ID ATG9B_HUMAN Reviewed; 924 AA. AC Q674R7; A1A5D3; Q6JRW5; Q8N8I8; DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot. DT 11-OCT-2004, sequence version 1. DT 18-MAR-2008, entry version 30. DE Autophagy-related protein 9B (APG9-like 2) (Nitric oxide synthase 3- DE overlapping antisense gene protein) (Protein sONE). GN Name=ATG9B; Synonyms=APG9L2, NOS3AS; OS Homo sapiens (Human). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; OC Catarrhini; Hominidae; Homo. OX NCBI_TaxID=9606; RN [1] RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), AND TISSUE SPECIFICITY. RC TISSUE=Testis; RX PubMed=15234981; DOI=10.1074/jbc.M400271200; RA Robb G.B., Carson A.R., Tai S.C., Fish J.E., Singh S., Yamada T., RA Scherer S.W., Nakabayashi K., Marsden P.A.; RT "Post-transcriptional regulation of endothelial nitric-oxide synthase RT by an overlapping antisense mRNA transcript."; RL J. Biol. Chem. 279:37982-37996(2004). RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). RC TISSUE=Placenta; RX PubMed=14702039; DOI=10.1038/ng1285; RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S., RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., RA Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., RA Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., RA Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., RA Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., RA Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., RA Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., RA Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., RA Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., RA Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., RA Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., RA Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., RA Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., RA Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., RA Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., RA Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., RA Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., RA Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., RA Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., RA Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.; RT "Complete sequencing and characterization of 21,243 full-length human RT cDNAs."; RL Nat. Genet. 36:40-45(2004). RN [3] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). RX PubMed=15489334; DOI=10.1101/gr.2596504; RG The MGC Project Team; RT "The status, quality, and expansion of the NIH full-length cDNA RT project: the Mammalian Gene Collection (MGC)."; RL Genome Res. 14:2121-2127(2004). RN [4] RP INDUCTION. RX PubMed=17403686; DOI=10.1074/jbc.M608318200; RA Fish J.E., Matouk C.C., Yeboah E., Bevan S.C., Khan M., Patil K., RA Ohh M., Marsden P.A.; RT "Hypoxia-inducible expression of a natural cis-antisense transcript RT inhibits endothelial nitric-oxide synthase."; RL J. Biol. Chem. 282:15652-15666(2007). CC -!- FUNCTION: Plays a role in autophagy (By similarity). CC -!- SUBCELLULAR LOCATION: Cytoplasmic vesicle, autophagosome membrane; CC Multi-pass membrane protein (By similarity). CC -!- ALTERNATIVE PRODUCTS: CC Event=Alternative splicing; Named isoforms=3; CC Name=1; CC IsoId=Q674R7-1; Sequence=Displayed; CC Name=2; CC IsoId=Q674R7-2; Sequence=VSP_030410, VSP_030413; CC Note=No experimental confirmation available; CC Name=3; CC IsoId=Q674R7-3; Sequence=VSP_030411, VSP_030412; CC Note=No experimental confirmation available; CC -!