ID Q6DEG0_DANRE Unreviewed; 573 AA. AC Q6DEG0; A0A8M1N1B0; DT 16-AUG-2004, integrated into UniProtKB/TrEMBL. DT 16-AUG-2004, sequence version 1. DT 13-SEP-2023, entry version 166. DE RecName: Full=TNF receptor-associated factor {ECO:0000256|PIRNR:PIRNR015614}; DE EC=2.3.2.27 {ECO:0000256|PIRNR:PIRNR015614}; GN Name=zgc:92367 {ECO:0000313|RefSeq:NP_001003513.1}; GN OrderedLocusNames=traf3 {ECO:0000313|EMBL:AAH77157.1, GN ECO:0000313|ZFIN:ZDB-GENE-040801-257}; OS Danio rerio (Zebrafish) (Brachydanio rerio). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes; OC Danionidae; Danioninae; Danio. OX NCBI_TaxID=7955 {ECO:0000313|EMBL:AAH77157.1}; RN [1] {ECO:0000313|RefSeq:NP_001003513.1} RP NUCLEOTIDE SEQUENCE. RX PubMed=15520368; DOI=10.1073/pnas.0407241101; RA Song H.D., Sun X.J., Deng M., Zhang G.W., Zhou Y., Wu X.Y., Sheng Y., RA Chen Y., Ruan Z., Jiang C.L., Fan H.Y., Zon L.I., Kanki J.P., Liu T.X., RA Look A.T., Chen Z.; RT "Hematopoietic gene expression profile in zebrafish kidney marrow."; RL Proc. Natl. Acad. Sci. U.S.A. 101:16240-16245(2004). RN [2] {ECO:0000313|EMBL:AAH77157.1} RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RC TISSUE=Whole {ECO:0000313|EMBL:AAH77157.1}; RG NIH - Zebrafish Gene Collection (ZGC) project; RL Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases. RN [3] {ECO:0000313|RefSeq:NP_001003513.1} RP NUCLEOTIDE SEQUENCE. RX PubMed=18039395; RA Stein C., Caccamo M., Laird G., Leptin M.; RT "Conservation and divergence of gene families encoding components of innate RT immune response systems in zebrafish."; RL Genome Biol. 8:R251-R251(2007). RN [4] {ECO:0000313|RefSeq:NP_001003513.1} RP NUCLEOTIDE SEQUENCE. RX PubMed=22615868; RA He B.L., Yuan J.M., Yang L.Y., Xie J.F., Weng S.P., Yu X.Q., He J.G.; RT "The viral TRAF protein (ORF111L) from infectious spleen and kidney RT necrosis virus interacts with TRADD and induces caspase 8-mediated RT apoptosis."; RL PLoS ONE 7:e37001-e37001(2012). RN [5] {ECO:0000313|Ensembl:ENSDARP00000038596} RP IDENTIFICATION. RC STRAIN=Tuebingen {ECO:0000313|Ensembl:ENSDARP00000038596}; RG Ensembl; RL Submitted (FEB-2012) to UniProtKB. RN [6] {ECO:0000313|RefSeq:NP_001003513.1} RP NUCLEOTIDE SEQUENCE. RX PubMed=23947337; RA Wang Y.C., Lin C., Chuang M.T., Hsieh W.P., Lan C.Y., Chuang Y.J., RA Chen B.S.; RT "Interspecies protein-protein interaction network construction for RT characterization of host-pathogen interactions: a Candida albicans- RT zebrafish interaction study."; RL BMC Syst. Biol. 7:79-79(2013). RN [7] {ECO:0000313|Ensembl:ENSDARP00000038596, ECO:0000313|Proteomes:UP000000437} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=Tuebingen {ECO:0000313|Ensembl:ENSDARP00000038596}; RX PubMed=23594743; DOI=10.1038/nature12111; RG Genome Reference Consortium Zebrafish; RA Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M., RA Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I., RA Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J., RA