ID GCTB_ACIFE STANDARD; PRT; 265 AA. AC Q59112; DT 15-JUL-1998 (Rel. 36, Created) DT 30-MAY-2000 (Rel. 39, Last sequence update) DT 29-MAR-2004 (Rel. 43, Last annotation update) DE Glutaconate CoA-transferase subunit B (EC 2.8.3.12) (GCT small DE subunit). GN GCTB. OS Acidaminococcus fermentans. OC Bacteria; Firmicutes; Clostridia; Clostridiales; Acidaminococcaceae; OC Acidaminococcus. OX NCBI_TaxID=905; RN [1] RP SEQUENCE FROM N.A., AND SEQUENCE OF 1-18 AND 43-65. RC STRAIN=ATCC 25085; RX MEDLINE=95045599; PubMed=7957258; RA Mack M., Bendrat K., Zelder O., Eckel E., Linder D., Buckel W.; RT "Location of the two genes encoding glutaconate coenzyme RT A-transferase at the beginning of the hydroxyglutarate operon in RT Acidaminococcus fermentans."; RL Eur. J. Biochem. 226:41-51(1994). RN [2] RP X-RAY CRYSTALLOGRAPHY (2.55 ANGSTROMS). RC STRAIN=ATCC 25085; RX MEDLINE=97238937; PubMed=9083111; RA Jacob U., Mack M., Clausen T., Huber R., Buckel W., Messerschmidt A.; RT "Glutaconate CoA-transferase from Acidaminococcus fermentans: the RT crystal structure reveals homology with other CoA-transferases."; RL Structure 5:415-426(1997). CC -!- FUNCTION: Catalyzes the transfer of the CoA moiety from acetyl-CoA CC to (R)-2-hydroxyglutarate and related compounds like glutaconate. CC -!- CATALYTIC ACTIVITY: Acetyl-CoA + (E)-glutaconate = acetate + CC glutaconyl-1-CoA. CC -!- PATHWAY: Glutamate fermentation. CC -!- SUBUNIT: Heterooctamer of four A and four B subunits. CC -!- SUBCELLULAR LOCATION: Cytoplasmic. CC -!- SIMILARITY: Belongs to the glutaconate CoA-transferase subunit B CC family. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; X81440; CAA57200.1; -. DR PIR; S51052; S51052. DR PDB; 1POI; 18-MAR-98. KW Transferase; 3D-structure. FT INIT_MET 0 0 FT ACT_SITE 53 53 FT HELIX 8 17 FT TURN 18 19 FT TURN 22 23 FT STRAND 25 27 FT HELIX 32 42 FT TURN 43 44 FT TURN 46 47 FT STRAND 49 52 FT TURN 53 55 FT STRAND 56 59 FT TURN 68 69 FT HELIX 71 74 FT TURN 75 75 FT STRAND 77 80 FT HELIX 83 96 FT TURN 97 97 FT STRAND 101 105 FT STRAND 109 110 FT TURN 112 113 FT STRAND 116 117 FT STRAND 120 122 FT STRAND 127 130 FT TURN 135 136 FT HELIX 137 143 FT STRAND 146 149 FT TURN 154 156 FT STRAND 157 157 FT STRAND 166 166 FT TURN 170 171 FT TURN 173 174 FT HELIX 175 178 FT TURN 179 180 FT TURN 183 184 FT STRAND 187 192 FT TURN 193 194 FT STRAND 195 199 FT TURN 201 203 FT STRAND 206 211 FT TURN 213 214 FT HELIX 217 222 FT TURN 223 223 FT TURN 231 232 FT STRAND 234 235 FT HELIX 241 249 FT TURN 250 251 FT TURN 253 254 SQ SEQUENCE 265 AA; 29035 MW; 1E7FF61B42162FB4 CRC64; ADYTNYTNKE MQAVTIAKQI KNGQVVTVGT GLPLIGASVA KRVYAPDCHI IVESGLMDCS PVEVPRSVGD LRFMAHCGCI WPNVRFVGFE INEYLHKANR LIAFIGGAQI DPYGNVNSTS IGDYHHPKTR FTGSGGANGI ATYSNTIIMM QHEKRRFMNK IDYVTSPGWI DGPGGRERLG LPGDVGPQLV VTDKGILKFD EKTKRMYLAA YYPTSSPEDV LENTGFDLDV SKAVELEAPD PAVIKLIREE IDPGQAFIQV PTEAK //