ID KRP1_ARATH Reviewed; 191 AA. AC Q67Y93; O04154; O82809; Q8LDX1; DT 10-JUL-2007, integrated into UniProtKB/Swiss-Prot. DT 10-JUL-2007, sequence version 2. DT 03-OCT-2012, entry version 47. DE RecName: Full=Cyclin-dependent kinase inhibitor 1; DE AltName: Full=Inhibitor/interactor of CDK protein 1; DE AltName: Full=KIP-related protein 1; GN Name=KRP1; Synonyms=ICK1; OrderedLocusNames=At2g23430; GN ORFNames=F26B6.8; OS Arabidopsis thaliana (Mouse-ear cress). OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons; OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis. OX NCBI_TaxID=3702; RN [1] RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND INTERACTION WITH CDKA-1. RC STRAIN=cv. Columbia; RX MEDLINE=97242401; PubMed=9087400; DOI=10.1038/386451a0; RA Wang H., Fowke L.C., Crosby W.L.; RT "A plant cyclin-dependent kinase inhibitor gene."; RL Nature 386:451-452(1997). RN [2] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, TISSUE SPECIFICITY, RP INDUCTION, AND INTERACTION WITH CDKA-1 AND CYCD3-1. RC STRAIN=cv. Columbia; RX MEDLINE=98426383; PubMed=9753775; RX DOI=10.1046/j.1365-313X.1998.00231.x; RA Wang H., Qi Q., Schorr P., Cutler A.J., Crosby W.L., Fowke L.C.; RT "ICK1, a cyclin-dependent protein kinase inhibitor from Arabidopsis RT thaliana interacts with both Cdc2a and CycD3, and its expression is RT induced by abscisic acid."; RL Plant J. 15:501-510(1998). RN [3] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=cv. Columbia; RX MEDLINE=20083487; PubMed=10617197; DOI=10.1038/45471; RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., RA Moffat K.S., Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., RA Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., RA Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., RA Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., RA Venter J.C.; RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis RT thaliana."; RL Nature 402:761-768(1999). RN [4] RP GENOME REANNOTATION. RC STRAIN=cv. Columbia; RG The Arabidopsis Information Resource (TAIR); RL Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases. RN [5] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RC STRAIN=cv. Columbia; RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K., RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., RA Hayashizaki Y., Shinozaki K.; RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."; RL Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases. RN [6] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RC STRAIN=cv. Columbia; RA Bautista V.R., Kim C.J., Chen H., Quinitio C., Ecker J.R.; RT "Arabidopsis ORF clones."; RL Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases. RN [7] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., RA Feldmann K.A.; RT "Full-length cDNA from Arabidopsis thaliana."; RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases. RN [8] RP FUNCTION. RX PubMed=11123800; DOI=10.1046/j.1365-313x.2000.00899.x; RA Wang H., Zhou Y., Gilmer S., Whitwill S., Fowke L.C.; RT "Expression of the plant cyclin-dependent kinase inhibitor ICK1 RT affects cell division, plant growth and morphology."; RL Plant J. 24:613-623(2000). RN [9] RP INTERACTION WITH CDKA-1. RX