ID SPD2A_HUMAN Reviewed; 1133 AA. AC Q5TCZ1; D3DR98; O43302; Q5TCZ2; Q5TDQ8; DT 20-FEB-2007, integrated into UniProtKB/Swiss-Prot. DT 21-DEC-2004, sequence version 1. DT 19-FEB-2014, entry version 96. DE RecName: Full=SH3 and PX domain-containing protein 2A; DE AltName: Full=Adapter protein TKS5; DE AltName: Full=Five SH3 domain-containing protein; DE AltName: Full=SH3 multiple domains protein 1; DE AltName: Full=Tyrosine kinase substrate with five SH3 domains; GN Name=SH3PXD2A; Synonyms=FISH, KIAA0418, SH3MD1, TKS5; OS Homo sapiens (Human). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; OC Catarrhini; Hominidae; Homo. OX NCBI_TaxID=9606; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). RC TISSUE=Brain; RX PubMed=9455477; DOI=10.1093/dnares/4.5.307; RA Ishikawa K., Nagase T., Nakajima D., Seki N., Ohira M., Miyajima N., RA Tanaka A., Kotani H., Nomura N., Ohara O.; RT "Prediction of the coding sequences of unidentified human genes. VIII. RT 78 new cDNA clones from brain which code for large proteins in RT vitro."; RL DNA Res. 4:307-313(1997). RN [2] RP SEQUENCE REVISION. RA Okazaki N., Kikuno R.F., Nagase T., Ohara O., Koga H.; RL Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases. RN [3] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RX PubMed=15164054; DOI=10.1038/nature02462; RA Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L., RA Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K., RA Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L., RA Taylor A., Battles J., Bird C.P., Ainscough R., Almeida J.P., RA Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J., RA Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., RA Brown J.Y., Burford D.C., Burrill W., Burton J., Cahill P., Camire D., RA Carter N.P., Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., RA Corby N., Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., RA Frankish A., Frankland J.A., Garner P., Garnett J., Gribble S., RA Griffiths C., Grocock R., Gustafson E., Hammond S., Harley J.L., RA Hart E., Heath P.D., Ho T.P., Hopkins B., Horne J., Howden P.J., RA Huckle E., Hynds C., Johnson C., Johnson D., Kana A., Kay M., RA Kimberley A.M., Kershaw J.K., Kokkinaki M., Laird G.K., Lawlor S., RA Lee H.M., Leongamornlert D.A., Laird G., Lloyd C., Lloyd D.M., RA Loveland J., Lovell J., McLaren S., McLay K.E., McMurray A., RA Mashreghi-Mohammadi M., Matthews L., Milne S., Nickerson T., RA Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V., Peck A.I., RA Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A., Ross M.T., RA Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., RA Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., RA Tracey A., Tromans A., Tsolas J., Wall M., Walsh J., Wang H., RA Weinstock K., West A.P., Willey D.L., Whitehead S.L., Wilming L., RA Wray P.W., Young L., Chen Y., Lovering R.C., Moschonas N.K., RA Siebert R., Fechtel K., Bentley D., Durbin R.M., Hubbard T., RA Doucette-Stamm L., Beck S., Smith D.R., Rogers J.; RT "The DNA sequence and comparative analysis of human chromosome 10."; RL Nature 429:375-381(2004). RN [4] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., RA Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., RA Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., RA