ID SIAT1_MOUSE Reviewed; 403 AA. AC Q64685; DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot. DT 01-NOV-1996, sequence version 1. DT 11-JAN-2011, entry version 82. DE RecName: Full=Beta-galactoside alpha-2,6-sialyltransferase 1; DE Short=Alpha 2,6-ST 1; DE EC=2.4.99.1; DE AltName: Full=CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,6-sialyltransferase 1; DE AltName: Full=ST6Gal I; DE Short=ST6GalI; DE AltName: Full=Sialyltransferase 1; GN Name=St6gal1; Synonyms=Siat1; OS Mus musculus (Mouse). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; OC Muroidea; Muridae; Murinae; Mus; Mus. OX NCBI_TaxID=10090; RN [1] RP NUCLEOTIDE SEQUENCE [MRNA]. RC TISSUE=Brain, and Liver; RX MEDLINE=94363344; PubMed=8081843; DOI=10.1016/S0968-0896(00)82111-2; RA Hamamoto T., Kawasaki M., Kurosawa N., Nakaoka T., Lee Y.-C., RA Tsuji S.; RT "Two step single primer mediated polymerase chain reaction. RT Application to cloning of putative mouse, beta-galactoside alpha 2,6- RT sialyltransferase cDNA."; RL Bioorg. Med. Chem. 1:141-145(1993). CC -!- FUNCTION: Transfers sialic acid from the donor of substrate CMP- CC sialic acid to galactose containing acceptor substrates. CC -!- CATALYTIC ACTIVITY: CMP-N-acetylneuraminate + beta-D-galactosyl- CC 1,4-N-acetyl-beta-D-glucosamine = CMP + alpha-N-acetylneuraminyl- CC 2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine. CC -!- PATHWAY: Protein modification; protein glycosylation. CC -!- SUBCELLULAR LOCATION: Golgi apparatus, Golgi stack membrane; CC Single-pass type II membrane protein. Secreted. Note=Membrane- CC bound form in trans cisternae of Golgi. Secreted into the body CC fluid. CC -!- PTM: The soluble form derives from the membrane form by CC proteolytic processing. CC -!- SIMILARITY: Belongs to the glycosyltransferase 29 family. CC -!- WEB RESOURCE: Name=Functional Glycomics Gateway - GTase; CC Note=ST6Gal I; CC URL="http://www.functionalglycomics.org/glycomics/molecule/jsp/glycoEnzyme/viewGlycoEnzyme.jsp?gbpId=gt_mou_648"; CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; D16106; BAA03680.1; -; mRNA. DR IPI; IPI00136942; -. DR UniGene; Mm.149029; -. DR ProteinModelPortal; Q64685; -. DR SMR; Q64685; 174-388. DR STRING; Q64685; -. DR CAZy; GT29; Glycosyltransferase Family 29. DR PhosphoSite; Q64685; -. DR PRIDE; Q64685; -. DR Ensembl; ENSMUST00000023601; ENSMUSP00000023601; ENSMUSG00000022885. DR Ensembl; ENSMUST00000115335; ENSMUSP00000110992; ENSMUSG00000022885. DR MGI; MGI:108470; St6gal1. DR eggNOG; roNOG04277; -. DR HOGENOM; HBG717863; -. DR HOVERGEN; HBG052853; -. DR InParanoid; Q64685; -. DR BRENDA; 2.4.99.1; 244. DR ArrayExpress; Q64685; -. DR Bgee; Q64685; -. DR CleanEx; MM_ST6GAL1; -. DR Genevestigator; Q64685; -. DR GermOnline; ENSMUSG00000022885; Mus musculus. DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell. DR GO; GO:0032580; C:Golgi cisterna membrane; IEA:UniProtKB-SubCell. DR GO; GO:0030173; C:integral to Golgi membrane; IEA:InterPro. DR GO; GO:0003835; F:beta-galactoside alpha-2,6-sialyltransferase activity; IEA:EC. DR GO; GO:0006486; P:protein glycosylation; IEA:InterPro. DR InterPro; IPR001675; Glyco_trans_29. DR InterPro; IPR012163; Sialyl_trans. DR Pfam; PF00777; Glyco_transf_29; 1. DR PIRSF; PIRSF005557; Sialyl_trans; 1. PE 2: Evidence at transcript level; KW Disulfide bond; Glycoprotein; Glycosyltransferase; Golgi apparatus; KW Membrane; Phosphoprotein; Secreted; Signal-anchor; Transferase; KW Transmembrane; Transmembrane helix. FT CHAIN 1 403 Beta-galactoside alpha-2,6- FT sialyltransferase 1. FT /FTId=PRO_0000149250. FT TOPO_DOM 1 9 Cytoplasmic (Potential). FT TRANSMEM 10 26 Helical; Signal-anchor for type II FT membrane protein; (Potential). FT TOPO_DOM 27 403 Lumenal (Potential). FT MOD_RES 366 366 Phosphotyrosine (By similarity). FT CARBOHYD 146 146 N-linked (GlcNAc...) (Potential). FT CARBOHYD 158 158 N-linked (GlcNAc...) (Potential). FT DISULFID 181 332 By similarity. SQ SEQUENCE 403 AA; 46407 MW; ECA8D0603A5A847B CRC64; MIHTNLKRKF SCFVLVFLLF AIICVWKKGS DYEALTLQAK VFQMPKSQEK VAVGPAPQAV FSNSKQDPKE GVQILSYPRV TAKVKPQPSL QVWDKDSTYS KLNPRLLKIW RNYLNMNKYK VSYKGPGPGV RFSVEGLRCH LRDHVNVSMI EATDSPFNTT EWEGYLPKET FRTKAGPCTK CAVVSSAGSL KNSQLGREID NHDAVLRFNG APTDNFQQDV GTKTTIRLVN SQLVTTEKRF LKDSLYTEGI LILWDPSVYH ADIPQWYQKP DYNFFETYKS YRRLHPSQPF YILKPQMPWE LWDIIQEISP DLIQPNPPSS GMLGIIIMMT LCDQVDIYEF LPSKRKTDVC YYHQKFFDSA CTMGAYHPLL FEKNMVKHLN EGTDEDIYLF GKATLSGFRN NRC //