MEDLINE=21342510; PubMed=11449057; DOI=10.1105/tpc.13.7.1653; RA de Veylder L., Beeckman T., Beemster G.T.S., Krols L., Terras F., RA Landrieu I., van der Schueren E., Maes S., Naudts M., Inze D.; RT "Functional analysis of cyclin-dependent kinase inhibitors of RT Arabidopsis."; RL Plant Cell 13:1653-1667(2001). RN [10] RP FUNCTION. RX PubMed=12029474; DOI=10.1007/s00425-002-0752-2; RA Zhou Y., Wang H., Gilmer S., Whitwill S., Keller W., Fowke L.C.; RT "Control of petal and pollen development by the plant cyclin-dependent RT kinase inhibitor ICK1 in transgenic Brassica plants."; RL Planta 215:248-257(2002). RN [11] RP DEVELOPMENTAL STAGE. RX PubMed=12000456; DOI=10.1046/j.1365-313X.2002.01274.x; RA Menges M., Murray J.A.H.; RT "Synchronous Arabidopsis suspension cultures for analysis of cell- RT cycle gene activity."; RL Plant J. 30:203-212(2002). RN [12] RP GENE FAMILY, AND NOMENCLATURE. RX PubMed=11971144; DOI=10.1105/tpc.010445; RA Vandepoele K., Raes J., de Veylder L., Rouze P., Rombauts S., Inze D.; RT "Genome-wide analysis of core cell cycle genes in Arabidopsis."; RL Plant Cell 14:903-916(2002). RN [13] RP DEVELOPMENTAL STAGE. RX PubMed=12368490; DOI=10.1105/tpc.004960; RA Himanen K., Boucheron E., Vanneste S., de Almeida Engler J., Inze D., RA Beeckman T.; RT "Auxin-mediated cell cycle activation during early lateral root RT initiation."; RL Plant Cell 14:2339-2351(2002). RN [14] RP INTERACTION WITH CYCD2-1. RX PubMed=12569402; DOI=10.1007/s00425-002-0935-x; RA Zhou Y., Wang H., Gilmer S., Whitwill S., Fowke L.C.; RT "Effects of co-expressing the plant CDK inhibitor ICK1 and D-type RT cyclin genes on plant growth, cell size and ploidy in Arabidopsis RT thaliana."; RL Planta 216:604-613(2003). RN [15] RP FUNCTION, AND INTERACTION WITH CDKA-1 AND CYCD3-1. RX PubMed=12566574; DOI=10.1105/tpc.008342; RA Schnittger A., Weinl C., Bouyer D., Schoebinger U., Huelskamp M.; RT "Misexpression of the cyclin-dependent kinase inhibitor ICK1/KRP1 in RT single-celled Arabidopsis trichomes reduces endoreduplication and cell RT size and induces cell death."; RL Plant Cell 15:303-315(2003). RN [16] RP FUNCTION, AND SUBCELLULAR LOCATION. RX PubMed=12904210; DOI=10.1046/j.1365-313X.2003.01821.x; RA Zhou Y., Li G., Brandizzi F., Fowke L.C., Wang H.; RT "The plant cyclin-dependent kinase inhibitor ICK1 has distinct RT functional domains for in vivo kinase inhibition, protein instability RT and nuclear localization."; RL Plant J. 35:476-489(2003). RN [17] RP FUNCTION, AND SUBCELLULAR LOCATION. RX PubMed=15749764; DOI=10.1105/tpc.104.030486; RA Weinl C., Marquardt S., Kuijt S.J.H., Nowack M.K., Jakoby M.J., RA Huelskamp M., Schnittger A.; RT "Novel functions of plant cyclin-dependent kinase inhibitors, RT ICK1/KRP1, can act non-cell-autonomously and inhibit entry into RT mitosis."; RL Plant Cell 17:1704-1722(2005). RN [18] RP FUNCTION. RX PubMed=16376885; DOI=10.1016/j.febslet.2005.12.018; RA Nakai T., Kato K., Shinmyo A., Sekine M.; RT "Arabidopsis KRPs have distinct inhibitory activity toward cyclin D2- RT associated kinases, including plant-specific B-type cyclin-dependent RT kinase."; RL FEBS Lett. 580:336-340(2006). RN [19] RP SUBCELLULAR LOCATION, INTERACTION WITH CDKA-1, AND MUTAGENESIS OF RP LYS-84 AND LYS-86. RX