ID CYB_GENJO Reviewed; 379 AA. AC Q6XBW5; DT 31-OCT-2006, integrated into UniProtKB/Swiss-Prot. DT 05-JUL-2004, sequence version 1. DT 02-NOV-2016, entry version 58. DE RecName: Full=Cytochrome b; DE AltName: Full=Complex III subunit 3; DE AltName: Full=Complex III subunit III; DE AltName: Full=Cytochrome b-c1 complex subunit 3; DE AltName: Full=Ubiquinol-cytochrome-c reductase complex cytochrome b subunit; GN Name=MT-CYB; Synonyms=COB, CYTB, MTCYB; OS Genetta johnstoni (Johnston's genet). OG Mitochondrion. OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Mammalia; Eutheria; Laurasiatheria; Carnivora; Feliformia; Viverridae; OC Viverrinae; Genetta. OX NCBI_TaxID=205594; RN [1] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RA Gaubert P., Fernandes C.A., Bruford M.W., Veron G.; RT "Genets (Carnivora, Viverridae) in Africa: an evolutionary synthesis RT based on cytochrome b sequences and morphological characters."; RL Biol. J. Linn. Soc. Lond. 81:589-610(2004). RN [2] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RA Gaubert P., Tranier M., Delmas A.-S., Colyn M., Veron G.; RT "First molecular evidence for reassessing phylogenetic affinities RT between genets (Genetta) and the enigmatic genet-like taxa RT Osbornictis, Poiana and Prionodon (Carnivora, Viverridae)."; RL Zool. Scr. 33:117-129(2004). CC -!- FUNCTION: Component of the ubiquinol-cytochrome c reductase CC complex (complex III or cytochrome b-c1 complex) that is part of CC the mitochondrial respiratory chain. The b-c1 complex mediates CC electron transfer from ubiquinol to cytochrome c. Contributes to CC the generation of a proton gradient across the mitochondrial CC membrane that is then used for ATP synthesis. CC {ECO:0000250|UniProtKB:P00157}. CC -!- COFACTOR: CC Name=heme; Xref=ChEBI:CHEBI:30413; CC Evidence={ECO:0000250|UniProtKB:P00157}; CC Note=Binds 2 heme groups non-covalently. CC {ECO:0000250|UniProtKB:P00157}; CC -!- SUBUNIT: The cytochrome bc1 complex contains 11 subunits: 3 CC respiratory subunits (MT-CYB, CYC1 and UQCRFS1), 2 core proteins CC (UQCRC1 and UQCRC2) and 6 low-molecular weight proteins CC (UQCRH/QCR6, UQCRB/QCR7, UQCRQ/QCR8, UQCR10/QCR9, UQCR11/QCR10 and CC a cleavage product of UQCRFS1). This cytochrome bc1 complex then CC forms a dimer. {ECO:0000250|UniProtKB:P00157}. CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane CC {ECO:0000250|UniProtKB:P00157}; Multi-pass membrane protein CC {ECO:0000250|UniProtKB:P00157}. CC -!- MISCELLANEOUS: Heme 1 (or BL or b562) is low-potential and absorbs CC at about 562 nm, and heme 2 (or BH or b566) is high-potential and CC absorbs at about 566 nm. {ECO:0000250}. CC -!- SIMILARITY: Belongs to the cytochrome b family. CC {ECO:0000255|PROSITE-ProRule:PRU00967, ECO:0000255|PROSITE- CC ProRule:PRU00968}. CC -!