White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y., RA Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B., RA Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S., RA Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M., RA Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J., RA Clee C., Oliver K., Clark R., Riddle C., Elliot D., Eliott D., RA Threadgold G., Harden G., Ware D., Begum S., Mortimore B., Mortimer B., RA Kerry G., Heath P., Phillimore B., Tracey A., Corby N., Dunn M., RA Johnson C., Wood J., Clark S., Pelan S., Griffiths G., Smith M., RA Glithero R., Howden P., Barker N., Lloyd C., Stevens C., Harley J., RA Holt K., Panagiotidis G., Lovell J., Beasley H., Henderson C., Gordon D., RA Auger K., Wright D., Collins J., Raisen C., Dyer L., Leung K., RA Robertson L., Ambridge K., Leongamornlert D., McGuire S., Gilderthorp R., RA Griffiths C., Manthravadi D., Nichol S., Barker G., Whitehead S., Kay M., RA Brown J., Murnane C., Gray E., Humphries M., Sycamore N., Barker D., RA Saunders D., Wallis J., Babbage A., Hammond S., Mashreghi-Mohammadi M., RA Barr L., Martin S., Wray P., Ellington A., Matthews N., Ellwood M., RA Woodmansey R., Clark G., Cooper J., Cooper J., Tromans A., Grafham D., RA Skuce C., Pandian R., Andrews R., Harrison E., Kimberley A., Garnett J., RA Fosker N., Hall R., Garner P., Kelly D., Bird C., Palmer S., Gehring I., RA Berger A., Dooley C.M., Ersan-Urun Z., Eser C., Geiger H., Geisler M., RA Karotki L., Kirn A., Konantz J., Konantz M., Oberlander M., RA Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G., Osoegawa K., Zhu B., RA Rapp A., Widaa S., Langford C., Yang F., Schuster S.C., Carter N.P., RA Harrow J., Ning Z., Herrero J., Searle S.M., Enright A., Geisler R., RA Plasterk R.H., Lee C., Westerfield M., de Jong P.J., Zon L.I., RA Postlethwait J.H., Nusslein-Volhard C., Hubbard T.J., Roest Crollius H., RA Rogers J., Stemple D.L.; RT "The zebrafish reference genome sequence and its relationship to the human RT genome."; RL Nature 496:498-503(2013). RN [8] {ECO:0000313|RefSeq:NP_001003513.1} RP NUCLEOTIDE SEQUENCE. RX PubMed=26469318; RA Elkon R., Milon B., Morrison L., Shah M., Vijayakumar S., Racherla M., RA Leitch C.C., Silipino L., Hadi S., Weiss-Gayet M., Barras E., Schmid C.D., RA Ait-Lounis A., Barnes A., Song Y., Eisenman D.J., Eliyahu E., RA Frolenkov G.I., Strome S.E., Durand B., Zaghloul N.A., Jones S.M., RA Reith W., Hertzano R.; RT "RFX transcription factors are essential for hearing in mice."; RL Nat. Commun. 6:8549-8549(2015). RN [9] {ECO:0000313|RefSeq:NP_001003513.1} RP NUCLEOTIDE SEQUENCE. RX PubMed=29142761; RA Srivastava N., Shelly A., Kumar M., Pant A., Das B., Majumdar T., RA Mazumder S.; RT "Aeromonas hydrophila utilizes TLR4 topology for synchronous activation of RT MyD88 and TRIF to orchestrate anti-inflammatory responses in zebrafish."; RL Cell. Death. Discov. 3:17067-17067(2017). RN [10] {ECO:0000313|RefSeq:NP_001003513.1} RP NUCLEOTIDE SEQUENCE. RX PubMed=29407611; RA Zhang W., Jia P., Liu W., Li Y., Yi M., Jia K.; RT "Functional characterization of tumor necrosis factor receptor-associated RT factor 3 of sea perch (Lateolabrax japonicas) in innate immune."; RL Fish Shellfish Immunol. 