ID Q70U40_9ENTR Unreviewed; 336 AA. AC Q70U40; DT 06-JUL-2016, integrated into UniProtKB/TrEMBL. DT 06-JUL-2016, sequence version 1. DT 03-MAY-2023, entry version 23. DE RecName: Full=DNA-directed RNA polymerase {ECO:0000256|ARBA:ARBA00012418}; DE EC=2.7.7.6 {ECO:0000256|ARBA:ARBA00012418}; DE Flags: Fragment; GN Name=rpoB {ECO:0000313|EMBL:CAD66110.1}; OS Enterobacter hormaechei subsp. oharae. OC Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; OC Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex. OX NCBI_TaxID=301102 {ECO:0000313|EMBL:CAD66110.1}; RN [1] {ECO:0000313|EMBL:CAD66110.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=Type strain:EN-314 {ECO:0000313|EMBL:CAD66110.1}; RX PubMed=12957918; DOI=10.1128/AEM.69.9.5306-5318.2003; RA Hoffmann H., Roggenkamp A.; RT "Population genetics of the nomenspecies Enterobacter cloacae."; RL Appl. Environ. Microbiol. 69:5306-5318(2003). CC -!- CATALYTIC ACTIVITY: CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA- CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395; CC EC=2.7.7.6; Evidence={ECO:0000256|ARBA:ARBA00024550}; CC -!- SIMILARITY: Belongs to the RNA polymerase beta chain family. CC {ECO:0000256|RuleBase:RU000434}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AJ543677; CAD66110.1; -; Genomic_DNA. DR AlphaFoldDB; Q70U40; -. DR SMR; Q70U40; -. DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW. DR GO; GO:0003677; F:DNA binding; IEA:InterPro. DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-EC. DR GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro. DR GO; GO:0006351; P:DNA-templated transcription; IEA:InterPro. DR Gene3D; 2.40.50.100; -; 1. DR Gene3D; 3.90.1100.10; -; 1. DR Gene3D; 2.30.150.10; DNA-directed RNA polymerase, beta subunit, external 1 domain; 1. DR InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf. DR InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1. DR InterPro; IPR015712; DNA-dir_RNA_pol_su2. DR InterPro; IPR007120; DNA-dir_RNAP_su2_dom. DR InterPro; IPR007645; RNA_pol_Rpb2_3. DR PANTHER; PTHR20856; DNA-DIRECTED RNA POLYMERASE I SUBUNIT 2; 1. DR PANTHER; PTHR20856:SF20; DNA-DIRECTED RNA POLYMERASE SUBUNIT BETA; 1. DR Pfam; PF04565; RNA_pol_Rpb2_3; 1. DR Pfam; PF10385; RNA_pol_Rpb2_45; 1. DR Pfam; PF00562; RNA_pol_Rpb2_6; 1. DR SUPFAM; SSF64484; beta and beta-prime subunits of DNA dependent RNA-polymerase; 1. PE 3: Inferred from homology; KW DNA-directed RNA polymerase {ECO:0000256|ARBA:ARBA00022478}; KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695}; KW Transcription {ECO:0000256|ARBA:ARBA00023163}; KW Transferase {ECO:0000256|ARBA:ARBA00022679}. FT DOMAIN 39..106 FT /note="RNA polymerase Rpb2" FT /evidence="ECO:0000259|Pfam:PF04565" FT DOMAIN 117..182 FT /note="DNA-directed RNA polymerase beta subunit external 1" FT /evidence="ECO:0000259|Pfam:PF10385" FT DOMAIN 243..336 FT /note="DNA-directed RNA polymerase subunit 2 hybrid- FT binding" FT /evidence="ECO:0000259|Pfam:PF00562" FT NON_TER 1 FT /evidence="ECO:0000313|EMBL:CAD66110.1" FT NON_TER 336 FT /evidence="ECO:0000313|EMBL:CAD66110.1" SQ SEQUENCE 336 AA; 36768 MW; 47126E08B8C788C2 CRC64; VKERLSLGDL DTLMPQDMIN AKPISAAVKE FFGSSQLSQF MDQNNPLSEI THKRRISALG PGGLTRERAG FEVRDVHPTH YGRVCPIETP EGPNIGLINS LSVYAQTNEY GFLETPYRKV TDGVVTDEIH YLSAIEEGNY VIAQANSNLD DEGHFVEDLV TCRSKGESSL FSRDQVDYMD VSTQQVVSVG ASLIPFLEHD DANRALMGAN MQRQAVPTLR ADKPLVGTGM ERAVAVDSGV TAVAKRGGTV QYVDASRIVI KVNEDEMYPG EAGIDIYNLT KYTRSNQNTC INQMPCVSLG EPVERGDVLA DGPSTDLGEL ALGQNMRVAF MPWNGY //