ID NUP85_XENTR Reviewed; 653 AA. AC Q6DK84; DT 18-MAR-2008, integrated into UniProtKB/Swiss-Prot. DT 16-AUG-2004, sequence version 1. DT 02-OCT-2024, entry version 105. DE RecName: Full=Nuclear pore complex protein Nup85; DE AltName: Full=85 kDa nucleoporin; DE AltName: Full=Nucleoporin Nup85; GN Name=nup85; OS Xenopus tropicalis (Western clawed frog) (Silurana tropicalis). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia; OC Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Silurana. OX NCBI_TaxID=8364; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RC TISSUE=Embryo; RG NIH - Xenopus Gene Collection (XGC) project; RL Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases. RN [2] RP FUNCTION, AND DISRUPTION PHENOTYPE. RX PubMed=30179222; DOI=10.1172/jci98688; RA Braun D.A., Lovric S., Schapiro D., Schneider R., Marquez J., Asif M., RA Hussain M.S., Daga A., Widmeier E., Rao J., Ashraf S., Tan W., Lusk C.P., RA Kolb A., Jobst-Schwan T., Schmidt J.M., Hoogstraten C.A., Eddy K., RA Kitzler T.M., Shril S., Moawia A., Schrage K., Khayyat A.I.A., Lawson J.A., RA Gee H.Y., Warejko J.K., Hermle T., Majmundar A.J., Hugo H., Budde B., RA Motameny S., Altmueller J., Noegel A.A., Fathy H.M., Gale D.P., RA Waseem S.S., Khan A., Kerecuk L., Hashmi S., Mohebbi N., Ettenger R., RA Serdaroglu E., Alhasan K.A., Hashem M., Goncalves S., Ariceta G., RA Ubetagoyena M., Antonin W., Baig S.M., Alkuraya F.S., Shen Q., Xu H., RA Antignac C., Lifton R.P., Mane S., Nuernberg P., Khokha M.K., RA Hildebrandt F.; RT "Mutations in multiple components of the nuclear pore complex cause RT nephrotic syndrome."; RL J. Clin. Invest. 128:4313-4328(2018). CC -!- FUNCTION: Component of the nuclear pore complex (NPC) that seems to be CC required for NPC assembly and maintenance (By similarity). Involved in CC nephrogenesis (PubMed:30179222). {ECO:0000250|UniProtKB:Q9BW27, CC ECO:0000269|PubMed:30179222}. CC -!- SUBUNIT: Component of the nuclear pore complex (NPC). CC {ECO:0000250|UniProtKB:Q9BW27}. CC -!- SUBCELLULAR LOCATION: Nucleus, nuclear pore complex CC {ECO:0000250|UniProtKB:Q9BW27}. Nucleus membrane CC {ECO:0000250|UniProtKB:Q9BW27}. CC -!- DISRUPTION PHENOTYPE: Morpholino knockdown of nup85 results in abnormal CC pronephric development characterized by underdeveloped to completely CC absent pronephros. {ECO:0000269|PubMed:30179222}. CC -!- SIMILARITY: Belongs to the nucleoporin Nup85 family. {ECO:0000305}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; BC074592; AAH74592.1; -; mRNA. DR RefSeq; NP_001004817.1; NM_001004817.1. DR AlphaFoldDB; Q6DK84; -. DR SMR; Q6DK84; -. DR STRING; 8364.ENSXETP00000046963; -. DR PaxDb; 8364-ENSXETP00000056747; -. DR DNASU; 448067; -. DR GeneID; 448067; -. DR KEGG; xtr:448067; -. DR AGR; Xenbase:XB-GENE-1008050; -. DR CTD; 79902; -. DR Xenbase; XB-GENE-1008050; nup85. DR eggNOG; KOG2271; Eukaryota. DR