ID DGAT1_DICDI Reviewed; 617 AA. AC Q55BH9; DT 16-JUN-2009, integrated into UniProtKB/Swiss-Prot. DT 16-JUN-2009, sequence version 2. DT 11-DEC-2019, entry version 88. DE RecName: Full=Diacylglycerol O-acyltransferase 1; DE EC=2.3.1.20; GN Name=dgat1; ORFNames=DDB_G0271342; OS Dictyostelium discoideum (Slime mold). OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelids; Dictyosteliales; OC Dictyosteliaceae; Dictyostelium. OX NCBI_TaxID=44689; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=AX4; RX PubMed=12097910; DOI=10.1038/nature00847; RA Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T., RA Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R., RA Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A., RA Noegel A.A.; RT "Sequence and analysis of chromosome 2 of Dictyostelium discoideum."; RL Nature 418:79-85(2002). RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=AX4; RX PubMed=15875012; DOI=10.1038/nature03481; RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G., RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.; RT "The genome of the social amoeba Dictyostelium discoideum."; RL Nature 435:43-57(2005). CC -!- FUNCTION: Catalyzes the terminal and only committed step in CC triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as CC substrates. {ECO:0000250}. CC -!- CATALYTIC ACTIVITY: CC Reaction=a 1,2-diacyl-sn-glycerol + an acyl-CoA = a triacyl-sn-glycerol CC + CoA; Xref=Rhea:RHEA:10868, ChEBI:CHEBI:17815, ChEBI:CHEBI:57287, CC ChEBI:CHEBI:58342, ChEBI:CHEBI:64615; EC=2.3.1.20; CC -!- PATHWAY: Lipid metabolism; glycerolipid metabolism. CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000250}; CC Multi-pass membrane protein {ECO:0000250}. CC -!- SIMILARITY: Belongs to the membrane-bound acyltransferase family. CC Sterol o-acyltransferase subfamily. {ECO:0000305}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AAFI02000006; EAL71801.2; -; Genomic_DNA. DR RefSeq; XP_645633.2; XM_640541.2. DR STRING; 44689.DDB0304727; -. DR PaxDb; Q55BH9; -. DR EnsemblProtists; EAL71801; EAL71801; DDB_G0271342. DR GeneID; 8617825; -. DR KEGG; ddi:DDB_G0271342; -. DR dictyBase; DDB_G0271342; dgat1. DR eggNOG; KOG0380; Eukaryota. DR eggNOG; COG5056; LUCA. DR InParanoid; Q55BH9; -. DR KO; K11155; -. DR OMA; YFWLAPT; -. DR PhylomeDB; Q55BH9; -. DR Reactome; R-DDI-1482883; Acyl chain remodeling of DAG and TAG. DR Reactome; R-DDI-6798695; Neutrophil degranulation. DR Reactome; R-DDI-75109; Triglyceride biosynthesis. DR UniPathway; UPA00230; -. DR PRO; PR:Q55BH9; -. DR Proteomes; UP000002195; Chromosome 2. DR Proteomes; UP000002195; Unassembled WGS sequence. DR GO; GO:0005789; C:endoplasmic reticulum membrane; IDA:dictyBase. DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW. DR GO; GO:0004144; F:diacylglycerol O-acyltransferase activity; IDA:dictyBase. DR GO; GO:0008611; P:ether lipid biosynthetic process; IDA:dictyBase. DR GO; GO:0006071; P:glycerol metabolic process; IEA:UniProtKB-KW. DR GO; GO:0140042; P:lipid droplet formation; IMP:dictyBase. DR GO; GO:0019432; P:triglyceride biosynthetic process; IDA:dictyBase. DR InterPro; IPR027251; Diacylglycerol_acylTrfase1. DR InterPro; IPR004299; MBOAT_fam. DR InterPro; IPR014371; Oat_ACAT_DAG_ARE. DR PANTHER; PTHR10408; PTHR10408; 1. DR PANTHER; PTHR10408:SF7; PTHR10408:SF7; 1. DR Pfam; PF03062; MBOAT; 1. DR PIRSF; PIRSF000439; Oat_ACAT_DAG_ARE; 1. PE 3: Inferred from homology; KW Acyltransferase; Endoplasmic reticulum; Glycerol metabolism; KW Lipid biosynthesis; Lipid metabolism; Membrane; Reference proteome; KW Transferase; Transmembrane; Transmembrane helix. FT CHAIN 1..617 FT /note="Diacylglycerol O-acyltransferase 1" FT /id="PRO_0000377488" FT TRANSMEM 217..237 FT /note="Helical" FT /evidence="ECO:0000255" FT TRANSMEM 254..274 FT /note="Helical" FT /evidence="ECO:0000255" FT TRANSMEM 306..326 FT /note="Helical" FT /evidence="ECO:0000255" FT TRANSMEM 399..419 FT /note="Helical" FT /evidence="ECO:0000255" FT TRANSMEM 449..469 FT /note="Helical" FT /evidence="ECO:0000255" FT TRANSMEM 520..540 FT /note="Helical" FT /evidence="ECO:0000255" FT TRANSMEM 545..565 FT /note="Helical" FT /evidence="ECO:0000255" FT TRANSMEM 570..590 FT /note="Helical" FT /evidence="ECO:0000255" FT COMPBIAS 18..35 FT /note="Poly-Gln" FT COMPBIAS 96..104 FT /note="Poly-Asn" FT COMPBIAS 153..158 FT /note="Poly-His" FT COMPBIAS 159..177 FT /note="Poly-Asn" FT ACT_SITE 532 FT /evidence="ECO:0000255" SQ SEQUENCE 617 AA; 71946 MW; DCD8FD193A3023D7 CRC64; MEPIPPSNGN KNNSMDKQPQ QPQQPQQQQQ QQQQQRRDQR NSKLNELNET ERVRNRFISH EFHKLDRTKS RIDAPKISFS DSESESDSEF FLAKRNTNNN NQNNTSPTFS SANGKQSNLT QRKINTQIQS KQPTNNNVQP LTDDEGTINH SNHHHHHHNQ NNNGNNNNNN NNNNNNNKIS TPPKQEEKMT MNGLFTLRPS ILSSESNGSS YRGFLNLLLI LLITASFRLV ILNHLLYGIR INLDLYKISE YHRWPGVMIS LMINLFIIAA YLIEKAAAKQ LLPDRICYLL RIINCAAVII VPSGSIIAFS PNPASGIIVM ILICTFSMKI ISYAYENSKQ RKLNPDNKKF VIDPTNTSIY PNNLSLRSTY WFMLVPTLVY QLSYPRSPKI RKGYLLRRIV EALSLSLLIL WMVNQYMLPL VQNSIEPLEK IDIVLIVERI MKLSLPNLYV WLLGFYVFFH LYLNIVAEIT RFGDREFYRD WWNSTGLDYF WRTWNMPVHH WMVVLIYTPM RRRGFSKNMG YFMCFFVSAI FHELVISIPF HSLKLWGFFG IMSQMVLIAL TKNLMNGRNL GNVIFWISIV LGQPLVVLLY YRNFVLENPE WYRNVEPPTS PPVMPFY //