Hannenhalli S., Turner R., Yooseph S., Lu F., Nusskern D.R., RA Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., RA Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., RA Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., RA Venter J.C.; RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases. RN [5] RP FUNCTION, SUBCELLULAR LOCATION, DOMAIN, INTERACTION WITH ADAM12; RP ADAM15 AND ADAM19, AND MUTAGENESIS OF ARG-42 AND ARG-93. RX PubMed=12615925; DOI=10.1074/jbc.M300267200; RA Abram C.L., Seals D.F., Pass I., Salinsky D., Maurer L., Roth T.M., RA Courtneidge S.A.; RT "The adaptor protein fish associates with members of the ADAMs family RT and localizes to podosomes of Src-transformed cells."; RL J. Biol. Chem. 278:16844-16851(2003). RN [6] RP FUNCTION, SUBCELLULAR LOCATION, DOMAIN, AND TISSUE SPECIFICITY. RX PubMed=15710328; DOI=10.1016/j.ccr.2005.01.006; RA Seals D.F., Azucena E.F. Jr., Pass I., Tesfay L., Gordon R., RA Woodrow M., Resau J.H., Courtneidge S.A.; RT "The adaptor protein Tks5/Fish is required for podosome formation and RT function, and for the protease-driven invasion of cancer cells."; RL Cancer Cell 7:155-165(2005). RN [7] RP FUNCTION, INTERACTION WITH ADAM12, AND PTM. RX PubMed=15710903; DOI=10.1073/pnas.0408237102; RA Malinin N.L., Wright S., Seubert P., Schenk D., Griswold-Prenner I.; RT "Amyloid-beta neurotoxicity is mediated by FISH adapter protein and RT ADAM12 metalloprotease activity."; RL Proc. Natl. Acad. Sci. U.S.A. 102:3058-3063(2005). RN [8] RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. RC TISSUE=Embryonic kidney; RX PubMed=17525332; DOI=10.1126/science.1140321; RA Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, RA Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N., RA Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J.; RT "ATM and ATR substrate analysis reveals extensive protein networks RT responsive to DNA damage."; RL Science 316:1160-1166(2007). RN [9] RP SUBCELLULAR LOCATION. RX PubMed=18417249; DOI=10.1016/j.ejcb.2008.02.008; RA Blouw B., Seals D.F., Pass I., Diaz B., Courtneidge S.A.; RT "A role for the podosome/invadopodia scaffold protein Tks5 in tumor RT growth in vivo."; RL Eur. J. Cell Biol. 87:555-567(2008). RN [10] RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-421; SER-547; SER-567; RP SER-593; SER-644; THR-731; SER-1002; SER-1016; SER-1017 AND SER-1038, RP AND MASS SPECTROMETRY. RC TISSUE=Cervix carcinoma; RX PubMed=18669648; DOI=10.1073/pnas.0805139105; RA Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., RA Elledge S.J., Gygi S.P.; RT "A quantitative atlas of mitotic phosphorylation."; RL Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008). RN [11] RP INTERACTION WITH FASLG. RX PubMed=19807924; DOI=10.1186/1471-2172-10-53; RA Voss M., Lettau M., Janssen O.; RT "Identification of SH3 domain interaction partners of human FasL RT (CD178) by phage display screening."; RL BMC Immunol. 10:53-53(2009). RN [12] RP FUNCTION, AND INTERACTION WITH NOXA1. RX PubMed=19755710; DOI=10.1126/scisignal.2000370; RA Gianni D., Diaz B., Taulet N., Fowler B., Courtneidge S.A., RA Bokoch G.M.; RT "Novel p47(phox)-related organizers regulate localized NADPH oxidase 1 RT (Nox1) activity."; RL Sci. Signal. 