ID   ZKSC2_HUMAN             Reviewed;         967 AA.
AC   Q63HK3; A1L3B4; Q6ZN77;
DT   02-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT   18-MAY-2010, sequence version 2.
DT   14-DEC-2022, entry version 148.
DE   RecName: Full=Zinc finger protein with KRAB and SCAN domains 2;
DE   AltName: Full=Zinc finger protein 694;
GN   Name=ZKSCAN2; Synonyms=ZNF694;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC   TISSUE=Thymus;
RX   PubMed=14702039; DOI=10.1038/ng1285;
RA   Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA   Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA   Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA   Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA   Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA   Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA   Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA   Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA   Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA   Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA   Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA   Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA   Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA   Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA   Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA   Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA   Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA   Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA   Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA   Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA   Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA   Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA   Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA   Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA   Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA   Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA   Isogai T., Sugano S.;
RT   "Complete sequencing and characterization of 21,243 full-length human
RT   cDNAs.";
RL   Nat. Genet. 36:40-45(2004).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC   TISSUE=Uterine endothelium;
RX   PubMed=17974005; DOI=10.1186/1471-2164-8-399;
RA   Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U.,
RA   Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D.,
RA   Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A.,
RA   Wiemann S., Schupp I.;
RT   "The full-ORF clone resource of the German cDNA consortium.";
RL   BMC Genomics 8:399-399(2007).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=15616553; DOI=10.1038/nature03187;
RA   Martin J., Han C., Gordon L.A., Terry A., Prabhakar S., She X., Xie G.,
RA   Hellsten U., Chan Y.M., Altherr M., Couronne O., Aerts A., Bajorek E.,
RA   Black S., Blumer H., Branscomb E., Brown N.C., Bruno W.J., Buckingham J.M.,
RA   Callen D.F., Campbell C.S., Campbell M.L., Campbell E.W., Caoile C.,
RA   Challacombe J.F., Chasteen L.A., Chertkov O., Chi H.C., Christensen M.,
RA   Clark L.M., Cohn J.D., Denys M., Detter J.C., Dickson M.,
RA   Dimitrijevic-Bussod M., Escobar J., Fawcett J.J., Flowers D., Fotopulos D.,
RA   Glavina T., Gomez M., Gonzales E., Goodstein D., Goodwin L.A., Grady D.L.,
RA   Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Hildebrand C.E.,
RA   Huang W., Israni S., Jett J., Jewett P.B., Kadner K., Kimball H.,
RA   Kobayashi A., Krawczyk M.-C., Leyba T., Longmire J.L., Lopez F., Lou Y.,
RA   Lowry S., Ludeman T., Manohar C.F., Mark G.A., McMurray K.L., Meincke L.J.,
RA   Morgan J., Moyzis R.K., Mundt M.O., Munk A.C., Nandkeshwar R.D.,
RA   Pitluck S., Pollard M., Predki P., Parson-Quintana B., Ramirez L., Rash S.,
RA   Retterer J., Ricke D.O., Robinson D.L., Rodriguez A., Salamov A.,
RA   Saunders E.H., Scott D., Shough T., Stallings R.L., Stalvey M.,
RA   Sutherland R.D., Tapia R., Tesmer J.G., Thayer N., Thompson L.S., Tice H.,
RA   Torney D.C., Tran-Gyamfi M., Tsai M., Ulanovsky L.E., Ustaszewska A.,
RA   Vo N., White P.S., Williams A.L., Wills P.L., Wu J.-R., Wu K., Yang J.,
RA   DeJong P., Bruce D., Doggett N.A., Deaven L., Schmutz J., Grimwood J.,
RA   Richardson P., Rokhsar D.S., Eichler E.E., Gilna P., Lucas S.M.,
RA   Myers R.M., Rubin E.M., Pennacchio L.A.;
RT   "The sequence and analysis of duplication-rich human chromosome 16.";
RL   Nature 432:988-994(2004).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), AND VARIANT ASP-615.
