ID SIAT1_MOUSE Reviewed; 403 AA. AC Q64685; DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot. DT 01-NOV-1996, sequence version 1. DT 23-JAN-2007, entry version 51. DE CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,6-sialyltransferase DE (EC 2.4.99.1) (Beta-galactoside alpha-2,6-sialyltransferase) (Alpha DE 2,6-ST) (Sialyltransferase 1) (ST6Gal I). GN Name=St6gal1; Synonyms=Siat1; OS Mus musculus (Mouse). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; OC Muroidea; Muridae; Murinae; Mus. OX NCBI_TaxID=10090; RN [1] RP NUCLEOTIDE SEQUENCE [MRNA]. RC TISSUE=Brain, and Liver; RX MEDLINE=94363344; PubMed=8081843; DOI=10.1016/S0968-0896(00)82111-2; RA Hamamoto T., Kawasaki M., Kurosawa N., Nakaoka T., Lee Y.-C., RA Tsuji S.; RT "Two step single primer mediated polymerase chain reaction. RT Application to cloning of putative mouse, beta-galactoside alpha 2,6- RT sialyltransferase cDNA."; RL Bioorg. Med. Chem. 1:141-145(1993). CC -!- FUNCTION: Transfers sialic acid from the donor of substrate CMP- CC sialic acid to galactose containing acceptor substrates. CC -!- CATALYTIC ACTIVITY: CMP-N-acetylneuraminate + beta-D-galactosyl- CC 1,4-N-acetyl-beta-D-glucosamine = CMP + alpha-N-acetylneuraminyl- CC 2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine. CC -!- PATHWAY: Glycosylation. CC -!- SUBCELLULAR LOCATION: Golgi apparatus; Golgi stack; Golgi stack CC membrane; single-pass type II membrane protein. Secreted protein. CC Note=Membrane-bound form in trans cisternae of Golgi. Secreted CC into the body fluid. CC -!- PTM: The soluble form derives from the membrane form by CC proteolytic processing. CC -!- SIMILARITY: Belongs to the glycosyltransferase 29 family. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; D16106; BAA03680.1; -; mRNA. DR Ensembl; ENSMUSG00000022885; Mus musculus. DR KEGG; mmu:20440; -. DR MGI; MGI:108470; St6gal1. DR ArrayExpress; Q64685; -. DR GermOnline; ENSMUSG00000022885; Mus musculus. DR GO; GO:0008373; F:sialyltransferase activity; IDA:MGI. DR InterPro; IPR001675; Glyco_trans_29. DR InterPro; IPR012163; Sialyl_trans. DR Pfam; PF00777; Glyco_transf_29; 1. DR PIRSF; PIRSF005557; Sialyl_trans; 1. KW Glycoprotein; Glycosyltransferase; Golgi apparatus; Membrane; KW Signal-anchor; Transferase; Transmembrane. FT CHAIN 1 403 CMP-N-acetylneuraminate-beta- FT galactosamide-alpha-2,6- FT sialyltransferase. FT /FTId=PRO_0000149250. FT TOPO_DOM 1 9 Cytoplasmic (Potential). FT TRANSMEM 10 26 Signal-anchor for type II membrane FT protein (Potential). FT TOPO_DOM 27 403 Lumenal (Potential). FT CARBOHYD 146 146 N-linked (GlcNAc...) (Potential). FT CARBOHYD 158 158 N-linked (GlcNAc...) (Potential). FT DISULFID 181 332 By similarity. SQ SEQUENCE 403 AA; 46407 MW; ECA8D0603A5A847B CRC64; MIHTNLKRKF SCFVLVFLLF AIICVWKKGS DYEALTLQAK VFQMPKSQEK VAVGPAPQAV FSNSKQDPKE GVQILSYPRV TAKVKPQPSL QVWDKDSTYS KLNPRLLKIW RNYLNMNKYK VSYKGPGPGV RFSVEGLRCH LRDHVNVSMI EATDSPFNTT EWEGYLPKET FRTKAGPCTK CAVVSSAGSL KNSQLGREID NHDAVLRFNG APTDNFQQDV GTKTTIRLVN SQLVTTEKRF LKDSLYTEGI LILWDPSVYH ADIPQWYQKP DYNFFETYKS YRRLHPSQPF YILKPQMPWE LWDIIQEISP DLIQPNPPSS GMLGIIIMMT LCDQVDIYEF LPSKRKTDVC YYHQKFFDSA CTMGAYHPLL FEKNMVKHLN EGTDEDIYLF GKATLSGFRN NRC //