ID DBAF_EMENI Reviewed; 467 AA. AC Q5AUY0; A0A1U8QSV1; C8V4K0; DT 13-FEB-2019, integrated into UniProtKB/Swiss-Prot. DT 26-APR-2005, sequence version 1. DT 11-DEC-2019, entry version 104. DE RecName: Full=FAD-dependent oxidoreductase dbaF {ECO:0000303|PubMed:23001671}; DE EC=1.21.-.- {ECO:0000305|PubMed:23001671}; DE AltName: Full=Derivative of benzaldehyde biosynthesis cluster protein F {ECO:0000303|PubMed:23001671}; DE Flags: Precursor; GN Name=dbaF {ECO:0000303|PubMed:23001671}; ORFNames=ANIA_07900; OS Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / OS M139) (Aspergillus nidulans). OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes; OC Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus. OX NCBI_TaxID=227321; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139; RX PubMed=16372000; DOI=10.1038/nature04341; RA Galagan J.E., Calvo S.E., Cuomo C., Ma L.-J., Wortman J.R., Batzoglou S., RA Lee S.-I., Bastuerkmen M., Spevak C.C., Clutterbuck J., Kapitonov V., RA Jurka J., Scazzocchio C., Farman M.L., Butler J., Purcell S., Harris S., RA Braus G.H., Draht O., Busch S., D'Enfert C., Bouchier C., Goldman G.H., RA Bell-Pedersen D., Griffiths-Jones S., Doonan J.H., Yu J., Vienken K., RA Pain A., Freitag M., Selker E.U., Archer D.B., Penalva M.A., Oakley B.R., RA Momany M., Tanaka T., Kumagai T., Asai K., Machida M., Nierman W.C., RA Denning D.W., Caddick M.X., Hynes M., Paoletti M., Fischer R., Miller B.L., RA Dyer P.S., Sachs M.S., Osmani S.A., Birren B.W.; RT "Sequencing of Aspergillus nidulans and comparative analysis with A. RT fumigatus and A. oryzae."; RL Nature 438:1105-1115(2005). RN [2] RP GENOME REANNOTATION. RC STRAIN=FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139; RX PubMed=19146970; DOI=10.1016/j.fgb.2008.12.003; RA Wortman J.R., Gilsenan J.M., Joardar V., Deegan J., Clutterbuck J., RA Andersen M.R., Archer D., Bencina M., Braus G., Coutinho P., von Dohren H., RA Doonan J., Driessen A.J., Durek P., Espeso E., Fekete E., Flipphi M., RA Estrada C.G., Geysens S., Goldman G., de Groot P.W., Hansen K., RA Harris S.D., Heinekamp T., Helmstaedt K., Henrissat B., Hofmann G., RA Homan T., Horio T., Horiuchi H., James S., Jones M., Karaffa L., RA Karanyi Z., Kato M., Keller N., Kelly D.E., Kiel J.A., Kim J.M., RA van der Klei I.J., Klis F.M., Kovalchuk A., Krasevec N., Kubicek C.P., RA Liu B., Maccabe A., Meyer V., Mirabito P., Miskei M., Mos M., Mullins J., RA Nelson D.R., Nielsen J., Oakley B.R., Osmani S.A., Pakula T., Paszewski A., RA Paulsen I., Pilsyk S., Pocsi I., Punt P.J., Ram A.F., Ren Q., Robellet X., RA Robson G., Seiboth B., van Solingen P., Specht T., Sun J., RA Taheri-Talesh N., Takeshita N., Ussery D., vanKuyk P.A., Visser H., RA van de Vondervoort P.J., de Vries R.P., Walton J., Xiang X., Xiong Y., RA Zeng A.P., Brandt B.W., Cornell M.J., van den Hondel C.A., Visser J., RA Oliver S.G., Turner G.; RT "The 2008 update of the Aspergillus nidulans genome annotation: a community RT effort."; RL Fungal Genet. Biol. 46:S2-13(2009). RN [3] RP IDENTIFICATION, INDUCTION, FUNCTION, DISRUPTION PHENOTYPE, AND PATHWAY. RX PubMed=23001671; DOI=10.1128/aem.01808-12; RA Gerke J., Bayram O., Feussner K., Landesfeind M., Shelest E., Feussner I., RA Braus G.H.; RT "Breaking the silence: protein stabilization uncovers silenced biosynthetic RT gene clusters in the fungus Aspergillus nidulans."; RL Appl. Environ. Microbiol. 78:8234-8244(2012). RN [4] RP FUNCTION. RX PubMed=22510154; DOI=10.1021/ja3016395; RA Ahuja M., Chiang Y.M., Chang S.L., Praseuth M.B., Entwistle R., RA Sanchez J.F., Lo H.C., Yeh H.H., Oakley B.R., Wang C.C.; RT "Illuminating the diversity of aromatic polyketide synthases in Aspergillus RT nidulans."; RL J. Am. Chem. Soc. 134:8212-8221(2012). RN [5] RP INDUCTION. RX PubMed=25701285; DOI=10.1534/genetics.115.174342; RA Oiartzabal-Arano E., Garzia A., Gorostidi A., Ugalde U., Espeso E.A., RA Etxebeste O.; RT "Beyond asexual development: modifications in the gene expression profile RT caused by the absence of the Aspergillus nidulans transcription factor RT FlbB."; RL Genetics 199:1127-1142(2015). CC -!