RC   TISSUE=Brain;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [5]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-591 AND SER-600, AND
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Cervix carcinoma;
RX   PubMed=18669648; DOI=10.1073/pnas.0805139105;
RA   Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,
RA   Elledge S.J., Gygi S.P.;
RT   "A quantitative atlas of mitotic phosphorylation.";
RL   Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
RN   [6]
RP   SUMOYLATION [LARGE SCALE ANALYSIS] AT LYS-22; LYS-242; LYS-259; LYS-277;
RP   LYS-337; LYS-482; LYS-529; LYS-734; LYS-745 AND LYS-752, AND IDENTIFICATION
RP   BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=28112733; DOI=10.1038/nsmb.3366;
RA   Hendriks I.A., Lyon D., Young C., Jensen L.J., Vertegaal A.C.,
RA   Nielsen M.L.;
RT   "Site-specific mapping of the human SUMO proteome reveals co-modification
RT   with phosphorylation.";
RL   Nat. Struct. Mol. Biol. 24:325-336(2017).
CC   -!- FUNCTION: May be involved in transcriptional regulation.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=Q63HK3-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q63HK3-2; Sequence=VSP_018189, VSP_018190;
CC   -!- SIMILARITY: Belongs to the krueppel C2H2-type zinc-finger protein
CC       family. {ECO:0000305}.
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DR   EMBL; AK131342; BAD18498.1; -; mRNA.
DR   EMBL; BX648785; CAH56131.1; -; mRNA.
DR   EMBL; AC008741; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; BC130007; AAI30008.1; -; mRNA.
DR   EMBL; BC151139; AAI51140.1; -; mRNA.
DR   CCDS; CCDS32410.1; -. [Q63HK3-1]
DR   RefSeq; NP_001012999.3; NM_001012981.4. [Q63HK3-1]
DR   AlphaFoldDB; Q63HK3; -.
DR   SMR; Q63HK3; -.
DR   BioGRID; 131170; 7.
DR   STRING; 9606.ENSP00000331626; -.
DR   iPTMnet; Q63HK3; -.
DR   PhosphoSitePlus; Q63HK3; -.
DR   BioMuta; ZKSCAN2; -.
DR   DMDM; 296453036; -.
DR   jPOST; Q63HK3; -.
DR   MassIVE; Q63HK3; -.
DR   MaxQB; Q63HK3; -.
DR   PaxDb; Q63HK3; -.
DR   PeptideAtlas; Q63HK3; -.
DR   ProteomicsDB; 65876; -. [Q63HK3-1]
DR   ProteomicsDB; 65877; -. [Q63HK3-2]
DR   ABCD; Q63HK3; 5 sequenced antibodies.
DR   Antibodypedia; 59128; 57 antibodies from 12 providers.
DR   DNASU; 342357; -.
DR   Ensembl; ENST00000328086.12; ENSP00000331626.7; ENSG00000155592.16. [Q63HK3-1]
DR   GeneID; 342357; -.
DR   KEGG; hsa:342357; -.
DR   MANE-Select; ENST00000328086.12; ENSP00000331626.7; NM_001012981.5; NP_001012999.3.
DR   UCSC; uc002dod.5; human. [Q63HK3-1]
DR   AGR; HGNC:25677; -.
DR   CTD; 342357; -.
DR   GeneCards; ZKSCAN2; -.
DR   HGNC; HGNC:25677; ZKSCAN2.
DR   HPA; ENSG00000155592; Low tissue specificity.
DR   neXtProt; NX_Q63HK3; -.
DR   PharmGKB; PA162409748; -.
DR   VEuPathDB; HostDB:ENSG00000155592; -.
DR   eggNOG; KOG1721; Eukaryota.
DR   GeneTree; ENSGT00940000161884; -.
DR   HOGENOM; CLU_002678_88_0_1; -.
DR   InParanoid; Q63HK3; -.
DR   OMA; NGHMLEP; -.
DR   OrthoDB; 1318335at2759; -.
DR   PhylomeDB; Q63HK3; -.
DR   TreeFam; TF336839; -.
DR   PathwayCommons; Q63HK3; -.
DR   BioGRID-ORCS; 342357; 16 hits in 1106 CRISPR screens.
DR   GenomeRNAi; 342357; -.
DR   Pharos; Q63HK3; Tdark.
