZN_FING         492..514
FT                   /note="C2H2-type 9"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT   ZN_FING         520..542
FT                   /note="C2H2-type 10"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT   ZN_FING         548..570
FT                   /note="C2H2-type 11"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT   ZN_FING         576..598
FT                   /note="C2H2-type 12"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT   ZN_FING         604..626
FT                   /note="C2H2-type 13"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT   ZN_FING         632..654
FT                   /note="C2H2-type 14"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT   ZN_FING         660..682
FT                   /note="C2H2-type 15"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT   ZN_FING         688..710
FT                   /note="C2H2-type 16"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT   ZN_FING         716..738
FT                   /note="C2H2-type 17"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT   ZN_FING         744..766
FT                   /note="C2H2-type 18"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT   ZN_FING         772..794
FT                   /note="C2H2-type 19"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT   VAR_SEQ         1..51
FT                   /note="Missing (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:14702039"
FT                   /id="VSP_056544"
SQ   SEQUENCE   799 AA;  92033 MW;  8D3506F40D71FA8A CRC64;
     MPSQNYDLPQ KKQEKMTKFQ EAVTFKDVAV VFSREELRLL DLTQRKLYRD VMVENFKNLV
     AVGHLPFQPD MVSQLEAEEK LWMMETETQR SSKHQNKMET LQKFALKYLS NQELSCWQIW
     KQVASELTRC LQGKSSQLLQ GDSIQVSENE NNIMNPKGDS SIYIENQEFP FWRTQHSCGN
     TYLSESQIQS RGKQIDVKNN LQIHEDFMKK SPFHEHIKTD TEPKPCKGNE YGKIISDGSN
     QKLPLGEKPH PCGECGRGFS YSPRLPLHPN VHTGEKCFSQ SSHLRTHQRI HPGEKLNRCH
     ESGDCFNKSS FHSYQSNHTG EKSYRCDSCG KGFSSSTGLI IHYRTHTGEK PYKCEECGKC
     FSQSSNFQCH QRVHTEEKPY KCEECGKGFG WSVNLRVHQR VHRGEKPYKC EECGKGFTQA
     AHFHIHQRVH TGEKPYKCDV CGKGFSHNSP LICHRRVHTG EKPYKCEACG KGFTRNTDLH
     IHFRVHTGEK PYKCKECGKG FSQASNLQVH QNVHTGEKRF KCETCGKGFS QSSKLQTHQR
     VHTGEKPYRC DVCGKDFSYS SNLKLHQVIH TGEKPYKCEE CGKGFSWRSN LHAHQRVHSG
     EKPYKCEQCD KSFSQAIDFR VHQRVHTGEK PYKCGVCGKG FSQSSGLQSH QRVHTGEKPY
     KCDVCGKGFR YSSQFIYHQR GHTGEKPYKC EECGKGFGRS LNLRHHQRVH TGEKPHICEE
     CGKAFSLPSN LRVHLGVHTR EKLFKCEECG KGFSQSARLE AHQRVHTGEK PYKCDICDKD
     FRHRSRLTYH QKVHTGKKL
//