ID   Q84A41_STRAG            Unreviewed;       502 AA.
AC   Q84A41;
DT   01-JUN-2003, integrated into UniProtKB/TrEMBL.
DT   01-JUN-2003, sequence version 1.
DT   19-JAN-2022, entry version 102.
DE   SubName: Full=LPXTG cell wall anchor domain-containing protein {ECO:0000313|EMBL:RRA58087.1};
DE   SubName: Full=PI-2b backbone protein {ECO:0000313|EMBL:ACM07736.1};
DE   SubName: Full=Spb1 {ECO:0000313|EMBL:AAO49409.1};
DE   SubName: Full=Surface protein Spb1 {ECO:0000313|EMBL:SUN03617.1};
GN   Name=spb1 {ECO:0000313|EMBL:AAO49409.1};
GN   ORFNames=D5F95_09540 {ECO:0000313|EMBL:RRA58087.1}, NCTC8183_00997
GN   {ECO:0000313|EMBL:SUN03617.1}, NCTC8185_01741
GN   {ECO:0000313|EMBL:SUN14457.1};
OS   Streptococcus agalactiae.
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC   Streptococcus.
OX   NCBI_TaxID=1311 {ECO:0000313|EMBL:AAO49409.1};
RN   [1] {ECO:0000313|EMBL:AAO49409.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=874391 {ECO:0000313|EMBL:AAO49409.1};
RX   PubMed=14638773; DOI=10.1128/IAI.71.12.6857-6863.2003;
RA   Adderson E.E., Takahashi S., Wang Y., Armstrong J., Miller D.V.,
RA   Bohnsack J.F.;
RT   "Subtractive hybridization identifies a novel predicted protein mediating
RT   epithelial cell invasion by virulent serotype III group B Streptococcus
RT   agalactiae.";
RL   Infect. Immun. 71:6857-6863(2003).
RN   [2] {ECO:0000313|EMBL:ACM07736.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=2177 {ECO:0000313|EMBL:ACM07764.1}, 5376
RC   {ECO:0000313|EMBL:ACM07750.1}, 5435 {ECO:0000313|EMBL:ACM07751.1},
RC   5592 {ECO:0000313|EMBL:ACM07752.1}, 5604
RC   {ECO:0000313|EMBL:ACM07753.1}, 5667 {ECO:0000313|EMBL:ACM07754.1},
RC   5883 {ECO:0000313|EMBL:ACM07756.1}, 5978
RC   {ECO:0000313|EMBL:ACM07757.1}, 5986 {ECO:0000313|EMBL:ACM07758.1},
RC   6213 {ECO:0000313|EMBL:ACM07759.1}, 6217
RC   {ECO:0000313|EMBL:ACM07760.1}, 6227 {ECO:0000313|EMBL:ACM07761.1},
RC   6236 {ECO:0000313|EMBL:ACM07762.1}, 6265
RC   {ECO:0000313|EMBL:ACM07763.1}, 6267 {ECO:0000313|EMBL:ACM07765.1},
RC   6362 {ECO:0000313|EMBL:ACM07767.1}, 6366
RC   {ECO:0000313|EMBL:ACM07768.1}, 6367 {ECO:0000313|EMBL:ACM07769.1},
RC   6373 {ECO:0000313|EMBL:ACM07770.1}, 6386
RC   {ECO:0000313|EMBL:ACM07771.1}, 6387 {ECO:0000313|EMBL:ACM07772.1},
RC   ABC020006403 {ECO:0000313|EMBL:ACM07736.1}, ABC020006988
RC   {ECO:0000313|EMBL:ACM07737.1}, ABC020015469
RC   {ECO:0000313|EMBL:ACM07744.1}, ABC020016814
RC   {ECO:0000313|EMBL:ACM07738.1}, ABC020016854
RC   {ECO:0000313|EMBL:ACM07739.1}, ABC020016915
RC   {ECO:0000313|EMBL:ACM07740.1}, ABC020017623
RC   {ECO:0000313|EMBL:ACM07741.1}, ABC020017646
RC   {ECO:0000313|EMBL:ACM07742.1}, ABC020017751
RC   {ECO:0000313|EMBL:ACM07743.1}, ABC020021168
RC   {ECO:0000313|EMBL:ACM07745.1}, ABC020023529
RC   {ECO:0000313|EMBL:ACM07746.1}, ABC020024322
RC   {ECO:0000313|EMBL:ACM07747.1}, ABC020024346
RC   {ECO:0000313|EMBL:ACM07748.1}, and ABC020027259
RC   {ECO:0000313|EMBL:ACM07749.1};
RX   PubMed=19086816; DOI=10.1086/595564;
RA   Margarit I., Rinaudo C.D., Galeotti C.L., Maione D., Ghezzo C.,
RA   Buttazzoni E., Rosini R., Runci Y., Mora M., Buccato S., Pagani M.,
RA   Tresoldi E., Berardi A., Creti R., Baker C.J., Telford J.L., Grandi G.;
RT   "Preventing bacterial infections with pilus-based vaccines: the group B
RT   streptococcus paradigm.";
RL   J. Infect. Dis. 199:108-115(2009).
