ID QUEE_GEOSL Reviewed; 250 AA. AC Q74CF3; DT 21-MAR-2012, integrated into UniProtKB/Swiss-Prot. DT 05-JUL-2004, sequence version 1. DT 16-OCT-2013, entry version 54. DE RecName: Full=7-carboxy-7-deazaguanine synthase; DE Short=CDG synthase; DE EC=4.3.99.3; DE AltName: Full=Queuosine biosynthesis protein QueE; GN Name=queE; OrderedLocusNames=GSU1721; OS Geobacter sulfurreducens (strain ATCC 51573 / DSM 12127 / PCA). OC Bacteria; Proteobacteria; Deltaproteobacteria; Desulfuromonadales; OC Geobacteraceae; Geobacter. OX NCBI_TaxID=243231; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=ATCC 51573 / DSM 12127 / PCA; RX PubMed=14671304; DOI=10.1126/science.1088727; RA Methe B.A., Nelson K.E., Eisen J.A., Paulsen I.T., Nelson W.C., RA Heidelberg J.F., Wu D., Wu M., Ward N.L., Beanan M.J., Dodson R.J., RA Madupu R., Brinkac L.M., Daugherty S.C., DeBoy R.T., Durkin A.S., RA Gwinn M.L., Kolonay J.F., Sullivan S.A., Haft D.H., Selengut J., RA Davidsen T.M., Zafar N., White O., Tran B., Romero C., Forberger H.A., RA Weidman J.F., Khouri H.M., Feldblyum T.V., Utterback T.R., RA Van Aken S.E., Lovley D.R., Fraser C.M.; RT "Genome of Geobacter sulfurreducens: metal reduction in subsurface RT environments."; RL Science 302:1967-1969(2003). CC -!- FUNCTION: Catalyzes the conversion of 6-carboxy-5,6,7,8- CC tetrahydropterin (CPH4) to 7-carboxy-7-deazaguanine (CDG) (By CC similarity). CC -!- CATALYTIC ACTIVITY: 6-carboxy-5,6,7,8-tetrahydropterin = 7- CC carboxy-7-carbaguanine + NH(3). CC -!- COFACTOR: Binds 1 4Fe-4S cluster. The cluster is coordinated with CC 3 cysteines and an exchangeable S-adenosyl-L-methionine (By CC similarity). CC -!- COFACTOR: Binds 1 S-adenosyl-L-methionine per subunit (By CC similarity). CC -!- PATHWAY: Purine metabolism; 7-cyano-7-deazaguanine biosynthesis. CC -!- SIMILARITY: Belongs to the radical SAM superfamily. 7-carboxy-7- CC deazaguanine synthase family. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AE017180; AAR35098.1; -; Genomic_DNA. DR RefSeq; NP_952771.1; NC_002939.5. DR ProteinModelPortal; Q74CF3; -. DR STRING; 243231.GSU1721; -. DR DNASU; 2687051; -. DR EnsemblBacteria; AAR35098; AAR35098; GSU1721. DR GeneID; 2687051; -. DR KEGG; gsu:GSU1721; -. DR PATRIC; 22026289; VBIGeoSul17553_1762. DR HOGENOM; HOG000266147; -. DR OMA; RFIFVRF; -. DR ProtClustDB; CLSK828502; -. DR BioCyc; GSUL243231:GH27-1739-MONOMER; -. DR UniPathway; UPA00391; -. DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW. DR GO; GO:0016840; F:carbon-nitrogen lyase activity; IEA:UniProtKB-HAMAP. DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW. DR GO; GO:0008616; P:queuosine biosynthetic process; IEA:UniProtKB-HAMAP. DR Gene3D; 3.20.20.70; -; 1. DR HAMAP; MF_00917; QueE; 1; -. DR InterPro; IPR024924; 7-CO-7-deazaguanine_synth-like. DR InterPro; IPR013785; Aldolase_TIM. DR InterPro; IPR007197; rSAM. DR Pfam; PF04055; Radical_SAM; 1. DR PIRSF; PIRSF000370; QueE; 1. PE 3: Inferred from homology; KW 4Fe-4S; Complete proteome; Iron; Iron-sulfur; Lyase; Metal-binding; KW Queuosine biosynthesis; Reference proteome; S-adenosyl-L-methionine. FT CHAIN 1 250 7-carboxy-7-deazaguanine synthase. FT /FTId=PRO_0000416205. FT METAL 34 34 Iron-sulfur (4Fe-4S-S-AdoMet) (By FT similarity). FT METAL 38 38 Iron-sulfur (4Fe-4S-S-AdoMet) (By FT similarity). FT METAL 41 41 Iron-sulfur (4Fe-4S-S-AdoMet) (By FT similarity). SQ SEQUENCE 250 AA; 27879 MW; 3BED339ECD30FD30 CRC64; MSKPGAELEE VFSSVQGEGM LIGLRQVFIR FRGCNLTCDY CDTPAGTPAE PCRIEQTPGR RDFVPADNPV SLDRVAALVE GWQRGWPGVH DSISITGGEP LLRHDILMQW LPVLREHLPV YLETNGVMHA ALGLVINHVD IIGMDIKIPS TSGCTDLWDD HRQFLEIANT RRAFIKIVVG EETEDWEITR ASEIIAGVNR DIPLILQPVT RAGDTLGIKP VKALELQELA CRYLAEVRII PQTHRFMGQL //