ID S35A3_BOVIN Reviewed; 326 AA. AC Q6YC49; A7Z022; DT 15-FEB-2005, integrated into UniProtKB/Swiss-Prot. DT 05-JUL-2004, sequence version 1. DT 03-MAY-2023, entry version 119. DE RecName: Full=UDP-N-acetylglucosamine transporter; DE AltName: Full=Golgi UDP-GlcNAc transporter; DE AltName: Full=Solute carrier family 35 member A3; GN Name=SLC35A3; OS Bos taurus (Bovine). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae; OC Bovinae; Bos. OX NCBI_TaxID=9913; RN [1] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], DISEASE, VARIANT PHE-180, AND FUNCTION. RX PubMed=16344554; DOI=10.1101/gr.3690506; RA Thomsen B., Horn P., Panitz F., Bendixen E., Petersen A.H., Holm L.E., RA Nielsen V.H., Agerholm J.S., Arnbjerg J., Bendixen C.; RT "A missense mutation in the bovine SLC35A3 gene, encoding a UDP-N- RT acetylglucosamine transporter, causes complex vertebral malformation."; RL Genome Res. 16:97-105(2006). RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RC STRAIN=Hereford; TISSUE=Hypothalamus; RG NIH - Mammalian Gene Collection (MGC) project; RL Submitted (SEP-2007) to the EMBL/GenBank/DDBJ databases. CC -!- FUNCTION: Transports diphosphate-N-acetylglucosamine (UDP-GlcNAc) from CC the cytosol into the lumen of the Golgi apparatus, functioning as an CC antiporter that exchanges UDP-N-acetyl-alpha-D-glucosamine for UMP (By CC similarity). May supply UDP-GlcNAc as substrate for Golgi-resident CC glycosyltransferases that generate highly branched, multiantennary CC complex N-glycans and keratan sulfate (PubMed:16344554). However, the CC exact role of SLC35A3 still needs to be elucidated, it could be a CC member of a catalytically more efficient multiprotein complex rather CC than function independently as a single transporter (By similarity). CC {ECO:0000250|UniProtKB:Q9Y2D2, ECO:0000269|PubMed:16344554}. CC -!- CATALYTIC ACTIVITY: CC Reaction=UDP-N-acetyl-alpha-D-glucosamine(in) + UMP(out) = UDP-N- CC acetyl-alpha-D-glucosamine(out) + UMP(in); Xref=Rhea:RHEA:72695, CC ChEBI:CHEBI:57705, ChEBI:CHEBI:57865; CC Evidence={ECO:0000250|UniProtKB:Q9Y2D2}; CC -!- SUBUNIT: Interacts with SLC35A2; the interaction is reduced in the CC presence of SLC35A4. Found in a complex with SLC35A2 and SLC35A4. CC Interacts with MGAT4B. {ECO:0000250|UniProtKB:Q9Y2D2}. CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane CC {ECO:0000250|UniProtKB:Q9Y2D2}; Multi-pass membrane protein CC {ECO:0000255}. CC -!- PTM: O-Glcnacylation regulates the stability of SLC35A3 and the CC specific complex formation with MGAT4B. {ECO:0000250|UniProtKB:Q9Y2D2}. CC -!- DISEASE: Note=Defects in SLC35A3 may be cause of complex vertebral CC malformation (CVM) in Holstein. {ECO:0000269|PubMed:16344554}. CC -!