ID Q6TNQ8_DANRE Unreviewed; 565 AA. AC Q6TNQ8; DT 05-JUL-2004, integrated into UniProtKB/TrEMBL. DT 05-JUL-2004, sequence version 1. DT 29-MAY-2024, entry version 116. DE RecName: Full=Branchpoint-bridging protein {ECO:0000256|RuleBase:RU367126}; GN Name=sf1 {ECO:0000313|RefSeq:NP_997818.1, GN ECO:0000313|ZFIN:ZDB-GENE-030131-2492}; GN Synonyms=SF1 {ECO:0000313|EMBL:AAQ91271.1}, wu:fc09f06 GN {ECO:0000313|RefSeq:NP_997818.1}, znf162 GN {ECO:0000313|RefSeq:NP_997818.1}; OS Danio rerio (Zebrafish) (Brachydanio rerio). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes; OC Danionidae; Danioninae; Danio. OX NCBI_TaxID=7955 {ECO:0000313|EMBL:AAQ91271.1}; RN [1] {ECO:0000313|RefSeq:NP_997818.1} RP NUCLEOTIDE SEQUENCE. RX PubMed=11116086; RA Woods I.G., Kelly P.D., Chu F., Ngo-Hazelett P., Yan Y.L., Huang H., RA Postlethwait J.H., Talbot W.S.; RT "A comparative map of the zebrafish genome."; RL Genome Res. 10:1903-1914(2000). RN [2] {ECO:0000313|EMBL:AAQ91271.1} RP NUCLEOTIDE SEQUENCE. RX PubMed=15520368; DOI=10.1073/pnas.0407241101; RA Song H.D., Sun X.J., Deng M., Zhang G.W., Zhou Y., Wu X.Y., Sheng Y., RA Chen Y., Ruan Z., Jiang C.L., Fan H.Y., Zon L.I., Kanki J.P., Liu T.X., RA Look A.T., Chen Z.; RT "Hematopoietic gene expression profile in zebrafish kidney marrow."; RL Proc. Natl. Acad. Sci. U.S.A. 101:16240-16245(2004). RN [3] {ECO:0000313|RefSeq:NP_997818.1} RP NUCLEOTIDE SEQUENCE. RX PubMed=15716491; DOI=10.1534/genetics.104.039727; RA Gross J.M., Perkins B.D., Amsterdam A., Egana A., Darland T., Matsui J.I., RA Sciascia S., Hopkins N., Dowling J.E.; RT "Identification of zebrafish insertional mutants with defects in visual RT system development and function."; RL Genetics 170:245-261(2005). RN [4] {ECO:0000313|RefSeq:NP_997818.1} RP NUCLEOTIDE SEQUENCE. RX PubMed=16109975; RA Woods I.G., Wilson C., Friedlander B., Chang P., Reyes D.K., Nix R., RA Kelly P.D., Chu F., Postlethwait J.H., Talbot W.S.; RT "The zebrafish gene map defines ancestral vertebrate chromosomes."; RL Genome Res. 15:1307-1314(2005). RN [5] {ECO:0000313|RefSeq:NP_997818.1} RP NUCLEOTIDE SEQUENCE. RX PubMed=18307296; DOI=10.1021/pr700667w; RA Lemeer S., Pinkse M.W., Mohammed S., van Breukelen B., den Hertog J., RA Slijper M., Heck A.J.; RT "Online automated in vivo zebrafish phosphoproteomics: from large-scale RT analysis down to a single embryo."; RL J. Proteome Res. 7:1555-1564(2008). RN [6] {ECO:0000313|Proteomes:UP000000437} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RX PubMed=23594743; DOI=10.1038/nature12111; RG Genome Reference Consortium Zebrafish; RA Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M., RA Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I., RA Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J., RA White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y., RA Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B., RA Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S., RA Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M., RA Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J., RA Clee C., Oliver K., Clark R., Riddle C., Elliot D., Eliott D., RA Threadgold G., Harden G., Ware D., Begum S., Mortimore B., Mortimer B., RA Kerry G., Heath P., Phillimore B., Tracey A., Corby N., Dunn M., RA Johnson C., Wood J., Clark S., Pelan S., Griffiths G., Smith M., RA Glithero R., Howden P., Barker N., Lloyd C., Stevens C., Harley J., RA Holt K., Panagiotidis G., Lovell J., Beasley H., Henderson C., Gordon D., RA Auger K., Wright D., Collins J., Raisen C., Dyer L., Leung K., RA Robertson L., Ambridge K., Leongamornlert D., McGuire S., Gilderthorp R., RA Griffiths C., Manthravadi D., Nichol S., Barker G., Whitehead S., Kay M., RA Brown J., Murnane C., Gray E., Humphries M., Sycamore N., Barker D., RA