ID Q6P8N8_MOUSE Unreviewed; 620 AA. AC Q6P8N8; DT 05-JUL-2004, integrated into UniProtKB/TrEMBL. DT 05-JUL-2004, sequence version 1. DT 03-AUG-2022, entry version 146. DE SubName: Full=ATP-dependent Clp protease ATP-binding subunit clpX-like, mitochondrial {ECO:0000313|Ensembl:ENSMUSP00000109455}; DE SubName: Full=Caseinolytic peptidase X (E.coli) {ECO:0000313|EMBL:AAH61153.1}; GN Name=Clpx {ECO:0000313|EMBL:AAH61153.1, GN ECO:0000313|Ensembl:ENSMUSP00000109455, ECO:0000313|MGI:MGI:1346017}; OS Mus musculus (Mouse). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; OC Murinae; Mus; Mus. OX NCBI_TaxID=10090 {ECO:0000313|EMBL:AAH61153.1}; RN [1] {ECO:0000313|EMBL:AAH61153.1} RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RC TISSUE=Brain {ECO:0000313|EMBL:AAH61153.1}; RX PubMed=15489334; DOI=10.1101/gr.2596504; RG The MGC Project Team; RA Gerhard D.S., Wagner L., Feingold E.A., Shenmen C.M., Grouse L.H., RA Schuler G., Klein S.L., Old S., Rasooly R., Good P., Guyer M., Peck A.M., RA Derge J.G., Lipman D., Collins F.S., Jang W., Sherry S., Feolo M., RA Misquitta L., Lee E., Rotmistrovsky K., Greenhut S.F., Schaefer C.F., RA Buetow K., Bonner T.I., Haussler D., Kent J., Kiekhaus M., Furey T., RA Brent M., Prange C., Schreiber K., Shapiro N., Bhat N.K., Hopkins R.F., RA Hsie F., Driscoll T., Soares M.B., Casavant T.L., Scheetz T.E., RA Brown-stein M.J., Usdin T.B., Toshiyuki S., Carninci P., Piao Y., RA Dudekula D.B., Ko M.S., Kawakami K., Suzuki Y., Sugano S., Gruber C.E., RA Smith M.R., Simmons B., Moore T., Waterman R., Johnson S.L., Ruan Y., RA Wei C.L., Mathavan S., Gunaratne P.H., Wu J., Garcia A.M., Hulyk S.W., RA Fuh E., Yuan Y., Sneed A., Kowis C., Hodgson A., Muzny D.M., McPherson J., RA Gibbs R.A., Fahey J., Helton E., Ketteman M., Madan A., Rodrigues S., RA Sanchez A., Whiting M., Madari A., Young A.C., Wetherby K.D., Granite S.J., RA Kwong P.N., Brinkley C.P., Pearson R.L., Bouffard G.G., Blakesly R.W., RA Green E.D., Dickson M.C., Rodriguez A.C., Grimwood J., Schmutz J., RA Myers R.M., Butterfield Y.S., Griffith M., Griffith O.L., Krzywinski M.I., RA Liao N., Morin R., Morrin R., Palmquist D., Petrescu A.S., Skalska U., RA Smailus D.E., Stott J.M., Schnerch A., Schein J.E., Jones S.J., Holt R.A., RA Baross A., Marra M.A., Clifton S., Makowski K.A., Bosak S., Malek J.; RT "The status, quality, and expansion of the NIH full-length cDNA project: RT the Mammalian Gene Collection (MGC)."; RL Genome Res. 14:2121-2127(2004). RN [2] {ECO:0000313|Ensembl:ENSMUSP00000109455, ECO:0000313|Proteomes:UP000000589} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=C57BL/6J {ECO:0000313|Ensembl:ENSMUSP00000109455, RC ECO:0000313|Proteomes:UP000000589}; RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112; RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S., RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., RA Eichler E.E., Ponting C.P.; RT "Lineage-specific biology revealed by a finished genome assembly of the RT mouse."; RL PLoS Biol. 7:E1000112-E1000112(2009). RN [3] {ECO:0007829|PubMed:21183079} RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001; RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R., RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.; RT "A tissue-specific atlas of mouse protein phosphorylation and expression."; RL Cell 143:1174-1189(2010). RN [4] {ECO:0000313|Ensembl:ENSMUSP00000109455} RP IDENTIFICATION. RC STRAIN=C57BL/6J {ECO:0000313|Ensembl:ENSMUSP00000109455}; RG Ensembl; RL Submitted (FEB-2012) to UniProtKB. CC -!