PubMed=16845478; DOI=10.1007/s11103-006-9019-9; RA Zhou Y., Niu H., Brandizzi F., Fowke L.C., Wang H.; RT "Molecular control of nuclear and subnuclear targeting of the plant RT CDK inhibitor ICK1 and ICK1-mediated nuclear transport of CDKA."; RL Plant Mol. Biol. 62:261-278(2006). RN [20] RP FUNCTION, SUBCELLULAR LOCATION, AND MUTAGENESIS OF ARG-80; ARG-81; RP LYS-84 AND LYS-86. RX PubMed=16766674; DOI=10.1104/pp.106.081406; RA Jakoby M.J., Weinl C., Pusch S., Kuijt S.J.H., Merkle T., RA Dissmeyer N., Schnittger A.; RT "Analysis of the subcellular localization, function, and proteolytic RT control of the Arabidopsis cyclin-dependent kinase inhibitor RT ICK1/KRP1."; RL Plant Physiol. 141:1293-1305(2006). RN [21] RP FUNCTION, AND SUBCELLULAR LOCATION. RX PubMed=17253089; DOI=10.1007/s00299-006-0294-3; RA Bird D.A., Buruiana M.M., Zhou Y., Fowke L.C., Wang H.; RT "Arabidopsis cyclin-dependent kinase inhibitors are nuclear-localized RT and show different localization patterns within the nucleoplasm."; RL Plant Cell Rep. 26:861-872(2007). RN [22] RP TISSUE SPECIFICITY, INTERACTION WITH CDKA-1 AND CYCD2-1, RP UBIQUITINATION, AND DISRUPTION PHENOTYPE. RX PubMed=18005227; DOI=10.1111/j.1365-313X.2007.03370.x; RA Ren H., Santner A., del Pozo J.C., Murray J.A., Estelle M.; RT "Degradation of the cyclin-dependent kinase inhibitor KRP1 is RT regulated by two different ubiquitin E3 ligases."; RL Plant J. 53:705-716(2008). CC -!- FUNCTION: Binds and inhibits CYCD2-1/CDKA-1 kinase complex CC activity. Regulates cell division which is crucial for plant CC growth, development and morphogenesis. Functions in turning cells CC from a mitotic to an endoreplicating cell cycle mode. Acts cell- CC and non-cell-autonomously to regulate endoreduplication by CC allowing S phase progression, but blocking entry into mitosis. CC Keeps on the one hand the plant cell cycle locally controlled, and CC on the other hand provides a possibility of linking cell cycle CC control in single cells with the supracellular organization of a CC tissue or an organ. May target specifically CDKA-1. CC -!- SUBUNIT: Specifically interacts with CDKA-1, but not with CDKB1-1. CC Interacts with CYCD2-1 and CYCD3-1. CC -!- INTERACTION: CC P24100:CDKA-1; NbExp=10; IntAct=EBI-1636730, EBI-371713; CC -!- SUBCELLULAR LOCATION: Nucleus, nucleoplasm. Note=Distributed in a CC ponctuate pattern and in heterochromatic chromocenters. CC -!- TISSUE SPECIFICITY: Expressed at low levels in roots, stems, CC leaves and flowers. CC -!- DEVELOPMENTAL STAGE: Highly expressed in root pericycle and cell CC suspension culture during cell cycle arrest. Expressed early in CC the G1 phase, disappears quickly and starts to increase again to CC reach a peak before mitosis. CC -!- INDUCTION: By abscisic acid (ABA). CC -!- PTM: Ubiquitinated independently by RKP and SCF (SKP1-CUL1- CC FBL5/SKP2B) protein ligase complex, leading to proteasomal CC degradation. CC -!- DISRUPTION PHENOTYPE: No visible phenotype. CC -!- MISCELLANEOUS: Plants overexpressing ICK1/KRP1 show altered CC morphology (including flowers) with a reduction in plant size, CC cell numbers, pollen germination, seed production and CDK kinase CC activity. Trichome cells misexpressing ICK1/KRP1 show reduced CC endoreduplication and cell size, and undergo cell death. ICK1/KRP1 CC degradation is regulated by the AXR1-dependent RUB conjugation CC pathway that is required for the function of the SCF complexes. CC -!