ID   S4A5_RAT                Reviewed;        1112 AA.
AC   Q6RI88;
DT   08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   07-SEP-2016, entry version 87.
DE   RecName: Full=Electrogenic sodium bicarbonate cotransporter 4;
DE   AltName: Full=Solute carrier family 4 member 5;
GN   Name=Slc4a5 {ECO:0000312|RGD:1303009};
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi;
OC   Muroidea; Muridae; Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1] {ECO:0000305, ECO:0000312|EMBL:AAS98674.1}
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, AND TISSUE
RP   SPECIFICITY.
RC   STRAIN=Sprague-Dawley {ECO:0000312|EMBL:AAS98674.1};
RC   TISSUE=Liver {ECO:0000269|PubMed:15151908};
RX   PubMed=15151908; DOI=10.1152/ajpcell.00590.2003;
RA   Abuladze N., Pushkin A., Tatishchev S., Newman D., Sassani P.,
RA   Kurtz I.;
RT   "Expression and localization of rat NBC4c in liver and renal
RT   uroepithelium.";
RL   Am. J. Physiol. 287:C781-C789(2004).
RN   [2] {ECO:0000305}
RP   TISSUE SPECIFICITY.
RX   PubMed=12388414;
RA   Xu J., Wang Z., Barone S., Petrovic M., Amlal H., Conforti L.,
RA   Petrovic S., Soleimani M.;
RT   "Expression of the Na+-HCO-3 cotransporter NBC4 in rat kidney and
RT   characterization of a novel NBC4 variant.";
RL   Am. J. Physiol. 284:F41-F50(2003).
CC   -!- FUNCTION: Mediates sodium- and bicarbonate-dependent electrogenic
CC       sodium bicarbonate cotransport, with a Na(+):HCO3(-) stoichiometry
CC       of 2:1. May have a housekeeping function in regulating the pH of
CC       tissues in which it is expressed. May play a role in mediating
CC       Na(+):HCO3(-) cotransport in hepatocytes and intrahepatic
CC       cholangiocytes. Also may be important in protecting the renal
CC       paranchyma from alterations in urine pH.
CC       {ECO:0000250|UniProtKB:Q9BY07, ECO:0000269|PubMed:15151908}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:15151908};
CC       Multi-pass membrane protein {ECO:0000269|PubMed:15151908}.
CC       Basolateral cell membrane {ECO:0000269|PubMed:15151908}; Multi-
CC       pass membrane protein {ECO:0000269|PubMed:15151908}.
CC       Note=Localised predominantly to the basolateral sinusoidal
CC       membrane in hepatocytes. {ECO:0000269|PubMed:15151908}.
CC   -!- TISSUE SPECIFICITY: Observed in hepatocytes and in the apical
CC       region of bile duct intrahepatic cholangiocytes of liver. Also
CC       observed in uroepithelium cells lining the outer pelvic wall of
CC       the kidney (at protein level). Highly expressed in colon, distal
CC       colon, liver, kidney and testis. Moderate expression in duodenum
CC       and stomach and weak expression in heart. In kidney, very weakly
CC       expressed in the inner medulla, but abundantly expressed in cortex
CC       and outer medulla in the medullary thick ascending and cortical
CC       thick ascending limbs of the loop of Henle.
CC       {ECO:0000269|PubMed:12388414, ECO:0000269|PubMed:15151908}.
CC   -!- SIMILARITY: Belongs to the anion exchanger (TC 2.A.31) family.
CC       {ECO:0000255}.
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DR   EMBL; AY496959; AAS98674.1; -; mRNA.
DR   RefSeq; NP_997677.1; NM_212512.1.
DR   UniGene; Rn.214577; -.
DR   ProteinModelPortal; Q6RI88; -.
DR   STRING; 10116.ENSRNOP00000014249; -.
DR   PaxDb; Q6RI88; -.
DR   PRIDE; Q6RI88; -.
DR   Ensembl; ENSRNOT00000014249; ENSRNOP00000014249; ENSRNOG00000010378.
DR   GeneID; 297386; -.
DR   KEGG; rno:297386; -.
DR   UCSC; RGD:1303009; rat.
DR   CTD; 57835; -.
DR   RGD; 1303009; Slc4a5.
DR   eggNOG; KOG1172; Eukaryota.
DR   eggNOG; ENOG410XPHD; LUCA.
