ID CHIA_RAT Reviewed; 473 AA. AC Q6RY07; DT 16-AUG-2004, integrated into UniProtKB/Swiss-Prot. DT 05-JUL-2004, sequence version 1. DT 28-JUL-2009, entry version 47. DE RecName: Full=Acidic mammalian chitinase; DE Short=AMCase; DE EC=3.2.1.14; DE Flags: Precursor; GN Name=Chia; OS Rattus norvegicus (Rat). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; OC Muroidea; Muridae; Murinae; Rattus. OX NCBI_TaxID=10116; RN [1] RP NUCLEOTIDE SEQUENCE [MRNA]. RC STRAIN=Sprague-Dawley; TISSUE=Stomach; RA Chen X.-H., Cai G.-P.; RT "Rattus norvegicus similar to acidic mammalian chitinase precursor."; RL Submitted (NOV-2003) to the EMBL/GenBank/DDBJ databases. CC -!- FUNCTION: Degrades chitin and chitotriose. May participate in the CC defense against nematodes and other pathogens (By similarity). CC -!- CATALYTIC ACTIVITY: Random hydrolysis of N-acetyl-beta-D- CC glucosaminide (1->4)-beta-linkages in chitin and chitodextrins. CC -!- SUBCELLULAR LOCATION: Secreted (By similarity). CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 18 family. Chitinase CC class II subfamily. CC -!- SIMILARITY: Contains 1 chitin-binding type-2 domain. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AY486074; AAR28968.1; -; mRNA. DR IPI; IPI00421295; -. DR RefSeq; NP_997469.1; -. DR UniGene; Rn.43692; -. DR CAZy; CBM14; Carbohydrate-Binding Module Family 14. DR CAZy; GH18; Glycoside Hydrolase Family 18. DR Ensembl; ENSRNOT00000039871; ENSRNOP00000043368; ENSRNOG00000033162; Rattus norvegicus. DR Ensembl; ENSRNOT00000041562; ENSRNOP00000044947; ENSRNOG00000033162; Rattus norvegicus. DR GeneID; 113901; -. DR KEGG; rno:113901; -. DR RGD; 1303058; Chia. DR HOVERGEN; Q6RY07; -. DR BRENDA; 3.2.1.14; 248. DR NextBio; 617982; -. DR ArrayExpress; Q6RY07; -. DR GermOnline; ENSRNOG00000033162; Rattus norvegicus. DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell. DR GO; GO:0043169; F:cation binding; IEA:InterPro. DR GO; GO:0008061; F:chitin binding; IEA:UniProtKB-KW. DR GO; GO:0004568; F:chitinase activity; IEA:EC. DR GO; GO:0006032; P:chitin catabolic process; IEA:UniProtKB-KW. DR GO; GO:0000272; P:polysaccharide catabolic process; IEA:UniProtKB-KW. DR InterPro; IPR002557; Chitin-bd_peritrophin-A. DR InterPro; IPR011583; Chitinase_II. DR InterPro; IPR001223; Glyco_hydro18cat. DR InterPro; IPR001579; Glyco_hydro_18_chit_AS. DR InterPro; IPR013781; Glyco_hydro_sg_catalytic. DR Gene3D; G3DSA:3.20.20.80; Glyco_hydro_cat; 1. DR Pfam; PF01607; CBM_14; 1. DR Pfam; PF00704; Glyco_hydro_18; 1. DR ProDom; PD000471; Chitinase_II; 1. DR SMART; SM00494; ChtBD2; 1. DR SMART; SM00636; Glyco_18; 1. DR PROSITE; PS50940; CHIT_BIND_II; 1. DR PROSITE; PS01095; CHITINASE_18; 1. PE 2: Evidence at transcript level; KW Carbohydrate metabolism; Chitin degradation; Chitin-binding; KW Disulfide bond; Glycosidase; Hydrolase; Polysaccharide degradation; KW Secreted; Signal. FT SIGNAL 1 21 By similarity. FT CHAIN 22 473 Acidic mammalian chitinase. FT /FTId=PRO_0000011946. FT DOMAIN 424 473 Chitin-binding type-2. FT COMPBIAS 409 425 Gly/Ser-rich. FT ACT_SITE 140 140 Proton donor (By similarity). FT DISULFID 26 51 By similarity. FT DISULFID 307 372 By similarity. FT DISULFID 457 470 By similarity. SQ SEQUENCE 473 AA; 51951 MW; FBB0DB91A42C1EFD CRC64; MAKLILVTGL VLLLNVQLGS AYNLVCYFTN WAQYRPGLGS FKPDDINPCL CTHLIYAFAG MQNNQITTIE WNDVTLYKAF NDLKNRNSKL KTLLAIGGWN FGTAPFTTMV STSQNRQTFI TSVIKFLRQY GFDGLDLDWE YPGSRGSPPQ DKHLFTVLVK ELREAFEQEA IESNRPRLMV TAAVAAGISN IQAGYEIPEL SQYLDFIHVM TYDLHGSWDG YTGENSPLYK LPTETGSNAY LNVDYVMNYW KDNGAPAEKL IVGFPEYGHT YILSNPSDTG IGAPTSGNGP AGPYTRQAGF WAYYEICTFL RNGATQDWDA PQEVPYAYKG NEWVGYDNIK SFSVKAQWLK QNNFGGAMIW AIDLDDFTGS FCDQGKFPLT STLNKALDIP TAGCTAPDLP SEPVTTPPGS GSGGGSSGGG SEGSGFCAGK ADGLYPVADD RNAFWHCING ITYQQHCQAG LVFDTSCNCC NWP //