RN   [3] {ECO:0000313|Proteomes:UP000254076, ECO:0000313|Proteomes:UP000254769}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NCTC8183 {ECO:0000313|EMBL:SUN03617.1,
RC   ECO:0000313|Proteomes:UP000254769}, and NCTC8185
RC   {ECO:0000313|EMBL:SUN14457.1, ECO:0000313|Proteomes:UP000254076};
RG   Pathogen Informatics;
RA   Doyle S.;
RL   Submitted (JUN-2018) to the EMBL/GenBank/DDBJ databases.
RN   [4] {ECO:0000313|EMBL:RRA58087.1, ECO:0000313|Proteomes:UP000272566}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=JF4680 {ECO:0000313|EMBL:RRA58087.1,
RC   ECO:0000313|Proteomes:UP000272566};
RA   Rothen J.;
RT   "Subspecies typing of Streptococcus agalactiae based on ribosomal subunit
RT   protein mass variation by MALDI-TOF MS.";
RL   Submitted (SEP-2018) to the EMBL/GenBank/DDBJ databases.
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DR   EMBL; AF485279; AAO49409.1; -; Genomic_DNA.
DR   EMBL; EU929085; ACM07736.1; -; Genomic_DNA.
DR   EMBL; EU929086; ACM07737.1; -; Genomic_DNA.
DR   EMBL; EU929087; ACM07738.1; -; Genomic_DNA.
DR   EMBL; EU929088; ACM07739.1; -; Genomic_DNA.
DR   EMBL; EU929089; ACM07740.1; -; Genomic_DNA.
DR   EMBL; EU929090; ACM07741.1; -; Genomic_DNA.
DR   EMBL; EU929091; ACM07742.1; -; Genomic_DNA.
DR   EMBL; EU929092; ACM07743.1; -; Genomic_DNA.
DR   EMBL; EU929093; ACM07744.1; -; Genomic_DNA.
DR   EMBL; EU929094; ACM07745.1; -; Genomic_DNA.
DR   EMBL; EU929095; ACM07746.1; -; Genomic_DNA.
DR   EMBL; EU929096; ACM07747.1; -; Genomic_DNA.
DR   EMBL; EU929097; ACM07748.1; -; Genomic_DNA.
DR   EMBL; EU929098; ACM07749.1; -; Genomic_DNA.
DR   EMBL; EU929099; ACM07750.1; -; Genomic_DNA.
DR   EMBL; EU929100; ACM07751.1; -; Genomic_DNA.
DR   EMBL; EU929101; ACM07752.1; -; Genomic_DNA.
DR   EMBL; EU929102; ACM07753.1; -; Genomic_DNA.
DR   EMBL; EU929103; ACM07754.1; -; Genomic_DNA.
DR   EMBL; EU929106; ACM07756.1; -; Genomic_DNA.
DR   EMBL; EU929107; ACM07757.1; -; Genomic_DNA.
DR   EMBL; EU929108; ACM07758.1; -; Genomic_DNA.
DR   EMBL; EU929109; ACM07759.1; -; Genomic_DNA.
DR   EMBL; EU929110; ACM07760.1; -; Genomic_DNA.
DR   EMBL; EU929111; ACM07761.1; -; Genomic_DNA.
DR   EMBL; EU929112; ACM07762.1; -; Genomic_DNA.
DR   EMBL; EU929113; ACM07763.1; -; Genomic_DNA.
DR   EMBL; EU929114; ACM07764.1; -; Genomic_DNA.
DR   EMBL; EU929115; ACM07765.1; -; Genomic_DNA.
