ID Q5E0J2_ALIF1 Unreviewed; 634 AA. AC Q5E0J2; DT 15-MAR-2005, integrated into UniProtKB/TrEMBL. DT 15-MAR-2005, sequence version 1. DT 10-FEB-2021, entry version 106. DE RecName: Full=ATP-dependent RNA helicase DeaD {ECO:0000256|HAMAP-Rule:MF_00964}; DE EC=3.6.4.13 {ECO:0000256|HAMAP-Rule:MF_00964}; DE AltName: Full=Cold-shock DEAD box protein A {ECO:0000256|HAMAP-Rule:MF_00964}; GN Name=deaD {ECO:0000256|HAMAP-Rule:MF_00964, GN ECO:0000313|EMBL:AAW87454.1}; GN Synonyms=csdA {ECO:0000256|HAMAP-Rule:MF_00964}; GN OrderedLocusNames=VF_A0384 {ECO:0000313|EMBL:AAW87454.1}; OS Aliivibrio fischeri (strain ATCC 700601 / ES114) (Vibrio fischeri). OC Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae; OC Aliivibrio. OX NCBI_TaxID=312309 {ECO:0000313|EMBL:AAW87454.1, ECO:0000313|Proteomes:UP000000537}; RN [1] {ECO:0000313|EMBL:AAW87454.1, ECO:0000313|Proteomes:UP000000537} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=ATCC 700601 / ES114 {ECO:0000313|Proteomes:UP000000537}; RX PubMed=15703294; DOI=10.1073/pnas.0409900102; RA Ruby E.G., Urbanowski M., Campbell J., Dunn A., Faini M., Gunsalus R., RA Lostroh P., Lupp C., McCann J., Millikan D., Schaefer A., Stabb E., RA Stevens A., Visick K., Whistler C., Greenberg E.P.; RT "Complete genome sequence of Vibrio fischeri: a symbiotic bacterium with RT pathogenic congeners."; RL Proc. Natl. Acad. Sci. U.S.A. 102:3004-3009(2005). CC -!- FUNCTION: DEAD-box RNA helicase involved in various cellular processes CC at low temperature, including ribosome biogenesis, mRNA degradation and CC translation initiation. {ECO:0000256|HAMAP-Rule:MF_00964}. CC -!- CATALYTIC ACTIVITY: CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065, CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13; CC Evidence={ECO:0000256|HAMAP-Rule:MF_00964}; CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00964}. CC -!- SIMILARITY: Belongs to the DEAD box helicase family. DeaD/CsdA CC subfamily. {ECO:0000256|HAMAP-Rule:MF_00964}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; CP000021; AAW87454.1; -; Genomic_DNA. DR RefSeq; WP_011263263.1; NC_006841.2. DR RefSeq; YP_206342.1; NC_006841.2. DR STRING; 312309.VF_A0384; -. DR EnsemblBacteria; AAW87454; AAW87454; VF_A0384. DR KEGG; vfi:VF_A0384; -. DR PATRIC; fig|312309.11.peg.2987; -. DR eggNOG; COG0513; Bacteria. DR HOGENOM; CLU_003041_21_1_6; -. DR OMA; LPQGMPK; -. DR OrthoDB; 626183at2; -. DR Proteomes; UP000000537; Chromosome II. DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell. DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule. DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-UniRule. DR GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-UniRule. DR GO; GO:0070417; P:cellular response to cold; IEA:InterPro. DR GO; GO:0000027; P:ribosomal large subunit assembly; IEA:UniProtKB-UniRule. DR GO; GO:0006401; P:RNA catabolic process; IEA:UniProtKB-UniRule. DR Gene3D; 3.30.70.330; -; 