ID CPEB4_MOUSE Reviewed; 729 AA. AC Q7TN98; A6H6G0; Q69ZD7; DT 12-DEC-2006, integrated into UniProtKB/Swiss-Prot. DT 01-OCT-2003, sequence version 1. DT 30-NOV-2016, entry version 96. DE RecName: Full=Cytoplasmic polyadenylation element-binding protein 4; DE Short=CPE-BP4; DE Short=CPE-binding protein 4; DE Short=mCPEB-4; GN Name=Cpeb4; Synonyms=Kiaa1673; OS Mus musculus (Mouse). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; OC Muroidea; Muridae; Murinae; Mus; Mus. OX NCBI_TaxID=10090; RN [1] RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2; 3 AND 4), ALTERNATIVE RP SPLICING, INDUCTION, AND TISSUE SPECIFICITY. RC STRAIN=BALB/cJ; TISSUE=Brain; RX PubMed=12871996; DOI=10.1073/pnas.1133424100; RA Theis M., Si K., Kandel E.R.; RT "Two previously undescribed members of the mouse CPEB family of genes RT and their inducible expression in the principal cell layers of the RT hippocampus."; RL Proc. Natl. Acad. Sci. U.S.A. 100:9602-9607(2003). RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 5). RC TISSUE=Brain; RX PubMed=15368895; DOI=10.1093/dnares/11.3.205; RA Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., RA Saga Y., Seino S., Nishimura M., Kaisho T., Hoshino K., Kitamura H., RA Nagase T., Ohara O., Koga H.; RT "Prediction of the coding sequences of mouse homologues of KIAA gene: RT IV. The complete nucleotide sequences of 500 mouse KIAA-homologous RT cDNAs identified by screening of terminal sequences of cDNA clones RT randomly sampled from size-fractionated libraries."; RL DNA Res. 11:205-218(2004). RN [3] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 5). RC TISSUE=Brain; RX PubMed=15489334; DOI=10.1101/gr.2596504; RG The MGC Project Team; RT "The status, quality, and expansion of the NIH full-length cDNA RT project: the Mammalian Gene Collection (MGC)."; RL Genome Res. 14:2121-2127(2004). RN [4] RP FUNCTION, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY. RX PubMed=17024188; DOI=10.1038/sj.emboj.7601322; RA Huang Y.S., Kan M.C., Lin C.L., Richter J.D.; RT "CPEB3 and CPEB4 in neurons: analysis of RNA-binding specificity and RT translational control of AMPA receptor GluR2 mRNA."; RL EMBO J. 25:4865-4876(2006). RN [5] RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-97, AND IDENTIFICATION RP BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. RX PubMed=19144319; DOI=10.1016/j.immuni.2008.11.006; RA Trost M., English L., Lemieux S., Courcelles M., Desjardins M., RA Thibault P.; RT "The phagosomal proteome in interferon-gamma-activated macrophages."; RL Immunity 30:143-154(2009). RN [6] RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-97 AND SER-99, AND RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. RC TISSUE=Brain, Kidney, and Spleen; RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001; RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R., RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.; RT "A tissue-specific atlas of mouse protein phosphorylation and RT expression."; RL Cell 143:1174-1189(2010). CC -!- FUNCTION: RNA-binding protein which, in contrast to CPEB1, does CC not bind to the cytoplasmic polyadenylation element (CPE), a CC uridine-rich sequence element within the mRNA 3'-UTR. CC {ECO:0000269|PubMed:17024188}. CC -!- SUBUNIT: Interacts with TOB1. {ECO:0000250|UniProtKB:Q17RY0}. CC -!- SUBCELLULAR LOCATION: Cell projection, dendrite CC {ECO:0000269|PubMed:17024188}. Cell junction, synapse, CC postsynaptic cell membrane, postsynaptic density CC {ECO:0000269|PubMed:17024188}. CC -!