- SIMILARITY: Belongs to the ClpX chaperone family. {ECO:0000256|PROSITE- CC ProRule:PRU01250}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; BC061153; AAH61153.1; -; mRNA. DR RefSeq; NP_001037854.1; NM_001044389.2. DR SMR; Q6P8N8; -. DR MaxQB; Q6P8N8; -. DR PRIDE; Q6P8N8; -. DR ProteomicsDB; 332574; -. DR Antibodypedia; 51984; 99 antibodies from 25 providers. DR DNASU; 270166; -. DR Ensembl; ENSMUST00000113824.8; ENSMUSP00000109455.2; ENSMUSG00000015357.11. DR GeneID; 270166; -. DR UCSC; uc009qdc.2; mouse. DR CTD; 10845; -. DR MGI; MGI:1346017; Clpx. DR VEuPathDB; HostDB:ENSMUSG00000015357; -. DR GeneTree; ENSGT00390000017625; -. DR HOGENOM; CLU_014218_0_1_1; -. DR BioGRID-ORCS; 270166; 11 hits in 72 CRISPR screens. DR ChiTaRS; Clpx; mouse. DR Proteomes; UP000000589; Chromosome 9. DR Bgee; ENSMUSG00000015357; Expressed in spermatid and 241 other tissues. DR ExpressionAtlas; Q6P8N8; baseline and differential. DR GO; GO:0005524; F:ATP binding; IEA:InterPro. DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro. DR GO; GO:0046983; F:protein dimerization activity; IEA:UniProtKB-UniRule. DR GO; GO:0051082; F:unfolded protein binding; IEA:UniProtKB-UniRule. DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule. DR GO; GO:0006457; P:protein folding; IEA:UniProtKB-UniRule. DR Gene3D; 3.40.50.300; -; 1. DR InterPro; IPR003593; AAA+_ATPase. DR InterPro; IPR003959; ATPase_AAA_core. DR InterPro; IPR019489; Clp_ATPase_C. DR InterPro; IPR027417; P-loop_NTPase. DR InterPro; IPR010603; Znf_CppX_C4. DR Pfam; PF07724; AAA_2; 1. DR Pfam; PF10431; ClpB_D2-small; 1. DR SMART; SM00382; AAA; 1. DR SMART; SM01086; ClpB_D2-small; 1. DR SUPFAM; SSF52540; SSF52540; 1. DR PROSITE; PS51902; CLPX_ZB; 1. PE 1: Evidence at protein level; KW Chaperone {ECO:0000256|PROSITE-ProRule:PRU01250}; KW Metal-binding {ECO:0000256|ARBA:ARBA00022723, ECO:0000256|PROSITE- KW ProRule:PRU01250}; KW Proteomics identification {ECO:0007829|EPD:Q6P8N8, KW ECO:0007829|MaxQB:Q6P8N8}; KW Reference proteome {ECO:0000313|Proteomes:UP000000589}; KW Zinc {ECO:0000256|ARBA:ARBA00022833, ECO:0000256|PROSITE-ProRule:PRU01250}. FT DOMAIN 94..147 FT /note="ClpX-type ZB" FT /evidence="ECO:0000259|PROSITE:PS51902" FT REGION 69..102 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT REGION 585..620 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT COMPBIAS 81..101 FT /note="Polar residues" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT COMPBIAS 585..600 FT /note="Basic and acidic residues" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT BINDING 106 FT /ligand="Zn(2+)" FT /ligand_id="ChEBI:CHEBI:29105" FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01250" FT BINDING 109 FT /ligand="Zn(2+)" FT /ligand_id="ChEBI:CHEBI:29105" FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01250" FT BINDING 128 FT /ligand="Zn(2+)" FT /ligand_id="ChEBI:CHEBI:29105" FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01250" FT BINDING 131 FT /ligand="Zn(2+)" FT /ligand_id="ChEBI:CHEBI:29105" FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01250" SQ SEQUENCE 620 AA; 67336 MW; 44D82B9F5AE6F81C CRC64; MSSCGACTCG AAAARLLTTS LTSAQRGISC GRIHVPVLGR LGTTLDAQAL RRAPLRTFSE TPAYFASKDG ANKDGSGDGN KKSVTEGSSK KSGSGNSGKG GNQLRCPKCG DLCTHVETFV SSTRFVKCEK CHHFFVVLSE ADSKKSIIKE PESAAEAVKL AFQQKPPPPP KKIYNYLDKY VVGQSFAKKV LSVAVYNHYK RIYNNIPANL RQQAEAEKQT SLTPRELLQI AGISPHGNAL GASMQQQVNQ QMPQEKRGGE VLDSSQDDIK LEKSNILLLG PTGSGKTLLA QTLAKCLDVP FAICDCTTLT QAGYVGEDIE SVIAKLLQDA NYNVEKAQQG IVFLDEVDKI GSVPGIHQLR DVGGEGVQQG LLKLLEGTIV NVPEKNSRKL RGETVQVDTT NVLFVASGAF NGLDRIISRR KNEKYLGFGT PSNLGKGRRA AAAADLANRS GESNTHQDIE EKDRLLRHVE ARDLIEFGMI PEFVGRLPVV VPLHSLDEKT LVQILTEPRN AVIPQYQALF SMDKCELNVT EDALKAIARL ALERKTGARG LRSIMEKLLL EPMFEVPNSD IVCVEVDKEV VEGKKEPGYI RAPSKESSEE EYDSGVEEDG WPRQADAANS //