2:RA54-RA54(2009). RN [13] RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1002, AND MASS RP SPECTROMETRY. RC TISSUE=Cervix carcinoma; RX PubMed=20068231; DOI=10.1126/scisignal.2000475; RA Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., RA Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., RA Mann M.; RT "Quantitative phosphoproteomics reveals widespread full RT phosphorylation site occupancy during mitosis."; RL Sci. Signal. 3:RA3-RA3(2010). RN [14] RP FUNCTION, AND INTERACTION WITH NOXA1 AND NOXO1. RX PubMed=20609497; DOI=10.1016/j.ejcb.2010.05.007; RA Gianni D., Dermardirossian C., Bokoch G.M.; RT "Direct interaction between Tks proteins and the N-terminal proline- RT rich region (PRR) of NoxA1 mediates Nox1-dependent ROS generation."; RL Eur. J. Cell Biol. 90:164-171(2011). RN [15] RP STRUCTURE BY NMR OF 168-325 AND 1072-1133. RG RIKEN structural genomics initiative (RSGI); RT "Solution structure of the SH3 domains from human KIAA0418 protein."; RL Submitted (AUG-2007) to the PDB data bank. CC -!- FUNCTION: Adapter protein involved in invadopodia and podosome CC formation, extracellular matrix degradation and invasiveness of CC some cancer cells. Binds matrix metalloproteinases (ADAMs), NADPH CC oxidases (NOXs) and phosphoinositides. Acts as an organizer CC protein that allows NOX1- or NOX3-dependent reactive oxygen CC species (ROS) generation and ROS localization. In association with CC ADAM12, mediates the neurotoxic effect of beta-amyloid peptide. CC -!- SUBUNIT: Interacts (via N-terminus) with CYBA (By similarity). CC Interacts with ADAM12, ADAM15 and ADAM19. Interacts with NOXO1. CC Interacts (via SH3 domains) with NOXA1. Interacts with FASLG. CC -!- INTERACTION: CC Q13444:ADAM15; NbExp=2; IntAct=EBI-2483234, EBI-77818; CC P21575:Dnm1 (xeno); NbExp=2; IntAct=EBI-7014859, EBI-80070; CC Q07889:SOS1; NbExp=5; IntAct=EBI-7014859, EBI-297487; CC -!- SUBCELLULAR LOCATION: Cytoplasm. Cell projection, podosome. CC Note=Cytoplasmic in normal cells and localizes to podosomes in CC SRC-transformed cells. CC -!- ALTERNATIVE PRODUCTS: CC Event=Alternative splicing; Named isoforms=3; CC Name=1; CC IsoId=Q5TCZ1-1; Sequence=Displayed; CC Name=2; CC IsoId=Q5TCZ1-2; Sequence=VSP_023312, VSP_023313; CC Name=3; CC IsoId=Q5TCZ1-3; Sequence=VSP_023313; CC Note=Gene prediction based on similarity to mouse ortholog and CC partial transcript data; CC -!- TISSUE SPECIFICITY: Found in several cancer cell lines, CC particularly invasive breast carcinomas and melanomas. CC -!- DOMAIN: The PX domain is required for podosome localization CC because of its ability to bind phosphatidylinositol 3-phosphate CC (PtdIns(3)P) and phosphatidylinositol 3,4-bisphosphate CC (PtdIns(3,4)P2) and, to a lesser extent, phosphatidylinositol 4- CC phosphate (PtdIns(4)P), phosphatidylinositol 5-phosphate CC (PtdIns(5)P), and phosphatidylinositol 3,5-bisphosphate CC (PtdIns(3,5)P2). Binds to the third intramolecular SH3 domain (By CC similarity). CC -!- DOMAIN: The fifth SH3 domain mediates binding with ADAM12, ADAM15 CC and ADAM19. CC -!