- ALTERNATIVE PRODUCTS: CC Event=Alternative splicing; Named isoforms=5; CC Name=1; Synonyms=mCPEB-4a; CC IsoId=Q7TN98-1; Sequence=Displayed; CC Name=2; Synonyms=mCPEB-4b; CC IsoId=Q7TN98-2; Sequence=VSP_022046; CC Name=3; Synonyms=mCPEB-4c; CC IsoId=Q7TN98-3; Sequence=VSP_022044; CC Name=4; Synonyms=mCPEB-4d; CC IsoId=Q7TN98-4; Sequence=VSP_022043; CC Name=5; CC IsoId=Q7TN98-5; Sequence=VSP_022045; CC -!- TISSUE SPECIFICITY: Highly expressed in brain (at protein level) CC (PubMed:17024188). In brain, expressed in the hippocampus and in CC granule and Purkinje cells of the cerebellum (at protein level) CC (PubMed:17024188). Strongly expressed in kidney, lung and heart. CC Weakly expressed in liver, spleen, ovary and in granular cells of CC dentate gyrus and the pyramidal cells of CA3 and CA1 of the CC hippocampus (PubMed:12871996). {ECO:0000269|PubMed:12871996, CC ECO:0000269|PubMed:17024188}. CC -!- INDUCTION: Up-regulated in granular cells of the dentate gyrus of CC CA1 and CA3 after kainate-induced seizures. CC {ECO:0000269|PubMed:12871996}. CC -!- SIMILARITY: Belongs to the RRM CPEB family. {ECO:0000305}. CC -!- SIMILARITY: Contains 2 RRM (RNA recognition motif) domains. CC {ECO:0000255|PROSITE-ProRule:PRU00176}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AY313775; AAQ20844.1; -; mRNA. DR EMBL; AK173229; BAD32507.1; -; mRNA. DR EMBL; BC115430; AAI15431.1; -; mRNA. DR EMBL; BC115431; AAI15432.1; -; mRNA. DR EMBL; BC145863; AAI45864.1; -; mRNA. DR EMBL; BC145865; AAI45866.1; -; mRNA. DR CCDS; CCDS24514.1; -. [Q7TN98-1] DR RefSeq; NP_001277607.1; NM_001290678.1. [Q7TN98-5] DR RefSeq; NP_080528.2; NM_026252.4. [Q7TN98-1] DR UniGene; Mm.339792; -. DR ProteinModelPortal; Q7TN98; -. DR SMR; Q7TN98; -. DR STRING; 10090.ENSMUSP00000020543; -. DR iPTMnet; Q7TN98; -. DR PhosphoSitePlus; Q7TN98; -. DR PaxDb; Q7TN98; -. DR PRIDE; Q7TN98; -. DR Ensembl; ENSMUST00000020543; ENSMUSP00000020543; ENSMUSG00000020300. [Q7TN98-1] DR GeneID; 67579; -. DR KEGG; mmu:67579; -. DR UCSC; uc007iir.2; mouse. [Q7TN98-2] DR UCSC; uc007iis.2; mouse. [Q7TN98-5] DR UCSC; uc007iit.2; mouse. [Q7TN98-1] DR CTD; 80315; -. DR MGI; MGI:1914829; Cpeb4. DR eggNOG; KOG0129; Eukaryota. DR eggNOG; ENOG410Y1XZ; LUCA. DR GeneTree; ENSGT00390000012886; -. DR HOGENOM; HOG000290660; -. DR HOVERGEN; HBG058010; -. DR InParanoid; Q7TN98; -. DR KO; K02602; -. DR OMA; TGFNNWS; -. DR OrthoDB; EOG091G0AO9; -. DR PhylomeDB; Q7TN98; -. DR TreeFam; TF317658; -. DR PRO; PR:Q7TN98; -. DR Proteomes; UP000000589; Chromosome 11. DR Bgee; ENSMUSG00000020300; -. DR ExpressionAtlas; Q7TN98; baseline and differential. DR GO; GO:0030054; C:cell junction; IEA:UniProtKB-KW. DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB. DR GO; GO:0030425; C:dendrite; IDA:UniProtKB. DR GO; GO:0005783; C:endoplasmic reticulum; IDA:UniProtKB. DR GO; GO:1990124; C:messenger ribonucleoprotein complex; IBA:GO_Central. DR GO; GO:0005634; C:nucleus; IDA:UniProtKB. DR GO; GO:0014069; C:postsynaptic density; IDA:UniProtKB. DR GO; GO:0045211; C:postsynaptic membrane; IEA:UniProtKB-KW. DR GO; GO:0045202; C:synapse; IDA:MGI. DR GO; GO:0003730; F:mRNA 3'-UTR binding; IBA:GO_Central. DR GO; GO:0000166; F:nucleotide binding; IEA:InterPro. DR GO; GO:0044822; F:poly(A) RNA binding; ISO:MGI. DR GO; GO:0043022; F:ribosome binding; IBA:GO_Central. DR GO; GO:0003723; F:RNA