DR   PRO; PR:Q63HK3; -.
DR   Proteomes; UP000005640; Chromosome 16.
DR   RNAct; Q63HK3; protein.
DR   Bgee; ENSG00000155592; Expressed in cortical plate and 150 other tissues.
DR   ExpressionAtlas; Q63HK3; baseline and differential.
DR   Genevisible; Q63HK3; HS.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   CDD; cd07765; KRAB_A-box; 1.
DR   CDD; cd07936; SCAN; 1.
DR   Gene3D; 1.10.4020.10; -; 1.
DR   InterPro; IPR001909; KRAB.
DR   InterPro; IPR036051; KRAB_dom_sf.
DR   InterPro; IPR044822; Myb_DNA-bind_4.
DR   InterPro; IPR038765; Papain-like_cys_pep_sf.
DR   InterPro; IPR003309; SCAN_dom.
DR   InterPro; IPR038269; SCAN_sf.
DR   InterPro; IPR036236; Znf_C2H2_sf.
DR   InterPro; IPR013087; Znf_C2H2_type.
DR   Pfam; PF01352; KRAB; 1.
DR   Pfam; PF13837; Myb_DNA-bind_4; 2.
DR   Pfam; PF02023; SCAN; 1.
DR   Pfam; PF00096; zf-C2H2; 4.
DR   SMART; SM00349; KRAB; 1.
DR   SMART; SM00431; SCAN; 1.
DR   SMART; SM00355; ZnF_C2H2; 6.
DR   SUPFAM; SSF57667; beta-beta-alpha zinc fingers; 3.
DR   SUPFAM; SSF54001; Cysteine proteinases; 1.
DR   SUPFAM; SSF109640; KRAB domain (Kruppel-associated box); 1.
DR   PROSITE; PS50805; KRAB; 1.
DR   PROSITE; PS50804; SCAN_BOX; 1.
DR   PROSITE; PS00028; ZINC_FINGER_C2H2_1; 6.
DR   PROSITE; PS50157; ZINC_FINGER_C2H2_2; 6.
PE   1: Evidence at protein level;
KW   Alternative splicing; DNA-binding; Isopeptide bond; Metal-binding; Nucleus;
KW   Phosphoprotein; Reference proteome; Repeat; Transcription;
KW   Transcription regulation; Ubl conjugation; Zinc; Zinc-finger.
FT   CHAIN           1..967
FT                   /note="Zinc finger protein with KRAB and SCAN domains 2"
FT                   /id="PRO_0000234016"
FT   DOMAIN          45..127
FT                   /note="SCAN box"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00187"
FT   DOMAIN          229..300
FT                   /note="KRAB"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00119"
FT   ZN_FING         775..797
FT                   /note="C2H2-type 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT   ZN_FING         803..825
FT                   /note="C2H2-type 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT   ZN_FING         831..853
FT                   /note="C2H2-type 3"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT   ZN_FING         859..881
FT                   /note="C2H2-type 4"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT   ZN_FING         887..909
FT                   /note="C2H2-type 5"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT   ZN_FING         915..937
FT                   /note="C2H2-type 6"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT   REGION          150..205
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          586..626
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          941..967
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        172..189
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         591
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:18669648"
FT   MOD_RES         600
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:18669648"
FT   CROSSLNK        22
FT                   /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT                   G-Cter in SUMO2)"
FT                   /evidence="ECO:0007744|PubMed:28112733"
FT   CROSSLNK        242
FT                   /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT                   G-Cter in SUMO2)"
FT                   /evidence="ECO:0007744|PubMed:28112733"
FT   CROSSLNK        259
FT                   /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT                   G-Cter in SUMO2)"
