ID Q5JET1_PYRKO PRELIMINARY; PRT; 735 AA. AC Q5JET1; DT 10-MAY-2005 (TrEMBLrel. 30, Created) DT 10-MAY-2005 (TrEMBLrel. 30, Last sequence update) DT 10-MAY-2005 (TrEMBLrel. 30, Last annotation update) DE DNA polymerase II, small subunit. GN OrderedLocusNames=TK1902; OS Pyrococcus kodakaraensis (Thermococcus kodakaraensis). OC Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae; OC Thermococcus. OX NCBI_TaxID=69014; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=KOD1; RX PubMed=15710748; DOI=10.1101/gr.3003105; RA Fukui T., Atomi H., Kanai T., Matsumi R., Fujiwara S., Imanaka T.; RT "Complete genome sequence of the hyperthermophilic archaeon RT Thermococcus kodakaraensis KOD1 and comparison with Pyrococcus RT genomes."; RL Genome Res. 15:352-363(2005). CC -!- FUNCTION: Possesses two activities: a DNA synthesis (polymerase) CC and an exonucleolytic activity that degrades single stranded DNA CC in the 3' to 5' direction. Has a template-primer preference which CC is characteristic of a replicative DNA polymerase (By similarity). CC -!- CATALYTIC ACTIVITY: Degradation of single-stranded DNA. It acts CC progressively in a 3'- to 5'-direction, releasing nucleoside 5'- CC phosphates. CC -!- CATALYTIC ACTIVITY: Deoxynucleoside triphosphate + DNA(n) = CC diphosphate + DNA(n+1). CC -!- SUBUNIT: Heterodimer of a large subunit and a small subunit (By CC similarity). CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AP006878; BAD86091.1; -; Genomic_DNA. DR GO; GO:0005634; C:nucleus; IEA. DR GO; GO:0008408; F:3'-5' exonuclease activity; IEA. DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA. DR GO; GO:0016787; F:hydrolase activity; IEA. DR GO; GO:0003676; F:nucleic acid binding; IEA. DR GO; GO:0006260; P:DNA replication; IEA. DR InterPro; IPR011149; Arc_Pol2_small. DR InterPro; IPR004843; M-pesterase. DR InterPro; IPR004365; OB-fold_tRNA. DR Pfam; PF00149; Metallophos; 1. DR Pfam; PF01336; tRNA_anti; 1. DR PIRSF; PIRSF000803; Arc_Pol2_small; 1. KW Complete proteome; DNA replication; DNA-binding; KW DNA-directed DNA polymerase; Exonuclease; Hydrolase; KW Multifunctional enzyme; Nuclease; Nucleotidyltransferase; Transferase. SQ SEQUENCE 735 AA; 80783 MW; A84B94CBC2614C3B CRC64; MLVEDLLKNN YLITPSAYYL LSDHYKKAFT LAELIKFAKN RGTFVVDSNL AREFLAEKGI ISSGNGALDG SVLSGISVSE SPVKPNGEAV LESESDESGA IESSLQEGVE DGVESIAEPS AASVEDVVVA GESSLASEGS SLEDEINTEP ELSSSMDDGA EVVQSSEGDE TSISTGNALG SVDYGGSDSE SEALEEASSG GESFISTGTP EDEALLEESP EGEFPDENGF SDESLPVENG SENGYGDSEE YYENGDNGVK PKIVYGDYGV PIAYVADETP EEEKAYSTYS DLVIAPKEGF HYRAKEIPDE WELAFDVKNV KFEVPKVKNA QSKEGEVIIQ AYSSYFKSRL KKMRRIFREN PEIGTIVDIA KLSYVREDDV TIIGLVNEKR ETRKGYLFEI EDATGRIKVF IGSDKEGANE AYSTIMPDSV VAFRGTPGKG IFFANRVFLP DVPKFKRSKP PLEEKVYAIL LSDIHVGSNK FCEEAFIKFL EWLNGEVNSR TEEELVSRIK YIIIGGDVVD GVGIYPGQYN ELAIPDIFDQ YEALANLLKQ VPDHITMFIG PGNHDAARTA LPQPGFYEEY AKPLYKLKNA VIISNPAVIR LHGRDFLVAH GRGIEDVVDF VPNRSHHRPA EAMVELLKLR HIAPTFGNKV PIAPDPEDTL VIESVPDLFQ AGHVHVMQYK TYNGVFVINT GTWQAQTEFQ KMVNIIPTPA RVPIIDVETA RLRAVVRFDQ FCEGV //