ID PUS4_ORYSJ Reviewed; 448 AA. AC Q69K07; A0A0P0XJK3; DT 14-APR-2009, integrated into UniProtKB/Swiss-Prot. DT 13-SEP-2004, sequence version 1. DT 23-FEB-2022, entry version 87. DE RecName: Full=RNA pseudouridine synthase 4, mitochondrial; DE EC=5.4.99.-; DE AltName: Full=RNA pseudouridylate synthase 4; DE AltName: Full=RNA-uridine isomerase 4; DE Flags: Precursor; GN OrderedLocusNames=Os09g0103500, LOC_Os09g01620; GN ORFNames=OSJNBa0006G10.3, P0414D03.35; OS Oryza sativa subsp. japonica (Rice). OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade; OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa. OX NCBI_TaxID=39947; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=cv. Nipponbare; RX PubMed=16100779; DOI=10.1038/nature03895; RG International rice genome sequencing project (IRGSP); RT "The map-based sequence of the rice genome."; RL Nature 436:793-800(2005). RN [2] RP GENOME REANNOTATION. RC STRAIN=cv. Nipponbare; RX PubMed=18089549; DOI=10.1093/nar/gkm978; RG The rice annotation project (RAP); RT "The rice annotation project database (RAP-DB): 2008 update."; RL Nucleic Acids Res. 36:D1028-D1033(2008). RN [3] RP GENOME REANNOTATION. RC STRAIN=cv. Nipponbare; RX PubMed=24280374; DOI=10.1186/1939-8433-6-4; RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R., RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L., RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H., RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.; RT "Improvement of the Oryza sativa Nipponbare reference genome using next RT generation sequence and optical map data."; RL Rice 6:4-4(2013). RN [4] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RC STRAIN=cv. Nipponbare; RX PubMed=12869764; DOI=10.1126/science.1081288; RG The rice full-length cDNA consortium; RT "Collection, mapping, and annotation of over 28,000 cDNA clones from RT japonica rice."; RL Science 301:376-379(2003). CC -!- CATALYTIC ACTIVITY: CC Reaction=a uridine in RNA = a pseudouridine in RNA; CC Xref=Rhea:RHEA:48348, Rhea:RHEA-COMP:12068, Rhea:RHEA-COMP:12069, CC ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; CC -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000305}. CC -!- SIMILARITY: Belongs to the pseudouridine synthase RluA family. CC {ECO:0000305}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AP005909; BAD34146.1; -; Genomic_DNA. DR EMBL; AP006061; BAD36694.1; -; Genomic_DNA. DR EMBL; AP008215; BAF24470.1; -; Genomic_DNA. DR EMBL; AP014965; BAT06782.1; -; Genomic_DNA. DR EMBL; AK065573; -; NOT_ANNOTATED_CDS; mRNA. DR RefSeq; XP_015610651.1; XM_015755165.1. DR STRING; 4530.OS09T0103500-01; -. DR PaxDb; Q69K07; -. DR EnsemblPlants; Os09t0103500-01; Os09t0103500-01; Os09g0103500. DR GeneID; 4346373; -. DR Gramene; Os09t0103500-01; Os09t0103500-01; Os09g0103500. DR KEGG; osa:4346373; -. DR eggNOG; KOG1919; Eukaryota. DR HOGENOM; CLU_016902_13_1_1; -. DR InParanoid; Q69K07; -. DR OMA; KWVRRCC; -. DR Proteomes; UP000000763; Chromosome 9. DR Proteomes; UP000059680; Chromosome 9. DR Genevisible; Q69K07; OS. DR GO; GO:0005739; C:mitochondrion; IEA:UniProtKB-SubCell. DR GO; GO:0009982; F:pseudouridine synthase activity; IBA:GO_Central. DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW. DR GO; GO:0000455; P:enzyme-directed rRNA pseudouridine synthesis; IBA:GO_Central. DR CDD; cd02869; PseudoU_synth_RluA_like; 1. DR InterPro; IPR020103; PsdUridine_synth_cat_dom_sf. DR InterPro; IPR006145; PsdUridine_synth_RsuA/RluA. DR Pfam; PF00849; PseudoU_synth_2; 1. DR SUPFAM; SSF55120; SSF55120; 1. PE 2: Evidence at transcript level; KW Isomerase; Mitochondrion; Reference proteome; RNA-binding; Transit peptide. FT TRANSIT 1..69 FT /note="Mitochondrion" FT /evidence="ECO:0000255" FT CHAIN 70..448 FT /note="RNA pseudouridine synthase 4, mitochondrial" FT /id="PRO_0000368025" FT DOMAIN 84..170 FT /note="S4 RNA-binding" FT ACT_SITE 228 FT /evidence="ECO:0000250" FT CONFLICT 32 FT /note="R -> S (in Ref. 4; AK065573)" FT /evidence="ECO:0000305" FT CONFLICT 79 FT /note="D -> G (in Ref. 4; AK065573)" FT /evidence="ECO:0000305" SQ SEQUENCE 448 AA; 49035 MW; 85AD88515EB7283F CRC64; MAALLYLRRR AAAAALAGVA PKPQWLATAA RRGALVSGDD GGETGERGKS PWLQLPPFAP LDAAAAARAI SRGGGEGGDG EQGATAIKWV RRCCPDLPTS LVQKLFRLRK VKKNVVTAEI SSADASAEQH RLRRVSAKDQ LMPGDILFLP VNLKESSVAE KTKKFDNRNE INFLRGLEIY KDEAIIVVNK PPGMPVQGGV GIKNSIDVLA SMFEENSSEA PRLVHRLDRD CSGILVLGRN QLSTSMLHAI FREKTADALA DGTQHVLQRK YVALVIGTPR HPKGLLSAPL AKILLQDGKS ERLTIRASSN AASVQDALTE YRVIESCPQG YTWLELFPRT GRKHQRFLEP QLLGITSMDG KRIRSGCLFP CHGQLMRNCS GKGSFPLGLL WVAEASLRSN LSFIYTASKW FFLMSQWLFI GCSLQTLILI SQILRSSTLL LHCRCICG //