ID   Q5E0J2_ALIF1            Unreviewed;       634 AA.
AC   Q5E0J2;
DT   15-MAR-2005, integrated into UniProtKB/TrEMBL.
DT   15-MAR-2005, sequence version 1.
DT   19-JAN-2022, entry version 110.
DE   RecName: Full=ATP-dependent RNA helicase DeaD {ECO:0000256|HAMAP-Rule:MF_00964};
DE            EC=3.6.4.13 {ECO:0000256|HAMAP-Rule:MF_00964};
DE   AltName: Full=Cold-shock DEAD box protein A {ECO:0000256|HAMAP-Rule:MF_00964};
GN   Name=deaD {ECO:0000256|HAMAP-Rule:MF_00964,
GN   ECO:0000313|EMBL:AAW87454.1};
GN   Synonyms=csdA {ECO:0000256|HAMAP-Rule:MF_00964};
GN   OrderedLocusNames=VF_A0384 {ECO:0000313|EMBL:AAW87454.1};
OS   Aliivibrio fischeri (strain ATCC 700601 / ES114) (Vibrio fischeri).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC   Aliivibrio.
OX   NCBI_TaxID=312309 {ECO:0000313|EMBL:AAW87454.1, ECO:0000313|Proteomes:UP000000537};
RN   [1] {ECO:0000313|EMBL:AAW87454.1, ECO:0000313|Proteomes:UP000000537}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700601 / ES114 {ECO:0000313|Proteomes:UP000000537};
RX   PubMed=15703294; DOI=10.1073/pnas.0409900102;
RA   Ruby E.G., Urbanowski M., Campbell J., Dunn A., Faini M., Gunsalus R.,
RA   Lostroh P., Lupp C., McCann J., Millikan D., Schaefer A., Stabb E.,
RA   Stevens A., Visick K., Whistler C., Greenberg E.P.;
RT   "Complete genome sequence of Vibrio fischeri: a symbiotic bacterium with
RT   pathogenic congeners.";
RL   Proc. Natl. Acad. Sci. U.S.A. 102:3004-3009(2005).
CC   -!- FUNCTION: DEAD-box RNA helicase involved in various cellular processes
CC       at low temperature, including ribosome biogenesis, mRNA degradation and
CC       translation initiation. {ECO:0000256|HAMAP-Rule:MF_00964}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC         Evidence={ECO:0000256|HAMAP-Rule:MF_00964};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00964}.
CC   -!- SIMILARITY: Belongs to the DEAD box helicase family. DeaD/CsdA
CC       subfamily. {ECO:0000256|HAMAP-Rule:MF_00964}.
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DR   EMBL; CP000021; AAW87454.1; -; Genomic_DNA.
DR   RefSeq; WP_011263263.1; NC_006841.2.
DR   RefSeq; YP_206342.1; NC_006841.2.
DR   STRING; 312309.VF_A0384; -.
DR   EnsemblBacteria; AAW87454; AAW87454; VF_A0384.
DR   KEGG; vfi:VF_A0384; -.
DR   PATRIC; fig|312309.11.peg.2987; -.
DR   eggNOG; COG0513; Bacteria.
DR   HOGENOM; CLU_003041_21_1_6; -.
DR   OMA; LPQGMPK; -.
DR   OrthoDB; 626183at2; -.
DR   Proteomes; UP000000537; Chromosome II.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR   GO; GO:0008094; F:ATP-dependent activity, acting on DNA; IEA:UniProt.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0070417; P:cellular response to cold; IEA:InterPro.
DR   GO; GO:0000027; P:ribosomal large subunit assembly; IEA:UniProtKB-UniRule.
DR   GO; GO:0006401; P:RNA catabolic process; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.30.70.330; -; 1.
DR   Gene3D; 3.40.50.300; -; 2.
DR   HAMAP; MF_00964; DEAD_helicase_DeaD; 1.
DR   InterPro; IPR005580; DbpA/CsdA_RNA-bd_dom.
DR   InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR   InterPro; IPR028618; DEAD_helicase_DeaD.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR001650; Helicase_C.
DR   InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR000629; RNA-helicase_DEAD-box_CS.
DR   InterPro; IPR014014; RNA_helicase_DEAD_Q_motif.
DR   Pfam; PF03880; DbpA; 1.
DR   Pfam; PF00270; DEAD; 1.
DR   Pfam; PF00271; Helicase_C; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM00490; HELICc; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS00039; DEAD_ATP_HELICASE; 1.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
DR   PROSITE; PS51195; Q_MOTIF; 1.
PE   3: Inferred from homology;
KW   ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|HAMAP-
KW   Rule:MF_00964}; Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00964};
KW   Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|HAMAP-Rule:MF_00964};
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|HAMAP-Rule:MF_00964};
KW   Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|HAMAP-
KW   Rule:MF_00964}; Reference proteome {ECO:0000313|Proteomes:UP000000537};
KW   RNA-binding {ECO:0000256|HAMAP-Rule:MF_00964};
KW   Stress response {ECO:0000256|HAMAP-Rule:MF_00964}.
FT   DOMAIN          6..34
FT                   /note="Q_MOTIF"
FT                   /evidence="ECO:0000259|PROSITE:PS51195"
FT   DOMAIN          37..208
FT                   /note="Helicase ATP-binding"
FT                   /evidence="ECO:0000259|PROSITE:PS51192"
FT   DOMAIN          231..379
FT                   /note="Helicase C-terminal"
FT                   /evidence="ECO:0000259|PROSITE:PS51194"
FT   REGION          449..483
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          566..634
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           6..34
FT                   /note="Q motif"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU00552"
FT   COMPBIAS        587..634
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   634 AA;  70373 MW;  1F8F4153261FC71D CRC64;
     MSESITEFRQ LALDESILSA LDDMGFVAPT PIQAASIPLL LEGRDALGKA QTGTGKTAAF
     SLPLLNKINL KQHNPQAIIM APTRELAIQV AAEVKNLGRN IKGLKVLEIY GGASIVDQMR
     ALSRGAHIIV GTPGRVKDLL NRDRLNLGEV HTFILDEADE MLKMGFVDDV TWILEKAPDT
     AQRILFSATM PPMVKTIVDR YLREPARVDV AGTNHTVDKV EQNFWVVKGV EKDEAMSRLL
     ETEETDASIV FVRTRQDTER LADWLSARGF KAAALHGDIP QSQRERTVDN IKNGVIDILV
     ATDVVARGLD VPRITHVFNY DIPFDVESYI HRIGRTGRAG RKGKAILLVR TNQMRMLRTI
     ERVTKSTMEE IQLPHRDQVA ECRLAALAAE LQADKEFVAL DAFTDLINKL QETLEIDATT
     IAAMLLKRQQ GKRPLFYKGP DPMIAAMERA KNRRSDRGER GERGGRGDRP ERGERRQYNN
     DDFDTYQLEV GREQGVQVKD IVGALANELG FTKGSIGAIK LAPGHTYVQL PKKMSADVAG
     KLKKLRIRQN ETKAVVVEGV DLTIERRPRT GGGRDDNRGG NGRGGYRGNR ENREGGRGGE
     RRFDRNKGGD HRGSHRGERA PGGRGRGRSE RSEG
//