- SIMILARITY: Belongs to the CDI family. ICK/KRP subfamily. CC -!- SEQUENCE CAUTION: CC Sequence=BAD43255.1; Type=Frameshift; Positions=157; CC Sequence=BAD44338.1; Type=Frameshift; Positions=157; CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; U94772; AAC49698.1; -; mRNA. DR EMBL; AF079587; AAC34660.1; -; Genomic_DNA. DR EMBL; AC003040; AAC23758.1; -; Genomic_DNA. DR EMBL; CP002685; AEC07454.1; -; Genomic_DNA. DR EMBL; AK175492; BAD43255.1; ALT_FRAME; mRNA. DR EMBL; AK176575; BAD44338.1; ALT_FRAME; mRNA. DR EMBL; BT028986; ABI93895.1; -; mRNA. DR EMBL; AY085749; AAM62967.1; -; mRNA. DR IPI; IPI00548875; -. DR PIR; T01132; T01132. DR RefSeq; NP_179924.1; NM_127907.3. DR UniGene; At.24577; -. DR ProteinModelPortal; Q67Y93; -. DR SMR; Q67Y93; 147-191. DR IntAct; Q67Y93; 6. DR STRING; Q67Y93; -. DR EnsemblPlants; AT2G23430; AT2G23430; AT2G23430. DR GeneID; 816875; -. DR GenomeReviews; CT485783_GR; AT2G23430. DR KEGG; ath:AT2G23430; -. DR GeneFarm; 3408; 105. DR TAIR; At2g23430; -. DR eggNOG; NOG273654; -. DR HOGENOM; HOG000113262; -. DR InParanoid; Q67Y93; -. DR OMA; RSRRIVY; -. DR ProtClustDB; CLSN2913303; -. DR Genevestigator; Q67Y93; -. DR GO; GO:0005654; C:nucleoplasm; IEA:UniProtKB-SubCell. DR GO; GO:0005634; C:nucleus; IDA:TAIR. DR GO; GO:0004861; F:cyclin-dependent protein kinase inhibitor activity; ISS:TAIR. DR GO; GO:0007050; P:cell cycle arrest; IEA:InterPro. DR GO; GO:0000082; P:G1/S transition of mitotic cell cycle; TAS:TAIR. DR GO; GO:0010311; P:lateral root formation; IMP:TAIR. DR GO; GO:0048366; P:leaf development; IMP:TAIR. DR GO; GO:0045736; P:negative regulation of cyclin-dependent protein kinase activity; IDA:TAIR. DR GO; GO:0045740; P:positive regulation of DNA replication; IMP:TAIR. DR GO; GO:0009737; P:response to abscisic acid stimulus; IEP:TAIR. DR InterPro; IPR003175; CDI. DR InterPro; IPR016701; CDK_inhib_pln. DR PANTHER; PTHR10265; PTHR10265; 1. DR Pfam; PF02234; CDI; 1. DR PIRSF; PIRSF017811; CDK_inhib_pln; 1. PE 1: Evidence at protein level; KW Cell cycle; Complete proteome; Nucleus; Protein kinase inhibitor; KW Reference proteome; Ubl conjugation. FT CHAIN 1 191 Cyclin-dependent kinase inhibitor 1. FT /FTId=PRO_0000294087. FT REGION 162 191 Required for inhibitory function and FT interaction with CDK kinase complexes. FT MUTAGEN 80 80 R->A: Slightly affects transport to FT nucleus; when associated with A-81. FT MUTAGEN 81 81 R->A: Slightly affects transport to FT nucleus; when associated with A-80. FT MUTAGEN 84 84 K->A: Affects transport to nucleus, but FT does not abolish it; when associated with FT A-86. FT MUTAGEN 86 86 K->A: Affects transport to nucleus, but FT does not abolish it; when associated with FT A-84. FT CONFLICT 88 88 F -> C (in Ref. 1; AAC49698). FT CONFLICT 191 191 E -> Q (in Ref. 7; AAM62967). SQ SEQUENCE 191 AA; 22283 MW; 0477A91E277C46B2 CRC64; MVRKYRKAKG IVEAGVSSTY MQLRSRRIVY VRSEKSSSVS VVGDNGVSSS CSGSNEYKKK ELIHLEEEDK DGDTETSTYR RGTKRKLFEN LREEEKEELS KSMENYSSEF ESAVKESLDC CCSGRKTMEE TVTAEEEEKA KLMTEMPTES EIEDFFVEAE KQLKEKFKKK YNFDFEKEKP LEGRYEWVKL E //