HOGENOM; CLU_027342_0_0_1; -. DR InParanoid; Q6DK84; -. DR OMA; ELMEWLN; -. DR OrthoDB; 3141715at2759; -. DR PhylomeDB; Q6DK84; -. DR TreeFam; TF323240; -. DR Reactome; R-XTR-141444; Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal. DR Reactome; R-XTR-170822; Regulation of Glucokinase by Glucokinase Regulatory Protein. DR Reactome; R-XTR-2467813; Separation of Sister Chromatids. DR Reactome; R-XTR-2500257; Resolution of Sister Chromatid Cohesion. DR Reactome; R-XTR-3108214; SUMOylation of DNA damage response and repair proteins. DR Reactome; R-XTR-3232142; SUMOylation of ubiquitinylation proteins. DR Reactome; R-XTR-3301854; Nuclear Pore Complex (NPC) Disassembly. DR Reactome; R-XTR-3371453; Regulation of HSF1-mediated heat shock response. DR Reactome; R-XTR-4085377; SUMOylation of SUMOylation proteins. DR Reactome; R-XTR-4570464; SUMOylation of RNA binding proteins. DR Reactome; R-XTR-4615885; SUMOylation of DNA replication proteins. DR Reactome; R-XTR-5663220; RHO GTPases Activate Formins. DR Reactome; R-XTR-68877; Mitotic Prometaphase. DR Reactome; R-XTR-9615933; Postmitotic nuclear pore complex (NPC) reformation. DR Reactome; R-XTR-9648025; EML4 and NUDC in mitotic spindle formation. DR Proteomes; UP000008143; Chromosome 10. DR Bgee; ENSXETG00000027060; Expressed in gastrula and 13 other cell types or tissues. DR ExpressionAtlas; Q6DK84; differential. DR GO; GO:0031965; C:nuclear membrane; IEA:UniProtKB-SubCell. DR GO; GO:0031080; C:nuclear pore outer ring; ISS:UniProtKB. DR GO; GO:0051028; P:mRNA transport; IEA:UniProtKB-KW. DR GO; GO:0072006; P:nephron development; IMP:UniProtKB. DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW. DR InterPro; IPR011502; Nucleoporin_Nup85. DR PANTHER; PTHR13373; FROUNT PROTEIN-RELATED; 1. DR PANTHER; PTHR13373:SF21; NUCLEAR PORE COMPLEX PROTEIN NUP85; 1. DR Pfam; PF07575; Nucleopor_Nup85; 1. PE 2: Evidence at transcript level; KW Membrane; mRNA transport; Nuclear pore complex; Nucleus; Protein transport; KW Reference proteome; Translocation; Transport. FT CHAIN 1..653 FT /note="Nuclear pore complex protein Nup85" FT /id="PRO_0000324191" SQ SEQUENCE 653 AA; 74918 MW; FCF9A8F5C0EAAB1F CRC64; MEELDVDPAE TPIPGLGQQN RHIGFSWGPG DLLLYETLYQ KQGSETAARC PFMYLVRSDE DIYSPVLRKL FNESHSIFVG LQKSAEENAG KSRKAQLVQV SRNYRSVLRA CMEEMHALSE STREPSQNTK YISQISILSA MELSWNLCEI LFIESAPAGP LLILLLDWVR LHVCEVDNIV QDVLRSERPT EHEKFWDGVT GYVLQGRMNE ARQLLAKEAS SSVSARSMCR VLDDLLKKMP MLNTAGTQTL TEFELKWQHW REECERHLQN GTFSSNPHME VVCRVLVGDE EVILEKRDLM TTWYHFLVSR LLFKHPTVKP TELHFYAQSS LDMFLAGNSS PEPLDNILLA AFEFDIHQVI KEFSIVSSNW WFVAHLTDLL DHCQLFQAHN LYFGANMREF LLLDYASGLF SHHSLWQLGV DYFDYCPNLG HVYLELHMER VPLNTEKKAL KALRICEKRQ MTEQVRSICK TMAMQSLCNG RLGSALSWSI RAKDAAFATL ISDRFLKEYS ERGNFTDLDL IDNLGSAMLL SDRLTFLGKY REFHRMYSQE QFSEAASLLL SLMTARIAPC SFWLTLLLDA LPLLEQKQVI FSAEQTYELM RCLEDRMAAK LDSTSPDEIQ KQDSVDSTKI EMLRLALARN LARAIVTEGA LQE //