- PTM: Tyrosine phosphorylated by SRC. Phosphorylation plays a CC regulatory role in the protein localization. The intramolecular CC interaction of the PX domain with the third SH3 domain maintains CC the protein in the cytoplasm and phosphorylation disrupts this CC interaction, resulting in the redistribution of the protein from CC cytoplasm to the perimembrane region. Phosphorylated on serine CC upon DNA damage, probably by ATM or ATR. CC -!- SIMILARITY: Belongs to the SH3PXD2 family. CC -!- SIMILARITY: Contains 1 PX (phox homology) domain. CC -!- SIMILARITY: Contains 5 SH3 domains. CC -!- SEQUENCE CAUTION: CC Sequence=BAA24848.2; Type=Erroneous initiation; Note=Translation N-terminally shortened; CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AB007878; BAA24848.2; ALT_INIT; mRNA. DR EMBL; AL121929; CAI13961.1; -; Genomic_DNA. DR EMBL; AL133355; CAI13961.1; JOINED; Genomic_DNA. DR EMBL; AL121929; CAI13962.1; -; Genomic_DNA. DR EMBL; AL133355; CAI13962.1; JOINED; Genomic_DNA. DR EMBL; AL121929; CAI13963.1; -; Genomic_DNA. DR EMBL; AL133355; CAI15351.1; -; Genomic_DNA. DR EMBL; AL121929; CAI15351.1; JOINED; Genomic_DNA. DR EMBL; AL133355; CAI15352.1; -; Genomic_DNA. DR EMBL; AL121929; CAI15352.1; JOINED; Genomic_DNA. DR EMBL; CH471066; EAW49623.1; -; Genomic_DNA. DR EMBL; CH471066; EAW49624.1; -; Genomic_DNA. DR PIR; T00056; T00056. DR RefSeq; NP_055446.2; NM_014631.2. DR RefSeq; XP_005270351.1; XM_005270294.1. DR UniGene; Hs.678727; -. DR PDB; 2DNU; NMR; -; A=268-325. DR PDB; 2EGA; NMR; -; A=168-224. DR PDB; 2EGC; NMR; -; A=1072-1133. DR PDB; 2EKH; NMR; -; A=842-908. DR PDBsum; 2DNU; -. DR PDBsum; 2EGA; -. DR PDBsum; 2EGC; -. DR PDBsum; 2EKH; -. DR ProteinModelPortal; Q5TCZ1; -. DR SMR; Q5TCZ1; 6-126, 162-329, 448-506, 842-908, 1072-1133. DR BioGrid; 115002; 11. DR IntAct; Q5TCZ1; 12. DR MINT; MINT-2792114; -. DR STRING; 9606.ENSP00000348215; -. DR PhosphoSite; Q5TCZ1; -. DR DMDM; 74746151; -. DR PaxDb; Q5TCZ1; -. DR PRIDE; Q5TCZ1; -. DR DNASU; 9644; -. DR Ensembl; ENST00000355946; ENSP00000348215; ENSG00000107957. DR Ensembl; ENST00000369774; ENSP00000358789; ENSG00000107957. DR GeneID; 9644; -. DR KEGG; hsa:9644; -. DR UCSC; uc010qqu.1; human. DR CTD; 9644; -. DR GeneCards; GC10M105345; -. DR HGNC; HGNC:23664; SH3PXD2A. DR HPA; HPA037922; -. DR HPA; HPA037923; -. DR neXtProt; NX_Q5TCZ1; -. DR PharmGKB; PA134956816; -. DR eggNOG; NOG148927; -. DR HOGENOM; HOG000154376; -. DR HOVERGEN; HBG089589; -. DR InParanoid; Q5TCZ1; -. DR OMA; LESGWWY; -. DR OrthoDB; EOG7H4DSQ; -. DR TreeFam; TF329347; -. DR ChiTaRS; SH3PXD2A; human. DR EvolutionaryTrace; Q5TCZ1; -. DR GeneWiki; SH3PXD2A; -. DR GenomeRNAi; 9644; -. DR NextBio; 36199; -. DR PRO; PR:Q5TCZ1; -. DR ArrayExpress; Q5TCZ1; -. DR Bgee; Q5TCZ1; -. DR CleanEx; HS_SH3PXD2A; -. DR Genevestigator; Q5TCZ1; -. DR GO; GO:0030054; C:cell junction; IEA:UniProtKB-KW. DR GO; GO:0042995; C:cell projection; IEA:UniProtKB-KW. DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell. DR GO; GO:0002102; C:podosome; IDA:UniProtKB. DR GO; GO:0035091; F:phosphatidylinositol binding; IEA:InterPro. DR GO; GO:0007154; P:cell communication; IEA:InterPro. DR GO; GO:0006801; P:superoxide metabolic process; IDA:UniProtKB. DR Gene3D; 3.30.1520.10; -; 1. DR InterPro; IPR001683; Phox. DR InterPro; IPR001452; SH3_domain. DR Pfam; PF00787; PX; 1. DR Pfam; PF00018; SH3_1; 4. DR SMART; SM00312; PX; 1. DR SMART; SM00326; SH3; 5. DR SUPFAM; SSF50044; SSF50044; 5. DR