ID DPH1_ASHGO Reviewed; 426 AA. AC Q75AZ9; DT 20-DEC-2005, integrated into UniProtKB/Swiss-Prot. DT 05-JUL-2004, sequence version 1. DT 13-NOV-2013, entry version 58. DE RecName: Full=Diphthamide biosynthesis protein 1; GN Name=DPH1; OrderedLocusNames=ADL229W; OS Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL OS Y-1056) (Yeast) (Eremothecium gossypii). OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; OC Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Eremothecium. OX NCBI_TaxID=284811; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056; RX PubMed=15001715; DOI=10.1126/science.1095781; RA Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S., RA Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A., RA Gaffney T.D., Philippsen P.; RT "The Ashbya gossypii genome as a tool for mapping the ancient RT Saccharomyces cerevisiae genome."; RL Science 304:304-307(2004). CC -!- FUNCTION: Required for the first step of diphthamide biosynthesis, CC the transfer of 3-amino-3-carboxypropyl from S-adenosyl-L- CC methionine to a histidine residue. Diphthamide is a post- CC translational modification of histidine which occurs in elongation CC factor 2 (By similarity). CC -!- PATHWAY: Protein modification; peptidyl-diphthamide biosynthesis. CC -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity). CC -!- SIMILARITY: Belongs to the DPH1/DPH2 family. DPH1 subfamily. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AE016817; AAS51691.1; -; Genomic_DNA. DR RefSeq; NP_983867.1; NM_209220.1. DR ProteinModelPortal; Q75AZ9; -. DR STRING; 33169.AGOS_ADL229W; -. DR EnsemblFungi; AAS51691; AAS51691; AGOS_ADL229W. DR GeneID; 4620002; -. DR KEGG; ago:AGOS_ADL229W; -. DR eggNOG; COG1736; -. DR HOGENOM; HOG000164743; -. DR OMA; DILTQFC; -. DR OrthoDB; EOG718KPK; -. DR PhylomeDB; Q75AZ9; -. DR UniPathway; UPA00559; -. DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell. DR GO; GO:0017183; P:peptidyl-diphthamide biosynthetic process from peptidyl-histidine; IEA:UniProtKB-UniPathway. DR InterPro; IPR002728; DPH1/DPH2. DR InterPro; IPR016435; eu_DPH1/arc_DPH2. DR PANTHER; PTHR10762; PTHR10762; 1. DR PANTHER; PTHR10762:SF1; PTHR10762:SF1; 1. DR Pfam; PF01866; Diphthamide_syn; 1. DR PIRSF; PIRSF004967; DPH1; 1. DR TIGRFAMs; TIGR00322; diphth2_R; 1. PE 3: Inferred from homology; KW Complete proteome; Cytoplasm; Reference proteome. FT CHAIN 1 426 Diphthamide biosynthesis protein 1. FT /FTId=PRO_0000083367. SQ SEQUENCE 426 AA; 48184 MW; F080D8449B600DDD CRC64; MSTADEVTKK PRRRFVGVSR GENKVSTKQV SGDEAKELVR SAKSNTGARR AINQIPDEIL NDENLQEAIK LLPSNYNFEI HKTVWNIRKH NAKRVALQMP EGLLIYSLVI SDILEQFCNC ETVVMGDVSY GACCIDDFTA RALGCDFIVH YAHSCLVPVD VTSIKILYVF VTIGIDEDHL MKTLQKNFAK GTSLAVFGTI QFNPAIHSIR EKLLYSESHM LYITPPQIKP LSKGEVLGCT SQRLPKEQFA AMVYVGDGRF HLESAMIHNP DIPAFRYDPY SRKFTRETYD QHQLVEVRSS AIEKARNSQC FGLILGALGR QGNLATVANL EKKLRAAGKK VVRIILSEIF PQKLAMFDHI DAFVQVACPR LSIDWGYAFN KPLLTPYETN VMLGQDRMFN EKYYPMDYYE VNGYGRGKQP THDNVI //