ID Q5FI02_LACAC Unreviewed; 551 AA. AC Q5FI02; DT 01-MAR-2005, integrated into UniProtKB/TrEMBL. DT 01-MAR-2005, sequence version 1. DT 27-NOV-2024, entry version 92. DE SubName: Full=Oligo-1,6-glucosidase {ECO:0000313|EMBL:AAV43672.1}; DE EC=3.2.1.10 {ECO:0000313|EMBL:AAV43672.1}; GN OrderedLocusNames=LBA1872 {ECO:0000313|EMBL:AAV43672.1}; OS Lactobacillus acidophilus (strain ATCC 700396 / NCK56 / N2 / NCFM). OC Bacteria; Bacillota; Bacilli; Lactobacillales; Lactobacillaceae; OC Lactobacillus. OX NCBI_TaxID=272621 {ECO:0000313|Proteomes:UP000006381}; RN [1] {ECO:0000313|EMBL:AAV43672.1, ECO:0000313|Proteomes:UP000006381} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=ATCC 700396 / NCK56 / N2 / NCFM RC {ECO:0000313|Proteomes:UP000006381}; RX PubMed=15671160; DOI=10.1073/pnas.0409188102; RA Altermann E., Russell W.M., Azcarate-Peril M.A., Barrangou R., Buck B.L., RA McAuliffe O., Souther N., Dobson A., Duong T., Callanan M., Lick S., RA Hamrick A., Cano R., Klaenhammer T.R.; RT "Complete genome sequence of the probiotic lactic acid bacterium RT Lactobacillus acidophilus NCFM."; RL Proc. Natl. Acad. Sci. U.S.A. 102:3906-3912(2005). RN [2] {ECO:0007829|PDB:6Y9T} RP X-RAY CRYSTALLOGRAPHY (2.78 ANGSTROMS) IN COMPLEX WITH CA(2+). RX PubMed=32444471; DOI=10.1128/AEM.00661-20; RA Andersen S., Moller M.S., Poulsen J.N., Pichler M.J., Svensson B., RA Lo Leggio L., Goh Y.J., Abou Hachem M.; RT "An 1,4-alpha-Glucosyltransferase Defines a New Maltodextrin Catabolism RT Scheme in Lactobacillus acidophilus. ."; RL Appl. Environ. Microbiol. 86:e00661-e00620(2020). CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; CP000033; AAV43672.1; -; Genomic_DNA. DR RefSeq; WP_011254601.1; NC_006814.3. DR RefSeq; YP_194703.1; NC_006814.3. DR PDB; 6Y9T; X-ray; 2.78 A; A/B=1-551. DR AlphaFoldDB; Q5FI02; -. DR SMR; Q5FI02; -. DR STRING; 272621.LBA1872; -. DR CAZy; GH13; Glycoside Hydrolase Family 13. DR GeneID; 56943420; -. DR KEGG; lac:LBA1872; -. DR PATRIC; fig|272621.13.peg.1779; -. DR eggNOG; COG0366; Bacteria. DR HOGENOM; CLU_006462_2_3_9; -. DR OrthoDB; 9805159at2; -. DR BioCyc; LACI272621:G1G49-1826-MONOMER; -. DR Proteomes; UP000006381; Chromosome. DR GO; GO:0004556; F:alpha-amylase activity; IEA:TreeGrafter. DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW. DR GO; GO:0004574; F:oligo-1,6-glucosidase activity; IEA:UniProtKB-EC. DR GO; GO:0009313; P:oligosaccharide catabolic process; IEA:TreeGrafter. DR CDD; cd11333; AmyAc_SI_OligoGlu_DGase; 1. DR FunFam; 3.90.400.10:FF:000002; Sucrose isomerase; 1. DR Gene3D; 3.20.20.80; Glycosidases; 1. DR Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1. DR InterPro; IPR006047; Glyco_hydro_13_cat_dom. DR InterPro; IPR013780; Glyco_hydro_b. DR InterPro; IPR017853; Glycoside_hydrolase_SF. DR InterPro; IPR045857; O16G_dom_2. DR PANTHER; PTHR10357; ALPHA-AMYLASE FAMILY MEMBER; 1. DR PANTHER; PTHR10357:SF179; NEUTRAL AND BASIC AMINO ACID TRANSPORT PROTEIN RBAT; 1. DR Pfam; PF00128; Alpha-amylase; 1. DR SMART; SM00642; Aamy; 1. DR SUPFAM; SSF51445; (Trans)glycosidases; 1. DR SUPFAM; SSF51011; Glycosyl hydrolase domain; 1. PE 1: Evidence at protein level; KW 3D-structure {ECO:0007829|PDB:6Y9T}; Calcium {ECO:0007829|PDB:6Y9T}; KW Glycosidase {ECO:0000313|EMBL:AAV43672.1}; KW Hydrolase {ECO:0000313|EMBL:AAV43672.1}; KW Metal-binding {ECO:0007829|PDB:6Y9T}; KW Reference proteome {ECO:0000313|Proteomes:UP000006381}. FT DOMAIN 12..403 FT /note="Glycosyl hydrolase family 13 catalytic" FT /evidence="ECO:0000259|SMART:SM00642" FT BINDING 20 FT /ligand="Ca(2+)" FT /ligand_id="ChEBI:CHEBI:29108" FT /evidence="ECO:0007829|PDB:6Y9T" FT BINDING 22 FT /ligand="Ca(2+)" FT /ligand_id="ChEBI:CHEBI:29108" FT /evidence="ECO:0007829|PDB:6Y9T" FT BINDING 24 FT /ligand="Ca(2+)" FT /ligand_id="ChEBI:CHEBI:29108" FT /evidence="ECO:0007829|PDB:6Y9T" FT BINDING 26 FT /ligand="Ca(2+)" FT /ligand_id="ChEBI:CHEBI:29108" FT /evidence="ECO:0007829|PDB:6Y9T" FT BINDING 28 FT /ligand="Ca(2+)" FT /ligand_id="ChEBI:CHEBI:29108" FT /evidence="ECO:0007829|PDB:6Y9T" SQ SEQUENCE 551 AA; 63163 MW; 250AF435FD61D4D9 CRC64; MSHWYDHAII YQIYPKSFQD SNDDGIGDLN GIRKRIPYLQ NLGVNAVWLN PVFVSPQVDN GYDVSNYFAI DSHMGTMEDM ENLIKDLHKA GIHIIMDFVL NHTSDQHPWF QDAIKNPDSL YRDYYIFAGH DNKQPNNWGS FFGGSVWEPD PAGTGQSYFH LFDKRMPDLN WKNPEVRHAM LEIAEFWLKK GIDGLRLDAF IHIGKADLRQ NYPAMDDKPV IAEPFFANLP QVQEWMRPFC EQIKEDYPDA LLLGEAASAS VNLAVDYTNK RNHLMDCVIT FRYFTEDDSK IDKSYSAQYQ PKELDLTAFK QNQVVWQQTL ADISQPTLYW NNHDMARLAT RIAKTSTQAK SLAMLMYLQR GIPIIYYGEE LGLKNLHFTS VDQFEDQTVA PWIKEAQKAG ISRDAAFAMV SDTHKLPARG PMPWNDTENN GFTSAKPWLN GISQDDVTVA NEVNSDNSMF TFYKNMLNLK KEKLFQDGTY YMISTGKDSY VYQRDLGNES AIVAVSLSNK KISIDLPEEY IKELLKAGEY QLTNGKLTLM PYAGVVLKKE N //