ID PUS4_ORYSJ Reviewed; 448 AA. AC Q69K07; DT 14-APR-2009, integrated into UniProtKB/Swiss-Prot. DT 13-SEP-2004, sequence version 1. DT 28-JUL-2009, entry version 25. DE RecName: Full=RNA pseudourine synthase 4, mitochondrial; DE EC=5.4.99.-; DE AltName: Full=RNA-uridine isomerase 4; DE AltName: Full=RNA pseudouridylate synthase 4; DE Flags: Precursor; GN OrderedLocusNames=Os09g0103500, LOC_Os09g01620; GN ORFNames=OSJNBa0006G10.3, P0414D03.35; OS Oryza sativa subsp. japonica (Rice). OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP clade; OC Ehrhartoideae; Oryzeae; Oryza. OX NCBI_TaxID=39947; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=cv. Nipponbare; RX PubMed=16100779; DOI=10.1038/nature03895; RG International rice genome sequencing project (IRGSP); RT "The map-based sequence of the rice genome."; RL Nature 436:793-800(2005). RN [2] RP GENOME REANNOTATION. RC STRAIN=cv. Nipponbare; RX PubMed=17210932; DOI=10.1101/gr.5509507; RG The rice annotation project (RAP); RT "Curated genome annotation of Oryza sativa ssp. japonica and RT comparative genome analysis with Arabidopsis thaliana."; RL Genome Res. 17:175-183(2007). RN [3] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RC STRAIN=cv. Nipponbare; RX MEDLINE=22752273; PubMed=12869764; DOI=10.1126/science.1081288; RG The rice full-length cDNA consortium; RT "Collection, mapping, and annotation of over 28,000 cDNA clones from RT japonica rice."; RL Science 301:376-379(2003). CC -!- CATALYTIC ACTIVITY: RNA uridine = RNA pseudouridine. CC -!- SUBCELLULAR LOCATION: Mitochondrion (Potential). CC -!- SIMILARITY: Belongs to the pseudouridine synthase rluA family. CC -!- SIMILARITY: Contains 1 S4 RNA-binding domain. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AP005909; BAD34146.1; -; Genomic_DNA. DR EMBL; AP006061; BAD36694.1; -; Genomic_DNA. DR EMBL; AP008215; BAF24470.1; -; Genomic_DNA. DR EMBL; AK065573; -; NOT_ANNOTATED_CDS; mRNA. DR RefSeq; NP_001062556.1; -. DR UniGene; Os.52200; -. DR GeneID; 4346373; -. DR KEGG; osa:4346373; -. DR Gramene; Q69K07; -. DR GO; GO:0005739; C:mitochondrion; IEA:UniProtKB-SubCell. DR GO; GO:0009982; F:pseudouridine synthase activity; IEA:InterPro. DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW. DR GO; GO:0001522; P:pseudouridine synthesis; IEA:InterPro. DR InterPro; IPR006224; PsdUridine_synth_RluC/D_CS. DR InterPro; IPR006145; PsdUridine_synth_RsuA/RluD. DR Pfam; PF00849; PseudoU_synth_2; 1. DR ProDom; PD001819; PseudoU_synth; 1. DR PROSITE; PS01129; PSI_RLU; FALSE_NEG. DR PROSITE; PS50889; S4; FALSE_NEG. PE 2: Evidence at transcript level; KW Isomerase; Mitochondrion; RNA-binding; Transit peptide. FT TRANSIT 1 69 Mitochondrion (Potential). FT CHAIN 70 448 RNA pseudourine synthase 4, FT mitochondrial. FT /FTId=PRO_0000368025. FT DOMAIN 84 170 S4 RNA-binding. FT ACT_SITE 228 228 By similarity. FT CONFLICT 32 32 R -> S (in Ref. 3; AK065573). FT CONFLICT 79 79 D -> G (in Ref. 3; AK065573). SQ SEQUENCE 448 AA; 49035 MW; 85AD88515EB7283F CRC64; MAALLYLRRR AAAAALAGVA PKPQWLATAA RRGALVSGDD GGETGERGKS PWLQLPPFAP LDAAAAARAI SRGGGEGGDG EQGATAIKWV RRCCPDLPTS LVQKLFRLRK VKKNVVTAEI SSADASAEQH RLRRVSAKDQ LMPGDILFLP VNLKESSVAE KTKKFDNRNE INFLRGLEIY KDEAIIVVNK PPGMPVQGGV GIKNSIDVLA SMFEENSSEA PRLVHRLDRD CSGILVLGRN QLSTSMLHAI FREKTADALA DGTQHVLQRK YVALVIGTPR HPKGLLSAPL AKILLQDGKS ERLTIRASSN AASVQDALTE YRVIESCPQG YTWLELFPRT GRKHQRFLEP QLLGITSMDG KRIRSGCLFP CHGQLMRNCS GKGSFPLGLL WVAEASLRSN LSFIYTASKW FFLMSQWLFI GCSLQTLILI SQILRSSTLL LHCRCICG //