ID APC5A_XENLA Reviewed; 150 AA. AC Q68FI4; DT 10-OCT-2018, integrated into UniProtKB/Swiss-Prot. DT 11-OCT-2004, sequence version 1. DT 08-MAY-2019, entry version 77. DE RecName: Full=Actin-related protein 2/3 complex subunit 5-A {ECO:0000305}; GN Name=arpc5-a; GN ORFNames=XELAEV_18023257mg {ECO:0000312|EMBL:OCT85093.1}; OS Xenopus laevis (African clawed frog). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Amphibia; Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; OC Xenopus. OX NCBI_TaxID=8355; RN [1] RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND SUBCELLULAR LOCATION. RX PubMed=17178911; DOI=10.1083/jcb.200604176; RA Miyoshi T., Tsuji T., Higashida C., Hertzog M., Fujita A., RA Narumiya S., Scita G., Watanabe N.; RT "Actin turnover-dependent fast dissociation of capping protein in the RT dendritic nucleation actin network: evidence of frequent filament RT severing."; RL J. Cell Biol. 175:947-955(2006). RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=J; RX PubMed=27762356; DOI=10.1038/nature19840; RA Session A.M., Uno Y., Kwon T., Chapman J.A., Toyoda A., Takahashi S., RA Fukui A., Hikosaka A., Suzuki A., Kondo M., van Heeringen S.J., RA Quigley I., Heinz S., Ogino H., Ochi H., Hellsten U., Lyons J.B., RA Simakov O., Putnam N., Stites J., Kuroki Y., Tanaka T., Michiue T., RA Watanabe M., Bogdanovic O., Lister R., Georgiou G., Paranjpe S.S., RA van Kruijsbergen I., Shu S., Carlson J., Kinoshita T., Ohta Y., RA Mawaribuchi S., Jenkins J., Grimwood J., Schmutz J., Mitros T., RA Mozaffari S.V., Suzuki Y., Haramoto Y., Yamamoto T.S., Takagi C., RA Heald R., Miller K., Haudenschild C., Kitzman J., Nakayama T., RA Izutsu Y., Robert J., Fortriede J., Burns K., Lotay V., Karimi K., RA Yasuoka Y., Dichmann D.S., Flajnik M.F., Houston D.W., Shendure J., RA DuPasquier L., Vize P.D., Zorn A.M., Ito M., Marcotte E.M., RA Wallingford J.B., Ito Y., Asashima M., Ueno N., Matsuda Y., RA Veenstra G.J., Fujiyama A., Harland R.M., Taira M., Rokhsar D.S.; RT "Genome evolution in the allotetraploid frog Xenopus laevis."; RL Nature 538:336-343(2016). RN [3] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RC TISSUE=Kidney; RG NIH - Xenopus Gene Collection (XGC) project; RL Submitted (AUG-2004) to the EMBL/GenBank/DDBJ databases. CC -!- FUNCTION: Component of the Arp2/3 complex, a multiprotein complex CC that mediates actin polymerization upon stimulation by nucleation- CC promoting factor (NPF) (PubMed:17178911). The Arp2/3 complex CC mediates the formation of branched actin networks in the CC cytoplasm, providing the force for cell motility CC (PubMed:17178911). In addition to its role in the cytoplasmic CC cytoskeleton, the Arp2/3 complex also promotes actin CC polymerization in the nucleus, thereby regulating gene CC transcription and repair of damaged DNA (By similarity). The CC Arp2/3 complex promotes homologous recombination (HR) repair in CC response to DNA damage by promoting nuclear actin polymerization, CC leading to drive motility of double-strand breaks (DSBs) (By CC similarity). {ECO:0000250|UniProtKB:O15511, CC ECO:0000250|UniProtKB:Q6DE18, ECO:0000269|PubMed:17178911}. CC -!- SUBUNIT: Component of the Arp2/3 complex composed of actr2/arp2, CC actr3/arp3, arpc1 (arpc1a or arpc1b), arpc2, arpc3, arpc4 and CC arpc5. {ECO:0000250|UniProtKB:Q6DE18}. CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton CC {ECO:0000269|PubMed:17178911}. Cell projection CC {ECO:0000269|PubMed:17178911}. Nucleus CC {ECO:0000250|UniProtKB:Q6DE18}. CC -!- SIMILARITY: Belongs to the ARPC5 family. {ECO:0000305}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; EF011868; ABL63901.1; -; mRNA. DR EMBL; CM004472; OCT85093.1; -; Genomic_DNA. DR EMBL; BC079805; AAH79805.1; -; mRNA. DR RefSeq; NP_001087450.1; NM_001093981.1. DR IntAct; Q68FI4; 5. DR MINT; Q68FI4; -. DR GeneID; 447274; -. DR KEGG; xla:447274; -. DR CTD; 447274; -. DR Xenbase; XB-GENE-866317; arpc5. DR KO; K05754; -. DR OrthoDB; 1565115at2759; -. DR Proteomes; UP000186698; Chromosome 4l. DR Proteomes; UP000186698; Unassembled WGS sequence. DR GO; GO:0005885; C:Arp2/3 protein complex; ISS:UniProtKB. DR GO; GO:0042995; C:cell projection; IEA:UniProtKB-SubCell. DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW. DR GO; GO:0005634; C:nucleus; ISS:UniProtKB. DR GO; GO:0035861; C:site of double-strand break; ISS:UniProtKB. DR GO; GO:0003779; F:actin binding; IEA:UniProtKB-KW. DR GO; GO:0034314; P:Arp2/3 complex-mediated actin nucleation; IEA:InterPro. DR Gene3D; 1.25.40.190; -; 1. DR InterPro; IPR006789; ARPC5. DR InterPro; IPR036743; ARPC5_sf. DR PANTHER; PTHR12644; PTHR12644; 1. DR Pfam; PF04699; P16-Arc; 1. DR PIRSF; PIRSF039096; p16-ARC; 1. DR SUPFAM; SSF69103; SSF69103; 1. PE 2: Evidence at transcript level; KW Actin-binding; Cell projection; Complete proteome; Cytoplasm; KW Cytoskeleton; Nucleus; Reference proteome. FT CHAIN 1 150 Actin-related protein 2/3 complex subunit FT 5-A. FT /FTId=PRO_0000445564. SQ SEQUENCE 150 AA; 16400 MW; 686E7C42BBDF5726 CRC64; MAKNTVSARF RKVDVDEYDE NKFVDEEEAG EGQQGPDEGE VDSAIRGGNM MGALQAALKN PPINTKNQSA KDRAENLVLK VLISFKANEI EKAVQSLDKN STDLLMKYIY KGFESPSDNS SAVLLQWHEK ALAVAGVGSI VRVLTARKTV //