- CAUTION: The full-length protein contains only eight transmembrane CC helices, not nine as predicted by bioinformatics tools. CC {ECO:0000250|UniProtKB:P00157}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AY241892; AAP73012.1; -; Genomic_DNA. DR EMBL; AF511051; AAQ08027.1; -; Genomic_DNA. DR ProteinModelPortal; Q6XBW5; -. DR HOVERGEN; HBG017694; -. DR GO; GO:0005743; C:mitochondrial inner membrane; IEA:UniProtKB-SubCell. DR GO; GO:0045275; C:respiratory chain complex III; IEA:InterPro. DR GO; GO:0009055; F:electron carrier activity; IEA:InterPro. DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW. DR GO; GO:0008121; F:ubiquinol-cytochrome-c reductase activity; IEA:InterPro. DR GO; GO:0006122; P:mitochondrial electron transport, ubiquinol to cytochrome c; IEA:InterPro. DR CDD; cd00284; Cytochrome_b_N; 1. DR Gene3D; 1.20.810.10; -; 1. DR InterPro; IPR005798; Cyt_b/b6_C. DR InterPro; IPR005797; Cyt_b/b6_N. DR InterPro; IPR027387; Cytb/b6-like. DR InterPro; IPR030689; Cytochrome_b. DR InterPro; IPR016174; Di-haem_cyt_TM. DR Pfam; PF00032; Cytochrom_B_C; 1. DR Pfam; PF00033; Cytochrome_B; 1. DR PIRSF; PIRSF038885; COB; 1. DR SUPFAM; SSF81342; SSF81342; 1. DR SUPFAM; SSF81648; SSF81648; 1. DR PROSITE; PS51003; CYTB_CTER; 1. DR PROSITE; PS51002; CYTB_NTER; 1. PE 3: Inferred from homology; KW Electron transport; Heme; Iron; Membrane; Metal-binding; KW Mitochondrion; Mitochondrion inner membrane; Respiratory chain; KW Transmembrane; Transmembrane helix; Transport; Ubiquinone. FT CHAIN 1 379 Cytochrome b. FT /FTId=PRO_0000254693. FT TRANSMEM 33 53 Helical. {ECO:0000250|UniProtKB:P00157}. FT TRANSMEM 77 98 Helical. {ECO:0000250|UniProtKB:P00157}. FT TRANSMEM 113 133 Helical. {ECO:0000250|UniProtKB:P00157}. FT TRANSMEM 178 198 Helical. {ECO:0000250|UniProtKB:P00157}. FT TRANSMEM 226 246 Helical. {ECO:0000250|UniProtKB:P00157}. FT TRANSMEM 288 308 Helical. {ECO:0000250|UniProtKB:P00157}. FT TRANSMEM 320 340 Helical. {ECO:0000250|UniProtKB:P00157}. FT TRANSMEM 347 367 Helical. {ECO:0000250|UniProtKB:P00157}. FT METAL 83 83 Iron 1 (heme b562 axial ligand). FT {ECO:0000250|UniProtKB:P00157}. FT METAL 97 97 Iron 2 (heme b566 axial ligand). FT {ECO:0000250|UniProtKB:P00157}. FT METAL 182 182 Iron 1 (heme b562 axial ligand). FT {ECO:0000250|UniProtKB:P00157}. FT METAL 196 196 Iron 2 (heme b566 axial ligand). FT {ECO:0000250|UniProtKB:P00157}. FT BINDING 201 201 Ubiquinone. FT {ECO:0000250|UniProtKB:P00157}. SQ SEQUENCE 379 AA; 42713 MW; E836C5FE03D94932 CRC64; MTDIRKSHPL AKIINESFID LPAPSNISAW WNFGSLLGVC LIIQILTGLF LAMHYTSDTM TAFSSVTHIC RDVNYGWIIR YMHANGASMF FICLFMHAGR GVYYGSYTFT ETWNIGILLM FTVMATAFMG YVLPWGQMSF WGATVITNLL SAIPYIGTNL VEWIWGGFSV DKATLTRLFA FHFILPFIIS ALAAVHLLFL HETGSNNPSG MMSDSDKIPF HPYYTIKDIL GILLLILVLM LLVLFSPDLL GDPDNYIPAN PLNTPPHIKP EWYFLFAYAI LRSIPNKLGG VLALVLSILI LAIIPFLHTS KQRSMMFRPL SQCLFWLLVA DLLTLTWIGG QPVEHPFITI GQLASILYFS IFLILMPVSG IIENRLLKW //