- FUNCTION: FAD-dependent oxidoreductase; part of the gene cluster that CC mediates the biosynthesis of the antibiotic 2,4-dihydroxy-3-methyl-6- CC (2-oxopropyl)benzaldehyde (DHMBA) and its derivatives (PubMed:22510154, CC PubMed:23001671). The direct non-reducing polyketide synthase dbaI CC product is 2,4-dihydroxy-3-methyl-6-(2-oxopropyl)benzaldehyde (DHMBA), CC produced by condensation of one acetyl-CoA starter unit with 4 malonyl- CC CoA units and one methylation step (PubMed:22510154). The FAD-dependent CC monooxygenase dbaH is responsible for the synthesis of yellow pigments CC derived from the oxidation of DHMBA (PubMed:23001671). The roles of CC dbaB, C, E and F have still to be determined (Probable). CC {ECO:0000269|PubMed:22510154, ECO:0000269|PubMed:23001671, CC ECO:0000305|PubMed:23001671}. CC -!- COFACTOR: CC Name=FAD; Xref=ChEBI:CHEBI:57692; CC Evidence={ECO:0000250|UniProtKB:B8NI10}; CC -!- PATHWAY: Secondary metabolite biosynthesis. CC {ECO:0000305|PubMed:23001671}. CC -!- INDUCTION: Deletion of the conserved eukaryotic csnE deneddylase CC subunit of the COP9 signalosome leading to defect in protein CC degradation results in the activation of the silenced dba gene cluster CC (PubMed:23001671). Expression is positively regulated by the dba CC cluster specific transcription factors dbaA and dbaG (PubMed:23001671). CC Expression is also controlled by the transcription factor flbB CC (PubMed:25701285). {ECO:0000269|PubMed:23001671, CC ECO:0000269|PubMed:25701285}. CC -!- DISRUPTION PHENOTYPE: Reduces the amounts of DHMDA produced. CC {ECO:0000269|PubMed:23001671}. CC -!- SIMILARITY: Belongs to the beta-cyclopiazonate dehydrogenase family. CC {ECO:0000305}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AACD01000135; EAA59554.1; -; Genomic_DNA. DR EMBL; BN001302; CBF73488.1; -; Genomic_DNA. DR RefSeq; XP_681169.1; XM_676077.1. DR EnsemblFungi; CBF73488; CBF73488; ANIA_07900. DR EnsemblFungi; EAA59554; EAA59554; AN7900.2. DR GeneID; 2869100; -. DR KEGG; ani:AN7900.2; -. DR HOGENOM; HOG000161810; -. DR OMA; RNTWYTG; -. DR OrthoDB; EOG092C281O; -. DR Proteomes; UP000000560; Chromosome II. DR Proteomes; UP000005890; Unassembled WGS sequence. DR GO; GO:0016491; F:oxidoreductase activity; IBA:GO_Central. DR Gene3D; 3.50.50.60; -; 1. DR InterPro; IPR036188; FAD/NAD-bd_sf. DR SUPFAM; SSF51905; SSF51905; 1. PE 2: Evidence at transcript level; KW FAD; Flavoprotein; Glycoprotein; Oxidoreductase; Reference proteome; KW Signal. FT SIGNAL 1..20 FT /evidence="ECO:0000255" FT CHAIN 21..467 FT /note="FAD-dependent oxidoreductase dbaF" FT /evidence="ECO:0000255" FT /id="PRO_5010288538" FT CARBOHYD 96 FT /note="N-linked (GlcNAc...) asparagine" FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498" FT CARBOHYD 134 FT /note="N-linked (GlcNAc...) asparagine" FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498" FT CARBOHYD 337 FT /note="N-linked (GlcNAc...) asparagine" FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498" FT CARBOHYD 391 FT /note="N-linked (GlcNAc...) asparagine" FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498" FT CARBOHYD 451 FT /note="N-linked (GlcNAc...) asparagine" FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498" SQ SEQUENCE 467 AA; 51478 MW; 33086CC663EDE630 CRC64; MKSVLASGAL TLAFSLAALA ADAFQASSWD STHIIRRDVV IVGGGAAGTY AAIRLKDHGK SVVLVERRDR LGGHAVTYKD PNTGGSVDYG VQVYDNNTVV RDFFSRLNTP LADLSFASFG KPVYADFEEG MLLNLTAGTL GQDYINELNK YPYLDNGFEL PDPVPEDLLL PWVEYIGKYN IDLSTAIATL ARPAVTGNLL NILAIYVFNN LNHLLLHEMS GAVVVNANRD NSQLYRNAVS ELQPDLLLRS RVVAGQRRTR KRDGVRLVVD TPTGRKLIIA KQLIVGMPPI LDNMRTFGLD SHEHSVLSHI YGLPYYGGVV SDTGLAPGFS FKNYAANTSY NLAEIPSVVA FNPSSVDGLF YYWYNAPQPV SQRRIETEAR DAIKTLQRLT NSTTQPEPKF LAFSDFAPYQ LRVSAEAIRN GFYDDMYGLQ GHRNTWYTGT LFVTGSSQVW NNTEVMLPEI LAAVNSS //