75:1-7(2018). RN [11] {ECO:0000313|RefSeq:NP_001003513.1} RP NUCLEOTIDE SEQUENCE. RX PubMed=30508673; RA Lai Y., Liang M., Hu, Zeng Z., Lin H., Yi G., Li M., Liu Z.; RT "RNF135 is a positive regulator of IFN expression and involved in RIG-I RT signaling pathway by targeting RIG-I."; RL Fish Shellfish Immunol. 86:474-479(2019). RN [12] {ECO:0000313|RefSeq:NP_001003513.1} RP IDENTIFICATION. RG RefSeq; RL Submitted (MAR-2023) to UniProtKB. CC -!- CATALYTIC ACTIVITY: CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L- CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.; CC EC=2.3.2.27; Evidence={ECO:0000256|PIRNR:PIRNR015614}; CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496, CC ECO:0000256|PIRNR:PIRNR015614}. CC -!- SIMILARITY: Belongs to the TNF receptor-associated factor family. CC {ECO:0000256|PIRNR:PIRNR015614}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; BX511189; -; NOT_ANNOTATED_CDS; Genomic_DNA. DR EMBL; CR392327; -; NOT_ANNOTATED_CDS; Genomic_DNA. DR EMBL; BC077157; AAH77157.1; -; mRNA. DR RefSeq; NP_001003513.1; NM_001003513.1. DR STRING; 7955.ENSDARP00000105765; -. DR Ensembl; ENSDART00000031458.8; ENSDARP00000038596.7; ENSDARG00000022000.10. DR GeneID; 445119; -. DR KEGG; dre:445119; -. DR CTD; 7187; -. DR ZFIN; ZDB-GENE-040801-257; traf3. DR eggNOG; KOG0297; Eukaryota. DR HOGENOM; CLU_021061_4_1_1; -. DR OMA; ETVCPSF; -. DR OrthoDB; 2913784at2759; -. DR TreeFam; TF321154; -. DR Reactome; R-DRE-5668541; TNFR2 non-canonical NF-kB pathway. DR Reactome; R-DRE-5676594; TNF receptor superfamily (TNFSF) members mediating non-canonical NF-kB pathway. DR Reactome; R-DRE-5689896; Ovarian tumor domain proteases. DR Reactome; R-DRE-936964; Activation of IRF3, IRF7 mediated by TBK1, IKBKE. DR Proteomes; UP000000437; Chromosome 17. DR Bgee; ENSDARG00000022000; Expressed in spleen and 20 other tissues. DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell. DR GO; GO:0005164; F:tumor necrosis factor receptor binding; IEA:UniProtKB-UniRule. DR GO; GO:0031625; F:ubiquitin protein ligase binding; IBA:GO_Central. DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule. DR GO; GO:0006915; P:apoptotic process; IEA:UniProtKB-KW. DR GO; GO:0045087; P:innate immune response; IEA:InterPro. DR GO; GO:0032088; P:negative regulation of NF-kappaB transcription factor activity; IBA:GO_Central. DR GO; GO:0046330; P:positive regulation of JNK cascade; IBA:GO_Central. DR GO; GO:0070534; P:protein K63-linked ubiquitination; IBA:GO_Central. DR GO; GO:0042981; P:regulation of apoptotic process; IEA:InterPro. DR GO; GO:0001817; P:regulation of cytokine production; IEA:InterPro. DR GO; GO:0050688; P:regulation of defense response to virus; IEA:InterPro. DR GO; GO:0043122; P:regulation of I-kappaB kinase/NF-kappaB signaling; IBA:GO_Central. DR GO; GO:0001878; P:response to yeast; IDA:ZFIN. DR GO; GO:0008063; P:Toll signaling pathway; IEA:InterPro. DR GO; GO:0033209; P:tumor necrosis factor-mediated signaling pathway; IBA:GO_Central. DR CDD; cd03777; MATH_TRAF3; 1. DR CDD; cd16640; RING-HC_TRAF3; 1. DR Gene3D; 