- TISSUE SPECIFICITY: Highly expressed in placenta (trophoblast CC cells) and pituitary gland. Not expressed in vascular endothelial. CC -!- INDUCTION: By hypoxia, leading to inhibit NOS3 expression. CC -!- MISCELLANEOUS: ATG9B gene is located on the opposite DNA strand of CC the NOS3 gene at chromosome 7q36. The genes are oriented in a CC tail-to-tail configuration and the mRNAs encoding ATG9B and NOS3 CC are complementary for 662 nucleotides. ATG9B transcription may a CC role in NOS3 transcription regulation. CC -!- SIMILARITY: Belongs to the ATG9 family. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AY515311; AAS87212.1; -; mRNA. DR EMBL; AY316116; AAQ86941.1; ALT_INIT; mRNA. DR EMBL; AK096734; BAC04853.1; -; mRNA. DR EMBL; BC128587; AAI28588.1; -; mRNA. DR EMBL; BK004019; DAA05200.1; -; mRNA. DR RefSeq; NP_775952.3; -. DR UniGene; Hs.647092; -. DR Ensembl; ENSG00000181652; Homo sapiens. DR GeneID; 285973; -. DR KEGG; hsa:285973; -. DR HGNC; HGNC:21899; ATG9B. DR PharmGKB; PA134883165; -. DR ArrayExpress; Q674R7; -. DR GO; GO:0000045; P:autophagic vacuole formation; IDA:MGI. DR InterPro; IPR007241; Autophagy_prot_99. DR PANTHER; PTHR13038; APG9; 1. DR Pfam; PF04109; APG9; 1. PE 2: Evidence at transcript level; KW Alternative splicing; Autophagy; Cytoplasmic vesicle; Membrane; KW Transmembrane. FT CHAIN 1 924 Autophagy-related protein 9B. FT /FTId=PRO_0000314867. FT TRANSMEM 277 297 Potential. FT TRANSMEM 439 459 Potential. FT TRANSMEM 527 547 Potential. FT TRANSMEM 552 572 Potential. FT TRANSMEM 625 645 Potential. FT COMPBIAS 22 147 Pro-rich. FT VAR_SEQ 1 514 Missing (in isoform 2). FT /FTId=VSP_030410. FT VAR_SEQ 322 340 EELSSVPWAEVQSRLLALQ -> GKGREDTGMYWRGQPGGL FT D (in isoform 3). FT /FTId=VSP_030411. FT VAR_SEQ 341 924 Missing (in isoform 3). FT /FTId=VSP_030412. FT VAR_SEQ 515 572 RTAAPPAPLRTLLARQLVFFAGALFAALLVLTVYDEDVLAV FT EHVLTAMTALGVTATVA -> MPSYPQPSVLRGSAPARCWS FT SSWSWLPASGWSNCFAQSATSSATGTSRCFTGRPCTSP FT (in isoform 2). FT /FTId=VSP_030413. FT CONFLICT 12 12 R -> K (in Ref. 2; BAC04853). FT CONFLICT 751 751 S -> P (in Ref. 1; AAQ86941). FT CONFLICT 752 752 T -> P (in Ref. 1; AAQ86941). FT CONFLICT 760 760 T -> A (in Ref. 1; AAQ86941). SQ SEQUENCE 924 AA; 101019 MW; 80C242069DF3D91E CRC64; MVSRMGWGGR RRRLGRWGDL GPGSVPLLPM PLPPPPPPSC RGPGGGRISI FSLSPAPHTR SSPSSFSPPT AGPPCSVLQG TGASQSCHSA LPIPATPPTQ AQPAMTPASA SPSWGSHSTP PLAPATPTPS QQCPQDSPGL RVGPLIPEQD YERLEDCDPE GSQDSPIHGE EQQPLLHVPE GLRGSWHHIQ NLDSFFTKIY SYHQRNGFAC ILLEDVFQLG QFIFIVTFTT FLLRCVDYNV LFANQPSNHT RPGPFHSKVT LSDAILPSAQ CAERIRSSPL LVLLLVLAAG FWLVQLLRSV CNLFSYWDIQ VFYREALHIP PEELSSVPWA EVQSRLLALQ RSGGLCVQPR PLTELDIHHR ILRYTNYQVA LANKGLLPAR CPLPWGGSAA FLSRGLALNV DLLLFRGPFS LFRGGWELPH AYKRSDQRGA LAARWGRTVL LLAALNLALS PLVLAWQVLH VFYSHVELLR REPGALGARG WSRLARLQLR HFNELPHELR ARLARAYRPA AAFLRTAAPP APLRTLLARQ LVFFAGALFA ALLVLTVYDE DVLAVEHVLT AMTALGVTAT VARSFIPEEQ CQGRAPQLLL QTALAHMHYL PEEPGPGGRD RAYRQMAQLL QYRAVSLLEE LLSPLLTPLF LLFWFRPRAL EIIDFFHHFT VDVAGVGDIC SFALMDVKRH GHPQWLSAGQ TEASLSQRAE DGKTELSLMR FSLAHPLWRP PGHSSKFLGH LWGRVQQDAA AWGATSARGP STPGVLSNCT SPLPEAFLAN LFVHPLLPPR DLSPTAPCPA AATASLLASI SRIAQDPSSV SPGGTGGQKL AQLPELASAE MSLHVIYLHQ LHQQQQQQEP WGEAAASILS RPCSSPSQPP SPDEEKPSWS SDGSSPASSP RQQWGTQKAR NLFPGGFQVT TDTQKEPDRA SCTD //