ID   NQRA_VIBAL     STANDARD;      PRT;   446 AA.
AC   Q56586;
DT   16-OCT-2001 (Rel. 40, Created)
DT   16-OCT-2001 (Rel. 40, Last sequence update)
DT   01-MAY-2005 (Rel. 47, Last annotation update)
DE   Na(+)-translocating NADH-quinone reductase subunit A (EC 1.6.5.-)
DE   (Na(+)-translocating NADH-quinone reductase subunit alpha) (Na(+)-
DE   translocating NQR subunit A) (Na(+)-NQR subunit A) (NQR complex
DE   subunit A) (NQR-1 subunit A).
GN   Name=nqrA; Synonyms=nqr1;
OS   Vibrio alginolyticus.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales;
OC   Vibrionaceae; Vibrio.
OX   NCBI_TaxID=663;
RN   [1]
RP   NUCLEOTIDE SEQUENCE, AND PROTEIN SEQUENCE OF 1-10.
RC   STRAIN=NCIMB 11038;
RX   MEDLINE=95104445; PubMed=7805867; DOI=10.1016/0014-5793(94)01275-X;
RA   Beattie P., Tan K., Bourne R.M., Leach D.R.F., Rich P.R., Ward F.B.;
RT   "Cloning and sequencing of four structural genes for the Na(+)-
RT   translocating NADH-ubiquinone oxidoreductase of Vibrio
RT   alginolyticus.";
RL   FEBS Lett. 356:333-338(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE.
RA   Hayashi M., Unemoto T., Sugiyama A.;
RL   Submitted (OCT-1997) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   PROTEIN SEQUENCE OF 1-10.
RX   MEDLINE=98149659; PubMed=9490015; DOI=10.1016/S0014-5793(98)00016-7;
RA   Nakayama Y., Hayashi M., Unemoto T.;
RT   "Identification of six subunits constituting Na+-translocating NADH-
RT   quinone reductase from the marine Vibrio alginolyticus.";
RL   FEBS Lett. 422:240-242(1998).
RN   [4]
RP   PROTEIN SEQUENCE OF 1-9 AND 334-340.
RX   MEDLINE=95104444; PubMed=7805866; DOI=10.1016/0014-5793(94)01274-1;
RA   Hayashi M., Hirai K., Unemoto T.;
RT   "Cloning of the Na(+)-translocating NADH-quinone reductase gene from
RT   the marine bacterium Vibrio alginolyticus and the expression of the
RT   beta-subunit in Escherichia coli.";
RL   FEBS Lett. 356:330-332(1994).
RN   [5]
RP   INHIBITION OF ENZYMATIC ACTIVITY.
RX   MEDLINE=20016049; PubMed=10549856;
RA   Nakayama Y., Hayashi M., Yoshikawa K., Mochida K., Unemoto T.;
RT   "Inhibitor studies of a new antibiotic, korormicin, 2-n-heptyl-4-
RT   hydroxyquinoline N-oxide and Ag+ toward the Na+-translocating NADH-
RT   quinone reductase from the marine Vibrio alginolyticus.";
RL   Biol. Pharm. Bull. 22:1064-1067(1999).
RN   [6]
RP   REVIEW.
RX   MEDLINE=21145117; PubMed=11248187; DOI=10.1016/S0005-2728(00)00275-9;
RA   Hayashi M., Nakayama Y., Unemoto T.;
RT   "Recent progress in the Na(+)-translocating NADH-quinone reductase
RT   from the marine Vibrio alginolyticus.";
RL   Biochim. Biophys. Acta 1505:37-44(2001).
RN   [7]
RP   REVIEW.
RX   MEDLINE=21145118; PubMed=11248188; DOI=10.1016/S0005-2728(00)00276-0;
RA   Steuber J.;
RT   "Na(+) translocation by bacterial NADH:quinone oxidoreductases: an
RT   extension to the complex-I family of primary redox pumps.";
RL   Biochim. Biophys. Acta 1505:45-56(2001).
CC   -!- FUNCTION: NQR complex catalyzes the reduction of ubiquinone-1 to
CC       ubiquinol by two successive reactions, coupled with the transport
CC       of Na(+) ions from the cytoplasm to the periplasm. NqrA to nqrE
CC       are probably involved in the second step, the conversion of
CC       ubisemiquinone to ubiquinol.
CC   -!- CATALYTIC ACTIVITY: NADH + ubiquinone + Na(+)(In) = NAD(+) +
CC       ubiquinol + Na(+)(Out).
CC   -!- ENZYME REGULATION: This reaction is tightly coupled to the Na(+)
CC       pumping activity and specifically requires Na(+) for activity.
CC       Inhibited by korormicin and 2-N-heptyl-4-hydroxyquinoline N-oxide
CC       (HQNO).
CC   -!- SUBUNIT: Composed of six subunits; nqrA, nqrB, nqrC, nqrD, nqrE
CC       and nqrF.
CC   -!- SIMILARITY: Belongs to the nqrA family.
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DR   EMBL; Z37111; CAA85476.1; -.
DR   EMBL; AB008030; BAA22910.1; -.
DR   PIR; S51015; S51015.
DR   HAMAP; MF_00425; -; 1.
DR   InterPro; IPR011053; Hybrid_motif.
DR   InterPro; IPR008703; NQRA.
DR   Pfam; PF05896; NQRA; 1.
DR   TIGRFAMs; TIGR01936; nqrA; 1.
KW   Direct protein sequencing; Ion transport; NAD; Oxidoreductase; Sodium;
KW   Sodium transport; Transport; Ubiquinone.
FT   CONFLICT    337    337       W -> L (in Ref. 4).
SQ   SEQUENCE   446 AA;  48623 MW;  6D65ACAA53FE515C CRC64;
     MITIKKGLDL PIAGTPSQVI NDGKTIKKVA LLGEEYVGMR PTMHVRVGDE VKKAQVLFED
     KKNPGVKFTA PAAGKVIEVN RGAKRVLQSV VIEVAGEEQV TFDKFEAAQL SGLDREVIKT
     QLVDSGLWTA LRTRPFSKVP AIESSTKAIF VTAMDTNPLA AKPELIINEQ QEAFIAGLDI
     LSALTEGKVY VCKSGTSLPR SSQSNVEEHV FDGPHPAGLA GTHMHFLYPV NAENVAWSIN
     YQDVIAFGKL FLTGELYTDR VVSLAGPVVN NPRLVRTVIG ASLDDLTDNE LMPGEVRVIS
     GSVLTGTHAT GPHAYLGRYH QQVSVLREGR EKELFGWAMP GKNKFSVTRS FLGHVFKGQL
     FNMTTTTNGS DRSMVPIGNY ERVMPLDMEP TLLLRDLCAG DTDSAQALGA LELDEEDLAL
     CTFVCPGKYE YGTLLRECLD TIEKEG
//