binding; IDA:MGI. DR GO; GO:0008135; F:translation factor activity, RNA binding; IBA:GO_Central. DR GO; GO:0000900; F:translation repressor activity, nucleic acid binding; IBA:GO_Central. DR GO; GO:0071230; P:cellular response to amino acid stimulus; IDA:UniProtKB. DR GO; GO:0036294; P:cellular response to decreased oxygen levels; ISS:UniProtKB. DR GO; GO:0042149; P:cellular response to glucose starvation; ISS:UniProtKB. DR GO; GO:0035235; P:ionotropic glutamate receptor signaling pathway; ISS:UniProtKB. DR GO; GO:2000766; P:negative regulation of cytoplasmic translation; IBA:GO_Central. DR GO; GO:0043524; P:negative regulation of neuron apoptotic process; ISS:UniProtKB. DR GO; GO:0002931; P:response to ischemia; IDA:UniProtKB. DR Gene3D; 3.30.70.330; -; 3. DR InterPro; IPR032296; CEBP_ZZ. DR InterPro; IPR012677; Nucleotide-bd_a/b_plait. DR InterPro; IPR000504; RRM_dom. DR Pfam; PF16366; CEBP_ZZ; 1. DR Pfam; PF16367; RRM_7; 1. DR SMART; SM00360; RRM; 2. DR SUPFAM; SSF54928; SSF54928; 1. DR PROSITE; PS50102; RRM; 2. PE 1: Evidence at protein level; KW Alternative splicing; Cell junction; Cell membrane; Cell projection; KW Complete proteome; Membrane; Phosphoprotein; KW Postsynaptic cell membrane; Reference proteome; Repeat; RNA-binding; KW Synapse. FT CHAIN 1 729 Cytoplasmic polyadenylation element- FT binding protein 4. FT /FTId=PRO_0000269265. FT DOMAIN 472 563 RRM 1. {ECO:0000255|PROSITE- FT ProRule:PRU00176}. FT DOMAIN 580 662 RRM 2. {ECO:0000255|PROSITE- FT ProRule:PRU00176}. FT COMPBIAS 232 246 His-rich. FT COMPBIAS 302 310 Poly-Gly. FT MOD_RES 97 97 Phosphoserine. FT {ECO:0000244|PubMed:19144319, FT ECO:0000244|PubMed:21183079}. FT MOD_RES 99 99 Phosphoserine. FT {ECO:0000244|PubMed:21183079}. FT MOD_RES 137 137 Phosphoserine. FT {ECO:0000250|UniProtKB:Q17RY0}. FT MOD_RES 252 252 Phosphoserine. FT {ECO:0000250|UniProtKB:Q17RY0}. FT MOD_RES 255 255 Phosphoserine. FT {ECO:0000250|UniProtKB:Q17RY0}. FT MOD_RES 326 326 Phosphothreonine. FT {ECO:0000250|UniProtKB:Q17RY0}. FT MOD_RES 330 330 Phosphoserine. FT {ECO:0000250|UniProtKB:Q17RY0}. FT MOD_RES 332 332 Phosphoserine. FT {ECO:0000250|UniProtKB:Q17RY0}. FT VAR_SEQ 402 427 Missing (in isoform 4). FT {ECO:0000303|PubMed:12871996}. FT /FTId=VSP_022043. FT VAR_SEQ 402 419 Missing (in isoform 3). FT {ECO:0000303|PubMed:12871996}. FT /FTId=VSP_022044. FT VAR_SEQ 404 428 Missing (in isoform 5). FT {ECO:0000303|PubMed:15368895, FT ECO:0000303|PubMed:15489334}. FT /FTId=VSP_022045. FT VAR_SEQ 420 427 Missing (in isoform 2). FT {ECO:0000303|PubMed:12871996}. FT /FTId=VSP_022046. SQ SEQUENCE 729 AA; 80122 MW; 7CB042EDE4B7C0CA CRC64; MGDYGFGVLV QSNTGNKSAF PVRFHPHLQP PHHHQNATPN PAAFINNNTA ANGSSAGSAW LFPAPATHNI QDEILGSEKA KSQQQEQQDP LEKQQLSPSP GQEAGILPET EKAKAEENPG DSSSENSNGK EKLRIESPVL TGFDYQEATG LGTSTQPLTS SASSLTGFSN WSAAIAPSSS TIINEDASFF HQGGVPGASA NNGALLFQNF PHHVSPGFGG SFSPQIGPLS QHHPHHPHFQ HHHSQHQQQR RSPASPHPPP FTHRSAAFNQ LPHLANNLNK PPSPWSSYQS PSPTPSSSWS PGGGGYGGWG ASQGRDHRRG LNGGITPLNS ISPLKKNFAS NHIQLQKYAR PSSAFAPKSW MEDSLNRADN IFPFPERPRT FDMHSLESSL IDIMRAENDS IKGRLNYSYP GSDSSLLINA RTYGRRRGQS SLFPMEDGFL DDGRGDQPLH SGLGSPHCFT HQNGERVERY SRKVFVGGLP PDIDEDEITA SFRRFGPLIV DWPHKAESKS YFPPKGYAFL LFQDESSVQA LIDACIEEDG KLYLCVSSPT IKDKPVQIRP WNLSDSDFVM DGSQPLDPRK TIFVGGVPRP LRAVELAMIM DRLYGGVCYA GIDTDPELKY PKGAGRVAFS NQQSYIAAIS ARFVQLQHGE IDKRVEVKPY VLDDQLCDEC QGARCGGKFA PFFCANVTCL QYYCEYCWAA IHSRAGREFH KPLVKEGGDR PRHISFRWN //