FT                   /evidence="ECO:0007744|PubMed:28112733"
FT   CROSSLNK        277
FT                   /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT                   G-Cter in SUMO2)"
FT                   /evidence="ECO:0007744|PubMed:28112733"
FT   CROSSLNK        337
FT                   /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT                   G-Cter in SUMO2)"
FT                   /evidence="ECO:0007744|PubMed:28112733"
FT   CROSSLNK        482
FT                   /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT                   G-Cter in SUMO2)"
FT                   /evidence="ECO:0007744|PubMed:28112733"
FT   CROSSLNK        529
FT                   /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT                   G-Cter in SUMO2)"
FT                   /evidence="ECO:0007744|PubMed:28112733"
FT   CROSSLNK        734
FT                   /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT                   G-Cter in SUMO2)"
FT                   /evidence="ECO:0007744|PubMed:28112733"
FT   CROSSLNK        745
FT                   /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT                   G-Cter in SUMO2)"
FT                   /evidence="ECO:0007744|PubMed:28112733"
FT   CROSSLNK        752
FT                   /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT                   G-Cter in SUMO2)"
FT                   /evidence="ECO:0007744|PubMed:28112733"
FT   VAR_SEQ         498..505
FT                   /note="VHWGYEET -> KNCALFLW (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:14702039"
FT                   /id="VSP_018189"
FT   VAR_SEQ         506..967
FT                   /note="Missing (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:14702039"
FT                   /id="VSP_018190"
FT   VARIANT         253
FT                   /note="L -> F (in dbSNP:rs2112811)"
FT                   /id="VAR_033597"
FT   VARIANT         615
FT                   /note="E -> D (in dbSNP:rs8059494)"
FT                   /evidence="ECO:0000269|PubMed:15489334"
FT                   /id="VAR_033598"
FT   VARIANT         947
FT                   /note="P -> S (in dbSNP:rs7197424)"
FT                   /id="VAR_057460"
FT   CONFLICT        667
FT                   /note="S -> G (in Ref. 2; CAH56131)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   967 AA;  110941 MW;  6742FECD4D5D5300 CRC64;
     MAVALDSQID APLEVEGCLI MKVEKDPEWA SEPILEGSDS SETFRKCFRQ FCYEDVTGPH
     EAFSKLWELC CRWLKPEMRS KEQILELLVI EQFLTILPEK IQAWAQKQCP QSGEEAVALV
     VHLEKETGRL RQQVSSPVHR EKHSPLGAAW EVADFQPEQV ETQPRAVSRE EPGSLHSGHQ
     EQLNRKRERR PLPKNARPSP WVPALADEWN TLDQEVTTTR LPAGSQEPVK DVHVARGFSY
     RKSVHQIPAQ RDLYRDFRKE NVGNVVSLGS AVSTSNKITR LEQRKEPWTL GLHSSNKRSI
     LRSNYVKEKS VHAIQVPARS AGKTWREQQQ WGLEDEKIAG VHWSYEETKT FLAILKESRF
     YETLQACPRN SQVYGAVAEW LRECGFLRTP EQCRTKFKSL QKSYRKVRNG HMLEPCAFFE
     DMDALLNPAA RAPSTDKPKE MIPVPRLKRI AISAKEHISL VEEEEAAEDS DDDEIGIEFI
     RKSEIHGAPV LFQNLSGVHW GYEETKTFLD ILRETRFYEA LQACHRKSKL YGAVAEQLRE
     CGFLRTPEQC RTKFKSLQKS YRKVKNGHVL ESCAFYKEMD ALINSRASAP SPSTPEEVPS
     PSRQERGGIE VEPQEPTGWE PEETSQEAVI EDSCSERMSE EEIVQEPEFQ GPPGLLQSPN
     DFEIGSSIKE DPTQIVYKDM EQHRALIEKS KRVVSQSTDP SKYRKRECIS GRQWENLQGI
     RQGKPMSQPR DLGKAVVHQR PFVGKRPYRL LKYGESFGRS TRLMCRMTHH KENPYKCGVC
     GKCFGRSRSL IRHQRIHTGE KPFKCLDCGK SFNDSSNFGA HQRIHTGEKP YRCGECGKCF
     SQSSSLIIHQ RTHTGEKPYQ CGECGKSFTN SSHFSAHRRV HTGENPYKCV DCEKSFNNCT
     RFREHRRIHT GEKPYGCAQC GKRFSKSSVL TKHREVHVRE KPLPHPPSLY CPENPHKGKT
     DEFRKTF
//