3.30.40.10; Zinc/RING finger domain, C3HC4 (zinc finger); 3. DR InterPro; IPR002083; MATH/TRAF_dom. DR InterPro; IPR012227; TNF_rcpt-assoc_TRAF_met. DR InterPro; IPR008974; TRAF-like. DR InterPro; IPR037304; TRAF3_MATH. DR InterPro; IPR027128; TRAF3_RING-HC. DR InterPro; IPR018957; Znf_C3HC4_RING-type. DR InterPro; IPR001841; Znf_RING. DR InterPro; IPR013083; Znf_RING/FYVE/PHD. DR InterPro; IPR017907; Znf_RING_CS. DR InterPro; IPR001293; Znf_TRAF. DR PANTHER; PTHR10131; TNF RECEPTOR ASSOCIATED FACTOR; 1. DR PANTHER; PTHR10131:SF76; TNF RECEPTOR-ASSOCIATED FACTOR 3; 1. DR Pfam; PF00097; zf-C3HC4; 1. DR Pfam; PF02176; zf-TRAF; 1. DR PIRSF; PIRSF015614; TRAF; 1. DR SMART; SM00061; MATH; 1. DR SMART; SM00184; RING; 1. DR SUPFAM; SSF57850; RING/U-box; 1. DR SUPFAM; SSF49599; TRAF domain-like; 3. DR SUPFAM; SSF57953; Trimerization domain of TRAF; 1. DR PROSITE; PS50144; MATH; 1. DR PROSITE; PS00518; ZF_RING_1; 1. DR PROSITE; PS50089; ZF_RING_2; 1. DR PROSITE; PS50145; ZF_TRAF; 2. PE 2: Evidence at transcript level; KW Apoptosis {ECO:0000256|ARBA:ARBA00022703}; KW Coiled coil {ECO:0000256|ARBA:ARBA00023054, ECO:0000256|SAM:Coils}; KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490, ECO:0000256|PIRNR:PIRNR015614}; KW Isopeptide bond {ECO:0000256|ARBA:ARBA00022499}; KW Metal-binding {ECO:0000256|ARBA:ARBA00022723, KW ECO:0000256|PIRNR:PIRNR015614}; KW Receptor {ECO:0000313|EMBL:AAH77157.1, ECO:0000313|RefSeq:NP_001003513.1}; KW Reference proteome {ECO:0000313|Proteomes:UP000000437}; KW Zinc {ECO:0000256|ARBA:ARBA00022833, ECO:0000256|PIRNR:PIRNR015614}; KW Zinc-finger {ECO:0000256|ARBA:ARBA00022771, ECO:0000256|PROSITE- KW ProRule:PRU00207}. FT DOMAIN 56..94 FT /note="RING-type" FT /evidence="ECO:0000259|PROSITE:PS50089" FT DOMAIN 139..192 FT /note="TRAF-type" FT /evidence="ECO:0000259|PROSITE:PS50145" FT DOMAIN 194..251 FT /note="TRAF-type" FT /evidence="ECO:0000259|PROSITE:PS50145" FT DOMAIN 420..565 FT /note="MATH" FT /evidence="ECO:0000259|PROSITE:PS50144" FT ZN_FING 139..192 FT /note="TRAF-type" FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00207" FT ZN_FING 194..251 FT /note="TRAF-type" FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00207" FT REGION 1..34 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT COILED 290..324 FT /evidence="ECO:0000256|SAM:Coils" FT COMPBIAS 1..21 FT /note="Polar residues" FT /evidence="ECO:0000256|SAM:MobiDB-lite" SQ SEQUENCE 573 AA; 65608 MW; F7C441E6725833C2 CRC64; MSAGRNVEQQ IPLQQRPPSL AMPSMAQRPR PEPGFSPLHG GFRDHFVTTP EPKYCCETCR LVLCNPRQTE CGHRFCESCI NELLSKPNPV CPADLLPLFE DKIFRDVCCN REIMALKVYC RSEKNGCKEQ MCLQQVMEHL VICPYFEVPC PLGKCKEKMM RKDMPEHLSR KCKHREVTCE FCSLKMALTE LQKHKETVCP AFPVACPNHC SFSSILRSEL SSHQHDCPKA QVTCSFIRYG CSYKGLNQEM REHESSFASE HLRMMAVRNT TLEAKVEDVK SELMERYKVL PSLSSRLAEV ERQYEEMREK NRQLEQKLVS MQMLMSSHSE KLLEVEMELR ELRPLRAMRE EVETLRGSVE SMRSMVSSLD SSCVNSASGS HTLASLEQQL TRHDDLMSVH DVRLAEMDLK LQVLETASFN GTLIWKIRDY KRRKQEAVVS KTLSLYSQPF YTGYFGYKMC ARVYLNGDGM GKGTHLSLFF VVMRGEYDAL LQWPFKQKVT LMLMDQGPAR KHLGDAFKPD PSSSSFRRPT GEMNIASGCP LFVAQTVLEN GTYIKDDTIF IKVTVDTSDL PDP //