Saunders D., Wallis J., Babbage A., Hammond S., Mashreghi-Mohammadi M., RA Barr L., Martin S., Wray P., Ellington A., Matthews N., Ellwood M., RA Woodmansey R., Clark G., Cooper J., Cooper J., Tromans A., Grafham D., RA Skuce C., Pandian R., Andrews R., Harrison E., Kimberley A., Garnett J., RA Fosker N., Hall R., Garner P., Kelly D., Bird C., Palmer S., Gehring I., RA Berger A., Dooley C.M., Ersan-Urun Z., Eser C., Geiger H., Geisler M., RA Karotki L., Kirn A., Konantz J., Konantz M., Oberlander M., RA Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G., Osoegawa K., Zhu B., RA Rapp A., Widaa S., Langford C., Yang F., Schuster S.C., Carter N.P., RA Harrow J., Ning Z., Herrero J., Searle S.M., Enright A., Geisler R., RA Plasterk R.H., Lee C., Westerfield M., de Jong P.J., Zon L.I., RA Postlethwait J.H., Nusslein-Volhard C., Hubbard T.J., Roest Crollius H., RA Rogers J., Stemple D.L.; RT "The zebrafish reference genome sequence and its relationship to the human RT genome."; RL Nature 496:498-503(2013). RN [7] {ECO:0000313|RefSeq:NP_997818.1} RP NUCLEOTIDE SEQUENCE. RX PubMed=23637638; DOI=10.1371/journal.pgen.1003474; RA Ehrmann I., Dalgliesh C., Liu Y., Danilenko M., Crosier M., Overman L., RA Arthur H.M., Lindsay S., Clowry G.J., Venables J.P., Fort P., Elliott D.J.; RT "The tissue-specific RNA binding protein T-STAR controls regional splicing RT patterns of neurexin pre-mRNAs in the brain."; RL PLoS Genet. 9:E1003474-E1003474(2013). RN [8] {ECO:0000313|RefSeq:NP_997818.1} RP NUCLEOTIDE SEQUENCE. RX PubMed=27189481; RA Pasquier J., Cabau C., Nguyen T., Jouanno E., Severac D., Braasch I., RA Journot L., Pontarotti P., Klopp C., Postlethwait J.H., Guiguen Y., RA Bobe J.; RT "Gene evolution and gene expression after whole genome duplication in fish: RT the PhyloFish database."; RL BMC Genomics 17:368-368(2016). RN [9] {ECO:0000313|RefSeq:NP_997818.1} RP NUCLEOTIDE SEQUENCE. RX PubMed=27156127; RA Smith L.C., Clark J.C., Bisesi JH J.r., Ferguson P.L., Sabo-Attwood T.; RT "Differential recruitment of co-regulatory proteins to the human estrogen RT receptor 1 in response to xenoestrogens."; RL Comp. Biochem. Physiol. Part D Genomics Proteomics 19:159-173(2016). RN [10] {ECO:0000313|RefSeq:NP_997818.1} RP NUCLEOTIDE SEQUENCE. RX PubMed=27317806; RA Muthu V., Eachus H., Ellis P., Brown S., Placzek M.; RT "Rx3 and Shh direct anisotropic growth and specification in the zebrafish RT tuberal/anterior hypothalamus."; RL Development 143:2651-2663(2016). RN [11] {ECO:0000313|RefSeq:NP_997818.1} RP NUCLEOTIDE SEQUENCE. RX PubMed=28575219; RA Yin Y., Tang H., Liu Y., Chen Y., Li G., Liu X., Lin H.; RT "Targeted Disruption of Aromatase Reveals Dual Functions of cyp19a1a During RT Sex Differentiation in Zebrafish."; RL Endocrinology 158:3030-3041(2017). RN [12] {ECO:0000313|RefSeq:NP_997818.1} RP NUCLEOTIDE SEQUENCE. RX PubMed=30290758; RA Kim O.T.P., Nguyen P.T., Shoguchi E., Hisata K., Vo T.T.B., Inoue J., RA Shinzato C., Le B.T.N., Nishitsuji K., Kanda M., Nguyen V.H., Nong H.V., RA Satoh N.; RT "A draft genome of the striped catfish, Pangasianodon hypophthalmus, for RT comparative analysis of genes relevant to development and a resource for RT aquaculture improvement."; RL BMC Genomics 19:733-733(2018). RN [13] {ECO:0000313|RefSeq:NP_997818.1} RP IDENTIFICATION. RG RefSeq; RL Submitted (FEB-2024) to UniProtKB. CC -!- FUNCTION: Necessary for the splicing of pre-mRNA. Has a role in the CC recognition of the branch site (5'-UACUAAC-3'), the pyrimidine tract CC and the 3'-splice site at the 3'-end of introns. CC {ECO:0000256|RuleBase:RU367126}. CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000256|ARBA:ARBA00004123, CC ECO:0000256|RuleBase:RU367126}. CC -!