SUPFAM; SSF64268; SSF64268; 1. DR PROSITE; PS50195; PX; 1. DR PROSITE; PS50002; SH3; 5. PE 1: Evidence at protein level; KW 3D-structure; Alternative splicing; Cell junction; Cell projection; KW Coiled coil; Complete proteome; Cytoplasm; Phosphoprotein; KW Polymorphism; Reference proteome; Repeat; SH3 domain. FT CHAIN 1 1133 SH3 and PX domain-containing protein 2A. FT /FTId=PRO_0000278488. FT DOMAIN 4 128 PX. FT DOMAIN 166 225 SH3 1. FT DOMAIN 266 325 SH3 2. FT DOMAIN 448 507 SH3 3. FT DOMAIN 840 899 SH3 4. FT DOMAIN 1071 1133 SH3 5. FT COILED 917 946 Potential. FT COMPBIAS 634 724 Ser-rich. FT MOD_RES 421 421 Phosphoserine. FT MOD_RES 547 547 Phosphoserine. FT MOD_RES 567 567 Phosphoserine. FT MOD_RES 593 593 Phosphoserine. FT MOD_RES 644 644 Phosphoserine. FT MOD_RES 731 731 Phosphothreonine. FT MOD_RES 819 819 Phosphoserine (By similarity). FT MOD_RES 1002 1002 Phosphoserine. FT MOD_RES 1016 1016 Phosphoserine. FT MOD_RES 1017 1017 Phosphoserine. FT MOD_RES 1038 1038 Phosphoserine. FT VAR_SEQ 1 165 Missing (in isoform 2). FT /FTId=VSP_023312. FT VAR_SEQ 240 267 Missing (in isoform 2 and isoform 3). FT /FTId=VSP_023313. FT VARIANT 659 659 K -> Q (in dbSNP:rs11818820). FT /FTId=VAR_030781. FT VARIANT 1035 1035 R -> Q (in dbSNP:rs3781365). FT /FTId=VAR_030782. FT VARIANT 1073 1073 L -> P (in dbSNP:rs12764700). FT /FTId=VAR_056993. FT MUTAGEN 42 42 R->A: Loss of binding to (PtdIns(3)P) and FT (PtdIns(3,4)P2). FT MUTAGEN 93 93 R->A: Loss of binding to (PtdIns(3)P) and FT (PtdIns(3,4)P2). FT STRAND 181 184 FT STRAND 194 198 FT STRAND 202 207 FT STRAND 212 216 FT HELIX 217 219 FT STRAND 221 223 FT STRAND 268 272 FT STRAND 292 295 FT STRAND 300 308 FT STRAND 311 316 FT HELIX 317 319 FT STRAND 843 847 FT STRAND 866 872 FT STRAND 876 882 FT STRAND 885 890 FT TURN 891 893 FT STRAND 1075 1079 FT STRAND 1087 1089 FT STRAND 1097 1100 FT STRAND 1107 1113 FT STRAND 1116 1118 FT STRAND 1120 1125 FT HELIX 1126 1128 FT STRAND 1129 1131 SQ SEQUENCE 1133 AA; 125289 MW; D485F49E9192359C CRC64; MLAYCVQDAT VVDVEKRRNP SKHYVYIINV TWSDSTSQTI YRRYSKFFDL QMQLLDKFPI EGGQKDPKQR IIPFLPGKIL FRRSHIRDVA VKRLKPIDEY CRALVRLPPH ISQCDEVFRF FEARPEDVNP PKEDYGSSKR KSVWLSSWAE SPKKDVTGAD ATAEPMILEQ YVVVSNYKKQ ENSELSLQAG EVVDVIEKNE SGWWFVSTSE EQGWVPATYL EAQNGTRDDS DINTSKTGEV SKRRKAHLRR LDRRWTLGGM VNRQHSREEK YVTVQPYTSQ SKDEIGFEKG VTVEVIRKNL EGWWYIRYLG KEGWAPASYL KKAKDDLPTR KKNLAGPVEI IGNIMEISNL LNKKASGDKE TPPAEGEGHE APIAKKEISL PILCNASNGS AVGVPDRTVS RLAQGSPAVA RIAPQRAQIS SPNLRTRPPP RRESSLGFQL PKPPEPPSVE VEYYTIAEFQ SCISDGISFR GGQKAEVIDK NSGGWWYVQI GEKEGWAPAS YIDKRKKPNL SRRTSTLTRP KVPPPAPPSK PKEAEEGPTG ASESQDSPRK LKYEEPEYDI PAFGFDSEPE LSEEPVEDRA SGERRPAQPH RPSPASSLQR ARFKVGESSE DVALEEETIY ENEGFRPYAE DTLSARGSSG DSDSPGSSSL SLTRKNSPKS GSPKSSSLLK LKAEKNAQAE MGKNHSSASF SSSITINTTC CSSSSSSSSS LSKTSGDLKP RSASDAGIRG TPKVRAKKDA DANAGLTSCP RAKPSVRPKP FLNRAESQSQ EKMDISTLRR QLRPTGQLRG GLKGSKSEDS ELPPQTASEA PSEGSRRSSS DLITLPATTP PCPTKKEWEG PATSYMTCSA YQKVQDSEIS FPAGVEVQVL EKQESGWWYV RFGELEGWAP SHYLVLDENE QPDPSGKELD TVPAKGRQNE GKSDSLEKIE RRVQALNTVN QSKKATPPIP SKPPGGFGKT SGTPAVKMRN GVRQVAVRPQ SVFVSPPPKD NNLSCALRRN ESLTATDGLR GVRRNSSFST ARSAAAEAKG RLAERAASQG SDSPLLPAQR NSIPVSPVRP KPIEKSQFIH NNLKDVYVSI ADYEGDEETA GFQEGVSMEV LERNPNGWWY CQILDGVKPF KGWVPSNYLE KKN //