1. DR HAMAP; MF_00964; DEAD_helicase_DeaD; 1. DR InterPro; IPR005580; DbpA/CsdA_RNA-bd_dom. DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom. DR InterPro; IPR028618; DEAD_helicase_DeaD. DR InterPro; IPR014001; Helicase_ATP-bd. DR InterPro; IPR001650; Helicase_C. DR InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf. DR InterPro; IPR027417; P-loop_NTPase. DR InterPro; IPR000629; RNA-helicase_DEAD-box_CS. DR InterPro; IPR014014; RNA_helicase_DEAD_Q_motif. DR Pfam; PF03880; DbpA; 1. DR Pfam; PF00270; DEAD; 1. DR Pfam; PF00271; Helicase_C; 1. DR SMART; SM00487; DEXDc; 1. DR SMART; SM00490; HELICc; 1. DR SUPFAM; SSF52540; SSF52540; 1. DR PROSITE; PS00039; DEAD_ATP_HELICASE; 1. DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1. DR PROSITE; PS51194; HELICASE_CTER; 1. DR PROSITE; PS51195; Q_MOTIF; 1. PE 3: Inferred from homology; KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|HAMAP- KW Rule:MF_00964, ECO:0000256|RuleBase:RU000492}; KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490, ECO:0000256|HAMAP-Rule:MF_00964}; KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|HAMAP-Rule:MF_00964, KW ECO:0000256|RuleBase:RU000492, ECO:0000313|EMBL:AAW87454.1}; KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|HAMAP-Rule:MF_00964, KW ECO:0000256|RuleBase:RU000492}; KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|HAMAP- KW Rule:MF_00964, ECO:0000256|RuleBase:RU000492}; KW Reference proteome {ECO:0000313|Proteomes:UP000000537}; KW RNA-binding {ECO:0000256|HAMAP-Rule:MF_00964}; KW Stress response {ECO:0000256|HAMAP-Rule:MF_00964}. FT DOMAIN 6..34 FT /note="Q_MOTIF" FT /evidence="ECO:0000259|PROSITE:PS51195" FT DOMAIN 37..208 FT /note="Helicase ATP-binding" FT /evidence="ECO:0000259|PROSITE:PS51192" FT DOMAIN 231..379 FT /note="Helicase C-terminal" FT /evidence="ECO:0000259|PROSITE:PS51194" FT REGION 449..483 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT REGION 566..634 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT MOTIF 6..34 FT /note="Q motif" FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00552" FT COMPBIAS 587..634 FT /note="Polyampholyte" FT /evidence="ECO:0000256|SAM:MobiDB-lite" SQ SEQUENCE 634 AA; 70373 MW; 1F8F4153261FC71D CRC64; MSESITEFRQ LALDESILSA LDDMGFVAPT PIQAASIPLL LEGRDALGKA QTGTGKTAAF SLPLLNKINL KQHNPQAIIM APTRELAIQV AAEVKNLGRN IKGLKVLEIY GGASIVDQMR ALSRGAHIIV GTPGRVKDLL NRDRLNLGEV HTFILDEADE MLKMGFVDDV TWILEKAPDT AQRILFSATM PPMVKTIVDR YLREPARVDV AGTNHTVDKV EQNFWVVKGV EKDEAMSRLL ETEETDASIV FVRTRQDTER LADWLSARGF KAAALHGDIP QSQRERTVDN IKNGVIDILV ATDVVARGLD VPRITHVFNY DIPFDVESYI HRIGRTGRAG RKGKAILLVR TNQMRMLRTI ERVTKSTMEE IQLPHRDQVA ECRLAALAAE LQADKEFVAL DAFTDLINKL QETLEIDATT IAAMLLKRQQ GKRPLFYKGP DPMIAAMERA KNRRSDRGER GERGGRGDRP ERGERRQYNN DDFDTYQLEV GREQGVQVKD IVGALANELG FTKGSIGAIK LAPGHTYVQL PKKMSADVAG KLKKLRIRQN ETKAVVVEGV DLTIERRPRT GGGRDDNRGG NGRGGYRGNR ENREGGRGGE RRFDRNKGGD HRGSHRGERA PGGRGRGRSE RSEG //