DR   GeneTree; ENSGT00760000119021; -.
DR   HOGENOM; HOG000280684; -.
DR   HOVERGEN; HBG004326; -.
DR   InParanoid; Q6RI88; -.
DR   KO; K13857; -.
DR   OMA; ELIWTGR; -.
DR   OrthoDB; EOG091G01FT; -.
DR   PhylomeDB; Q6RI88; -.
DR   TreeFam; TF313630; -.
DR   Reactome; R-RNO-425381; Bicarbonate transporters.
DR   PRO; PR:Q6RI88; -.
DR   Proteomes; UP000002494; Chromosome 4.
DR   Bgee; ENSRNOG00000010378; -.
DR   ExpressionAtlas; Q6RI88; baseline and differential.
DR   GO; GO:0016323; C:basolateral plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; TAS:RGD.
DR   GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR   GO; GO:0015301; F:anion:anion antiporter activity; IEA:UniProtKB-KW.
DR   GO; GO:0005452; F:inorganic anion exchanger activity; TAS:RGD.
DR   GO; GO:0006820; P:anion transport; TAS:RGD.
DR   GO; GO:0015701; P:bicarbonate transport; IBA:GO_Central.
DR   GO; GO:0033326; P:cerebrospinal fluid secretion; IEA:Ensembl.
DR   GO; GO:0002064; P:epithelial cell development; IEA:Ensembl.
DR   GO; GO:0048311; P:mitochondrion distribution; IEA:Ensembl.
DR   GO; GO:0010468; P:regulation of gene expression; IEA:Ensembl.
DR   GO; GO:0051453; P:regulation of intracellular pH; IBA:GO_Central.
DR   GO; GO:0006885; P:regulation of pH; TAS:RGD.
DR   GO; GO:0003073; P:regulation of systemic arterial blood pressure; IEA:Ensembl.
DR   GO; GO:0003014; P:renal system process; IBA:GO_Central.
DR   GO; GO:0060041; P:retina development in camera-type eye; IEA:Ensembl.
DR   GO; GO:0006814; P:sodium ion transport; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.1100.10; -; 1.
DR   InterPro; IPR013769; Band3_cytoplasmic_dom.
DR   InterPro; IPR011531; HCO3_transpt_C.
DR   InterPro; IPR003020; HCO3_transpt_euk.
DR   InterPro; IPR003024; Na/HCO3_transpt.
DR   InterPro; IPR016152; PTrfase/Anion_transptr.
DR   PANTHER; PTHR11453; PTHR11453; 2.
DR   Pfam; PF07565; Band_3_cyto; 1.
DR   Pfam; PF00955; HCO3_cotransp; 1.
DR   PRINTS; PR01231; HCO3TRNSPORT.
DR   PRINTS; PR01232; NAHCO3TRSPRT.
DR   SUPFAM; SSF55804; SSF55804; 1.
DR   TIGRFAMs; TIGR00834; ae; 1.
PE   1: Evidence at protein level;
KW   Anion exchange; Cell membrane; Complete proteome; Disulfide bond;
KW   Ion transport; Membrane; Reference proteome; Sodium; Sodium transport;
KW   Transmembrane; Transmembrane helix; Transport.
FT   CHAIN         1   1112       Electrogenic sodium bicarbonate
FT                                cotransporter 4.
FT                                /FTId=PRO_0000328921.
FT   TOPO_DOM      1    513       Cytoplasmic. {ECO:0000255}.
FT   TRANSMEM    514    536       Helical. {ECO:0000255}.
FT   TOPO_DOM    537    547       Extracellular. {ECO:0000255}.
FT   TRANSMEM    548    579       Helical. {ECO:0000255}.
FT   TOPO_DOM    580    598       Cytoplasmic. {ECO:0000255}.
FT   TRANSMEM    599    620       Helical. {ECO:0000255}.
FT   TOPO_DOM    621    734       Extracellular. {ECO:0000255}.
FT   TRANSMEM    735    753       Helical. {ECO:0000255}.
FT   TOPO_DOM    754    772       Cytoplasmic. {ECO:0000255}.
FT   TRANSMEM    773    792       Helical. {ECO:0000255}.
FT   TOPO_DOM    793    820       Extracellular. {ECO:0000255}.
