ID DOIAD_STRKN Reviewed; 343 AA. AC Q6L743; DT 02-MAY-2006, integrated into UniProtKB/Swiss-Prot. DT 05-JUL-2004, sequence version 1. DT 02-SEP-2008, entry version 22. DE RecName: Full=2-deoxy-scyllo-inosamine dehydrogenase; DE Short=DOIA dehydrogenase; DE EC=1.1.1.-; GN Name=kanE; Synonyms=kanK; OS Streptomyces kanamyceticus. OC Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales; OC Streptomycineae; Streptomycetaceae; Streptomyces. OX NCBI_TaxID=1967; RN [1] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RC STRAIN=ATCC 12853 / DSM 40500 / IFO 13414 / NCIMB 9343 / NRRL B-2535; RX PubMed=15313224; DOI=10.1016/j.abb.2004.06.009; RA Kharel M.K., Subba B., Basnet D.B., Woo J.S., Lee H.C., Liou K., RA Sohng J.K.; RT "A gene cluster for biosynthesis of kanamycin from Streptomyces RT kanamyceticus: comparison with gentamicin biosynthetic gene cluster."; RL Arch. Biochem. Biophys. 429:204-214(2004). RN [2] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RC STRAIN=21-18; RX PubMed=15303497; RA Yanai K., Murakami T.; RT "The kanamycin biosynthetic gene cluster from Streptomyces RT kanamyceticus."; RL J. Antibiot. 57:351-354(2004). RN [3] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RC STRAIN=ATCC 12853 / DSM 40500 / IFO 13414 / NCIMB 9343 / NRRL B-2535; RA Aboshanab K.M.A., Schmidt-Beissner H., Wehmeier U.F., Welzel K., RA Vente A., Piepersberg W.; RT "Cloning and sequencing of the kanamycin biosynthetic gene cluster RT from Streptomyces kanamyceticus DSM 40500."; RL Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases. CC -!- FUNCTION: Catalyzes the oxidation of 2-deoxy-scyllo-inosamine CC (DOIA) with NAD(+) or NADP(+), forming 3-amino-2,3-dideoxy-scyllo- CC inosose (amino-DOI) (By similarity). CC -!- CATALYTIC ACTIVITY: 1-amino-1,2-dideoxy-scyllo-inositol + CC NAD(P)(+) = 5-amino-2,3,4-trihydroxycyclohexanone + NAD(P)H. CC -!- COFACTOR: Binds 2 zinc ions per subunit (By similarity). CC -!- PATHWAY: Metabolic intermediate biosynthesis; 2-deoxystreptamine CC biosynthesis; 2-deoxystreptamine from D-glucose-6-phosphate: step CC 3/4. CC -!- PATHWAY: Context: Kanamycin biosynthesis. CC -!- SIMILARITY: Belongs to the zinc-containing alcohol dehydrogenase CC family. DOIA dehydrogenase subfamily. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AJ582817; CAF60530.1; -; Genomic_DNA. DR EMBL; AB164642; BAD20754.1; -; Genomic_DNA. DR EMBL; AJ628422; CAF31584.1; -; Genomic_DNA. DR InterPro; IPR013154; AlcDHase_GroES-like. DR InterPro; IPR002085; AlcDHase_SF_Zn. DR InterPro; IPR013149; AlcDHase_Zn-bd. DR InterPro; IPR002328; AlcDHase_Zn_CS. DR PANTHER; PTHR11695; ADH_Sf_Zn; 1. DR Pfam; PF08240; ADH_N; 1. DR Pfam; PF00107; ADH_zinc_N; 1. DR PROSITE; PS00059; ADH_ZINC; FALSE_NEG. PE 3: Inferred from homology; KW Metal-binding; NAD; NADP; Oxidoreductase; Zinc. FT CHAIN 1 343 2-deoxy-scyllo-inosamine dehydrogenase. FT /FTId=PRO_0000234044. FT METAL 37 37 Zinc 1; catalytic (By similarity). FT METAL 59 59 Zinc 1; catalytic (By similarity). FT METAL 91 91 Zinc 2 (By similarity). FT METAL 94 94 Zinc 2 (By similarity). FT METAL 97 97 Zinc 2 (By similarity). FT METAL 105 105 Zinc 2 (By similarity). FT METAL 146 146 Zinc 1; catalytic (By similarity). SQ SEQUENCE 343 AA; 35936 MW; A61AFA1521BAEFB8 CRC64; MKALVFHSPE KATFEQRDVP TPRPGEALVH IAYNSICGSD LSLYRGVWHG FGYPVVPGHE WSGTVVEING ANGHDQSLVG KNVVGDLTCA CGNCAACGRG TPVLCENLQE LGFTKDGACA EYMTIPVDNL RPLPDALSLR SACQVEPLAV ALNAVSIAGV APGDRVAVMG AGGIGLMLMQ VARHLGGEVT VVSEPVAERR AVAGQLGATE LCSAEPGQLA ELVARRPELT PDVVLEASGY PAALQEAIEV VRPGGRIGLI GYRVEETGPM SPQHIAVKAL TLRGSLGPGG RFDDAVELLA KGDDIAVEPL LSHEFGLADY ATALDLALSR TNGNVRSFFN LRD //