ID Q5U924_PEPDI Unreviewed; 408 AA. AC Q5U924; DT 07-DEC-2004, integrated into UniProtKB/TrEMBL. DT 07-DEC-2004, sequence version 1. DT 09-JUL-2014, entry version 27. DE SubName: Full=2-hydroxyglutaryl-CoA dehydratase; DE SubName: Full=2-hydroxyisocaproyl-CoA dehydratase; GN Name=hadB; ORFNames=BG47_14005; OS Peptoclostridium difficile (Clostridium difficile). OC Bacteria; Firmicutes; Clostridia; Clostridiales; OC Peptostreptococcaceae; Peptoclostridium. OX NCBI_TaxID=1496; RN [1] RP NUCLEOTIDE SEQUENCE. RC STRAIN=DSM 1296; RX PubMed=15654892; DOI=10.1111/j.1742-4658.2004.04498.x; RA Kim J., Darley D., Buckel W.; RT "2-Hydroxyisocaproyl-CoA dehydratase and its activator from RT Clostridium difficile."; RL FEBS J. 272:550-561(2005). RN [2] RP X-RAY CRYSTALLOGRAPHY (1.82 ANGSTROMS) IN COMPLEX WITH IRON-SULFUR RP (4FE-4S) AND SULFATE. RX PubMed=21366233; DOI=10.1021/ja1076537; RA Knauer S.H., Buckel W., Dobbek H.; RT "Structural basis for reductive radical formation and electron RT recycling in (R)-2-hydroxyisocaproyl-CoA dehydratase."; RL J. Am. Chem. Soc. 133:4342-4347(2011). RN [3] RP NUCLEOTIDE SEQUENCE. RC STRAIN=SG12; RA Mooyottu S., Flock G., Upadhyaya I., Venkitanarayanan K.; RT "Prevalence of Clostridium difficile in Retail Meat and RT Characterization of a Multidrug Resistant C. difficile Isolated from RT Ground Pork in Connecticut."; RL Submitted (FEB-2014) to the EMBL/GenBank/DDBJ databases. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AY772816; AAV40819.1; -; Genomic_DNA. DR EMBL; JFAF01000058; EZR27309.1; -; Genomic_DNA. DR PDB; 3O3M; X-ray; 1.82 A; A/C=1-408. DR PDB; 3O3N; X-ray; 2.30 A; A/C=1-408. DR PDB; 3O3O; X-ray; 2.00 A; A=1-408. DR PDBsum; 3O3M; -. DR PDBsum; 3O3N; -. DR PDBsum; 3O3O; -. DR EvolutionaryTrace; Q5U924; -. DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW. DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW. DR InterPro; IPR010327; HGD-D. DR Pfam; PF06050; HGD-D; 1. PE 1: Evidence at protein level; KW 3D-structure; 4Fe-4S; Iron; Iron-sulfur; Metal-binding. FT REGION 55 56 Sulfate binding. FT METAL 84 84 Iron-sulfur (4Fe-4S){EI1,EI2,EI3}. FT METAL 117 117 Iron-sulfur (4Fe-4S){EI1,EI2,EI3}. FT METAL 346 346 Iron-sulfur (4Fe-4S){EI1,EI2,EI3}. SQ SEQUENCE 408 AA; 46334 MW; 702A372CB6796AB5 CRC64; MSEKKEARVV INDLLAEQYA NAFKAKEEGR PVGWSTSVFP QELAEVFDLN VLYPENQAAG VAAKKGSLEL CEIAESKGYS IDLCAYARTN FGLLENGGCE ALDMPAPDFL LCCNNICNQV IKWYENISRE LDIPLIMIDT TFNNEDEVTQ SRIDYIKAQF EEAIKQLEII SGKKFDPKKF EEVMKISAEN GRLWKYSMSL PADSSPSPMN GFDLFTYMAV IVCARGKKET TEAFKLLIEE LEDNMKTGKS SFRGEEKYRI MMEGIPCWPY IGYKMKTLAK FGVNMTGSVY PHAWALQYEV NDLDGMAVAY STMFNNVNLD RMTKYRVDSL VEGKCDGAFY HMNRSCKLMS LIQYEMQRRA AEETGLPYAG FDGDQADPRA FTNAQFETRI QGLVEVMEER KKLNRGEI //