FT   TRANSMEM    821    839       Helical. {ECO:0000255}.
FT   TOPO_DOM    840    858       Cytoplasmic. {ECO:0000255}.
FT   TRANSMEM    859    875       Helical. {ECO:0000255}.
FT   TOPO_DOM    876    880       Extracellular. {ECO:0000255}.
FT   TRANSMEM    881    900       Helical. {ECO:0000255}.
FT   TOPO_DOM    901    920       Cytoplasmic. {ECO:0000255}.
FT   TRANSMEM    921    940       Helical. {ECO:0000255}.
FT   TOPO_DOM    941    945       Extracellular. {ECO:0000255}.
FT   TRANSMEM    946    966       Helical. {ECO:0000255}.
FT   TOPO_DOM    967    992       Cytoplasmic. {ECO:0000255}.
FT   TRANSMEM    993   1010       Helical. {ECO:0000255}.
FT   TOPO_DOM   1011   1015       Extracellular. {ECO:0000255}.
FT   TRANSMEM   1016   1033       Helical. {ECO:0000255}.
FT   TOPO_DOM   1034   1112       Cytoplasmic. {ECO:0000255}.
FT   COMPBIAS    432    465       Gly-rich. {ECO:0000255}.
FT   DISULFID    671    673       {ECO:0000250|UniProtKB:Q9Y6R1}.
FT   DISULFID    717    729       {ECO:0000250|UniProtKB:Q9Y6R1}.
SQ   SEQUENCE   1112 AA;  123944 MW;  BE5EF90A543DA35C CRC64;
     MKVEEKAGVK KLEPTSYRRR HPEQDFPSIH IGFPVPGYSQ RKSDSKGHLS GLQRVQWSLQ
     PDKSQQDLAG PDGIKASSLG GSVDFTKRIR SPAAEQLQDI LGEEDEAPNP TLFTEMDTLQ
     HDGDQMEWKE SARWIKFEEK VEEGGERWSK PHVSTLSLHS LFELRTCLQT GTVLLDLDSG
     SLPQIIDDVI EKQIEGGLLR PELRERVSYV LLRKHRHQTK KPIHRSLADI GKSVSTTNRS
     SARSPSAGPT LHHSTEDLRI RQSTSYGHLC HAQSRSMNDI SHTPNTDQRK NKFMKKIPKD
     SEASNVLVGE VDFLDQPFIA FVRLAQSSML GGVTEVPVPT RFLFILLGPS GRAKSYNEIG
     RAIATLMVDD LFSDVAYKAR NREDLIAGVD EFLDEVIVLP PGEWDPNIRI EPPKKVPSAD
     KRKSVFSLAE LGQMNGSVGR SGASAGGGGS GGGAGGSGAG GGGSGNEAEM PAMHEIGEEL
     IWTGRFFGGL RLDVKRKLPW FPSDFYDGFH IQSISAVLFI YLGCITNAIT FGGLLGDATD
     NYQGVMESFL GTAMAGSLFC LFSGQPLIIL SSTGPILIFE KLLFDFSKAN GLDYMEFRLW
     IGLHSAIQCL ILVATDASFI IKYITRFTEE GFSTLISFIF IYDAIKKMIG AFKYYPINTD
     FKPDSITTYK CECVAPDTVN TTTVNDSALL VPNTNMSVYT PLNLTALDWS LLSKKECLSY
     GGRLLGSSCQ FVPDLALMSF ILFFGTYSMT LTLKKFKFSR YFPTKVRTLV ADFSIVFSIL
     LFCGIDACFG LQTPKLHVPN VIKPTRPDRG WFVAPFGKNP WWVYPASILP ALLVTILIFM
     DQQITAVIVN RKENKLRKAA GYHLDLFWVG ILMALCSFMG LPWYVAATVI SIAHIDSLKM
     ETETSAPGEQ PQFLGVREQR VTGVMVFILT GISVFLAPIL KYIPMPVLYG VFLYMGVASL
     NGIQFWDRCK LFLMPAKHQP DHAFLRHVPL RRIHLFTLVQ ILCLALLWIL KSTMAAIIFP
     VMILGLIIVR RLLDLIFSQH DLAWIDNILP EKEKKESDRK KRRKEVHENT DKEPQFLPPS
     VVKIPMEGIP SDPQNGIHCV GRKRSSSWSH SL
//