- SIMILARITY: Belongs to the BBP/SF1 family. CC {ECO:0000256|ARBA:ARBA00010382, ECO:0000256|RuleBase:RU367126}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AY391459; AAQ91271.1; -; mRNA. DR RefSeq; NP_997818.1; NM_212653.1. DR GeneID; 572785; -. DR KEGG; dre:572785; -. DR AGR; ZFIN:ZDB-GENE-030131-2492; -. DR CTD; 7536; -. DR ZFIN; ZDB-GENE-030131-2492; sf1. DR OrthoDB; 1397at2759; -. DR Proteomes; UP000000437; Chromosome 7. DR GO; GO:0005634; C:nucleus; IBA:GO_Central. DR GO; GO:0005681; C:spliceosomal complex; IEA:UniProtKB-KW. DR GO; GO:0003729; F:mRNA binding; IBA:GO_Central. DR GO; GO:0045131; F:pre-mRNA branch point binding; IEA:UniProtKB-UniRule. DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule. DR GO; GO:0000398; P:mRNA splicing, via spliceosome; IEA:UniProtKB-UniRule. DR GO; GO:0048024; P:regulation of mRNA splicing, via spliceosome; IBA:GO_Central. DR GO; GO:0060041; P:retina development in camera-type eye; IMP:ZFIN. DR CDD; cd22382; KH-I_SF1; 1. DR Gene3D; 6.10.140.1790; -; 1. DR Gene3D; 3.30.1370.10; K Homology domain, type 1; 1. DR InterPro; IPR045071; BBP-like. DR InterPro; IPR004087; KH_dom. DR InterPro; IPR004088; KH_dom_type_1. DR InterPro; IPR036612; KH_dom_type_1_sf. DR InterPro; IPR032570; SF1-HH. DR InterPro; IPR047086; SF1-HH_sf. DR InterPro; IPR001878; Znf_CCHC. DR PANTHER; PTHR11208; RNA-BINDING PROTEIN RELATED; 1. DR PANTHER; PTHR11208:SF45; SPLICING FACTOR 1; 1. DR Pfam; PF00013; KH_1; 1. DR Pfam; PF16275; SF1-HH; 1. DR Pfam; PF00098; zf-CCHC; 1. DR PRINTS; PR01217; PRICHEXTENSN. DR SMART; SM00322; KH; 1. DR SMART; SM00343; ZnF_C2HC; 1. DR SUPFAM; SSF54791; Eukaryotic type KH-domain (KH-domain type I); 1. DR PROSITE; PS50158; ZF_CCHC; 1. PE 1: Evidence at protein level; KW Metal-binding {ECO:0000256|ARBA:ARBA00022723, KW ECO:0000256|RuleBase:RU367126}; KW mRNA processing {ECO:0000256|ARBA:ARBA00022664, KW ECO:0000256|RuleBase:RU367126}; KW mRNA splicing {ECO:0000256|ARBA:ARBA00023187, KW ECO:0000256|RuleBase:RU367126}; KW Nucleus {ECO:0000256|ARBA:ARBA00023242, ECO:0000256|RuleBase:RU367126}; KW Proteomics identification {ECO:0007829|PeptideAtlas:Q6TNQ8}; KW Reference proteome {ECO:0000313|Proteomes:UP000000437}; KW Spliceosome {ECO:0000256|RuleBase:RU367126}; KW Zinc {ECO:0000256|ARBA:ARBA00022833, ECO:0000256|RuleBase:RU367126}; KW Zinc-finger {ECO:0000256|ARBA:ARBA00022771, ECO:0000256|PROSITE- KW ProRule:PRU00047}. FT DOMAIN 279..293 FT /note="CCHC-type" FT /evidence="ECO:0000259|PROSITE:PS50158" FT REGION 1..44 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT REGION 69..90 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT REGION 328..565 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT COMPBIAS 344..364 FT /note="Pro residues" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT COMPBIAS 383..449 FT /note="Pro residues" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT COMPBIAS 450..482 FT /note="Polar residues" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT COMPBIAS 483..540 FT /note="Pro residues" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT COMPBIAS 549..565 FT /note="Pro residues" FT /evidence="ECO:0000256|SAM:MobiDB-lite" SQ SEQUENCE 565 AA; 61143 MW; DB0B14DB70AB6061 CRC64; MATGANATPL DFGQKKRKRS RWSSETPDQK TVIPGMPTVI PPGLTRDQER AYIVQLQIED LTRKLRTGDL GIPVNPEDRS PSPEPIYNSE GKRLNTREYR TRKKLEEERH SLITEMVGLN PEFKPPTDYK PPATRVSDKV MIPQDEYPEI NFVGLLIGPR GNTLKNIEKE CCAKIMIRGK GSVKEGKVGR KDGQMLPGED EPLHALVTAN TMENVKKAVE QIRYILKQGI ETPEDQNDLR KMQLRELARL NGTLREDDNR ILRPWQSTEP RSITNTTLCT KCGGAGHISS DCKFTSSFAP RPGEPPQSAQ DKARMDKEYL SLMAELGEAP VPSSGGGHNN APPSGPRPSG PNNNQPPPNR PPWMNSGPTD NRNFHGMHQG PGGPHNFPPP MPNMGGPPMP PNPNGMPPPW MQPPPPPMGQ GPAPPGHPMG PLPPWQQQAP PPPPTSSMAT SAPMPWQQNT TTTSTPATGN LPPWQQPQQT AASASQPPPP MGNPSMVPPP PGVQPPLPPG APPPPPPPPP GSAGMMYAPP PPPPPMDPNF VTMMGIPGMP PYGMPPAPPP PPPQS //