ID HLDE_RHOPA STANDARD; PRT; 490 AA. AC Q6N2R5; DT 25-OCT-2004 (Rel. 45, Created) DT 25-OCT-2004 (Rel. 45, Last sequence update) DT 10-MAY-2005 (Rel. 47, Last annotation update) DE Bifunctional protein hldE [Includes: D-beta-D-heptose 7-phosphate DE kinase (EC 2.7.1.-) (D-beta-D-heptose 7-phosphotransferase); D-beta-D- DE heptose 1-phosphate adenosyltransferase (EC 2.7.7.-)]. GN Name=hldE; Synonyms=rfaE; OrderedLocusNames=RPA3984; OS Rhodopseudomonas palustris. OC Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales; OC Bradyrhizobiaceae; Rhodopseudomonas. OX NCBI_TaxID=1076; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=CGA009 / ATCC BAA-98; RX PubMed=14704707; DOI=10.1038/nbt923; RA Larimer F.W., Chain P., Hauser L., Lamerdin J.E., Malfatti S., Do L., RA Land M.L., Pelletier D.A., Beatty J.T., Lang A.S., Tabita F.R., RA Gibson J.L., Hanson T.E., Bobst C., Torres y Torres J.L., Peres C., RA Harrison F.H., Gibson J., Harwood C.S.; RT "Complete genome sequence of the metabolically versatile RT photosynthetic bacterium Rhodopseudomonas palustris."; RL Nat. Biotechnol. 22:55-61(2004). CC -!- FUNCTION: Catalyzes the phosphorylation of D-glycero-D-manno- CC heptose 7-phosphate at the C-1 position to form D,D-heptose-1,7- CC bisphosphate (By similarity). CC -!- FUNCTION: Catalyzes the ADP transfer to D-glycero-D-manno-heptose CC 1-phosphate, yielding ADP-D,D-heptose (By similarity). CC -!- CATALYTIC ACTIVITY: ATP + D-glycero-beta-D-manno-heptose 7- CC phosphate = ADP + D-glycero-beta-D-manno-heptose 1,7-bisphosphate. CC -!- CATALYTIC ACTIVITY: ATP + D-glycero-beta-D-manno-heptose 1- CC phosphate = diphosphate + ADP-D-glycero-beta-D-manno-heptose. CC -!- PATHWAY: ADP-L-glycero-beta-D-manno-heptose biosynthesis; second CC step. CC -!- PATHWAY: ADP-L-glycero-beta-D-manno-heptose biosynthesis; fourth CC step. CC -!- SIMILARITY: In the N-terminal section; belongs to the carbohydrate CC kinase pfkB family. CC -!- SIMILARITY: In the C-terminal section; belongs to the CC cytidylyltransferase family. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; BX572605; CAE29425.1; -; Genomic_DNA. DR HAMAP; MF_01603; -; 1. DR InterPro; IPR004821; Cyt_trans_rel. DR InterPro; IPR004820; Cytidylyltransf. DR InterPro; IPR002173; PfkB. DR InterPro; IPR011611; PfkB_region. DR InterPro; IPR011913; RfaE_dom_I. DR InterPro; IPR011914; RfaE_dom_II. DR Pfam; PF01467; CTP_transf_2; 1. DR Pfam; PF00294; PfkB; 1. DR TIGRFAMs; TIGR00125; cyt_tran_rel; 1. DR TIGRFAMs; TIGR02198; rfaE_dom_I; 1. DR TIGRFAMs; TIGR02199; rfaE_dom_II; 1. DR PROSITE; PS00583; PFKB_KINASES_1; 1. DR PROSITE; PS00584; PFKB_KINASES_2; FALSE_NEG. KW Carbohydrate metabolism; Complete proteome; Kinase; KW Multifunctional enzyme; Nucleotidyltransferase; Transferase. FT REGION 1 330 Ribokinase. FT REGION 358 490 Cytidylyltransferase. SQ SEQUENCE 490 AA; 52131 MW; E9E281E226F4FF60 CRC64; MFSFDALLQA IARQTVLCVG DLMLDEFVYG EVSRISPEAP APVIAVQRSE TNIGGAGNVA RNIAAIGARC IFVGLIGDDA TGRFLESELG SESRIEPVLV CDGSRPTTRK VRFVSEHFST HMLRADWETA SPAAADIEQR LLDAILPQLA RADIVLLSDY AKGVLTARVI RDTIDAAKKL GKRVIVDPKS ANFAIYRGAT LLTPNRKEFT AATRSAAATD DEIAAAAQDA MALAECEAML VTKSEHGMTL VPRGGEPIHV PALPVKVRDV SGAGDTVAAV LAVVLASGAN WATAMRAASA AAAVAVSKNG TAVVTPAELR RRILPHASLA AEEKIIGSDE ELDERLKQWR REGLRVGFTN GCFDILHPGH VKVLTAARGA CDRLIVGLNS DASVRRLKGE SRPVQHERAR AEVLAALEAV DLVAIFEEDT PLRLITRIEP SVLVKGGDYT REQVVGHEIV AAKGGDVLLV DVLPGFSTTS LVARAREGQS //