ID GCTB_ACIFE Reviewed; 266 AA. AC Q59112; DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot. DT 23-JAN-2007, sequence version 3. DT 23-JAN-2007, entry version 42. DE Glutaconate CoA-transferase subunit B (EC 2.8.3.12) (GCT small DE subunit). GN Name=gctB; OS Acidaminococcus fermentans. OC Bacteria; Firmicutes; Clostridia; Clostridiales; Acidaminococcaceae; OC Acidaminococcus. OX NCBI_TaxID=905; RN [1] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 2-19 AND RP 44-66. RC STRAIN=ATCC 25085 / VR4 / DSM 20731 / CIP 106432; RX MEDLINE=95045599; PubMed=7957258; RA Mack M., Bendrat K., Zelder O., Eckel E., Linder D., Buckel W.; RT "Location of the two genes encoding glutaconate coenzyme A-transferase RT at the beginning of the hydroxyglutarate operon in Acidaminococcus RT fermentans."; RL Eur. J. Biochem. 226:41-51(1994). RN [2] RP X-RAY CRYSTALLOGRAPHY (2.55 ANGSTROMS). RC STRAIN=ATCC 25085 / VR4 / DSM 20731 / CIP 106432; RX MEDLINE=97238937; PubMed=9083111; DOI=10.1016/S0969-2126(97)00198-6; RA Jacob U., Mack M., Clausen T., Huber R., Buckel W., Messerschmidt A.; RT "Glutaconate CoA-transferase from Acidaminococcus fermentans: the RT crystal structure reveals homology with other CoA-transferases."; RL Structure 5:415-426(1997). CC -!- FUNCTION: Catalyzes the transfer of the CoA moiety from acetyl-CoA CC to (R)-2-hydroxyglutarate and related compounds like glutaconate. CC -!- CATALYTIC ACTIVITY: Acetyl-CoA + (E)-glutaconate = acetate + CC glutaconyl-1-CoA. CC -!- PATHWAY: Glutamate fermentation. CC -!- SUBUNIT: Heterooctamer of four A and four B subunits. CC -!- SUBCELLULAR LOCATION: Cytoplasm. CC -!- SIMILARITY: Belongs to the glutaconate CoA-transferase subunit B CC family. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; X81440; CAA57200.1; -; Genomic_DNA. DR PIR; S51052; S51052. DR PDB; 1POI; X-ray; B/D=3-262. DR DIP; DIP:6201N; -. DR IntAct; Q59112; -. DR BioCyc; MetaCyc:MONOMER-1029; -. DR LinkHub; Q59112; -. DR GO; GO:0018730; F:glutaconate CoA-transferase activity; IEA:EC. DR InterPro; IPR004165; CoA_trans. DR Pfam; PF01144; CoA_trans; 1. KW 3D-structure; Direct protein sequencing; Transferase. FT INIT_MET 1 1 Removed. FT CHAIN 2 266 Glutaconate CoA-transferase subunit B. FT /FTId=PRO_0000157930. FT ACT_SITE 54 54 FT HELIX 9 18 FT TURN 19 20 FT TURN 23 24 FT STRAND 26 28 FT HELIX 33 43 FT TURN 44 45 FT TURN 47 48 FT STRAND 50 53 FT TURN 54 56 FT STRAND 57 60 FT TURN 69 70 FT HELIX 72 75 FT TURN 76 76 FT STRAND 77 81 FT HELIX 84 97 FT TURN 98 98 FT STRAND 102 106 FT STRAND 109 111 FT TURN 113 114 FT STRAND 120 123 FT STRAND 125 131 FT TURN 136 137 FT HELIX 138 144 FT STRAND 147 150 FT TURN 155 157 FT TURN 171 172 FT TURN 174 175 FT HELIX 176 179 FT TURN 180 181 FT TURN 184 185 FT STRAND 188 193 FT TURN 194 195 FT STRAND 196 200 FT TURN 202 204 FT STRAND 207 212 FT TURN 214 215 FT HELIX 218 223 FT TURN 224 224 FT TURN 232 233 FT HELIX 242 250 FT TURN 251 252 FT TURN 254 255 SQ SEQUENCE 266 AA; 29166 MW; 1E691B864237A784 CRC64; MADYTNYTNK EMQAVTIAKQ IKNGQVVTVG TGLPLIGASV AKRVYAPDCH IIVESGLMDC SPVEVPRSVG DLRFMAHCGC IWPNVRFVGF EINEYLHKAN RLIAFIGGAQ IDPYGNVNST SIGDYHHPKT RFTGSGGANG IATYSNTIIM MQHEKRRFMN KIDYVTSPGW IDGPGGRERL GLPGDVGPQL VVTDKGILKF DEKTKRMYLA AYYPTSSPED VLENTGFDLD VSKAVELEAP DPAVIKLIRE EIDPGQAFIQ VPTEAK //