DR   EMBL; EU929117; ACM07767.1; -; Genomic_DNA.
DR   EMBL; EU929118; ACM07768.1; -; Genomic_DNA.
DR   EMBL; EU929119; ACM07769.1; -; Genomic_DNA.
DR   EMBL; EU929120; ACM07770.1; -; Genomic_DNA.
DR   EMBL; EU929121; ACM07771.1; -; Genomic_DNA.
DR   EMBL; EU929122; ACM07772.1; -; Genomic_DNA.
DR   EMBL; QYTS01000055; RRA58087.1; -; Genomic_DNA.
DR   EMBL; UHEL01000004; SUN03617.1; -; Genomic_DNA.
DR   EMBL; UHEQ01000004; SUN14457.1; -; Genomic_DNA.
DR   RefSeq; WP_000728388.1; NZ_WHUK01000006.1.
DR   EnsemblBacteria; KLL35730; KLL35730; WA02_00535.
DR   EnsemblBacteria; KLL84331; KLL84331; WA05_05350.
DR   EnsemblBacteria; SUN03617; SUN03617; NCTC8183_00997.
DR   EnsemblBacteria; SUN28846; SUN28846; NCTC9828_01107.
DR   GeneID; 66886284; -.
DR   KEGG; sagg:EN73_07000; -.
DR   PATRIC; fig|1311.1180.peg.1520; -.
DR   OrthoDB; 275445at2; -.
DR   Proteomes; UP000254076; Unassembled WGS sequence.
DR   Proteomes; UP000254769; Unassembled WGS sequence.
DR   Proteomes; UP000272566; Unassembled WGS sequence.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   Gene3D; 2.60.40.10; -; 1.
DR   InterPro; IPR013783; Ig-like_fold.
DR   InterPro; IPR019931; LPXTG_anchor.
DR   InterPro; IPR041033; Prealbumin-like.
DR   Pfam; PF00746; Gram_pos_anchor; 1.
DR   Pfam; PF17802; SpaA; 1.
PE   4: Predicted;
KW   Cell wall {ECO:0000256|ARBA:ARBA00022512};
KW   Membrane {ECO:0000256|SAM:Phobius};
KW   Peptidoglycan-anchor {ECO:0000256|ARBA:ARBA00023088};
KW   Secreted {ECO:0000256|ARBA:ARBA00022512};
KW   Signal {ECO:0000256|ARBA:ARBA00022729, ECO:0000256|SAM:SignalP};
KW   Transmembrane {ECO:0000256|SAM:Phobius};
KW   Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT   SIGNAL          1..29
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT   CHAIN           30..502
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT                   /id="PRO_5014311924"
FT   TRANSMEM        478..496
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   DOMAIN          363..441
FT                   /note="SpaA"
FT                   /evidence="ECO:0000259|Pfam:PF17802"
FT   DOMAIN          461..499
FT                   /note="GRAM_POS_ANCHORING"
FT                   /evidence="ECO:0000259|Pfam:PF00746"
SQ   SEQUENCE   502 AA;  53450 MW;  E3B7F5603F6D6192 CRC64;
     MKKKMIQSLL VASLAFGMAV SPVTPIAFAA ETGTITVQDT QKGATYKAYK VFDAEIDNAN
     VSDSNKDGAS YLIPQGKEAE YKASTDFNSL FTTTTNGGRT YVTKKDTASA NEIATWAKSI
     SANTTPVSTV TESNNDGTEV INVSQYGYYY VSSTVNNGAV IMVTSVTPNA TIHEKNTDAT
     WGDGGGKTVD QKTYSVGDTV KYTITYKNAV NYHGTEKVYQ YVIKDTMPSA SVVDLNEGSY
     EVTITDGSGN ITTLTQGSEK ATGKYNLLEE NNNFTITIPW AATNTPTGNT QNGANDDFFY
     KGINTITVTY TGVLKSGAKP GSADLPENTN IATINPNTSN DDPGQKVTVR DGQITIKKID
     GSTKASLQGA IFVLKNATGQ FLNFNDTNNV EWGTEANATE YTTGADGIIT ITGLKEGTYY
     LVEKKAPLGY NLLDNSQKVI LGDGATDTTN SDNLLVNPTV ENNKGTELPS TGGIGTTIFY
     IIGAILVIGA GIVLVARRRL RS
//