ID GCTB_ACIFE Reviewed; 266 AA. AC Q59112; DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot. DT 23-JAN-2007, sequence version 3. DT 20-MAR-2007, entry version 43. DE Glutaconate CoA-transferase subunit B (EC 2.8.3.12) (GCT small DE subunit). GN Name=gctB; OS Acidaminococcus fermentans. OC Bacteria; Firmicutes; Clostridia; Clostridiales; Acidaminococcaceae; OC Acidaminococcus. OX NCBI_TaxID=905; RN [1] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 2-19 AND RP 44-66. RC STRAIN=ATCC 25085 / VR4 / DSM 20731 / CIP 106432; RX MEDLINE=95045599; PubMed=7957258; RA Mack M., Bendrat K., Zelder O., Eckel E., Linder D., Buckel W.; RT "Location of the two genes encoding glutaconate coenzyme A-transferase RT at the beginning of the hydroxyglutarate operon in Acidaminococcus RT fermentans."; RL Eur. J. Biochem. 226:41-51(1994). RN [2] RP X-RAY CRYSTALLOGRAPHY (2.55 ANGSTROMS). RC STRAIN=ATCC 25085 / VR4 / DSM 20731 / CIP 106432; RX MEDLINE=97238937; PubMed=9083111; DOI=10.1016/S0969-2126(97)00198-6; RA Jacob U., Mack M., Clausen T., Huber R., Buckel W., Messerschmidt A.; RT "Glutaconate CoA-transferase from Acidaminococcus fermentans: the RT crystal structure reveals homology with other CoA-transferases."; RL Structure 5:415-426(1997). CC -!- FUNCTION: Catalyzes the transfer of the CoA moiety from acetyl-CoA CC to (R)-2-hydroxyglutarate and related compounds like glutaconate. CC -!- CATALYTIC ACTIVITY: Acetyl-CoA + (E)-glutaconate = acetate + CC glutaconyl-1-CoA. CC -!- PATHWAY: Glutamate fermentation. CC -!- SUBUNIT: Heterooctamer of four A and four B subunits. CC -!- SUBCELLULAR LOCATION: Cytoplasm. CC -!- SIMILARITY: Belongs to the glutaconate CoA-transferase subunit B CC family. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; X81440; CAA57200.1; -; Genomic_DNA. DR PIR; S51052; S51052. DR PDB; 1POI; X-ray; B/D=3-262. DR DIP; DIP:6201N; -. DR IntAct; Q59112; -. DR BioCyc; MetaCyc:MONOMER-1029; -. DR LinkHub; Q59112; -. DR GO; GO:0018730; F:glutaconate CoA-transferase activity; IEA:EC. DR InterPro; IPR004165; CoA_trans. DR Pfam; PF01144; CoA_trans; 1. KW 3D-structure; Direct protein sequencing; Transferase. FT INIT_MET 1 1 Removed. FT CHAIN 2 266 Glutaconate CoA-transferase subunit B. FT /FTId=PRO_0000157930. FT ACT_SITE 54 54 FT HELIX 8 17 FT STRAND 25 27 FT HELIX 32 42 FT STRAND 49 52 FT TURN 53 55 FT STRAND 56 59 FT HELIX 71 74 FT STRAND 76 80 FT HELIX 83 96 FT STRAND 101 105 FT STRAND 108 110 FT STRAND 119 122 FT STRAND 124 130 FT HELIX 137 143 FT STRAND 146 149 FT TURN 154 156 FT HELIX 175 178 FT STRAND 187 192 FT STRAND 195 199 FT TURN 201 203 FT STRAND 206 211 FT HELIX 217 222 FT HELIX 241 249 SQ SEQUENCE 266 AA; 29166 MW; 1E691B864237A784 CRC64; MADYTNYTNK EMQAVTIAKQ IKNGQVVTVG TGLPLIGASV AKRVYAPDCH IIVESGLMDC SPVEVPRSVG DLRFMAHCGC IWPNVRFVGF EINEYLHKAN RLIAFIGGAQ IDPYGNVNST SIGDYHHPKT RFTGSGGANG IATYSNTIIM MQHEKRRFMN KIDYVTSPGW IDGPGGRERL GLPGDVGPQL VVTDKGILKF DEKTKRMYLA AYYPTSSPED VLENTGFDLD VSKAVELEAP DPAVIKLIRE EIDPGQAFIQ VPTEAK //