- SIMILARITY: Belongs to the nucleotide-sugar transporter family. SLC35A CC subfamily. {ECO:0000305}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AY160683; AAO22138.1; -; Genomic_DNA. DR EMBL; BC153220; AAI53221.1; -; mRNA. DR RefSeq; NP_001098856.1; NM_001105386.1. DR RefSeq; XP_005204177.1; XM_005204120.2. DR RefSeq; XP_015319233.1; XM_015463747.1. DR AlphaFoldDB; Q6YC49; -. DR SMR; Q6YC49; -. DR STRING; 9913.ENSBTAP00000016519; -. DR PaxDb; Q6YC49; -. DR Ensembl; ENSBTAT00000016519.4; ENSBTAP00000016519.3; ENSBTAG00000012454.4. DR Ensembl; ENSBTAT00000076918.1; ENSBTAP00000063794.1; ENSBTAG00000012454.4. DR GeneID; 520918; -. DR KEGG; bta:520918; -. DR CTD; 23443; -. DR VEuPathDB; HostDB:ENSBTAG00000012454; -. DR VGNC; VGNC:49969; SLC35A3. DR eggNOG; KOG2234; Eukaryota. DR GeneTree; ENSGT00950000182827; -. DR HOGENOM; CLU_024645_1_0_1; -. DR InParanoid; Q6YC49; -. DR OMA; VPAIMYV; -. DR TreeFam; TF315345; -. DR Reactome; R-BTA-727802; Transport of nucleotide sugars. DR Proteomes; UP000009136; Chromosome 3. DR Bgee; ENSBTAG00000012454; Expressed in spiral colon and 109 other tissues. DR ExpressionAtlas; Q6YC49; baseline and differential. DR GO; GO:0000139; C:Golgi membrane; ISS:UniProtKB. DR GO; GO:0005459; F:UDP-galactose transmembrane transporter activity; IBA:GO_Central. DR GO; GO:0008643; P:carbohydrate transport; IEA:UniProtKB-KW. DR GO; GO:1990569; P:UDP-N-acetylglucosamine transmembrane transport; ISS:UniProtKB. DR InterPro; IPR007271; Nuc_sug_transpt. DR PANTHER; PTHR10231; NUCLEOTIDE-SUGAR TRANSMEMBRANE TRANSPORTER; 1. DR PANTHER; PTHR10231:SF36; UDP-N-ACETYLGLUCOSAMINE TRANSPORTER; 1. DR Pfam; PF04142; Nuc_sug_transp; 1. DR PIRSF; PIRSF005799; UDP-gal_transpt; 1. DR SUPFAM; SSF103481; Multidrug resistance efflux transporter EmrE; 1. DR TIGRFAMs; TIGR00803; nst; 1. PE 1: Evidence at protein level; KW Antiport; Disease variant; Golgi apparatus; Membrane; Reference proteome; KW Sugar transport; Transmembrane; Transmembrane helix; Transport. FT CHAIN 1..326 FT /note="UDP-N-acetylglucosamine transporter" FT /id="PRO_0000213355" FT TRANSMEM 4..24 FT /note="Helical" FT /evidence="ECO:0000255" FT TRANSMEM 38..58 FT /note="Helical" FT /evidence="ECO:0000255" FT TRANSMEM 136..156 FT /note="Helical" FT /evidence="ECO:0000255" FT TRANSMEM 174..194 FT /note="Helical" FT /evidence="ECO:0000255" FT TRANSMEM 212..232 FT /note="Helical" FT /evidence="ECO:0000255" FT TRANSMEM 244..264 FT /note="Helical" FT /evidence="ECO:0000255" FT TRANSMEM 269..289 FT /note="Helical" FT /evidence="ECO:0000255" FT TRANSMEM 293..313 FT /note="Helical" FT /evidence="ECO:0000255" FT VARIANT 180 FT /note="V -> F (found in CVM; Holstein cattle. Does not FT rescue defective transport of UDP-N-acetylglucosamine into FT Golgi lumen in SLC35A3-deficient cells)" FT /evidence="ECO:0000269|PubMed:16344554" SQ SEQUENCE 326 AA; 36067 MW; 31F34B10BFB3DC37 CRC64; MSANLKYLSL GILVFQTTSL VLTMRYSRTL KEEGPRYLSS TAVVVAELLK IMACILLVYK DSKCSLRALN RILHDEILNK PMETLKLAIP SGIYTLQNNL LYVALSNLDA ATYQVTYQLK ILTTALFSVS MLSKKLGVYQ WLSLVILMTG VAFVQWPSDS QELNSKELSA GSQFVGLMAV LTACFSSGFA GVYFEKILKE TKQSVWIRNI QLGFFGSIFG LMGVYVYDGE LVSKNGFFQG YNRLTWIVVV LQALGGLVIA AVIKYADNIL KGFATSLSII LSTLISYFWL QDFVPTSVFF LGAILVITAT FLYGYDPKPA GNPTKA //